(Circular) (Six-base) MAPSORT of: pFN21ASDA0899.seq Check: 4306 from: 1 to: 7546 pFN21ASDA0899 7546 bp With 182 enzymes: SgfI * January 16, 2009 15:30 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2613 3600 4360 4369 2613 Size: 987 760 9 5790 Fragments arranged by size: 5790 987 760 9 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2558 2738 5356 278 Size: 53 83 186 936 1022 180 2618 2468 Fragments arranged by size: 2618 2468 1022 936 186 180 83 53 AccI GT'mk_AC Cuts at: 1937 3980 4889 1937 Size: 2043 909 4594 Fragments arranged by size: 4594 2043 909 Acc65I G'GTAC_C Cuts at: 3405 4867 3405 Size: 1462 6084 AclI AA'CG_TT Cuts at: 1563 4540 5673 6046 1563 Size: 2977 1133 373 3063 Fragments arranged by size: 3063 2977 1133 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3170 3317 3464 4295 5609 6993 7261 1632 Size: 1538 147 147 831 1314 1384 268 1917 Fragments arranged by size: 1917 1538 1384 1314 831 268 147 147 AflII C'TTAA_G Cuts at: 820 1017 4202 820 Size: 197 3185 4164 Fragments arranged by size: 4164 3185 197 AflIII A'CryG_T Cuts at: 1203 4080 4464 6445 7422 1203 Size: 2877 384 1981 977 1327 Fragments arranged by size: 2877 1981 1327 977 384 AgeI A'CCGG_T Cuts at: 6377 6377 Size: 7546 AhdI GACnn_n'nnGTC Cuts at: 6275 6275 Size: 7546 AleI CACnn'nnGTG Cuts at: 1166 2625 1166 Size: 1459 6087 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3771 4085 1184 Size: 2587 314 4645 Fragments arranged by size: 4645 2587 314 AlwNI CAG_nnn'CTG Cuts at: 2840 3031 5289 6861 2840 Size: 191 2258 1572 3525 Fragments arranged by size: 3525 2258 1572 191 ApaI G_GGCC'C Cuts at: 3884 4043 3884 Size: 159 7387 ApaLI G'TGCA_C Cuts at: 2600 3079 5603 6759 2600 Size: 479 2524 1156 3387 Fragments arranged by size: 3387 2524 1156 479 ApoI r'AATT_y Cuts at: 1493 2150 4211 4222 4496 4681 4855 5081 5233 Size: 657 2061 11 274 185 174 226 152 Cuts at: 5233 7342 1493 Size: 2109 1697 Fragments arranged by size: 2109 2061 1697 657 274 226 185 174 152 11 AseI AT'TA_AT Cuts at: 160 1032 6100 160 Size: 872 5068 1606 Fragments arranged by size: 5068 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7546 AvaI C'yCGr_G Cuts at: 1870 1943 2592 3562 4013 4871 1870 Size: 73 649 970 451 858 4545 Fragments arranged by size: 4545 970 858 649 451 73 BamHI G'GATC_C Cuts at: 4876 5243 4876 Size: 367 7179 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3405 4417 4451 4867 6322 Size: 325 217 627 1618 1012 34 416 1455 Cuts at: 6322 618 Size: 1842 Fragments arranged by size: 1842 1618 1455 1012 627 416 325 217 34 BanII G_rGCy'C Cuts at: 1391 3884 4043 4865 1391 Size: 2493 159 822 4072 Fragments arranged by size: 4072 2493 822 159 BbeI G_GCGC'C Cuts at: 4421 4421 Size: 7546 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2059 3158 4137 928 Size: 971 160 1099 979 4337 Fragments arranged by size: 4337 1099 979 971 160 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5737 5771 1903 Size: 34 3800 34 3678 Fragments arranged by size: 3800 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3343 5438 6648 3343 Size: 2095 1210 4241 Fragments arranged by size: 4241 2095 1210 BclI T'GATC_A Cuts at: 1548 2355 2652 1548 Size: 807 297 6442 Fragments arranged by size: 6442 807 297 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3311 6157 136 Size: 107 122 71 631 605 1639 2846 1525 Fragments arranged by size: 2846 1639 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2749 7541 2749 Size: 4792 2754 BlpI GC'TnA_GC Cuts at: 2720 3248 3776 4963 2720 Size: 528 528 1187 5303 Fragments arranged by size: 5303 1187 528 528 Bme1580I G_kGCm'C Cuts at: 1790 2604 3083 3884 4043 4454 5607 6763 1790 Size: 814 479 801 159 411 1153 1156 2573 Fragments arranged by size: 2573 1156 1153 814 801 479 411 159 BmgBI CAC'GTC Cuts at: 1318 3166 3442 3703 3739 3934 4081 1318 Size: 1848 276 261 36 195 147 4783 Fragments arranged by size: 4783 1848 276 261 195 147 36 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 5277 6235 442 Size: 416 53 83 399 24 3860 958 1753 Fragments arranged by size: 3860 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7546 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2738 2891 3089 3278 3576 3836 3923 6206 Size: 1499 153 198 189 298 260 87 2283 Cuts at: 6206 1239 Size: 2579 Fragments arranged by size: 2579 2283 1499 298 260 198 189 153 87 Bpu10I CC'TnA_GC Cuts at: 3725 3948 3725 Size: 223 7323 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3851 5671 6536 6834 3851 Size: 1820 865 298 4563 Fragments arranged by size: 4563 1820 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2867 3038 3552 4016 6209 882 Size: 617 1368 171 514 464 2193 2219 Fragments arranged by size: 2219 2193 1368 617 514 464 171 BsaAI yAC'GTr Cuts at: 493 1204 3388 4843 5337 493 Size: 711 2184 1455 494 2702 Fragments arranged by size: 2702 2184 1455 711 494 BsaBI GATnn'nnATC Cuts at: 3425 4238 4671 7540 3425 Size: 813 433 2869 3431 Fragments arranged by size: 3431 2869 813 433 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2555 2735 4418 5353 Size: 53 83 186 936 1022 180 1683 935 Cuts at: 5353 5735 6370 275 Size: 382 635 1451 Fragments arranged by size: 1683 1451 1022 936 935 635 382 186 180 83 53 BsaWI w'CCGG_w Cuts at: 5979 6377 6651 6798 7209 5979 Size: 398 274 147 411 6316 Fragments arranged by size: 6316 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3062 3092 3830 3860 3973 Size: 30 165 30 1659 30 738 30 113 Cuts at: 3973 4003 1178 Size: 30 4721 Fragments arranged by size: 4721 1659 738 165 113 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3185 3833 4042 1186 Size: 1999 648 209 4690 Fragments arranged by size: 4690 1999 648 209 BseYI C'CCAG_C Cuts at: 3884 3958 4006 6749 7245 3884 Size: 74 48 2743 496 4185 Fragments arranged by size: 4185 2743 496 74 48 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2402 3464 3581 3637 4394 4493 4595 Size: 573 666 1062 117 56 757 99 102 Cuts at: 4595 4632 1163 Size: 37 4077 Fragments arranged by size: 4077 1062 757 666 573 117 102 99 56 37 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2031 3214 3810 4353 4996 5757 Size: 275 186 30 1183 596 543 643 761 Cuts at: 5757 6785 1540 Size: 1028 2301 Fragments arranged by size: 2301 1183 1028 761 643 596 543 275 186 30 BsiHKAI G_wGCw'C Cuts at: 2604 3083 5607 5692 6763 2604 Size: 479 2524 85 1071 3387 Fragments arranged by size: 3387 2524 1071 479 85 BsmI GAATG_Cn' Cuts at: 2944 5057 5150 2944 Size: 2113 93 5340 Fragments arranged by size: 5340 2113 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3125 3125 Size: 7546 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2604 3083 3884 4043 4454 4865 5607 Size: 399 814 479 801 159 411 411 742 Cuts at: 5607 5692 6763 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 814 801 742 479 411 411 399 159 85 BspEI T'CCGG_A Cuts at: 7209 7209 Size: 7546 BspHI T'CATG_A Cuts at: 2230 5435 7165 2230 Size: 3205 1730 2611 Fragments arranged by size: 3205 2611 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2274 2613 3003 3060 3600 3777 4011 4335 Size: 1430 339 390 57 540 177 234 324 Cuts at: 4335 4360 4369 4761 4901 844 Size: 25 9 392 140 3489 Fragments arranged by size: 3489 1430 540 392 390 339 324 234 177 140 57 25 9 BsrBI CCG'CTC Cuts at: 1394 1823 3160 3841 5433 1394 Size: 429 1337 681 1592 3507 Fragments arranged by size: 3507 1592 1337 681 429 BsrDI GCAATG_nn' Cuts at: 65 1248 6041 6215 65 Size: 1183 4793 174 1396 Fragments arranged by size: 4793 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2026 4770 6190 6377 1700 Size: 110 216 2744 1420 187 2869 Fragments arranged by size: 2869 2744 1420 216 187 110 BsrGI T'GTAC_A Cuts at: 96 2547 96 Size: 2451 5095 BssHII G'CGCG_C Cuts at: 1925 3633 4365 1925 Size: 1708 732 5106 Fragments arranged by size: 5106 1708 732 BssSI C'ACGA_G Cuts at: 3497 4073 5606 6618 3497 Size: 576 1533 1012 4425 Fragments arranged by size: 4425 1533 1012 576 BstAPI GCAn_nnn'nTGC Cuts at: 794 4573 794 Size: 3779 3767 BstBI TT'CG_AA Cuts at: 2007 6350 2007 Size: 4343 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7546 BstXI CCAn_nnnn'nTGG Cuts at: 2404 4342 2404 Size: 1938 5608 BstYI r'GATC_y Cuts at: 1972 2472 2749 3566 4876 5243 5628 5645 7097 Size: 500 277 817 1310 367 385 17 1452 Cuts at: 7097 7183 7195 7541 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 1310 817 500 385 367 346 277 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2478 2478 Size: 7546 BtgI C'CryG_G Cuts at: 513 1065 2574 3186 3783 4035 4341 4353 513 Size: 552 1509 612 597 252 306 12 3706 Fragments arranged by size: 3706 1509 612 597 552 306 252 12 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2410 487 Size: 683 56 1184 5623 Fragments arranged by size: 5623 1184 683 56 BtsI GCAGTG_nn' Cuts at: 2419 2825 2850 5062 5856 5876 2419 Size: 406 25 2212 794 20 4089 Fragments arranged by size: 4089 2212 794 406 25 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7546 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 4705 4740 529 Size: 35 4141 35 3335 Fragments arranged by size: 4141 3335 35 35 DraI TTT'AAA Cuts at: 2155 2689 4850 2155 Size: 534 2161 4851 Fragments arranged by size: 4851 2161 534 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4613 6553 809 Size: 507 3297 1940 1802 Fragments arranged by size: 3297 1940 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2028 2382 2451 Size: 54 1425 50 275 18 198 354 69 Cuts at: 2451 3087 3237 3456 3786 4993 5882 7511 8 Size: 636 150 219 330 1207 889 1629 43 Fragments arranged by size: 1629 1425 1207 889 636 354 330 275 219 198 150 69 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2028 4993 1537 Size: 275 216 2965 4090 Fragments arranged by size: 4090 2965 275 216 EarI CTCTTCn'nnn_ Cuts at: 1353 2216 2676 3279 3390 3483 3677 5476 1353 Size: 863 460 603 111 93 194 1799 3423 Fragments arranged by size: 3423 1799 863 603 460 194 111 93 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2339 6133 6507 6653 387 Size: 1064 138 750 3794 374 146 1280 Fragments arranged by size: 3794 1280 1064 750 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2738 