(Circular) (Six-base) MAPSORT of: pFN21AE2503.seq Check: 3702 from: 1 to: 6598 pFN21AE2503 6598 bp With 182 enzymes: SgfI * February 16, 2012 14:31 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4408 278 Size: 53 83 186 936 2872 2468 Fragments arranged by size: 2872 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2347 3941 1937 Size: 410 1594 4594 Fragments arranged by size: 4594 1594 410 Acc65I G'GTAC_C Cuts at: 3919 3919 Size: 6598 AclI AA'CG_TT Cuts at: 1563 2578 4725 5098 1563 Size: 1015 2147 373 3063 Fragments arranged by size: 3063 2147 1015 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2117 3098 3140 3215 4661 6045 6313 1632 Size: 485 981 42 75 1446 1384 268 1917 Fragments arranged by size: 1917 1446 1384 981 485 268 75 42 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6401 AflIII A'CryG_T Cuts at: 1203 2731 5497 6474 1203 Size: 1528 2766 977 1327 Fragments arranged by size: 2766 1528 1327 977 AgeI A'CCGG_T Cuts at: 5429 5429 Size: 6598 AhdI GACnn_n'nnGTC Cuts at: 5327 5327 Size: 6598 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6598 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6598 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2315 2347 2315 Size: 32 6566 AlwNI CAG_nnn'CTG Cuts at: 2051 2719 3163 3326 3849 4341 5913 2051 Size: 668 444 163 523 492 1572 2736 Fragments arranged by size: 2736 1572 668 523 492 444 163 ApaI G_GGCC'C Cuts at: 2815 2815 Size: 6598 ApaLI G'TGCA_C Cuts at: 4655 5811 4655 Size: 1156 5442 ApoI r'AATT_y Cuts at: 1493 3608 3907 4133 4285 6394 1493 Size: 2115 299 226 152 2109 1697 Fragments arranged by size: 2115 2109 1697 299 226 152 AseI AT'TA_AT Cuts at: 160 1032 5152 160 Size: 872 4120 1606 Fragments arranged by size: 4120 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6598 AvaI C'yCGr_G Cuts at: 1870 1943 2326 2659 3674 3923 1870 Size: 73 383 333 1015 249 4545 Fragments arranged by size: 4545 1015 383 333 249 73 BamHI G'GATC_C Cuts at: 3928 4295 3928 Size: 367 6231 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2183 3919 5374 618 Size: 325 217 627 396 1736 1455 1842 Fragments arranged by size: 1842 1736 1455 627 396 325 217 BanII G_rGCy'C Cuts at: 1391 2045 2297 2815 3030 3658 3675 3917 1391 Size: 654 252 518 215 628 17 242 4072 Fragments arranged by size: 4072 654 628 518 252 242 215 17 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5627 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4789 4823 1903 Size: 34 2852 34 3678 Fragments arranged by size: 3678 2852 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4490 5700 4490 Size: 1210 5388 BclI T'GATC_A Cuts at: 1548 1548 Size: 6598 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5209 136 Size: 107 122 71 631 605 3537 1525 Fragments arranged by size: 3537 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6593 6593 Size: 6598 BlpI GC'TnA_GC Cuts at: 2940 4015 2940 Size: 1075 5523 Bme1580I G_kGCm'C Cuts at: 1790 2188 2815 3603 4659 5815 1790 Size: 398 627 788 1056 1156 2573 Fragments arranged by size: 2573 1156 1056 788 627 398 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6598 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2189 2809 3336 Size: 416 53 83 399 24 772 620 527 Cuts at: 3336 3639 4329 5287 442 Size: 303 690 958 1753 Fragments arranged by size: 1753 958 772 690 620 527 416 399 303 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6598 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2126 2792 2924 3014 3029 3380 3573 5258 Size: 887 666 132 90 15 351 193 1685 Cuts at: 5258 1239 Size: 2579 Fragments arranged by size: 2579 1685 887 666 351 193 132 90 15 Bpu10I CC'TnA_GC Cuts at: 3036 3036 Size: 6598 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2666 