(Circular) (Six-base) MAPSORT of: pFN21ASDA0871.seq Check: 4720 from: 1 to: 6136 pFN21ASDA0871 6136 bp With 182 enzymes: SgfI * January 16, 2009 15:30 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3946 278 Size: 53 83 186 936 2410 2468 Fragments arranged by size: 2468 2410 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3479 1937 Size: 1542 4594 Acc65I G'GTAC_C Cuts at: 3457 3457 Size: 6136 AclI AA'CG_TT Cuts at: 1563 4263 4636 1563 Size: 2700 373 3063 Fragments arranged by size: 3063 2700 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2796 2849 4199 5583 5851 1632 Size: 1164 53 1350 1384 268 1917 Fragments arranged by size: 1917 1384 1350 1164 268 53 AflII C'TTAA_G Cuts at: 820 1017 2489 820 Size: 197 1472 4467 Fragments arranged by size: 4467 1472 197 AflIII A'CryG_T Cuts at: 1203 5035 6012 1203 Size: 3832 977 1327 Fragments arranged by size: 3832 1327 977 AgeI A'CCGG_T Cuts at: 4967 4967 Size: 6136 AhdI GACnn_n'nnGTC Cuts at: 4865 4865 Size: 6136 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6136 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3261 1184 Size: 2077 4059 AlwNI CAG_nnn'CTG Cuts at: 3214 3879 5451 3214 Size: 665 1572 3899 Fragments arranged by size: 3899 1572 665 ApaLI G'TGCA_C Cuts at: 4193 5349 4193 Size: 1156 4980 ApoI r'AATT_y Cuts at: 1493 2111 2597 3445 3671 3823 5932 1493 Size: 618 486 848 226 152 2109 1697 Fragments arranged by size: 2109 1697 848 618 486 226 152 AseI AT'TA_AT Cuts at: 160 1032 2534 4690 160 Size: 872 1502 2156 1606 Fragments arranged by size: 2156 1606 1502 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6136 AvaI C'yCGr_G Cuts at: 1870 1943 3461 1870 Size: 73 1518 4545 Fragments arranged by size: 4545 1518 73 BamHI G'GATC_C Cuts at: 3466 3833 3466 Size: 367 5769 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3457 4912 618 Size: 325 217 627 1670 1455 1842 Fragments arranged by size: 1842 1670 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 3232 3455 1391 Size: 1841 223 4072 Fragments arranged by size: 4072 1841 223 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2709 3323 928 Size: 971 810 614 3741 Fragments arranged by size: 3741 971 810 614 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2032 2066 2594 2628 4327 4361 1903 Size: 34 95 34 528 34 1699 34 3678 Fragments arranged by size: 3678 1699 528 95 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4028 5238 4028 Size: 1210 4926 BclI T'GATC_A Cuts at: 1548 2571 1548 Size: 1023 5113 BfrBI ATG'CAT Cuts at: 2882 2882 Size: 6136 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4747 136 Size: 107 122 71 631 605 3075 1525 Fragments arranged by size: 3075 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2476 6131 2476 Size: 3655 2481 BlpI GC'TnA_GC Cuts at: 3553 3553 Size: 6136 Bme1580I G_kGCm'C Cuts at: 1790 4197 5353 1790 Size: 2407 1156 2573 Fragments arranged by size: 2573 2407 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6136 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3867 4825 442 Size: 416 53 83 399 24 2450 958 1753 Fragments arranged by size: 2450 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6136 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2156 2156 Size: 6136 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2309 3131 3182 4796 1239 Size: 1070 822 51 1614 2579 Fragments arranged by size: 2579 1614 1070 822 51 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2732 3218 4261 5126 5424 2732 Size: 486 1043 865 298 3444 Fragments arranged by size: 3444 1043 865 486 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2087 4799 882 Size: 617 588 2712 2219 Fragments arranged by size: 2712 2219 617 588 BsaAI yAC'GTr Cuts at: 493 1204 3433 3927 493 Size: 711 2229 494 2702 Fragments arranged by size: 2702 2229 711 494 BsaBI GATnn'nnATC Cuts at: 3119 6130 3119 Size: 3011 3125 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2036 3943 4325 4960 Size: 53 83 186 936 503 1907 382 635 Cuts at: 4960 275 Size: 1451 Fragments arranged by size: 1907 1451 936 635 503 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4569 4967 5241 5388 5799 4569 Size: 398 274 147 411 4906 Fragments arranged by size: 4906 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5911 Fragments arranged by size: 5911 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2927 1186 Size: 1741 4395 BseYI C'CCAG_C Cuts at: 5339 5835 5339 Size: 496 5640 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3420 1163 Size: 573 1684 3879 Fragments arranged by size: 3879 1684 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2046 3586 4347 5375 1540 Size: 275 186 45 1540 761 1028 2301 Fragments arranged by size: 2301 1540 1028 761 275 186 45 BsiHKAI G_wGCw'C Cuts at: 2352 3232 4197 4282 5353 2352 Size: 880 965 85 1071 3135 Fragments arranged by size: 3135 1071 965 880 85 BsmI GAATG_Cn' Cuts at: 2851 3647 3740 2851 Size: 796 93 5247 Fragments arranged by size: 5247 796 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2775 2775 Size: 6136 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2352 3232 3455 4197 4282 5353 1391 Size: 399 562 880 223 742 85 1071 2174 Fragments arranged by size: 2174 1071 880 742 562 399 223 85 BspEI T'CCGG_A Cuts at: 5799 5799 Size: 6136 BspHI T'CATG_A Cuts at: 2238 2619 4025 5755 2238 Size: 381 1406 1730 2619 Fragments arranged by size: 2619 1730 1406 381 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3491 844 Size: 2647 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4023 1394 Size: 429 2200 3507 Fragments arranged by size: 3507 2200 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4631 4805 65 Size: 1183 3383 174 1396 Fragments arranged by size: 3383 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4780 4967 1700 Size: 110 2970 187 2869 Fragments arranged by size: 2970 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6136 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6136 BssSI C'ACGA_G Cuts at: 4196 5208 4196 Size: 1012 5124 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6136 BstBI TT'CG_AA Cuts at: 2007 4940 2007 Size: 2933 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6136 BstYI r'GATC_y Cuts at: 1972 2200 2476 3466 3833 4218 4235 5687 5773 Size: 228 276 990 367 385 17 1452 86 Cuts at: 5773 5785 6131 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 990 385 367 346 276 228 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2088 2124 513 Size: 552 1023 36 4525 Fragments arranged by size: 4525 1023 552 36 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5397 Fragments arranged by size: 5397 683 56 BtsI GCAGTG_nn' Cuts at: 3652 4446 4466 3652 Size: 794 20 5322 Fragments arranged by size: 5322 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6136 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6101 DraI TTT'AAA Cuts at: 3069 3440 3069 Size: 371 5765 DraIII CAC_nnn'GTG Cuts at: 3050 3050 Size: 6136 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5143 809 Size: 507 3827 1802 Fragments arranged by size: 3827 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2247 3583 4472 Size: 54 1425 50 275 18 417 1336 889 Cuts at: 4472 6101 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1336 889 417 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3583 1537 Size: 275 1771 4090 Fragments arranged by size: 4090 1771 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2506 3069 3207 3306 4066 1353 Size: 1153 563 138 99 760 3423 Fragments arranged by size: 3423 1153 760 563 138 99 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4723 5097 5243 387 Size: 1064 138 3134 374 146 1280 Fragments arranged by size: 3134 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3230 3230 Size: 6136 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2309 2796 2849 3131 3182 4199 4796 Size: 393 677 487 53 282 51 1017 597 Cuts at: 4796 5583 5851 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1017 787 677 597 487 393 282 268 53 51 EcoO109I rG'GnC_Cy Cuts at: 2414 3011 2414 Size: 597 5539 EcoRI G'AATT_C Cuts at: 2597 3445 3823 2597 Size: 848 378 4910 Fragments arranged by size: 4910 848 378 FspI TGC'GCA Cuts at: 4642 4642 Size: 6136 HaeII r_GCGC'y Cuts at: 1678 5283 1678 Size: 3605 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2150 2182 2696 2728 4777 Size: 32 135 32 578 32 514 32 2049 Cuts at: 4777 4809 4851 4883 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2049 578 514 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3480 3726 669 Size: 988 281 1542 246 3079 Fragments arranged by size: 3079 1542 988 281 246 HindIII A'AGCT_T Cuts at: 748 2435 2565 748 Size: 1687 130 4319 Fragments arranged by size: 4319 1687 130 HpaI GTT'AAC Cuts at: 3726 3726 Size: 6136 KpnI G_GTAC'C Cuts at: 3461 3461 Size: 6136 MfeI C'AATT_G Cuts at: 3735 3735 Size: 6136 MluI A'CGCG_T Cuts at: 6012 6012 Size: 6136 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2066 2701 2919 5250 5434 1305 Size: 761 635 218 2331 184 2007 Fragments arranged by size: 2331 2007 761 635 218 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2249 10 Size: 54 1425 343 417 3897 Fragments arranged by size: 3897 1425 417 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2243 2844 4094 4453 4612 518 Size: 648 1077 601 1250 359 159 2042 Fragments arranged by size: 2042 1250 1077 648 601 359 159 MspA1I CmG'CkG Cuts at: 1606 3214 3552 4229 5377 5622 1606 Size: 1608 338 677 1148 245 2120 Fragments arranged by size: 2120 1608 1148 677 338 245 NcoI C'CATG_G Cuts at: 513 1065 2088 2124 513 Size: 552 1023 36 4525 Fragments arranged by size: 4525 1023 552 36 NdeI CA'TA_TG Cuts at: 387 387 Size: 6136 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6136 NotI GC'GGCC_GC Cuts at: 3583 3583 Size: 6136 NsiI A_TGCA'T Cuts at: 2884 2884 Size: 6136 NspI r_CATG'y Cuts at: 1132 1229 3494 5039 1132 Size: 97 2265 1545 2229 Fragments arranged by size: 2265 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6136 PciI A'CATG_T Cuts at: 5035 5035 Size: 6136 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6136 PfoI T'CCnGG_A Cuts at: 2482 2482 Size: 6136 PmeI GTTT'AAAC Cuts at: 3440 3440 Size: 6136 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4205 4237 5718 5750 4205 Size: 32 1481 32 4591 Fragments arranged by size: 4591 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3011 3011 Size: 6136 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6136 PsiI TTA'TAA Cuts at: 3706 3706 Size: 6136 PstI C_TGCA'G Cuts at: 830 3034 3261 3488 830 Size: 2204 227 227 3478 Fragments arranged by size: 3478 2204 227 227 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6136 PvuII CAG'CTG Cuts at: 3214 3214 Size: 6136 SacI G_AGCT'C Cuts at: 3232 3232 Size: 6136 SalI G'TCGA_C Cuts at: 1936 3478 1936 Size: 1542 4594 SanDI GG'GwC_CC Cuts at: 3011 3011 Size: 6136 SapI GCTCTTCn'nnn_ Cuts at: 2506 2506 Size: 6136 SbfI CC_TGCA'GG Cuts at: 3488 3488 Size: 6136 ScaI AGT'ACT Cuts at: 4384 6096 4384 Size: 1712 4424 SfcI C'TryA_G Cuts at: 826 1045 3030 3257 3484 4619 5300 5491 826 Size: 219 1985 227 227 1135 681 191 1471 Fragments arranged by size: 1985 1471 1135 681 227 227 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6136 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6136 SmaI CCC'GGG Cuts at: 1872 3463 1872 Size: 1591 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2489 2747 3233 4240 5141 5403 Size: 197 926 546 258 486 1007 901 262 Cuts at: 5403 820 Size: 1553 Fragments arranged by size: 1553 1007 926 901 546 486 262 258 197 SnaBI TAC'GTA Cuts at: 493 3433 3927 493 Size: 2940 494 2702 Fragments arranged by size: 2940 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 6136 SphI G_CATG'C Cuts at: 1132 3494 1132 Size: 2362 3774 SspI AAT'ATT Cuts at: 5 52 4060 5 Size: 47 4008 2081 Fragments arranged by size: 4008 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6136 StyI C'CwwG_G Cuts at: 513 1065 2088 2124 3575 513 Size: 552 1023 36 1451 3074 Fragments arranged by size: 3074 1451 1023 552 36 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2060 4163 4180 4333 4518 4963 325 Size: 1255 480 2103 17 153 185 445 1498 Fragments arranged by size: 2103 1498 1255 480 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4382 6094 96 Size: 274 80 33 51 1443 2405 1712 138 Fragments arranged by size: 2405 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6096 6128 6096 Size: 32 6104 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6136 XbaI T'CTAG_A Cuts at: 2419 3472 2419 Size: 1053 5083 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6136 XmaI C'CCGG_G Cuts at: 1870 3461 1870 Size: 1591 4545 XmnI GAAnn'nnTTC Cuts at: 4265 4265 Size: 6136 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3944 276 Size: 53 83 186 936 2410 2468 Fragments arranged by size: 2468 2410 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SanDI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI Bpu10I BsiWI BstXI BstZ17I Bsu36I EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PspOMI PspXI PsrI RsrII SacII SexAI SfiI SfoI SrfI SwaI XcmI