2891 3089 3170 3278 3317 3464 Size: 393 1106 153 198 81 108 39 147 Cuts at: 3464 3576 3836 3923 4295 5609 6206 6993 7261 Size: 112 260 87 372 1314 597 787 268 Cuts at: 7261 1239 Size: 1524 Fragments arranged by size: 1524 1314 1106 787 597 393 372 268 260 198 153 147 112 108 87 81 39 EcoO109I rG'GnC_Cy Cuts at: 2056 2482 3880 3881 3944 4040 2056 Size: 426 1398 1 63 96 5562 Fragments arranged by size: 5562 1398 426 96 63 1 EcoRI G'AATT_C Cuts at: 4855 5233 4855 Size: 378 7168 EcoRV GAT'ATC Cuts at: 2080 2080 Size: 7546 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2227 2227 Size: 7546 FspI TGC'GCA Cuts at: 3710 4784 6052 3710 Size: 1074 1268 5204 Fragments arranged by size: 5204 1268 1074 HaeII r_GCGC'y Cuts at: 1678 3975 4421 6693 1678 Size: 2297 446 2272 2531 Fragments arranged by size: 2531 2297 2272 446 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2573 2605 3551 3583 3830 Size: 32 135 32 1001 32 946 32 247 Cuts at: 3830 3862 4070 4082 4102 4114 4385 4417 4683 Size: 32 208 12 20 12 271 32 266 Cuts at: 4683 4715 6187 6219 6261 6293 1373 Size: 32 1472 32 42 32 2626 Fragments arranged by size: 2626 1472 1001 946 271 266 247 208 135 42 32 32 32 32 32 32 32 32 32 20 12 12 HincII GTy'rAC Cuts at: 669 1657 1938 4387 4890 5136 669 Size: 988 281 2449 503 246 3079 Fragments arranged by size: 3079 2449 988 503 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7546 HpaI GTT'AAC Cuts at: 5136 5136 Size: 7546 KasI G'GCGC_C Cuts at: 4417 4417 Size: 7546 KpnI G_GTAC'C Cuts at: 3409 4871 3409 Size: 1462 6084 MfeI C'AATT_G Cuts at: 5145 5145 Size: 7546 MluI A'CGCG_T Cuts at: 7422 7422 Size: 7546 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2952 4211 4428 4523 6660 6844 1305 Size: 1647 1259 217 95 2137 184 2007 Fragments arranged by size: 2137 2007 1647 1259 217 184 95 MscI TGG'CCA Cuts at: 10 64 1489 1832 2384 3089 3788 10 Size: 54 1425 343 552 705 699 3768 Fragments arranged by size: 3768 1425 705 699 552 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2318 2402 2625 2982 5504 5863 6022 Size: 648 1152 84 223 357 2522 359 159 Cuts at: 6022 518 Size: 2042 Fragments arranged by size: 2522 2042 1152 648 359 357 223 159 84 MspA1I CmG'CkG Cuts at: 1606 3181 3266 3577 4037 4065 4186 4477 4962 Size: 1575 85 311 460 28 121 291 485 Cuts at: 4962 5639 6787 7032 1606 Size: 677 1148 245 2120 Fragments arranged by size: 2120 1575 1148 677 485 460 311 291 245 121 85 28 NaeI GCC'GGC Cuts at: 2028 2028 Size: 7546 NarI GG'CG_CC Cuts at: 4418 4418 Size: 7546 NcoI C'CATG_G Cuts at: 513 1065 2574 513 Size: 552 1509 5485 Fragments arranged by size: 5485 1509 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7546 NgoMIV G'CCGG_C Cuts at: 2026 2026 Size: 7546 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7546 NotI GC'GGCC_GC Cuts at: 4993 4993 Size: 7546 NspI r_CATG'y Cuts at: 1132 1229 3079 4904 6449 1132 Size: 97 1850 1825 1545 2229 Fragments arranged by size: 2229 1850 1825 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7546 PciI A'CATG_T Cuts at: 6445 6445 Size: 7546 PflMI CCAn_nnn'nTGG Cuts at: 1108 2623 1108 Size: 1515 6031 PfoI T'CCnGG_A Cuts at: 2754 3675 3696 3834 2754 Size: 921 21 138 6466 Fragments arranged by size: 6466 921 138 21 PmeI GTTT'AAAC Cuts at: 4850 4850 Size: 7546 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5615 5647 7128 7160 5615 Size: 32 1481 32 6001 Fragments arranged by size: 6001 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2482 3944 2482 Size: 1462 6084 PshAI GACnn'nnGTC Cuts at: 1613 4204 1613 Size: 2591 4955 PsiI TTA'TAA Cuts at: 5116 5116 Size: 7546 PspOMI G'GGCC_C Cuts at: 3880 4039 3880 Size: 159 7387 PstI C_TGCA'G Cuts at: 830 2641 2820 2845 3036 3750 4332 4898 830 Size: 1811 179 25 191 714 582 566 3478 Fragments arranged by size: 3478 1811 714 582 566 191 179 25 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7546 PvuII CAG'CTG Cuts at: 3266 4186 4477 3266 Size: 920 291 6335 Fragments arranged by size: 6335 920 291 SacII CC_GC'GG Cuts at: 4038 4038 Size: 7546 SalI G'TCGA_C Cuts at: 1936 4888 1936 Size: 2952 4594 SapI GCTCTTCn'nnn_ Cuts at: 2216 3483 2216 Size: 1267 6279 SbfI CC_TGCA'GG Cuts at: 4898 4898 Size: 7546 ScaI AGT'ACT Cuts at: 5794 7506 5794 Size: 1712 5834 SexAI A'CCwGG_T Cuts at: 3338 3504 3338 Size: 166 7380 SfcI C'TryA_G Cuts at: 826 1045 2179 2637 2816 2841 3032 3746 4328 Size: 219 1134 458 179 25 191 714 582 Cuts at: 4328 4894 6029 6710 6901 826 Size: 566 1135 681 191 1471 Fragments arranged by size: 1471 1135 1134 714 681 582 566 458 219 191 191 179 25 SfoI GGC'GCC Cuts at: 4419 4419 Size: 7546 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7546 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7546 SmaI CCC'GGG Cuts at: 1872 4873 1872 Size: 3001 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3866 4202 5650 6551 6813 820 Size: 197 926 1923 336 1448 901 262 1553 Fragments arranged by size: 1923 1553 1448 926 901 336 262 197 SnaBI TAC'GTA Cuts at: 493 4843 5337 493 Size: 4350 494 2702 Fragments arranged by size: 4350 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 7546 SphI G_CATG'C Cuts at: 1132 4904 1132 Size: 3772 3774 SspI AAT'ATT Cuts at: 5 52 5470 5 Size: 47 5418 2081 Fragments arranged by size: 5418 2081 47 StuI AGG'CCT Cuts at: 1517 2459 1517 Size: 942 6604 StyI C'CwwG_G Cuts at: 513 1065 2037 2224 2574 4985 513 Size: 552 972 187 350 2411 3074 Fragments arranged by size: 3074 2411 972 552 350 187 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2615 4614 4733 5573 5590 5743 5928 Size: 1255 1035 1999 119 840 17 153 185 Cuts at: 5928 6373 325 Size: 445 1498 Fragments arranged by size: 1999 1498 1255 1035 840 445 185 153 119 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2547 5792 7504 Size: 274 80 33 51 1443 570 3245 1712 Cuts at: 7504 96 Size: 138 Fragments arranged by size: 3245 1712 1443 570 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3062 3094 7506 7538 3062 Size: 32 4412 32 3070 Fragments arranged by size: 4412 3070 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7546 XbaI T'CTAG_A Cuts at: 4882 4882 Size: 7546 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7546 XmaI C'CCGG_G Cuts at: 1870 4871 1870 Size: 3001 4545 XmnI GAAnn'nnTTC Cuts at: 3671 4583 5675 3671 Size: 912 1092 5542 Fragments arranged by size: 5542 1092 912 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2556 2736 5354 276 Size: 53 83 186 936 1022 180 2618 2468 Fragments arranged by size: 2618 2468 1022 936 186 180 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacII SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbvCI BfrBI BplI BsiWI BstZ17I DraIII EcoICRI EcoNI FseI FspAI NruI NsiI PacI PmlI PspXI PsrI RsrII SacI SanDI SfiI SrfI SwaI XcmI