4723 5588 5886 2666 Size: 2057 865 298 3378 Fragments arranged by size: 3378 2057 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2616 5261 882 Size: 617 1117 2645 2219 Fragments arranged by size: 2645 2219 1117 617 BsaAI yAC'GTr Cuts at: 493 1204 2734 4389 493 Size: 711 1530 1655 2702 Fragments arranged by size: 2702 1655 1530 711 BsaBI GATnn'nnATC Cuts at: 2886 6592 2886 Size: 3706 2892 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4405 4787 5422 275 Size: 53 83 186 936 2872 382 635 1451 Fragments arranged by size: 2872 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3420 5031 5429 5703 5850 6261 3420 Size: 1611 398 274 147 411 3757 Fragments arranged by size: 3757 1611 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2119 2149 3555 3585 1178 Size: 30 165 30 716 30 1406 30 4191 Fragments arranged by size: 4191 1406 716 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2153 2251 2260 2263 2882 2912 2923 3119 Size: 967 98 9 3 619 30 11 196 Cuts at: 3119 3203 3377 3513 3546 1186 Size: 84 174 136 33 4238 Fragments arranged by size: 4238 967 619 196 174 136 98 84 33 30 11 9 3 BseYI C'CCAG_C Cuts at: 2219 2962 3256 3313 3658 3870 5801 6297 2219 Size: 743 294 57 345 212 1931 496 2520 Fragments arranged by size: 2520 1931 743 496 345 294 212 57 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2097 2152 3720 3765 3815 1163 Size: 573 361 55 1568 45 50 3946 Fragments arranged by size: 3946 1568 573 361 55 50 45 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2569 3288 4048 4809 5837 1540 Size: 275 186 568 719 760 761 1028 2301 Fragments arranged by size: 2301 1028 761 760 719 568 275 186 BsiHKAI G_wGCw'C Cuts at: 3030 3675 3831 4659 4744 5815 3030 Size: 645 156 828 85 1071 3813 Fragments arranged by size: 3813 1071 828 645 156 85 BsmI GAATG_Cn' Cuts at: 4109 4202 4109 Size: 93 6505 BsmBI CGTCTCn'nnnn_ Cuts at: 3266 3266 Size: 6598 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2045 2188 2297 2815 3030 3603 3658 Size: 399 255 143 109 518 215 573 55 Cuts at: 3658 3675 3831 3917 4659 4744 5815 1391 Size: 17 156 86 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 573 518 399 255 215 156 143 109 86 85 55 17 BspEI T'CCGG_A Cuts at: 6261 6261 Size: 6598 BspHI T'CATG_A Cuts at: 4487 6217 4487 Size: 1730 4868 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3953 844 Size: 3109 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4485 1394 Size: 429 2662 3507 Fragments arranged by size: 3507 2662 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5093 5267 65 Size: 1183 3845 174 1396 Fragments arranged by size: 3845 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2564 5242 5429 1700 Size: 110 754 2678 187 2869 Fragments arranged by size: 2869 2678 754 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6598 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6598 BssSI C'ACGA_G Cuts at: 3200 4658 5670 3200 Size: 1458 1012 4128 Fragments arranged by size: 4128 1458 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2719 3849 794 Size: 1925 1130 3543 Fragments arranged by size: 3543 1925 1130 BstBI TT'CG_AA Cuts at: 5402 5402 Size: 6598 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6598 BstXI CCAn_nnnn'nTGG Cuts at: 2560 3727 2560 Size: 1167 5431 BstYI r'GATC_y Cuts at: 1972 2101 2557 3928 4295 4680 4697 6149 6235 Size: 129 456 1371 367 385 17 1452 86 Cuts at: 6235 6247 6593 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1371 456 385 367 346 129 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2996 3319 3377 2996 Size: 323 58 6217 Fragments arranged by size: 6217 323 58 BtgI C'CryG_G Cuts at: 513 1065 3073 513 Size: 552 2008 4038 Fragments arranged by size: 4038 