(Circular) (Six-base) MAPSORT of: pFN21ASDA0908.seq Check: 8909 from: 1 to: 6244 pFN21ASDA0908 6244 bp With 182 enzymes: SgfI * January 16, 2009 15:30 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4054 278 Size: 53 83 186 936 2518 2468 Fragments arranged by size: 2518 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2910 3587 1937 Size: 973 677 4594 Fragments arranged by size: 4594 973 677 Acc65I G'GTAC_C Cuts at: 3565 3565 Size: 6244 AclI AA'CG_TT Cuts at: 1563 2323 4371 4744 1563 Size: 760 2048 373 3063 Fragments arranged by size: 3063 2048 760 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2148 2533 4307 5691 5959 1632 Size: 516 385 1774 1384 268 1917 Fragments arranged by size: 1917 1774 1384 516 385 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6047 AflIII A'CryG_T Cuts at: 1203 2481 2496 5143 6120 1203 Size: 1278 15 2647 977 1327 Fragments arranged by size: 2647 1327 1278 977 15 AgeI A'CCGG_T Cuts at: 5075 5075 Size: 6244 AhdI GACnn_n'nnGTC Cuts at: 4973 4973 Size: 6244 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6244 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6244 AlwNI CAG_nnn'CTG Cuts at: 3987 5559 3987 Size: 1572 4672 ApaLI G'TGCA_C Cuts at: 4301 5457 4301 Size: 1156 5088 ApoI r'AATT_y Cuts at: 1493 2189 3026 3113 3273 3553 3779 3931 6040 Size: 696 837 87 160 280 226 152 2109 Cuts at: 6040 1493 Size: 1697 Fragments arranged by size: 2109 1697 837 696 280 226 160 152 87 AseI AT'TA_AT Cuts at: 160 1032 4798 160 Size: 872 3766 1606 Fragments arranged by size: 3766 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6244 AvaI C'yCGr_G Cuts at: 1870 1943 2096 3569 1870 Size: 73 153 1473 4545 Fragments arranged by size: 4545 1473 153 73 BamHI G'GATC_C Cuts at: 3574 3941 3574 Size: 367 5877 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2102 3565 5020 618 Size: 325 217 627 315 1463 1455 1842 Fragments arranged by size: 1842 1463 1455 627 325 315 217 BanII G_rGCy'C Cuts at: 1391 2046 3563 1391 Size: 655 1517 4072 Fragments arranged by size: 4072 1517 655 BbeI G_GCGC'C Cuts at: 2106 2106 Size: 6244 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5273 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2096 2130 4435 4469 1903 Size: 34 159 34 2305 34 3678 Fragments arranged by size: 3678 2305 159 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4136 5346 4136 Size: 1210 5034 BclI T'GATC_A Cuts at: 1548 3499 1548 Size: 1951 4293 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2100 4855 136 Size: 107 122 71 631 605 428 2755 1525 Fragments arranged by size: 2755 1525 631 605 428 122 107 71 BglII A'GATC_T Cuts at: 3240 6239 3240 Size: 2999 3245 BlpI GC'TnA_GC Cuts at: 3661 3661 Size: 6244 Bme1580I G_kGCm'C Cuts at: 1790 3183 3486 4305 5461 1790 Size: 1393 303 819 1156 2573 Fragments arranged by size: 2573 1393 1156 819 303 BmgBI CAC'GTC Cuts at: 1318 2497 1318 Size: 1179 5065 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2384 3975 4933 Size: 416 53 83 399 24 967 1591 958 Cuts at: 4933 442 Size: 1753 Fragments arranged by size: 1753 1591 967 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6244 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2065 2065 Size: 6244 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4904 1239 Size: 3665 2579 Bpu10I CC'TnA_GC Cuts at: 2797 3207 2797 Size: 410 5834 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4369 5234 5532 4369 Size: 865 298 5081 Fragments arranged by size: 5081 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2348 4907 882 Size: 617 849 2559 2219 Fragments arranged by size: 2559 2219 849 617 BsaAI yAC'GTr Cuts at: 493 1204 3541 4035 493 Size: 711 2337 494 2702 Fragments arranged by size: 2702 2337 711 494 BsaBI GATnn'nnATC Cuts at: 6238 6238 Size: 6244 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2103 4051 4433 5068 Size: 53 83 186 936 570 1948 382 635 Cuts at: 5068 275 Size: 1451 Fragments arranged by size: 1948 1451 936 635 570 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4677 5075 5349 5496 5907 4677 Size: 398 274 147 411 5014 Fragments arranged by size: 5014 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2277 2307 1178 Size: 30 165 30 874 30 5115 Fragments arranged by size: 5115 874 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2041 2423 2598 2870 1186 Size: 855 382 175 272 4560 Fragments arranged by size: 4560 855 382 272 175 BseYI C'CCAG_C Cuts at: 2059 5447 5943 2059 Size: 3388 496 2360 Fragments arranged by size: 3388 2360 