(Circular) (Six-base) MAPSORT of: pFN21ASDA0854.seq Check: 2483 from: 1 to: 7240 pFN21ASDA0854 7240 bp With 182 enzymes: SgfI * January 16, 2009 15:30 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5050 278 Size: 53 83 186 936 3514 2468 Fragments arranged by size: 3514 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2103 2313 4583 1937 Size: 166 210 2270 4594 Fragments arranged by size: 4594 2270 210 166 Acc65I G'GTAC_C Cuts at: 4561 4561 Size: 7240 AclI AA'CG_TT Cuts at: 1563 5367 5740 1563 Size: 3804 373 3063 Fragments arranged by size: 3804 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2465 5303 6687 6955 1632 Size: 833 2838 1384 268 1917 Fragments arranged by size: 2838 1917 1384 833 268 AfeI AGC'GCT Cuts at: 2963 2963 Size: 7240 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7043 AflIII A'CryG_T Cuts at: 1203 2352 2743 6139 7116 1203 Size: 1149 391 3396 977 1327 Fragments arranged by size: 3396 1327 1149 977 391 AgeI A'CCGG_T Cuts at: 4332 6071 4332 Size: 1739 5501 AhdI GACnn_n'nnGTC Cuts at: 5969 5969 Size: 7240 AleI CACnn'nnGTG Cuts at: 1166 3089 1166 Size: 1923 5317 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7240 AlwNI CAG_nnn'CTG Cuts at: 2946 4523 4983 6555 2946 Size: 1577 460 1572 3631 Fragments arranged by size: 3631 1577 1572 460 ApaLI G'TGCA_C Cuts at: 5297 6453 5297 Size: 1156 6084 ApoI r'AATT_y Cuts at: 1493 4549 4775 4927 7036 1493 Size: 3056 226 152 2109 1697 Fragments arranged by size: 3056 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 5794 160 Size: 872 4762 1606 Fragments arranged by size: 4762 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7240 AvaI C'yCGr_G Cuts at: 1870 1943 2093 2423 2630 2645 4565 1870 Size: 73 150 330 207 15 1920 4545 Fragments arranged by size: 4545 1920 330 207 150 73 15 BamHI G'GATC_C Cuts at: 4570 4937 4570 Size: 367 6873 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2617 3029 3298 3878 4561 Size: 325 217 627 830 412 269 580 683 Cuts at: 4561 6016 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 830 683 627 580 412 325 269 217 BanII G_rGCy'C Cuts at: 1391 2724 3309 3841 4181 4559 1391 Size: 1333 585 532 340 378 4072 Fragments arranged by size: 4072 1333 585 532 378 340 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3365 3663 928 Size: 971 1466 298 4505 Fragments arranged by size: 4505 1466 971 298 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3521 3555 5431 5465 1903 Size: 34 1584 34 1876 34 3678 Fragments arranged by size: 3678 1876 1584 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2830 5132 6342 2830 Size: 2302 1210 3728 Fragments arranged by size: 3728 2302 1210 BclI T'GATC_A Cuts at: 1548 2853 4126 1548 Size: 1305 1273 4662 Fragments arranged by size: 4662 1305 1273 BfrBI ATG'CAT Cuts at: 2055 2055 Size: 7240 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3077 3563 4356 Size: 107 122 71 631 605 1405 486 793 Cuts at: 4356 5851 136 Size: 1495 1525 Fragments arranged by size: 1525 1495 1405 793 631 605 486 122 107 71 BglII A'GATC_T Cuts at: 7235 7235 Size: 7240 BlpI GC'TnA_GC Cuts at: 3725 4657 3725 Size: 932 6308 Bme1580I G_kGCm'C Cuts at: 1790 2094 2622 2790 3883 5301 6457 1790 Size: 304 528 168 1093 1418 1156 2573 Fragments arranged by size: 2573 1418 1156 1093 528 304 168 BmgBI CAC'GTC Cuts at: 1318 2146 2746 1318 Size: 828 600 5812 Fragments arranged by size: 5812 828 600 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3067 3877 3967 Size: 416 53 83 399 24 1650 810 90 Cuts at: 3967 4971 5929 442 Size: 1004 958 1753 Fragments arranged by size: 1753 1650 1004 958 810 416 399 90 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2031 1056 Size: 975 6265 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2716 3760 4342 2716 Size: 1044 582 5614 Fragments arranged by size: 5614 1044 582 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3092 3383 4474 5900 1239 Size: 1853 291 1091 1426 2579 Fragments arranged by size: 2579 1853 1426 1091 291 Bpu10I CC'TnA_GC Cuts at: 3294 3707 3294 Size: 413 6827 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2686 3368 5365 6230 6528 2686 Size: 682 1997 865 298 3398 Fragments arranged by size: 3398 1997 865 682 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3235 3710 5903 882 Size: 617 1736 475 2193 2219 Fragments arranged by size: 2219 2193 1736 617 475 BsaAI yAC'GTr Cuts at: 493 1204 2353 2656 3585 4402 4537 5031 493 Size: 711 1149 303 929 817 135 494 2702 Fragments arranged by size: 2702 1149 929 817 711 494 303 135 BsaBI GATnn'nnATC Cuts at: 7234 7234 Size: 7240 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 5047 5429 6064 275 Size: 53 83 186 936 3514 382 635 1451 Fragments arranged by size: 3514 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3875 4332 5673 6071 6345 6492 6903 3875 Size: 457 1341 398 274 147 411 4212 Fragments arranged by size: 4212 1341 457 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2393 2423 3857 3887 1178 Size: 30 165 30 990 30 1434 30 4531 Fragments arranged by size: 4531 1434 990 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2648 2714 2945 3017 3967 4394 4436 1186 Size: 1462 66 231 72 950 427 42 3990 Fragments arranged by size: 3990 1462 950 427 231 72 66 42 BseYI C'CCAG_C Cuts at: 2958 3902 6443 6939 2958 Size: 944 2541 496 3259 Fragments arranged by size: 3259 2541 944 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2379 1163 Size: 573 643 6024 Fragments arranged by size: 6024 643 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3580 4416 4690 5451 6479 1540 Size: 275 186 1579 836 274 761 1028 2301 Fragments arranged by size: 2301 1579 1028 836 761 275 274 186 BsiHKAI G_wGCw'C Cuts at: 2724 2937 3853 3959 5301 5386 6457 2724 Size: 213 916 106 1342 85 1071 3507 Fragments arranged by size: 3507 1342 1071 916 213 106 85 BsmI GAATG_Cn' Cuts at: 4751 4844 4751 Size: 93 7147 BsmBI CGTCTCn'nnnn_ Cuts at: 3190 3190 Size: 7240 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2094 2622 2724 2790 2937 3309 3841 Size: 399 304 528 102 66 147 372 532 Cuts at: 3841 3853 3883 3959 4181 4559 5301 5386 6457 Size: 12 30 76 222 378 742 85 1071 Cuts at: 6457 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 532 528 399 378 372 304 222 147 102 85 76 66 30 12 BspEI T'CCGG_A Cuts at: 6903 6903 Size: 7240 BspHI T'CATG_A Cuts at: 2580 5129 6859 2580 Size: 2549 1730 2961 Fragments arranged by size: 2961 2549 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4595 844 Size: 3751 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3334 5127 1394 Size: 429 1511 1793 3507 Fragments arranged by size: 3507 1793 1511 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5735 5909 65 Size: 1183 4487 174 1396 Fragments arranged by size: 4487 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3432 4332 5884 6071 1700 Size: 110 1622 900 1552 187 2869 Fragments arranged by size: 2869 1622 1552 900 187 110 BsrGI T'GTAC_A Cuts at: 96 2758 96 Size: 2662 4578 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7240 BssSI C'ACGA_G Cuts at: 5300 6312 5300 Size: 1012 6228 BstAPI GCAn_nnn'nTGC Cuts at: 794 2268 794 Size: 1474 5766 BstBI TT'CG_AA Cuts at: 2007 6044 2007 Size: 4037 3203 BstEII G'GTnAC_C Cuts at: 1184 3163 4258 1184 Size: 1979 1095 4166 Fragments arranged by size: 4166 1979 1095 BstYI r'GATC_y Cuts at: 1972 2556 2694 3129 4570 4937 5322 5339 6791 Size: 584 138 435 1441 367 385 17 1452 Cuts at: 6791 6877 6889 7235 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 1441 584 435 385 367 346 138 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3950 3950 Size: 7240 BtgI C'CryG_G Cuts at: 513 1065 2022 3210 3229 3804 4116 4167 513 Size: 552 957 1188 19 575 312 51 3586 Fragments arranged by size: 3586 1188 957 575 552 312 51 19 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3274 3544 487 Size: 683 56 2048 270 4183 Fragments arranged by size: 4183 2048 683 270 56 BtsI GCAGTG_nn' Cuts at: 4756 5550 5570 4756 Size: 794 20 6426 Fragments arranged by size: 6426 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7240 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7205 DraI TTT'AAA Cuts at: 4544 4544 Size: 7240 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3172 6247 809 Size: 507 1856 3075 1802 Fragments arranged by size: 3075 1856 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2148 3136 3232 Size: 54 1425 50 275 18 318 988 96 Cuts at: 3232 3321 3555 4008 4170 4357 4525 4687 5576 Size: 89 234 453 162 187 168 162 889 Cuts at: 5576 7205 8 Size: 1629 43 Fragments arranged by size: 1629 1425 988 889 453 318 275 234 187 168 162 162 96 89 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4687 1537 Size: 275 2875 4090 Fragments arranged by size: 4090 2875 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2188 2988 3233 4233 5170 1353 Size: 835 800 245 1000 937 3423 Fragments arranged by size: 3423 1000 937 835 800 245 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3778 5827 6201 6347 387 Size: 1064 138 2189 2049 374 146 1280 Fragments arranged by size: 2189 2049 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2722 2722 Size: 7240 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2465 3092 3383 4474 5303 5900 6687 Size: 393 833 627 291 1091 829 597 787 Cuts at: 6687 6955 1239 Size: 268 1524 Fragments arranged by size: 1524 1091 833 829 787 627 597 393 291 268 EcoO109I rG'GnC_Cy Cuts at: 2794 4219 2794 Size: 1425 5815 EcoRI G'AATT_C Cuts at: 4549 4927 4549 Size: 378 6862 EcoRV GAT'ATC Cuts at: 3497 3497 Size: 7240 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3262 3409 3262 Size: 147 7093 FspI TGC'GCA Cuts at: 5746 5746 Size: 7240 HaeII r_GCGC'y Cuts at: 1678 2965 6387 1678 Size: 1287 3422 2531 Fragments arranged by size: 3422 2531 1287 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2198 2230 2381 2393 2413 Size: 32 135 32 626 32 151 12 20 Cuts at: 2413 2425 3364 3396 3857 3889 4418 4450 5881 Size: 12 939 32 461 32 529 32 1431 Cuts at: 5881 5913 5955 5987 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1431 939 626 529 461 151 135 42 32 32 32 32 32 32 32 32 20 12 12 HincII GTy'rAC Cuts at: 669 1657 1938 2314 3177 3888 4584 4830 669 Size: 988 281 376 863 711 696 246 3079 Fragments arranged by size: 3079 988 863 711 696 376 281 246 HindIII A'AGCT_T Cuts at: 748 3767 748 Size: 3019 4221 HpaI GTT'AAC Cuts at: 3888 4830 3888 Size: 942 6298 KpnI G_GTAC'C Cuts at: 4565 4565 Size: 7240 MfeI C'AATT_G Cuts at: 3918 4839 3918 Size: 921 6319 MluI A'CGCG_T Cuts at: 7116 7116 Size: 7240 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2424 4461 4527 6354 6538 1305 Size: 1119 2037 66 1827 184 2007 Fragments arranged by size: 2037 2007 1827 1119 184 66 MscI TGG'CCA Cuts at: 10 64 1489 1832 2150 3234 3323 3557 4527 Size: 54 1425 343 318 1084 89 234 970 Cuts at: 4527 10 Size: 2723 Fragments arranged by size: 2723 1425 1084 970 343 318 234 89 54 MslI CAynn'nnrTG Cuts at: 518 1166 3059 3089 3443 5198 5557 5716 518 Size: 648 1893 30 354 1755 359 159 2042 Fragments arranged by size: 2042 1893 1755 648 359 354 159 30 MspA1I CmG'CkG Cuts at: 1606 2690 2764 2912 2982 3171 4656 5333 6481 Size: 1084 74 148 70 189 1485 677 1148 Cuts at: 6481 6726 1606 Size: 245 2120 Fragments arranged by size: 2120 1485 1148 1084 677 245 189 148 74 70 NcoI C'CATG_G Cuts at: 513 1065 2022 3229 3804 4116 4167 513 Size: 552 957 1207 575 312 51 3586 Fragments arranged by size: 3586 1207 957 575 552 312 51 NdeI CA'TA_TG Cuts at: 387 387 Size: 7240 NheI G'CTAG_C Cuts at: 1052 2027 1052 Size: 975 6265 NotI GC'GGCC_GC Cuts at: 4687 4687 Size: 7240 NsiI A_TGCA'T Cuts at: 2057 2057 Size: 7240 NspI r_CATG'y Cuts at: 1132 1229 2312 2320 4598 6143 1132 Size: 97 1083 8 2278 1545 2229 Fragments arranged by size: 2278 2229 1545 1083 97 8 PacI TTA_AT'TAA Cuts at: 2454 2454 Size: 7240 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7240 PciI A'CATG_T Cuts at: 6139 6139 Size: 7240 PflMI CCAn_nnn'nTGG Cuts at: 1108 3832 4117 1108 Size: 2724 285 4231 Fragments arranged by size: 4231 2724 285 PmeI GTTT'AAAC Cuts at: 4544 4544 Size: 7240 PmlI CAC'GTG Cuts at: 2656 2656 Size: 7240 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5309 5341 6822 6854 5309 Size: 32 1481 32 5695 Fragments arranged by size: 5695 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2794 4219 2794 Size: 1425 5815 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7240 PsiI TTA'TAA Cuts at: 4810 4810 Size: 7240 PstI C_TGCA'G Cuts at: 830 4519 4592 830 Size: 3689 73 3478 Fragments arranged by size: 3689 3478 73 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7240 PvuII CAG'CTG Cuts at: 2690 2764 2912 2982 2690 Size: 74 148 70 6948 Fragments arranged by size: 6948 148 74 70 SacI G_AGCT'C Cuts at: 2724 2724 Size: 7240 SalI G'TCGA_C Cuts at: 1936 2312 4582 1936 Size: 376 2270 4594 Fragments arranged by size: 4594 2270 376 SbfI CC_TGCA'GG Cuts at: 4592 4592 Size: 7240 ScaI AGT'ACT Cuts at: 5488 7200 5488 Size: 1712 5528 SfcI C'TryA_G Cuts at: 826 1045 4515 4588 5723 6404 6595 826 Size: 219 3470 73 1135 681 191 1471 Fragments arranged by size: 3470 1471 1135 681 219 191 73 SfiI GGCCn_nnn'nGGCC Cuts at: 3563 3563 Size: 7240 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7240 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7240 SmaI CCC'GGG Cuts at: 1872 2425 4567 1872 Size: 553 2142 4545 Fragments arranged by size: 4545 2142 553 SmlI C'TyrA_G Cuts at: 820 1017 1943 2701 3383 5344 6245 6507 820 Size: 197 926 758 682 1961 901 262 1553 Fragments arranged by size: 1961 1553 926 901 758 682 262 197 SnaBI TAC'GTA Cuts at: 493 4537 5031 493 Size: 4044 494 2702 Fragments arranged by size: 4044 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 7240 SphI G_CATG'C Cuts at: 1132 4598 1132 Size: 3466 3774 SspI AAT'ATT Cuts at: 5 52 4211 5164 5 Size: 47 4159 953 2081 Fragments arranged by size: 4159 2081 953 47 StuI AGG'CCT Cuts at: 1517 4532 1517 Size: 3015 4225 StyI C'CwwG_G Cuts at: 513 1065 2022 2151 2245 2790 3229 3246 3315 Size: 552 957 129 94 545 439 17 69 Cuts at: 3315 3804 4116 4167 4679 513 Size: 489 312 51 512 3074 Fragments arranged by size: 3074 957 552 545 512 489 439 312 129 94 69 51 17 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2940 2972 3328 5267 5284 5437 5622 Size: 1255 1360 32 356 1939 17 153 185 Cuts at: 5622 6067 325 Size: 445 1498 Fragments arranged by size: 1939 1498 1360 1255 445 356 185 153 32 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2758 5486 7198 Size: 274 80 33 51 1443 781 2728 1712 Cuts at: 7198 96 Size: 138 Fragments arranged by size: 2728 1712 1443 781 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 7200 7232 7200 Size: 32 7208 Tth111I GACn'n_nGTC Cuts at: 1316 3576 4508 1316 Size: 2260 932 4048 Fragments arranged by size: 4048 2260 932 XbaI T'CTAG_A Cuts at: 4576 4576 Size: 7240 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3550 4018 3550 Size: 468 6772 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7240 XmaI C'CCGG_G Cuts at: 1870 2423 4565 1870 Size: 553 2142 4545 Fragments arranged by size: 4545 2142 553 XmnI GAAnn'nnTTC Cuts at: 2297 3020 3479 3939 5369 2297 Size: 723 459 460 1430 4168 Fragments arranged by size: 4168 1430 723 460 459 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5048 276 Size: 53 83 186 936 3514 2468 Fragments arranged by size: 3514 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PacI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI AvrII BaeI BbeI BbvCI BsiWI BstXI BstZ17I DraIII EcoNI FseI FspAI KasI NaeI NarI NgoMIV NruI PfoI PspOMI PspXI PsrI RsrII SacII SanDI SapI SexAI SfoI SrfI SwaI