Restriction map for
pFN21ASDA0873
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# NO CUT ENZYMES ( 138 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AflII AgeI
Alw44I AlwNI AocI Aor51HI ApaI
ApaLI ApoI AscI AseI AsnI
Asp718I AspI AvrII BalI BamHI
BanI BbeI BbrPI BbvCI BcgI
BciVI BfrI BglI BglII BlnI
BlpI Bpu1102I BsaAI BsaHI BseAI
BsgI BsiWI BsmBI Bsp1407I BspDI
BspEI BspHI BsrBI BsrDI BsrFI
BsrGI BssHII Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstXI BstZ17I
Bsu36I CelII Cfr10I ClaI CpoI
DraIII DrdI DsaI Eco47III Eco81I
EcoNI EcoO65I EcoRI EcoT22I FseI
HaeII HgaI Hin1I HincII HindII
HindIII HpaI KasI KpnI KspI
MfeI MluI MluNI MroI MscI
MslI MunI MvnI NaeI NarI
NcoI NdeI NgoMIV NotI NruI
NsiI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacII SalI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI SspBI StuI
SwaI Tsp45I Tth111I Van91I XcmI
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 51 enzymes) #
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AflIII 405 ACATGT A^C[GA][CT]GT
AhdI 222 GACAGAACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Asp700I 226 GAACGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 222 GACAGAACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 1290 TGCGCA TGC^GCA
BanII 912 GAGCTC G[GA]GC[CT]^C
BclI 1025 TGATCA T^GATCA
BcnI 984 CCGGG CC^[GC]GG
BsaI 631 GAGACC GGTCTC(1/5)
BsaWI 269 ACCGGA [AT]^CCGG[AT]
BseRI 1081 GAGGAG GAGGAG(10/8)
BsiEI 699 CGGCCG CG[GA][CT]^CG
BsiYI 977 CCTGCATCCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 977 CCTGCATCCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmFI 306 GTCCC GGGAC(10/14)
BsmI 252 GAATGC GAATGC(1/-1)
BspLU11I 405 ACATGT A^CATGT
BspMI 634 ACCTGC ACCTGC(4/8)
BssSI 310 CTCGTG CACGAG(-5/-1)
BstYI 1133 AGATCC [GA]^GATC[CT]
CfoI 1291 GCGC GCG^C
DraI 828 TTTAAA TTT^AAA
EaeI 699 CGGCCG [CT]^GGCC[GA]
EagI 699 CGGCCG C^GGCCG
Eam1105I 222 GACAGAACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 699 CGGCCG C^GGCCG
Eco52I 699 CGGCCG C^GGCCG
EcoT14I 1116 CCTTGG C^C[AT][AT]GG
FauI 207 GCGGG CCCGC(4/6)
FbaI 1025 TGATCA T^GATCA
FspI 1290 TGCGCA TGC^GCA
HhaI 1291 GCGC GCG^C
HinP1I 1291 GCGC G^CGC
MaeIII 355 GTAAC ^GT[ACGT]AC
MflI 1133 AGATCC [GA]^GATC[CT]
MspA1I 1213 CAGCTG C[AC]G^C[GT]G
NciI 984 CCGGG CC^[GC]GG
NheI 1218 GCTAGC G^CTAGC
NlaIV 593 GGGTCC GG[ACGT]^[ACGT]CC
NspI 405 ACATGT [GA]CATG^[CT]
PleI 160 GACTC GAGTC(4/5)
PstI 636 CTGCAG CTGCA^G
PvuII 1213 CAGCTG CAG^CTG
SacI 912 GAGCTC GAGCT^C
SbfI 635 CCTGCAGG CCTGCA^GG
Sse8387I 635 CCTGCAGG CCTGCA^GG
SspI 1297 AATATT AAT^ATT
StyI 1116 CCTTGG C^C[AT][AT]GG
XbaI 687 TCTAGA T^CTAGA
XhoII 1133 AGATCC [GA]^GATC[CT]
XmnI 226 GAACGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AfaI 906 GTAC GT^AC
1338 GTAC
AlwI 1134 GATCC GGATC(4/5)
1237 GATCC
AvaI 587 CCCGAG C^[CT]CG[GA]G
660 CTCGGG
AvaII 594 GGTCC G^G[AT]CC
843 GGTCC
BbsI 490 GAAGAC GAAGAC(2/6)
230 GTCTTC
BpmI 124 CTGGAG CTGGAG(16/14)
416 CTGGAG
BpuAI 490 GAAGAC GAAGAC(2/6)
230 GTCTTC
BsaBI 532 GATGCACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1159 GATGGAAATC
BsoBI 587 CCCGAG C^[CT]CG[GA]G
660 CTCGGG
EcoRV 385 GATATC GAT^ATC
742 GATATC
HphI 576 GGTGA GGTGA(8/7)
1205 TCACC
MaeII 228 ACGT A^CGT
1112 ACGT
MamI 532 GATGCACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1159 GATGGAAATC
NlaIII 406 CATG CATG^
498 CATG
PpuMI 593 GGGTCCT [GA]G^G[AT]CC[CT]
842 AGGTCCT
RsaI 906 GTAC GT^AC
1338 GTAC
SapI 998 GAAGAGC GCTCTTC(1/4)
1195 GAAGAGC
SfcI 164 CTGTAG C^T[GA][CT]AG
636 CTGCAG
SmlI 847 CTCAAG C^T[CT][GA]AG
1054 CTTGAG
TfiI 132 GATTC G^A[AT]TC
816 GAATC
TspRI 90 CAGTG CA[GC]TG[ACGT][ACGT]^
1004 CAGTG
#########################################
# 3 OR MORE CUT ENZYMES ( 48 enzymes) #
#########################################
AciI AluI AspHI BbvI BfaI
BmyI BsaJI BsiHKAI BsmAI Bsp1286I
BsrI BssKI BstF5I BstNI Cac8I
Cfr13I DdeI DpnII DraII EarI
EcoO109I EcoRII Fnu4HI FokI HaeIII
HapII HinfI HpaII ItaI Ksp632I
MaeI MboI MboII MnlI MseI
MspI MvaI MwoI NdeII Sau3AI
Sau96I ScrFI SfaNI TaqI Tru9I
TseI Tsp509I TthHB8I