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2101 2282 2297 3429 3438 1163 Size: 573 365 181 15 1132 9 3969 Fragments arranged by size: 3969 1132 573 365 181 15 9 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3694 4455 5483 1540 Size: 275 186 1693 761 1028 2301 Fragments arranged by size: 2301 1693 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2046 2119 4305 4390 5461 2046 Size: 73 2186 85 1071 2829 Fragments arranged by size: 2829 2186 1071 85 73 BsmI GAATG_Cn' Cuts at: 2607 3755 3848 2607 Size: 1148 93 5003 Fragments arranged by size: 5003 1148 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2053 2053 Size: 6244 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2046 2119 3183 3486 3563 4305 4390 Size: 399 256 73 1064 303 77 742 85 Cuts at: 4390 5461 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 1064 742 399 303 256 85 77 73 BspEI T'CCGG_A Cuts at: 5907 5907 Size: 6244 BspHI T'CATG_A Cuts at: 2511 2536 4133 5863 2511 Size: 25 1597 1730 2892 Fragments arranged by size: 2892 1730 1597 25 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2187 3599 844 Size: 1343 1412 3489 Fragments arranged by size: 3489 1412 1343 BsrBI CCG'CTC Cuts at: 1394 1823 4131 1394 Size: 429 2308 3507 Fragments arranged by size: 3507 2308 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3435 4739 4913 65 Size: 1183 2187 1304 174 1396 Fragments arranged by size: 2187 1396 1304 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2639 4888 5075 1700 Size: 110 829 2249 187 2869 Fragments arranged by size: 2869 2249 829 187 110 BsrGI T'GTAC_A Cuts at: 96 2847 96 Size: 2751 3493 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6244 BssSI C'ACGA_G Cuts at: 4304 5316 4304 Size: 1012 5232 BstAPI GCAn_nnn'nTGC Cuts at: 794 3416 3461 794 Size: 2622 45 3577 Fragments arranged by size: 3577 2622 45 BstBI TT'CG_AA Cuts at: 2007 5048 2007 Size: 3041 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6244 BstYI r'GATC_y Cuts at: 1972 2517 3202 3240 3574 3941 4326 4343 5795 Size: 545 685 38 334 367 385 17 1452 Cuts at: 5795 5881 5893 6239 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 685 545 385 367 346 334 86 38 17 12 BtgI C'CryG_G Cuts at: 513 1065 2022 3317 3420 513 Size: 552 957 1295 103 3337 Fragments arranged by size: 3337 1295 957 552 103 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5505 Fragments arranged by size: 5505 683 56 BtsI GCAGTG_nn' Cuts at: 3760 4554 4574 3760 Size: 794 20 5430 Fragments arranged by size: 5430 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6244 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6209 DraI TTT'AAA Cuts at: 2307 3279 3548 2307 Size: 972 269 5003 Fragments arranged by size: 5003 972 269 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5251 809 Size: 507 3935 1802 Fragments arranged by size: 3935 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2698 2961 3691 Size: 54 1425 50 275 18 868 263 730 Cuts at: 3691 4580 6209 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 868 730 275 263 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3691 1537 Size: 275 1879 4090 Fragments arranged by size: 4090 1879 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2223 4174 1353 Size: 870 1951 3423 Fragments arranged by size: 3423 1951 870 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2109 4831 5205 5351 387 Size: 1064 138 520 2722 374 146 1280 Fragments arranged by size: 2722 1280 1064 520 374 146 138 EcoICRI GAG'CTC Cuts at: 2044 2044 Size: 6244 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2148 2533 4307 4904 5691 5959 1239 Size: 393 516 385 1774 597 787 268 1524 Fragments arranged by size: 1774 1524 787 597 516 393 385 268 EcoRI G'AATT_C Cuts at: 3026 3553 3931 3026 Size: 527 378 5339 Fragments arranged by size: 5339 527 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2659 3275 2659 Size: 616 5628 FspI TGC'GCA Cuts at: 4750 4750 Size: 6244 HaeII r_GCGC'y Cuts at: 1678 2106 2455 5391 1678 Size: 428 349 2936 2531 Fragments arranged by size: 2936 2531 428 349 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2217 2249 2277 2309 4885 Size: 32 135 32 645 32 28 32 2576 Cuts at: 4885 4917 4959 4991 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2576 645 135 42 32 32 32 32 32 32 28 HincII GTy'rAC Cuts at: 669 1657 1938 3588 3834 669 Size: 988 281 1650 246 3079 Fragments arranged by size: 3079 1650 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6244 HpaI GTT'AAC Cuts at: 3834 3834 Size: 6244 KasI G'GCGC_C Cuts at: 2102 2102 Size: 6244 KpnI G_GTAC'C Cuts at: 3569 3569 Size: 6244 MfeI C'AATT_G Cuts at: 3018 3843 3018 Size: 825 5419 MluI A'CGCG_T Cuts at: 6120 6120 Size: 6244 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2912 5358 5542 1305 Size: 1607 2446 184 2007 Fragments arranged by size: 2446 2007 1607 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2700 2963 10 Size: 54 1425 343 868 263 3291 Fragments arranged by size: 3291 1425 868 343 263 54 MslI CAynn'nnrTG Cuts at: 518 1166 2258 2541 3425 3491 4202 4561 4720 Size: 648 1092 283 884 66 711 359 159 Cuts at: 4720 518 Size: 2042 Fragments arranged by size: 2042 1092 884 711 648 359 283 159 66 MspA1I CmG'CkG Cuts at: 1606 2085 3474 3660 4337 5485 5730 1606 Size: 479 1389 186 677 1148 245 2120 Fragments arranged by size: 2120 1389 1148 677 479 245 186 NaeI GCC'GGC Cuts at: 2641 2641 Size: 6244 NarI GG'CG_CC Cuts at: 2103 2103 Size: 6244 NcoI C'CATG_G Cuts at: 513 1065 2022 3317 3420 513 Size: 552 957 1295 103 3337 Fragments arranged by size: 3337 1295 957 552 103 NdeI CA'TA_TG Cuts at: 387 3229 387 Size: 2842 3402 NgoMIV G'CCGG_C Cuts at: 2639 2639 Size: 6244 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6244 NotI GC'GGCC_GC Cuts at: 3691 3691 Size: 6244 NruI TCG'CGA Cuts at: 2135 2135 Size: 6244 NspI r_CATG'y Cuts at: 1132 1229 2485 3602 5147 1132 Size: 97 1256 1117 1545 2229 Fragments arranged by size: 2229 1545 1256 1117 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6244 PciI A'CATG_T Cuts at: 2481 5143 2481 Size: 2662 3582 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6244 PmeI GTTT'AAAC Cuts at: 3548 3548 Size: 6244 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2914 2946 4313 4345 5826 5858 2914 Size: 32 1367 32 1481 32 3300 Fragments arranged by size: 3300 1481 1367 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 2340 1613 Size: 727 5517 PsiI TTA'TAA Cuts at: 3814 3814 Size: 6244 PstI C_TGCA'G Cuts at: 830 2811 3596 830 Size: 1981 785 3478 Fragments arranged by size: 3478 1981 785 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6244 PvuII CAG'CTG Cuts at: 2085 2085 Size: 6244 SacI G_AGCT'C Cuts at: 2046 2046 Size: 6244 SalI G'TCGA_C Cuts at: 1936 3586 1936 Size: 1650 4594 SbfI CC_TGCA'GG Cuts at: 3596 3596 Size: 6244 ScaI AGT'ACT Cuts at: 4492 6204 4492 Size: 1712 4532 SfcI C'TryA_G Cuts at: 826 1045 2807 3592 4727 5408 5599 826 Size: 219 1762 785 1135 681 191 1471 Fragments arranged by size: 1762 1471 1135 785 681 219 191 SfoI GGC'GCC Cuts at: 2104 2104 Size: 6244 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6244 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6244 SmaI CCC'GGG Cuts at: 1872 3571 1872 Size: 1699 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4348 5249 5511 820 Size: 197 926 2405 901 262 1553 Fragments arranged by size: 2405 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3541 4035 493 Size: 3048 494 2702 Fragments arranged by size: 3048 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 6244 SphI G_CATG'C Cuts at: 1132 3602 1132 Size: 2470 3774 SspI AAT'ATT Cuts at: 5 52 2992 3038 4168 5 Size: 47 2940 46 1130 2081 Fragments arranged by size: 2940 2081 1130 47 46 StuI AGG'CCT Cuts at: 1517 2360 1517 Size: 843 5401 StyI C'CwwG_G Cuts at: 513 1065 2022 2596 3317 3420 3683 513 Size: 552 957 574 721 103 263 3074 Fragments arranged by size: 3074 957 721 574 552 263 103 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4271 4288 4441 4626 5071 325 Size: 1255 2691 17 153 185 445 1498 Fragments arranged by size: 2691 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2847 2874 4490 Size: 274 80 33 51 1443 870 27 1616 Cuts at: 4490 6202 96 Size: 1712 138 Fragments arranged by size: 1712 1616 1443 870 274 138 80 51 33 27 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2869 2901 6204 6236 2869 Size: 32 3303 32 2877 Fragments arranged by size: 3303 2877 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2951 1316 Size: 1635 4609 XbaI T'CTAG_A Cuts at: 3098 3580 3098 Size: 482 5762 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6244 XmaI C'CCGG_G Cuts at: 1870 3569 1870 Size: 1699 4545 XmnI GAAnn'nnTTC Cuts at: 4373 4373 Size: 6244 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4052 276 Size: 53 83 186 936 2518 2468 Fragments arranged by size: 2518 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI BfrBI BsiWI BstXI BstZ17I Bsu36I DraIII EcoNI EcoO109I EcoRV FseI FspAI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SrfI SwaI XcmI