(Circular) (Six-base) MAPSORT of: pFN21ASDA0873.seq Check: 3981 from: 1 to: 6070 pFN21ASDA0873 6070 bp With 182 enzymes: SgfI * January 16, 2009 15:30 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3880 278 Size: 53 83 186 936 2344 2468 Fragments arranged by size: 2468 2344 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3413 1937 Size: 1476 4594 Acc65I G'GTAC_C Cuts at: 3391 3391 Size: 6070 AclI AA'CG_TT Cuts at: 1563 4197 4570 1563 Size: 2634 373 3063 Fragments arranged by size: 3063 2634 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2045 2095 2217 4133 5517 5785 1632 Size: 413 50 122 1916 1384 268 1917 Fragments arranged by size: 1917 1916 1384 413 268 122 50 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5873 AflIII A'CryG_T Cuts at: 1203 2428 4969 5946 1203 Size: 1225 2541 977 1327 Fragments arranged by size: 2541 1327 1225 977 AgeI A'CCGG_T Cuts at: 4901 4901 Size: 6070 AhdI GACnn_n'nnGTC Cuts at: 2250 4799 2250 Size: 2549 3521 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6070 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6070 AlwNI CAG_nnn'CTG Cuts at: 3813 5385 3813 Size: 1572 4498 ApaLI G'TGCA_C Cuts at: 4127 5283 4127 Size: 1156 4914 ApoI r'AATT_y Cuts at: 1493 3379 3605 3757 5866 1493 Size: 1886 226 152 2109 1697 Fragments arranged by size: 2109 1886 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4624 160 Size: 872 3592 1606 Fragments arranged by size: 3592 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6070 AvaI C'yCGr_G Cuts at: 1870 1943 2610 2683 3395 1870 Size: 73 667 73 712 4545 Fragments arranged by size: 4545 712 667 73 73 BamHI G'GATC_C Cuts at: 3400 3767 3400 Size: 367 5703 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3391 4846 618 Size: 325 217 627 1604 1455 1842 Fragments arranged by size: 1842 1604 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2939 3389 1391 Size: 1548 450 4072 Fragments arranged by size: 4072 1548 450 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2246 2520 928 Size: 971 347 274 4478 Fragments arranged by size: 4478 971 347 274 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4261 4295 1903 Size: 34 2324 34 3678 Fragments arranged by size: 3678 2324 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3962 5172 3962 Size: 1210 4860 BclI T'GATC_A Cuts at: 1548 3048 1548 Size: 1500 4570 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4681 136 Size: 107 122 71 631 605 3009 1525 Fragments arranged by size: 3009 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6065 6065 Size: 6070 BlpI GC'TnA_GC Cuts at: 3487 3487 Size: 6070 Bme1580I G_kGCm'C Cuts at: 1790 4131 5287 1790 Size: 2341 1156 2573 Fragments arranged by size: 2573 2341 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6070 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2320 3801 4759 Size: 416 53 83 399 24 903 1481 958 Cuts at: 4759 442 Size: 1753 Fragments arranged by size: 1753 1481 958 903 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 3245 1056 Size: 2189 3881 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2168 2460 4730 1239 Size: 929 292 2270 2579 Fragments arranged by size: 2579 2270 929 292 Bpu10I CC'TnA_GC Cuts at: 2933 2933 Size: 6070 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2855 3098 4195 5060 5358 2855 Size: 243 1097 865 298 3567 Fragments arranged by size: 3567 1097 865 298 243 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2648 4733 882 Size: 617 1149 2085 2219 Fragments arranged by size: 2219 2085 1149 617 BsaAI yAC'GTr Cuts at: 493 1204 3367 3861 493 Size: 711 2163 494 2702 Fragments arranged by size: 2702 2163 711 494 BsaBI GATnn'nnATC Cuts at: 2559 3186 6064 2559 Size: 627 2878 2565 Fragments arranged by size: 2878 2565 627 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3877 4259 4894 275 Size: 53 83 186 936 2344 382 635 1451 Fragments arranged by size: 2344 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2292 4503 4901 5175 5322 5733 2292 Size: 2211 398 274 147 411 2629 Fragments arranged by size: 2629 2211 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2921 2951 1178 Size: 30 165 30 1518 30 4297 Fragments arranged by size: 4297 1518 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3119 1186 Size: 1933 4137 BseYI C'CCAG_C Cuts at: 5273 5769 5273 Size: 496 5574 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5497 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2725 3520 4281 5309 1540 Size: 275 186 724 795 761 1028 2301 Fragments arranged by size: 2301 1028 795 761 724 275 186 BsiHKAI G_wGCw'C Cuts at: 2682 2939 3111 4131 4216 5287 2682 Size: 257 172 1020 85 1071 3465 Fragments arranged by size: 3465 1071 1020 257 172 85 BsmI GAATG_Cn' Cuts at: 2281 3581 3674 2281 Size: 1300 93 4677 Fragments arranged by size: 4677 1300 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2682 2939 3111 3389 4131 4216 5287 Size: 399 892 257 172 278 742 85 1071 Cuts at: 5287 1391 Size: 2174 Fragments arranged by size: 2174 1071 892 742 399 278 257 172 85 BspEI T'CCGG_A Cuts at: 5733 5733 Size: 6070 BspHI T'CATG_A Cuts at: 3959 5689 3959 Size: 1730 4340 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2666 3425 844 Size: 1822 759 3489 Fragments arranged by size: 3489 1822 759 BsrBI CCG'CTC Cuts at: 1394 1823 3957 1394 Size: 429 2134 3507 Fragments arranged by size: 3507 2134 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4565 4739 65 Size: 1183 3317 174 1396 Fragments arranged by size: 3317 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4714 4901 1700 Size: 110 2904 187 2869 Fragments arranged by size: 2904 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6070 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6070 BssSI C'ACGA_G Cuts at: 2333 4130 5142 2333 Size: 1797 1012 3261 Fragments arranged by size: 3261 1797 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6070 BstBI TT'CG_AA Cuts at: 2007 4874 2007 Size: 2867 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6070 BstXI CCAn_nnnn'nTGG Cuts at: 2029 2029 Size: 6070 BstYI r'GATC_y Cuts at: 1972 3156 3400 3767 4152 4169 5621 5707 5719 Size: 1184 244 367 385 17 1452 86 12 Cuts at: 5719 6065 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1184 385 367 346 244 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2022 513 Size: 552 957 4561 Fragments arranged by size: 4561 957 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5331 Fragments arranged by size: 5331 683 56 BtsI GCAGTG_nn' Cuts at: 3033 3586 4380 4400 3033 Size: 553 794 20 4703 Fragments arranged by size: 4703 794 553 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6070 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6035 DraI TTT'AAA Cuts at: 2853 3374 2853 Size: 521 5549 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5077 809 Size: 507 3761 1802 Fragments arranged by size: 3761 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2722 3517 4406 Size: 54 1425 50 275 18 892 795 889 Cuts at: 4406 6035 8 Size: 1629 43 Fragments arranged by size: 1629 1425 892 889 795 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2722 3517 1537 Size: 275 910 795 4090 Fragments arranged by size: 4090 910 795 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2793 3016 3213 3267 4000 1353 Size: 1440 223 197 54 733 3423 Fragments arranged by size: 3423 1440 733 223 197 54 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4657 5031 5177 387 Size: 1064 138 3068 374 146 1280 Fragments arranged by size: 3068 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2937 2937 Size: 6070 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2045 2095 2168 2217 2460 4133 4730 Size: 393 413 50 73 49 243 1673 597 Cuts at: 4730 5517 5785 1239 Size: 787 268 1524 Fragments arranged by size: 1673 1524 787 597 413 393 268 243 73 50 49 EcoO109I rG'GnC_Cy Cuts at: 2617 2866 3073 2617 Size: 249 207 5614 Fragments arranged by size: 5614 249 207 EcoRI G'AATT_C Cuts at: 3379 3757 3379 Size: 378 5692 EcoRV GAT'ATC Cuts at: 2410 2767 2410 Size: 357 5713 FspI TGC'GCA Cuts at: 3315 4576 3315 Size: 1261 4809 HaeII r_GCGC'y Cuts at: 1678 5217 1678 Size: 3539 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2236 2268 2433 2465 2824 Size: 32 135 32 664 32 165 32 359 Cuts at: 2824 2856 3015 3047 3098 3130 3329 3361 4711 Size: 32 159 32 51 32 199 32 1350 Cuts at: 4711 4743 4785 4817 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1350 664 359 199 165 159 135 51 42 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3414 3660 669 Size: 988 281 1476 246 3079 Fragments arranged by size: 3079 1476 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6070 HpaI GTT'AAC Cuts at: 3660 3660 Size: 6070 KpnI G_GTAC'C Cuts at: 3395 3395 Size: 6070 MfeI C'AATT_G Cuts at: 3669 3669 Size: 6070 MluI A'CGCG_T Cuts at: 5946 5946 Size: 6070 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2247 2952 5184 5368 1305 Size: 942 705 2232 184 2007 Fragments arranged by size: 2232 2007 942 705 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4248 Fragments arranged by size: 4248 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2027 4028 4387 4546 518 Size: 648 861 2001 359 159 2042 Fragments arranged by size: 2042 2001 861 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3238 3486 4163 5311 5556 1606 Size: 1632 248 677 1148 245 2120 Fragments arranged by size: 2120 1632 1148 677 248 245 NcoI C'CATG_G Cuts at: 513 1065 2022 513 Size: 552 957 4561 Fragments arranged by size: 4561 957 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6070 NheI G'CTAG_C Cuts at: 1052 3241 1052 Size: 2189 3881 NotI GC'GGCC_GC Cuts at: 3517 3517 Size: 6070 NspI r_CATG'y Cuts at: 1132 1229 2432 3428 4973 1132 Size: 97 1203 996 1545 2229 Fragments arranged by size: 2229 1545 1203 996 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6070 PciI A'CATG_T Cuts at: 2428 4969 2428 Size: 2541 3529 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6070 PfoI T'CCnGG_A Cuts at: 3006 3006 Size: 6070 PmeI GTTT'AAAC Cuts at: 3374 3374 Size: 6070 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2241 2273 4139 4171 5652 5684 2241 Size: 32 1866 32 1481 32 2627 Fragments arranged by size: 2627 1866 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2617 2866 2617 Size: 249 5821 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6070 PsiI TTA'TAA Cuts at: 2361 3640 2361 Size: 1279 4791 PstI C_TGCA'G Cuts at: 830 2663 3422 830 Size: 1833 759 3478 Fragments arranged by size: 3478 1833 759 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6070 PvuII CAG'CTG Cuts at: 3238 3238 Size: 6070 SacI G_AGCT'C Cuts at: 2939 2939 Size: 6070 SalI G'TCGA_C Cuts at: 1936 3412 1936 Size: 1476 4594 SapI GCTCTTCn'nnn_ Cuts at: 3016 3213 3016 Size: 197 5873 SbfI CC_TGCA'GG Cuts at: 2663 3422 2663 Size: 759 5311 ScaI AGT'ACT Cuts at: 3362 4318 6030 3362 Size: 956 1712 3402 Fragments arranged by size: 3402 1712 956 SfcI C'TryA_G Cuts at: 826 1045 2187 2659 3418 4553 5234 5425 826 Size: 219 1142 472 759 1135 681 191 1471 Fragments arranged by size: 1471 1142 1135 759 681 472 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6070 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6070 SmaI CCC'GGG Cuts at: 1872 3397 1872 Size: 1525 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2870 3077 4174 5075 5337 820 Size: 197 926 927 207 1097 901 262 1553 Fragments arranged by size: 1553 1097 927 926 901 262 207 197 SnaBI TAC'GTA Cuts at: 493 3367 3861 493 Size: 2874 494 2702 Fragments arranged by size: 2874 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 6070 SphI G_CATG'C Cuts at: 1132 3428 1132 Size: 2296 3774 SspI AAT'ATT Cuts at: 5 52 3322 3994 5 Size: 47 3270 672 2081 Fragments arranged by size: 3270 2081 672 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6070 StyI C'CwwG_G Cuts at: 513 1065 2022 3139 3509 513 Size: 552 957 1117 370 3074 Fragments arranged by size: 3074 1117 957 552 370 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2301 2627 3126 4097 4114 4267 4452 Size: 1255 721 326 499 971 17 153 185 Cuts at: 4452 4897 325 Size: 445 1498 Fragments arranged by size: 1498 1255 971 721 499 445 326 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3360 4316 6028 Size: 274 80 33 51 1443 1383 956 1712 Cuts at: 6028 96 Size: 138 Fragments arranged by size: 1712 1443 1383 956 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6030 6062 6030 Size: 32 6038 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6070 XbaI T'CTAG_A Cuts at: 2710 3406 2710 Size: 696 5374 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6070 XmaI C'CCGG_G Cuts at: 1870 3395 1870 Size: 1525 4545 XmnI GAAnn'nnTTC Cuts at: 2253 4199 2253 Size: 1946 4124 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3878 276 Size: 53 83 186 936 2344 2468 Fragments arranged by size: 2468 2344 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI