(Circular) (Six-base) MAPSORT of: pFN21ASDA0862.seq Check: 1862 from: 1 to: 6475 pFN21ASDA0862 6475 bp With 182 enzymes: SgfI * January 16, 2009 15:30 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4285 278 Size: 53 83 186 936 2749 2468 Fragments arranged by size: 2749 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3768 3818 1937 Size: 1831 50 4594 Fragments arranged by size: 4594 1831 50 Acc65I G'GTAC_C Cuts at: 3796 3796 Size: 6475 AclI AA'CG_TT Cuts at: 1563 4602 4975 1563 Size: 3039 373 3063 Fragments arranged by size: 3063 3039 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2562 3573 4538 5922 6190 1632 Size: 930 1011 965 1384 268 1917 Fragments arranged by size: 1917 1384 1011 965 930 268 AflII C'TTAA_G Cuts at: 820 1017 3431 820 Size: 197 2414 3864 Fragments arranged by size: 3864 2414 197 AflIII A'CryG_T Cuts at: 1203 5374 6351 1203 Size: 4171 977 1327 Fragments arranged by size: 4171 1327 977 AgeI A'CCGG_T Cuts at: 5306 5306 Size: 6475 AhdI GACnn_n'nnGTC Cuts at: 5204 5204 Size: 6475 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6475 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6475 AlwNI CAG_nnn'CTG Cuts at: 2258 4218 5790 2258 Size: 1960 1572 2943 Fragments arranged by size: 2943 1960 1572 ApaLI G'TGCA_C Cuts at: 2943 4532 5688 2943 Size: 1589 1156 3730 Fragments arranged by size: 3730 1589 1156 ApoI r'AATT_y Cuts at: 1493 2323 2488 2571 2959 3129 3141 3244 3784 Size: 830 165 83 388 170 12 103 540 Cuts at: 3784 4010 4162 6271 1493 Size: 226 152 2109 1697 Fragments arranged by size: 2109 1697 830 540 388 226 170 165 152 103 83 12 AseI AT'TA_AT Cuts at: 160 1032 5029 160 Size: 872 3997 1606 Fragments arranged by size: 3997 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6475 AvaI C'yCGr_G Cuts at: 1870 1943 3800 1870 Size: 73 1857 4545 Fragments arranged by size: 4545 1857 73 BamHI G'GATC_C Cuts at: 3805 4172 3805 Size: 367 6108 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3796 5251 618 Size: 325 217 627 2009 1455 1842 Fragments arranged by size: 2009 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2304 3794 1391 Size: 913 1490 4072 Fragments arranged by size: 4072 1490 913 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5504 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4666 4700 1903 Size: 34 2729 34 3678 Fragments arranged by size: 3678 2729 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3069 4367 5577 3069 Size: 1298 1210 3967 Fragments arranged by size: 3967 1298 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 6475 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5086 136 Size: 107 122 71 631 605 3414 1525 Fragments arranged by size: 3414 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2351 6470 2351 Size: 4119 2356 BlpI GC'TnA_GC Cuts at: 2681 3892 2681 Size: 1211 5264 Bme1580I G_kGCm'C Cuts at: 1790 2947 4536 5692 1790 Size: 1157 1589 1156 2573 Fragments arranged by size: 2573 1589 1157 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6475 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4206 5164 442 Size: 416 53 83 399 24 2789 958 1753 Fragments arranged by size: 2789 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6475 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2136 2176 3133 3590 5135 1239 Size: 897 40 957 457 1545 2579 Fragments arranged by size: 2579 1545 957 897 457 40 Bpu10I CC'TnA_GC Cuts at: 3034 3034 Size: 6475 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3323 3647 4600 5465 5763 3323 Size: 324 953 865 298 4035 Fragments arranged by size: 4035 953 865 324 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3296 5138 882 Size: 617 1797 1842 2219 Fragments arranged by size: 2219 1842 1797 617 BsaAI yAC'GTr Cuts at: 493 1204 3772 4266 493 Size: 711 2568 494 2702 Fragments arranged by size: 2702 2568 711 494 BsaBI GATnn'nnATC Cuts at: 6469 6469 Size: 6475 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4282 4664 5299 275 Size: 53 83 186 936 2749 382 635 1451 Fragments arranged by size: 2749 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4908 5306 5580 5727 6138 4908 Size: 398 274 147 411 5245 Fragments arranged by size: 5245 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6250 Fragments arranged by size: 6250 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6475 BseYI C'CCAG_C Cuts at: 2251 2437 3707 5678 6174 2251 Size: 186 1270 1971 496 2552 Fragments arranged by size: 2552 1971 1270 496 186 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5902 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3925 4686 5714 1540 Size: 275 186 1924 761 1028 2301 Fragments arranged by size: 2301 1924 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2304 2947 4536 4621 5692 2304 Size: 643 1589 85 1071 3087 Fragments arranged by size: 3087 1589 1071 643 85 BsmI GAATG_Cn' Cuts at: 2685 3986 4079 2685 Size: 1301 93 5081 Fragments arranged by size: 5081 1301 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2304 2947 3794 4536 4621 5692 1391 Size: 399 514 643 847 742 85 1071 2174 Fragments arranged by size: 2174 1071 847 742 643 514 399 85 BspEI T'CCGG_A Cuts at: 6138 6138 Size: 6475 BspHI T'CATG_A Cuts at: 2466 3657 4364 6094 2466 Size: 1191 707 1730 2847 Fragments arranged by size: 2847 1730 1191 707 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2722 3618 3830 844 Size: 1878 896 212 3489 Fragments arranged by size: 3489 1878 896 212 BsrBI CCG'CTC Cuts at: 1394 1823 4362 1394 Size: 429 2539 3507 Fragments arranged by size: 3507 2539 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2287 4970 5144 65 Size: 1183 1039 2683 174 1396 Fragments arranged by size: 2683 1396 1183 1039 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5119 5306 1700 Size: 110 3309 187 2869 Fragments arranged by size: 3309 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2801 96 Size: 2705 3770 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6475 BssSI C'ACGA_G Cuts at: 4535 5547 4535 Size: 1012 5463 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6475 BstBI TT'CG_AA Cuts at: 2007 5279 2007 Size: 3272 3203 BstEII G'GTnAC_C Cuts at: 1184 3494 1184 Size: 2310 4165 BstXI CCAn_nnnn'nTGG Cuts at: 3353 3353 Size: 6475 BstYI r'GATC_y Cuts at: 1972 2065 2351 3270 3382 3805 4172 4557 4574 Size: 93 286 919 112 423 367 385 17 Cuts at: 4574 6026 6112 6124 6470 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 919 423 385 367 346 286 112 93 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3276 3362 3276 Size: 86 6389 BtgI C'CryG_G Cuts at: 513 1065 3346 3762 513 Size: 552 2281 416 3226 Fragments arranged by size: 3226 2281 552 416 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5736 Fragments arranged by size: 5736 683 56 BtsI GCAGTG_nn' Cuts at: 2947 3991 4785 4805 2947 Size: 1044 794 20 4617 Fragments arranged by size: 4617 1044 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6475 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6440 DraI TTT'AAA Cuts at: 3779 3779 Size: 6475 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5482 809 Size: 507 4166 1802 Fragments arranged by size: 4166 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2243 3922 4811 Size: 54 1425 50 275 18 413 1679 889 Cuts at: 4811 6440 8 Size: 1629 43 Fragments arranged by size: 1679 1629 1425 889 413 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3922 1537 Size: 275 2110 4090 Fragments arranged by size: 4090 2110 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2313 3384 3483 4405 1353 Size: 960 1071 99 922 3423 Fragments arranged by size: 3423 1071 960 922 99 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5062 5436 5582 387 Size: 1064 138 3473 374 146 1280 Fragments arranged by size: 3473 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2302 2302 Size: 6475 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2136 2176 2562 3133 3573 3590 4538 Size: 393 504 40 386 571 440 17 948 Cuts at: 4538 5135 5922 6190 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 948 787 597 571 504 440 393 386 268 40 17 EcoNI CCTnn'n_nnAGG Cuts at: 2783 2783 Size: 6475 EcoO109I rG'GnC_Cy Cuts at: 3713 3713 Size: 6475 EcoRI G'AATT_C Cuts at: 2323 3784 4162 2323 Size: 1461 378 4636 Fragments arranged by size: 4636 1461 378 FspI TGC'GCA Cuts at: 4981 4981 Size: 6475 HaeII r_GCGC'y Cuts at: 1678 5622 1678 Size: 3944 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2461 2493 3269 3301 3733 Size: 32 135 32 889 32 776 32 432 Cuts at: 3733 3765 5116 5148 5190 5222 1373 Size: 32 1351 32 42 32 2626 Fragments arranged by size: 2626 1351 889 776 432 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2227 2388 3195 3471 3819 4065 Size: 988 281 289 161 807 276 348 246 Cuts at: 4065 669 Size: 3079 Fragments arranged by size: 3079 988 807 348 289 281 276 246 161 HindIII A'AGCT_T Cuts at: 748 3338 3647 748 Size: 2590 309 3576 Fragments arranged by size: 3576 2590 309 HpaI GTT'AAC Cuts at: 3195 4065 3195 Size: 870 5605 KpnI G_GTAC'C Cuts at: 3800 3800 Size: 6475 MfeI C'AATT_G Cuts at: 4074 4074 Size: 6475 MluI A'CGCG_T Cuts at: 6351 6351 Size: 6475 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3360 5589 5773 1305 Size: 2055 2229 184 2007 Fragments arranged by size: 2229 2055 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4653 Fragments arranged by size: 4653 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4433 4792 4951 518 Size: 648 3267 359 159 2042 Fragments arranged by size: 3267 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3891 4568 5716 5961 1606 Size: 2285 677 1148 245 2120 Fragments arranged by size: 2285 2120 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 3346 3762 513 Size: 552 2281 416 3226 Fragments arranged by size: 3226 2281 552 416 NdeI CA'TA_TG Cuts at: 387 387 Size: 6475 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6475 NotI GC'GGCC_GC Cuts at: 3922 3922 Size: 6475 NspI r_CATG'y Cuts at: 1132 1229 2680 3833 5378 1132 Size: 97 1451 1153 1545 2229 Fragments arranged by size: 2229 1545 1451 1153 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6475 PciI A'CATG_T Cuts at: 5374 5374 Size: 6475 PflMI CCAn_nnn'nTGG Cuts at: 1108 2258 3068 1108 Size: 1150 810 4515 Fragments arranged by size: 4515 1150 810 PmeI GTTT'AAAC Cuts at: 3779 3779 Size: 6475 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4544 4576 6057 6089 4544 Size: 32 1481 32 4930 Fragments arranged by size: 4930 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6475 PsiI TTA'TAA Cuts at: 4045 4045 Size: 6475 PstI C_TGCA'G Cuts at: 830 3644 3827 830 Size: 2814 183 3478 Fragments arranged by size: 3478 2814 183 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6475 SacI G_AGCT'C Cuts at: 2304 2304 Size: 6475 SalI G'TCGA_C Cuts at: 1936 3817 1936 Size: 1881 4594 SbfI CC_TGCA'GG Cuts at: 3827 3827 Size: 6475 ScaI AGT'ACT Cuts at: 4723 6435 4723 Size: 1712 4763 SfcI C'TryA_G Cuts at: 826 1045 2859 3640 3823 4958 5639 5830 826 Size: 219 1814 781 183 1135 681 191 1471 Fragments arranged by size: 1814 1471 1135 781 681 219 191 183 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6475 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6475 SmaI CCC'GGG Cuts at: 1872 3802 1872 Size: 1930 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3302 3431 3626 4579 5480 5742 Size: 197 926 1359 129 195 953 901 262 Cuts at: 5742 820 Size: 1553 Fragments arranged by size: 1553 1359 953 926 901 262 197 195 129 SnaBI TAC'GTA Cuts at: 493 3772 4266 493 Size: 3279 494 2702 Fragments arranged by size: 3279 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 6475 SphI G_CATG'C Cuts at: 1132 2680 3833 1132 Size: 1548 1153 3774 Fragments arranged by size: 3774 1548 1153 SspI AAT'ATT Cuts at: 5 52 4399 5 Size: 47 4347 2081 Fragments arranged by size: 4347 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6475 StyI C'CwwG_G Cuts at: 513 1065 2085 3207 3346 3523 3762 3914 513 Size: 552 1020 1122 139 177 239 152 3074 Fragments arranged by size: 3074 1122 1020 552 239 177 152 139 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3056 4502 4519 4672 4857 5302 325 Size: 1255 1476 1446 17 153 185 445 1498 Fragments arranged by size: 1498 1476 1446 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2801 4721 6433 Size: 274 80 33 51 1443 824 1920 1712 Cuts at: 6433 96 Size: 138 Fragments arranged by size: 1920 1712 1443 824 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6435 6467 6435 Size: 32 6443 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6475 XbaI T'CTAG_A Cuts at: 3385 3811 3385 Size: 426 6049 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6475 XmaI C'CCGG_G Cuts at: 1870 3800 1870 Size: 1930 4545 XmnI GAAnn'nnTTC Cuts at: 2778 4604 2778 Size: 1826 4649 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4283 276 Size: 53 83 186 936 2749 2468 Fragments arranged by size: 2749 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BsmBI BstZ17I DraIII EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI