Restriction map for
pFN21ASDA0873
(Linear) (Six-base) MAPSORT of: KIAA0873.seq Check: 9770 from: 1 to: 1341
KIAA0873.orf
With 182 enzymes: SgfI *
October 24, 2006 15:14 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 22 72 194 1341
Size: 22 50 122 1147
Fragments arranged by size:
1147 122 50 22
AflIII A'CryG_T
Cuts at: 0 405 1341
Size: 405 936
AhdI GACnn_n'nnGTC
Cuts at: 0 227 1341
Size: 227 1114
AvaI C'yCGr_G
Cuts at: 0 587 660 1341
Size: 587 73 681
Fragments arranged by size:
681 587 73
BanII G_rGCy'C
Cuts at: 0 916 1341
Size: 916 425
BbsI GAAGACnn'nnnn_
Cuts at: 0 223 497 1341
Size: 223 274 844
Fragments arranged by size:
844 274 223
BclI T'GATC_A
Cuts at: 0 1025 1341
Size: 1025 316
BmrI ACTGGGnnnn_n'
Cuts at: 0 297 1341
Size: 297 1044
BmtI G_CTAG'C
Cuts at: 0 1222 1341
Size: 1222 119
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 145 437 1341
Size: 145 292 904
Fragments arranged by size:
904 292 145
Bpu10I CC'TnA_GC
Cuts at: 0 910 1341
Size: 910 431
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 832 1075 1341
Size: 832 243 266
Fragments arranged by size:
832 266 243
BsaI GGTCTCn'nnnn_
Cuts at: 0 625 1341
Size: 625 716
BsaBI GATnn'nnATC
Cuts at: 0 536 1163 1341
Size: 536 627 178
Fragments arranged by size:
627 536 178
BsaWI w'CCGG_w
Cuts at: 0 269 1341
Size: 269 1072
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 898 928 1341
Size: 898 30 413
Fragments arranged by size:
898 413 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1096 1341
Size: 1096 245
BsiEI CG_ry'CG
Cuts at: 0 702 1341
Size: 702 639
BsiHKAI G_wGCw'C
Cuts at: 0 659 916 1088 1341
Size: 659 257 172 253
Fragments arranged by size:
659 257 253 172
BsmI GAATG_Cn'
Cuts at: 0 258 1341
Size: 258 1083
Bsp1286I G_dGCh'C
Cuts at: 0 659 916 1088 1341
Size: 659 257 172 253
Fragments arranged by size:
659 257 253 172
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 643 1341
Size: 643 698
BssSI C'ACGA_G
Cuts at: 0 310 1341
Size: 310 1031
BstYI r'GATC_y
Cuts at: 0 1133 1341
Size: 1133 208
BtsI GCAGTG_nn'
Cuts at: 0 1010 1341
Size: 1010 331
DraI TTT'AAA
Cuts at: 0 830 1341
Size: 830 511
EaeI y'GGCC_r
Cuts at: 0 699 1341
Size: 699 642
EagI C'GGCC_G
Cuts at: 0 699 1341
Size: 699 642
EarI CTCTTCn'nnn_
Cuts at: 0 770 993 1190 1244 1341
Size: 770 223 197 54 97
Fragments arranged by size:
770 223 197 97 54
EcoICRI GAG'CTC
Cuts at: 0 914 1341
Size: 914 427
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 22 72 145 194 437 1341
Size: 22 50 73 49 243 904
Fragments arranged by size:
904 243 73 50 49 22
EcoO109I rG'GnC_Cy
Cuts at: 0 594 843 1050 1341
Size: 594 249 207 291
Fragments arranged by size:
594 291 249 207
EcoRV GAT'ATC
Cuts at: 0 387 744 1341
Size: 387 357 597
Fragments arranged by size:
597 387 357
FspI TGC'GCA
Cuts at: 0 1292 1341
Size: 1292 49
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 213 245 410 442 801 833 992 1024
Size: 213 32 165 32 359 32 159 32
Cuts at: 1024 1075 1107 1306 1338 1341
Size: 51 32 199 32 3
Fragments arranged by size:
359 213 199 165 159 51 32 32
32 32 32 32 3
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 224 929 1341
Size: 224 705 412
Fragments arranged by size:
705 412 224
MspA1I CmG'CkG
Cuts at: 0 1215 1341
Size: 1215 126
NheI G'CTAG_C
Cuts at: 0 1218 1341
Size: 1218 123
NspI r_CATG'y
Cuts at: 0 409 1341
Size: 409 932
PciI A'CATG_T
Cuts at: 0 405 1341
Size: 405 936
PfoI T'CCnGG_A
Cuts at: 0 983 1341
Size: 983 358
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 218 250 1341
Size: 218 32 1091
Fragments arranged by size:
1091 218 32
PpuMI rG'GwC_Cy
Cuts at: 0 594 843 1341
Size: 594 249 498
Fragments arranged by size:
594 498 249
PsiI TTA'TAA
Cuts at: 0 338 1341
Size: 338 1003
PstI C_TGCA'G
Cuts at: 0 640 1341
Size: 640 701
PvuII CAG'CTG
Cuts at: 0 1215 1341
Size: 1215 126
SacI G_AGCT'C
Cuts at: 0 916 1341
Size: 916 425
SapI GCTCTTCn'nnn_
Cuts at: 0 993 1190 1341
Size: 993 197 151
Fragments arranged by size:
993 197 151
SbfI CC_TGCA'GG
Cuts at: 0 640 1341
Size: 640 701
SfcI C'TryA_G
Cuts at: 0 164 636 1341
Size: 164 472 705
Fragments arranged by size:
705 472 164
SmlI C'TyrA_G
Cuts at: 0 847 1054 1341
Size: 847 207 287
Fragments arranged by size:
847 287 207
SspI AAT'ATT
Cuts at: 0 1299 1341
Size: 1299 42
StyI C'CwwG_G
Cuts at: 0 1116 1341
Size: 1116 225
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 278 604 1103 1341
Size: 278 326 499 238
Fragments arranged by size:
499 326 278 238
XbaI T'CTAG_A
Cuts at: 0 687 1341
Size: 687 654
XmnI GAAnn'nnTTC
Cuts at: 0 230 1341
Size: 230 1111
Enzymes that do cut:
AcuI AflIII AhdI AvaI BanII BbsI BclI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaBI BsaWI BsaXI
BseRI BsiEI BsiHKAI BsmI Bsp1286I BspMI BssSI BstYI
BtsI DraI EaeI EagI EarI EcoICRI Eco57MI EcoO109I
EcoRV FspI Hin4I MmeI MspA1I NheI NspI PciI
PfoI PpiI PpuMI PsiI PstI PvuII SacI SapI
SbfI SfcI SmlI SspI StyI TaqII XbaI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AleI AlfI AloI AlwNI ApaI ApaLI ApoI AscI
AseI AsiSI AvrII BaeI BamHI BanI BbeI BbvCI
BcgI BciVI BfrBI BglI BglII BlpI Bme1580I BmgBI
BplI BsaAI BsaHI BseYI BsgI BsiWI BsmBI BspEI
BspHI BsrBI BsrDI BsrFI BsrGI BssHII BstAPI BstBI
BstEII BstXI BstZ17I Bsu36I BtgI BtgZI ClaI CspCI
DraIII DrdI EciI EcoNI EcoRI FalI FseI FspAI
HaeII HincII HindIII HpaI KasI KpnI MfeI MluI
MscI MslI NaeI NarI NcoI NdeI NgoMIV NotI
NruI NsiI PacI PasI PflMI PmeI PmlI PshAI
PspOMI PspXI PsrI PvuI RsrII SacII SalI SanDI
ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI
SpeI SphI SrfI StuI SwaI TatI TstI Tth111I
XcmI XhoI XmaI ZraI