Restriction map for
pFN21ASDA0854
(Linear) (Six-base) MAPSORT of: KIAA0854.seq Check: 7138 from: 1 to: 2511
KIAA0854.orf
With 182 enzymes: SgfI *
October 24, 2006 15:14 ..
AccI GT'mk_AC
Cuts at: 0 80 290 2511
Size: 80 210 2221
Fragments arranged by size:
2221 210 80
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 442 2511
Size: 442 2069
AfeI AGC'GCT
Cuts at: 0 940 2511
Size: 940 1571
AflIII A'CryG_T
Cuts at: 0 329 720 2511
Size: 329 391 1791
Fragments arranged by size:
1791 391 329
AgeI A'CCGG_T
Cuts at: 0 2309 2511
Size: 2309 202
AleI CACnn'nnGTG
Cuts at: 0 1066 2511
Size: 1066 1445
AlwNI CAG_nnn'CTG
Cuts at: 0 923 2500 2511
Size: 923 1577 11
Fragments arranged by size:
1577 923 11
AvaI C'yCGr_G
Cuts at: 0 70 400 607 622 2511
Size: 70 330 207 15 1889
Fragments arranged by size:
1889 330 207 70 15
BanI G'GyrC_C
Cuts at: 0 594 1006 1275 1855 2511
Size: 594 412 269 580 656
Fragments arranged by size:
656 594 580 412 269
BanII G_rGCy'C
Cuts at: 0 701 1286 1818 2158 2511
Size: 701 585 532 340 353
Fragments arranged by size:
701 585 532 353 340
BbsI GAAGACnn'nnnn_
Cuts at: 0 1342 1640 2511
Size: 1342 298 871
Fragments arranged by size:
1342 871 298
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1498 1532 2511
Size: 1498 34 979
Fragments arranged by size:
1498 979 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 807 2511
Size: 807 1704
BclI T'GATC_A
Cuts at: 0 830 2103 2511
Size: 830 1273 408
Fragments arranged by size:
1273 830 408
BfrBI ATG'CAT
Cuts at: 0 32 2511
Size: 32 2479
BglI GCCn_nnn'nGGC
Cuts at: 0 1054 1540 2333 2511
Size: 1054 486 793 178
Fragments arranged by size:
1054 793 486 178
BlpI GC'TnA_GC
Cuts at: 0 1702 2511
Size: 1702 809
Bme1580I G_kGCm'C
Cuts at: 0 71 599 767 1860 2511
Size: 71 528 168 1093 651
Fragments arranged by size:
1093 651 528 168 71
BmgBI CAC'GTC
Cuts at: 0 123 723 2511
Size: 123 600 1788
Fragments arranged by size:
1788 600 123
BmrI ACTGGGnnnn_n'
Cuts at: 0 1044 1854 1944 2511
Size: 1044 810 90 567
Fragments arranged by size:
1044 810 567 90
BmtI G_CTAG'C
Cuts at: 0 8 2511
Size: 8 2503
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 693 1737 2319 2511
Size: 693 1044 582 192
Fragments arranged by size:
1044 693 582 192
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1069 1360 2451 2511
Size: 1069 291 1091 60
Fragments arranged by size:
1091 1069 291 60
Bpu10I CC'TnA_GC
Cuts at: 0 1271 1684 2511
Size: 1271 413 827
Fragments arranged by size:
1271 827 413
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 663 1345 2511
Size: 663 682 1166
Fragments arranged by size:
1166 682 663
BsaI GGTCTCn'nnnn_
Cuts at: 0 1212 1687 2511
Size: 1212 475 824
Fragments arranged by size:
1212 824 475
BsaAI yAC'GTr
Cuts at: 0 330 633 1562 2379 2511
Size: 330 303 929 817 132
Fragments arranged by size:
929 817 330 303 132
BsaWI w'CCGG_w
Cuts at: 0 1852 2309 2511
Size: 1852 457 202
Fragments arranged by size:
1852 457 202
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 370 400 1834 1864 2511
Size: 370 30 1434 30 647
Fragments arranged by size:
1434 647 370 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 625 691 922 994 1944 2371 2413 2511
Size: 625 66 231 72 950 427 42 98
Fragments arranged by size:
950 625 427 231 98 72 66 42
BseYI C'CCAG_C
Cuts at: 0 935 1879 2511
Size: 935 944 632
Fragments arranged by size:
944 935 632
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 356 2511
Size: 356 2155
BsiEI CG_ry'CG
Cuts at: 0 1557 2393 2511
Size: 1557 836 118
Fragments arranged by size:
1557 836 118
BsiHKAI G_wGCw'C
Cuts at: 0 701 914 1830 1936 2511
Size: 701 213 916 106 575
Fragments arranged by size:
916 701 575 213 106
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1167 2511
Size: 1167 1344
Bsp1286I G_dGCh'C
Cuts at: 0 71 599 701 767 914 1286 1818 1830
Size: 71 528 102 66 147 372 532 12
Cuts at: 1830 1860 1936 2158 2511
Size: 30 76 222 353
Fragments arranged by size:
532 528 372 353 222 147 102 76
71 66 30 12
BspHI T'CATG_A
Cuts at: 0 557 2511
Size: 557 1954
BsrBI CCG'CTC
Cuts at: 0 1311 2511
Size: 1311 1200
BsrFI r'CCGG_y
Cuts at: 0 1409 2309 2511
Size: 1409 900 202
Fragments arranged by size:
1409 900 202
BsrGI T'GTAC_A
Cuts at: 0 735 2511
Size: 735 1776
BstAPI GCAn_nnn'nTGC
Cuts at: 0 245 2511
Size: 245 2266
BstEII G'GTnAC_C
Cuts at: 0 1140 2235 2511
Size: 1140 1095 276
Fragments arranged by size:
1140 1095 276
BstYI r'GATC_y
Cuts at: 0 533 671 1106 2511
Size: 533 138 435 1405
Fragments arranged by size:
1405 533 435 138
Bsu36I CC'TnA_GG
Cuts at: 0 1927 2511
Size: 1927 584
BtgI C'CryG_G
Cuts at: 0 1187 1206 1781 2093 2144 2511
Size: 1187 19 575 312 51 367
Fragments arranged by size:
1187 575 367 312 51 19
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1251 1521 2511
Size: 1251 270 990
Fragments arranged by size:
1251 990 270
DrdI GACnn_nn'nnGTC
Cuts at: 0 1149 2511
Size: 1149 1362
EaeI y'GGCC_r
Cuts at: 0 125 1113 1209 1298 1532 1985 2147 2334
Size: 125 988 96 89 234 453 162 187
Cuts at: 2334 2502 2511
Size: 168 9
Fragments arranged by size:
988 453 234 187 168 162 125 96
89 9
EarI CTCTTCn'nnn_
Cuts at: 0 165 965 1210 2210 2511
Size: 165 800 245 1000 301
Fragments arranged by size:
1000 800 301 245 165
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1755 2511
Size: 1755 756
EcoICRI GAG'CTC
Cuts at: 0 699 2511
Size: 699 1812
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 442 1069 1360 2451 2511
Size: 442 627 291 1091 60
Fragments arranged by size:
1091 627 442 291 60
EcoO109I rG'GnC_Cy
Cuts at: 0 771 2196 2511
Size: 771 1425 315
Fragments arranged by size:
1425 771 315
EcoRV GAT'ATC
Cuts at: 0 1474 2511
Size: 1474 1037
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1239 1386 2511
Size: 1239 147 1125
Fragments arranged by size:
1239 1125 147
HaeII r_GCGC'y
Cuts at: 0 942 2511
Size: 942 1569
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 175 207 358 370 390 402 1341 1373
Size: 175 32 151 12 20 12 939 32
Cuts at: 1373 1834 1866 2395 2427 2511
Size: 461 32 529 32 84
Fragments arranged by size:
939 529 461 175 151 84 32 32
32 32 20 12 12
HincII GTy'rAC
Cuts at: 0 291 1154 1865 2511
Size: 291 863 711 646
Fragments arranged by size:
863 711 646 291
HindIII A'AGCT_T
Cuts at: 0 1744 2511
Size: 1744 767
HpaI GTT'AAC
Cuts at: 0 1865 2511
Size: 1865 646
MfeI C'AATT_G
Cuts at: 0 1895 2511
Size: 1895 616
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 401 2438 2504 2511
Size: 401 2037 66 7
Fragments arranged by size:
2037 401 66 7
MscI TGG'CCA
Cuts at: 0 127 1211 1300 1534 2504 2511
Size: 127 1084 89 234 970 7
Fragments arranged by size:
1084 970 234 127 89 7
MslI CAynn'nnrTG
Cuts at: 0 1036 1066 1420 2511
Size: 1036 30 354 1091
Fragments arranged by size:
1091 1036 354 30
MspA1I CmG'CkG
Cuts at: 0 667 741 889 959 1148 2511
Size: 667 74 148 70 189 1363
Fragments arranged by size:
1363 667 189 148 74 70
NcoI C'CATG_G
Cuts at: 0 1206 1781 2093 2144 2511
Size: 1206 575 312 51 367
Fragments arranged by size:
1206 575 367 312 51
NheI G'CTAG_C
Cuts at: 0 4 2511
Size: 4 2507
NsiI A_TGCA'T
Cuts at: 0 34 2511
Size: 34 2477
NspI r_CATG'y
Cuts at: 0 289 297 2511
Size: 289 8 2214
Fragments arranged by size:
2214 289 8
PacI TTA_AT'TAA
Cuts at: 0 431 2511
Size: 431 2080
PflMI CCAn_nnn'nTGG
Cuts at: 0 1809 2094 2511
Size: 1809 285 417
Fragments arranged by size:
1809 417 285
PmlI CAC'GTG
Cuts at: 0 633 2511
Size: 633 1878
PpuMI rG'GwC_Cy
Cuts at: 0 771 2196 2511
Size: 771 1425 315
Fragments arranged by size:
1425 771 315
PstI C_TGCA'G
Cuts at: 0 2496 2511
Size: 2496 15
PvuII CAG'CTG
Cuts at: 0 667 741 889 959 2511
Size: 667 74 148 70 1552
Fragments arranged by size:
1552 667 148 74 70
SacI G_AGCT'C
Cuts at: 0 701 2511
Size: 701 1810
SalI G'TCGA_C
Cuts at: 0 289 2511
Size: 289 2222
SfcI C'TryA_G
Cuts at: 0 2492 2511
Size: 2492 19
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 1540 2511
Size: 1540 971
SmaI CCC'GGG
Cuts at: 0 402 2511
Size: 402 2109
SmlI C'TyrA_G
Cuts at: 0 678 1360 2511
Size: 678 682 1151
Fragments arranged by size:
1151 682 678
SspI AAT'ATT
Cuts at: 0 2188 2511
Size: 2188 323
StyI C'CwwG_G
Cuts at: 0 128 222 767 1206 1223 1292 1781 2093
Size: 128 94 545 439 17 69 489 312
Cuts at: 2093 2144 2511
Size: 51 367
Fragments arranged by size:
545 489 439 367 312 128 94 69
51 17
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 917 949 1305 2511
Size: 917 32 356 1206
Fragments arranged by size:
1206 917 356 32
TatI w'GTAC_w
Cuts at: 0 735 2511
Size: 735 1776
Tth111I GACn'n_nGTC
Cuts at: 0 1553 2485 2511
Size: 1553 932 26
Fragments arranged by size:
1553 932 26
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1527 1995 2511
Size: 1527 468 516
Fragments arranged by size:
1527 516 468
XmaI C'CCGG_G
Cuts at: 0 400 2511
Size: 400 2111
XmnI GAAnn'nnTTC
Cuts at: 0 274 997 1456 1916 2511
Size: 274 723 459 460 595
Fragments arranged by size:
723 595 460 459 274
Enzymes that do cut:
AccI AcuI AfeI AflIII AgeI AleI AlwNI AvaI
BanI BanII BbsI BcgI BciVI BclI BfrBI BglI
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmBI Bsp1286I BspHI BsrBI BsrFI BsrGI
BstAPI BstEII BstYI Bsu36I BtgI BtgZI DrdI EaeI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRV FalI HaeII
Hin4I HincII HindIII HpaI MfeI MmeI MscI MslI
MspA1I NcoI NheI NsiI NspI PacI PflMI PmlI
PpuMI PstI PvuII SacI SalI SfcI SfiI SmaI
SmlI SspI StyI TaqII TatI Tth111I XcmI XmaI
XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AflII AhdI AlfI AloI
ApaI ApaLI ApoI AscI AseI AsiSI AvrII BaeI
BamHI BbeI BbvCI BglII BsaBI BsaHI BsiWI BsmI
BspEI BspMI BsrDI BssHII BssSI BstBI BstXI BstZ17I
BtsI ClaI CspCI DraI DraIII EagI EcoNI EcoRI
FseI FspI FspAI KasI KpnI MluI NaeI NarI
NdeI NgoMIV NotI NruI PasI PciI PfoI PmeI
PpiI PshAI PsiI PspOMI PspXI PsrI PvuI RsrII
SacII SanDI SapI SbfI ScaI SexAI SfoI SgfI
SgrAI SnaBI SpeI SphI SrfI StuI SwaI TstI
XbaI XhoI ZraI