2008 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2568 3434 487 Size: 683 56 1342 866 3651 Fragments arranged by size: 3651 1342 866 683 56 BtsI GCAGTG_nn' Cuts at: 2140 2945 2973 3215 4114 4908 4928 2140 Size: 805 28 242 899 794 20 3810 Fragments arranged by size: 3810 899 805 794 242 28 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6598 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6563 DraI TTT'AAA Cuts at: 3902 3902 Size: 6598 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5605 809 Size: 507 4289 1802 Fragments arranged by size: 4289 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2562 2566 3165 Size: 54 1425 50 275 18 732 4 599 Cuts at: 3165 3285 3615 4045 4934 6563 8 Size: 120 330 430 889 1629 43 Fragments arranged by size: 1629 1425 889 732 599 430 330 275 120 54 50 43 18 4 EagI C'GGCC_G Cuts at: 1537 1812 2566 3285 4045 1537 Size: 275 754 719 760 4090 Fragments arranged by size: 4090 760 754 719 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2541 3143 4528 1353 Size: 1188 602 1385 3423 Fragments arranged by size: 3423 1385 1188 602 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2140 2254 3386 5185 5559 5705 Size: 1064 138 551 114 1132 1799 374 146 Cuts at: 5705 387 Size: 1280 Fragments arranged by size: 1799 1280 1132 1064 551 374 146 138 114 EcoICRI GAG'CTC Cuts at: 3028 3673 3028 Size: 645 5953 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2117 2126 2792 2924 3014 3029 3098 Size: 393 485 9 666 132 90 15 69 Cuts at: 3098 3140 3215 3380 3573 4661 5258 6045 6313 Size: 42 75 165 193 1088 597 787 268 Cuts at: 6313 1239 Size: 1524 Fragments arranged by size: 1524 1088 787 666 597 485 393 268 193 165 132 90 75 69 42 15 9 EcoNI CCTnn'n_nnAGG Cuts at: 2999 3359 3738 2999 Size: 360 379 5859 Fragments arranged by size: 5859 379 360 EcoO109I rG'GnC_Cy Cuts at: 2811 2811 Size: 6598 EcoRI G'AATT_C Cuts at: 3907 4285 3907 Size: 378 6220 FseI GG_CCGG'CC Cuts at: 2568 2568 Size: 6598 FspI TGC'GCA Cuts at: 5104 5104 Size: 6598 HaeII r_GCGC'y Cuts at: 1678 5745 1678 Size: 4067 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2051 2083 2642 2674 3421 Size: 32 135 32 479 32 559 32 747 Cuts at: 3421 3453 5239 5271 5313 5345 1373 Size: 32 1786 32 42 32 2626 Fragments arranged by size: 2626 1786 747 559 479 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2348 3942 4188 669 Size: 988 281 410 1594 246 3079 Fragments arranged by size: 3079 1594 988 410 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6598 HpaI GTT'AAC Cuts at: 4188 4188 Size: 6598 KpnI G_GTAC'C Cuts at: 3923 3923 Size: 6598 MfeI C'AATT_G Cuts at: 4197 4197 Size: 6598 MluI A'CGCG_T Cuts at: 6474 6474 Size: 6598 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2016 2181 3204 5712 5896 1305 Size: 711 165 1023 2508 184 2007 Fragments arranged by size: 2508 2007 1023 711 184 165 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4776 Fragments arranged by size: 4776 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2071 4556 4915 5074 518 Size: 648 905 2485 359 159 2042 Fragments arranged by size: 2485 2042 905 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2173 2198 2404 2770 3163 3620 4014 4691 Size: 567 25 206 366 393 457 394 677 Cuts at: 4691 5839 6084 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1148 677 567 457 394 393 366 245 206 25 NaeI GCC'GGC Cuts at: 2566 2566 Size: 6598 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 6046 NdeI CA'TA_TG Cuts at: 387 387 Size: 6598 NgoMIV G'CCGG_C Cuts at: 2564 2564 Size: 6598 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6598 NotI GC'GGCC_GC Cuts at: 4045 4045 Size: 6598 NruI TCG'CGA Cuts at: 3096 3096 Size: 6598 NspI r_CATG'y Cuts at: 1132 1229 2285 3389 3956 5501 1132 Size: 97 1056 1104 567 1545 2229 Fragments arranged by size: 2229 1545 1104 1056 567 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6598 PciI A'CATG_T Cuts at: 5497 5497 Size: 6598 PflMI CCAn_nnn'nTGG Cuts at: 1108 2018 3268 1108 Size: 910 1250 4438 Fragments arranged by size: 4438 1250 910 PfoI T'CCnGG_A Cuts at: 3357 3649 3357 Size: 292 6306 PmeI GTTT'AAAC Cuts at: 3902 3902 Size: 6598 PmlI CAC'GTG Cuts at: 2734 2734 Size: 6598 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4667 4699 6180 6212 4667 Size: 32 1481 32 5053 Fragments arranged by size: 5053 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6598 PsiI TTA'TAA Cuts at: 4168 4168 Size: 6598 PspOMI G'GGCC_C Cuts at: 2811 2811 Size: 6598 PspXI vC'TCGA_Gb Cuts at: 2326 2326 Size: 6598 PstI C_TGCA'G Cuts at: 830 3222 3249 3562 3950 830 Size: 2392 27 313 388 3478 Fragments arranged by size: 3478 2392 388 313 27 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6598 PvuII CAG'CTG Cuts at: 2198 2404 2770 3163 2198 Size: 206 366 393 5633 Fragments arranged by size: 5633 393 366 206 SacI G_AGCT'C Cuts at: 3030 3675 3030 Size: 645 5953 SalI G'TCGA_C Cuts at: 1936 2346 3940 1936 Size: 410 1594 4594 Fragments arranged by size: 4594 1594 410 SbfI CC_TGCA'GG Cuts at: 3950 3950 Size: 6598 ScaI AGT'ACT Cuts at: 4846 6558 4846 Size: 1712 4886 SfcI C'TryA_G Cuts at: 826 1045 3218 3245 3558 3946 5081 5762 5953 Size: 219 2173 27 313 388 1135 681 191 Cuts at: 5953 826 Size: 1471 Fragments arranged by size: 2173 1471 1135 681 388 313 219 191 27 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6598 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6598 SmaI CCC'GGG Cuts at: 1872 3925 1872 Size: 2053 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2326 2659 2681 4702 5603 5865 Size: 197 926 383 333 22 2021 901 262 Cuts at: 5865 820 Size: 1553 Fragments arranged by size: 2021 1553 926 901 383 333 262 197 22 SnaBI TAC'GTA Cuts at: 493 4389 493 Size: 3896 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6598 SphI G_CATG'C Cuts at: 1132 2285 3956 1132 Size: 1153 1671 3774 Fragments arranged by size: 3774 1671 1153 SspI AAT'ATT Cuts at: 5 52 4522 5 Size: 47 4470 2081 Fragments arranged by size: 4470 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6598 StyI C'CwwG_G Cuts at: 513 1065 2553 3802 4037 513 Size: 552 1488 1249 235 3074 Fragments arranged by size: 3074 1488 1249 552 235 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2171 3846 4625 4642 4795 4980 5425 Size: 1255 591 1675 779 17 153 185 445 Cuts at: 5425 325 Size: 1498 Fragments arranged by size: 1675 1498 1255 779 591 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4844 6556 96 Size: 274 80 33 51 1443 2867 1712 138 Fragments arranged by size: 2867 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2119 2151 6558 6590 2119 Size: 32 4407 32 2127 Fragments arranged by size: 4407 2127 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6598 XbaI T'CTAG_A Cuts at: 3934 3934 Size: 6598 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3512 3867 3512 Size: 355 6243 XhoI C'TCGA_G Cuts at: 1943 2326 2659 1943 Size: 383 333 5882 Fragments arranged by size: 5882 383 333 XmaI C'CCGG_G Cuts at: 1870 3923 1870 Size: 2053 4545 XmnI GAAnn'nnTTC Cuts at: 4727 4727 Size: 6598 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4406 276 Size: 53 83 186 936 2872 2468 Fragments arranged by size: 2872 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FseI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NruI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BstZ17I DraIII EcoRV FalI FspAI KasI NarI NsiI PacI PpuMI PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI