Restriction map for pFN21ASDA0851
 (Circular) (Six-base) MAPSORT of: pFN21ASDA0851.seq  Check: 3652  from: 1  to: 6490



pFN21ASDA0851  6490 bp

 With 182 enzymes: SgfI * 

                        January 16, 2009 15:30  ..

AatII G_ACGT'C

Cuts at:    278     331     414     600    1536    4300     278 
   Size:         53      83     186     936    2764    2468

  Fragments arranged by size: 

                2764    2468     936     186      83      53
 
AccI GT'mk_AC

Cuts at:   1937    2200    3833    1937 
   Size:        263    1633    4594

  Fragments arranged by size: 

                4594    1633     263
 
Acc65I G'GTAC_C

Cuts at:   3811    3811 
   Size:       6490
 
AclI AA'CG_TT

Cuts at:   1563    4617    4990    1563 
   Size:       3054     373    3063

  Fragments arranged by size: 

                3063    3054     373
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1632    2069    2157    3230    3242    4553    5937    6205    1632 
   Size:        437      88    1073      12    1311    1384     268    1917

  Fragments arranged by size: 

                1917    1384    1311    1073     437     268      88      12
            
 
AflII C'TTAA_G

Cuts at:    820    1017     820 
   Size:        197    6293
 
AflIII A'CryG_T

Cuts at:   1203    5389    6366    1203 
   Size:       4186     977    1327

  Fragments arranged by size: 

                4186    1327     977
 
AgeI A'CCGG_T

Cuts at:   5321    5321 
   Size:       6490
 
AhdI GACnn_n'nnGTC

Cuts at:   2128    5219    2128 
   Size:       3091    3399
 
AleI CACnn'nnGTG

Cuts at:   1166    1166 
   Size:       6490
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1184    3000    1184 
   Size:       1816    4674
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   3158    3190    3464    3496    3158 
   Size:         32     274      32    6152

  Fragments arranged by size: 

                6152     274      32      32
 
AlwNI CAG_nnn'CTG

Cuts at:   4233    5805    4233 
   Size:       1572    4918
 
ApaLI G'TGCA_C

Cuts at:   2753    4547    5703    2753 
   Size:       1794    1156    3540

  Fragments arranged by size: 

                3540    1794    1156
 
ApoI r'AATT_y

Cuts at:   1493    2072    2270    2477    3308    3581    3799    4025    4177 
   Size:        579     198     207     831     273     218     226     152

Cuts at:   4177    6286    1493 
   Size:       2109    1697

  Fragments arranged by size: 

                2109    1697     831     579     273     226     218     207
                 198     152
 
AseI AT'TA_AT

Cuts at:    160    1032    2625    5044     160 
   Size:        872    1593    2419    1606

  Fragments arranged by size: 

                2419    1606    1593     872
 
AsiSI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       6490
 
AvaI C'yCGr_G

Cuts at:   1870    1943    2656    2790    3815    1870 
   Size:         73     713     134    1025    4545

  Fragments arranged by size: 

                4545    1025     713     134      73
 
BamHI G'GATC_C

Cuts at:   3820    4187    3820 
   Size:        367    6123
 
BanI G'GyrC_C

Cuts at:    618     943    1160    1787    3380    3811    5266     618 
   Size:        325     217     627    1593     431    1455    1842

  Fragments arranged by size: 

                1842    1593    1455     627     431     325     217
 
BanII G_rGCy'C

Cuts at:   1391    2960    3809    1391 
   Size:       1569     849    4072

  Fragments arranged by size: 

                4072    1569     849
 
BbsI GAAGACnn'nnnn_

Cuts at:    928    1899    2333    2991    3395     928 
   Size:        971     434     658     404    4023

  Fragments arranged by size: 

                4023     971     658     434     404
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1903    1937    3168    3202    4681    4715    1903 
   Size:         34    1231      34    1479      34    3678

  Fragments arranged by size: 

                3678    1479    1231      34      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:   4382    5592    4382 
   Size:       1210    5280
 
BclI T'GATC_A

Cuts at:   1548    1548 
   Size:       6490
 
BfrBI ATG'CAT

Cuts at:   2611    2611 
   Size:       6490
 
BglI GCCn_nnn'nGGC

Cuts at:    136     243     365     436    1067    1672    5101     136 
   Size:        107     122      71     631     605    3429    1525

  Fragments arranged by size: 

                3429    1525     631     605     122     107      71
 
BglII A'GATC_T

Cuts at:   6485    6485 
   Size:       6490
 
BlpI GC'TnA_GC

Cuts at:   3907    3907 
   Size:       6490
 
Bme1580I G_kGCm'C

Cuts at:   1790    2210    2757    4551    5707    1790 
   Size:        420     547    1794    1156    2573

  Fragments arranged by size: 

                2573    1794    1156     547     420
 
BmgBI CAC'GTC

Cuts at:   1318    1318 
   Size:       6490
 
BmrI ACTGGGnnnn_n'

Cuts at:    442     858     911     994    1393    1417    2213    4221    5179 
   Size:        416      53      83     399      24     796    2008     958

Cuts at:   5179     442 
   Size:       1753

  Fragments arranged by size: 

                2008    1753     958     796     416     399      83      53
                  24
 
BmtI G_CTAG'C

Cuts at:   1056    1056 
   Size:       6490
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    5150    1239 
   Size:       3911    2579
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   2816    2993    4615    5480    5778    2816 
   Size:        177    1622     865     298    3528

  Fragments arranged by size: 

                3528    1622     865     298     177
 
BsaI GGTCTCn'nnnn_

Cuts at:    882    1499    5153     882 
   Size:        617    3654    2219

  Fragments arranged by size: 

                3654    2219     617
 
BsaAI yAC'GTr

Cuts at:    493    1204    3787    4281     493 
   Size:        711    2583     494    2702

  Fragments arranged by size: 

                2702    2583     711     494
 
BsaBI GATnn'nnATC

Cuts at:   2651    6484    2651 
   Size:       3833    2657
 
BsaHI Gr'CG_yC

Cuts at:    275     328     411     597    1533    4297    4679    5314     275 
   Size:         53      83     186     936    2764     382     635    1451

  Fragments arranged by size: 

                2764    1451     936     635     382     186      83      53
            
 
BsaWI w'CCGG_w

Cuts at:   4923    5321    5595    5742    6153    4923 
   Size:        398     274     147     411    5260

  Fragments arranged by size: 

                5260     411     398     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1178    1208    1373    1403    2653    2683    1178 
   Size:         30     165      30    1250      30    4985

  Fragments arranged by size: 

                4985    1250     165      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1186    2674    2677    1186 
   Size:       1488       3    4999

  Fragments arranged by size: 

                4999    1488       3
 
BseYI C'CCAG_C

Cuts at:   5693    6189    5693 
   Size:        496    5994
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1163    1736    2538    1163 
   Size:        573     802    5115

  Fragments arranged by size: 

                5115     802     573
 
BsiEI CG_ry'CG

Cuts at:   1540    1815    2001    3940    4701    5729    1540 
   Size:        275     186    1939     761    1028    2301

  Fragments arranged by size: 

                2301    1939    1028     761     275     186
 
BsiHKAI G_wGCw'C

Cuts at:   2757    3684    4551    4636    5707    2757 
   Size:        927     867      85    1071    3540

  Fragments arranged by size: 

                3540    1071     927     867      85
 
BsmI GAATG_Cn'

Cuts at:   2611    4001    4094    2611 
   Size:       1390      93    5007

  Fragments arranged by size: 

                5007    1390      93
 
Bsp1286I G_dGCh'C

Cuts at:   1391    1790    2210    2757    2960    3684    3809    4551    4636 
   Size:        399     420     547     203     724     125     742      85

Cuts at:   4636    5707    1391 
   Size:       1071    2174

  Fragments arranged by size: 

                2174    1071     742     724     547     420     399     203
                 125      85
 
BspEI T'CCGG_A

Cuts at:   6153    6153 
   Size:       6490
 
BspHI T'CATG_A

Cuts at:   4379    6109    4379 
   Size:       1730    4760
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    844    2216    3845     844 
   Size:       1372    1629    3489

  Fragments arranged by size: 

                3489    1629    1372
 
BsrBI CCG'CTC

Cuts at:   1394    1823    4377    1394 
   Size:        429    2554    3507

  Fragments arranged by size: 

                3507    2554     429
 
BsrDI GCAATG_nn'

Cuts at:     65    1248    3027    4985    5159      65 
   Size:       1183    1779    1958     174    1396

  Fragments arranged by size: 

                1958    1779    1396    1183     174
 
BsrFI r'CCGG_y

Cuts at:   1700    1810    5134    5321    1700 
   Size:        110    3324     187    2869

  Fragments arranged by size: 

                3324    2869     187     110
 
BsrGI T'GTAC_A

Cuts at:     96    2782      96 
   Size:       2686    3804
 
BssHII G'CGCG_C

Cuts at:   1925    1925 
   Size:       6490
 
BssSI C'ACGA_G

Cuts at:   4550    5562    4550 
   Size:       1012    5478
 
BstAPI GCAn_nnn'nTGC

Cuts at:    794     794 
   Size:       6490
 
BstBI TT'CG_AA

Cuts at:   2007    5294    2007 
   Size:       3287    3203
 
BstEII G'GTnAC_C

Cuts at:   1184    2140    1184 
   Size:        956    5534
 
BstXI CCAn_nnnn'nTGG

Cuts at:   3576    3576 
   Size:       6490
 
BstYI r'GATC_y

Cuts at:   1972    3820    4187    4572    4589    6041    6127    6139    6485 
   Size:       1848     367     385      17    1452      86      12     346

Cuts at:   6485    1972 
   Size:       1977

  Fragments arranged by size: 

                1977    1848    1452     385     367     346      86      17
                  12
 
BstZ17I GTA'TAC

Cuts at:   2201    2201 
   Size:       6490
 
BtgI C'CryG_G

Cuts at:    513    1065    2022     513 
   Size:        552     957    4981

  Fragments arranged by size: 

                4981     957     552
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    487    1170    1226    2396     487 
   Size:        683      56    1170    4581

  Fragments arranged by size: 

                4581    1170     683      56
 
BtsI GCAGTG_nn'

Cuts at:   2843    4006    4800    4820    2843 
   Size:       1163     794      20    4513

  Fragments arranged by size: 

                4513    1163     794      20
 
ClaI AT'CG_AT

Cuts at:   1554    1554 
   Size:       6490
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    529     564     529 
   Size:         35    6455
 
DraI TTT'AAA

Cuts at:   3794    3794 
   Size:       6490
 
DrdI GACnn_nn'nnGTC

Cuts at:    809    1316    5497     809 
   Size:        507    4181    1802

  Fragments arranged by size: 

                4181    1802     507
 
EaeI y'GGCC_r

Cuts at:      8      62    1487    1537    1812    1830    3145    3937    4826 
   Size:         54    1425      50     275      18    1315     792     889

Cuts at:   4826    6455       8 
   Size:       1629      43

  Fragments arranged by size: 

                1629    1425    1315     889     792     275      54      50
                  43      18
 
EagI C'GGCC_G

Cuts at:   1537    1812    3937    1537 
   Size:        275    2125    4090

  Fragments arranged by size: 

                4090    2125     275
 
EarI CTCTTCn'nnn_

Cuts at:   1353    3689    4420    1353 
   Size:       2336     731    3423

  Fragments arranged by size: 

                3423    2336     731
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    387    1451    1589    5077    5451    5597     387 
   Size:       1064     138    3488     374     146    1280

  Fragments arranged by size: 

                3488    1280    1064     374     146     138
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    1632    2069    2157    3230    3242    4553    5150    5937 
   Size:        393     437      88    1073      12    1311     597     787

Cuts at:   5937    6205    1239 
   Size:        268    1524

  Fragments arranged by size: 

                1524    1311    1073     787     597     437     393     268
                  88      12
 
EcoRI G'AATT_C

Cuts at:   2477    3799    4177    2477 
   Size:       1322     378    4790

  Fragments arranged by size: 

                4790    1322     378
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   3412    3493    3412 
   Size:         81    6409
 
FspI TGC'GCA

Cuts at:   4996    4996 
   Size:       6490
 
HaeII r_GCGC'y

Cuts at:   1678    5637    1678 
   Size:       3959    2531
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1373    1405    1540    1572    2114    2146    2814    2846    3055 
   Size:         32     135      32     542      32     668      32     209

Cuts at:   3055    3087    5131    5163    5205    5237    1373 
   Size:         32    2044      32      42      32    2626

  Fragments arranged by size: 

                2626    2044     668     542     209     135      42      32
                  32      32      32      32      32      32
 
HincII GTy'rAC

Cuts at:    669    1657    1938    3834    4080     669 
   Size:        988     281    1896     246    3079

  Fragments arranged by size: 

                3079    1896     988     281     246
 
HindIII A'AGCT_T

Cuts at:    748    2085    2772    3290     748 
   Size:       1337     687     518    3948

  Fragments arranged by size: 

                3948    1337     687     518
 
HpaI GTT'AAC

Cuts at:   4080    4080 
   Size:       6490
 
KpnI G_GTAC'C

Cuts at:   3815    3815 
   Size:       6490
 
MfeI C'AATT_G

Cuts at:   3184    4089    3184 
   Size:        905    5585
 
MluI A'CGCG_T

Cuts at:   6366    6366 
   Size:       6490
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1305    2487    5604    5788    1305 
   Size:       1182    3117     184    2007

  Fragments arranged by size: 

                3117    2007    1182     184
 
MscI TGG'CCA

Cuts at:     10      64    1489    1832    3147      10 
   Size:         54    1425     343    1315    3353

  Fragments arranged by size: 

                3353    1425    1315     343      54
 
MslI CAynn'nnrTG

Cuts at:    518    1166    2760    4448    4807    4966     518 
   Size:        648    1594    1688     359     159    2042

  Fragments arranged by size: 

                2042    1688    1594     648     359     159
 
MspA1I CmG'CkG

Cuts at:   1606    2047    2177    2455    2515    3906    4583    5731    5976 
   Size:        441     130     278      60    1391     677    1148     245

Cuts at:   5976    1606 
   Size:       2120

  Fragments arranged by size: 

                2120    1391    1148     677     441     278     245     130
                  60
 
NcoI C'CATG_G

Cuts at:    513    1065    2022     513 
   Size:        552     957    4981

  Fragments arranged by size: 

                4981     957     552
 
NdeI CA'TA_TG

Cuts at:    387    2431     387 
   Size:       2044    4446
 
NheI G'CTAG_C

Cuts at:   1052    1052 
   Size:       6490
 
NotI GC'GGCC_GC

Cuts at:   3937    3937 
   Size:       6490
 
NsiI A_TGCA'T

Cuts at:   2613    2613 
   Size:       6490
 
NspI r_CATG'y

Cuts at:   1132    1229    3281    3848    5393    1132 
   Size:         97    2052     567    1545    2229

  Fragments arranged by size: 

                2229    2052    1545     567      97
 
PasI CC'CwG_GG

Cuts at:   1348    1348 
   Size:       6490
 
PciI A'CATG_T

Cuts at:   5389    5389 
   Size:       6490
 
PflMI CCAn_nnn'nTGG

Cuts at:   1108    2195    2221    3154    1108 
   Size:       1087      26     933    4444

  Fragments arranged by size: 

                4444    1087     933      26
 
PmeI GTTT'AAAC

Cuts at:   3794    3794 
   Size:       6490
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   4559    4591    6072    6104    4559 
   Size:         32    1481      32    4945

  Fragments arranged by size: 

                4945    1481      32      32
 
PshAI GACnn'nnGTC

Cuts at:   1613    1613 
   Size:       6490
 
PsiI TTA'TAA

Cuts at:   2322    2933    3434    3468    4060    2322 
   Size:        611     501      34     592    4752

  Fragments arranged by size: 

                4752     611     592     501      34
 
PspXI vC'TCGA_Gb

Cuts at:   2790    2790 
   Size:       6490
 
PstI C_TGCA'G

Cuts at:    830    3842     830 
   Size:       3012    3478
 
PvuI CG_AT'CG

Cuts at:   2001    2001 
   Size:       6490
 
PvuII CAG'CTG

Cuts at:   2047    2177    2047 
   Size:        130    6360
 
SalI G'TCGA_C

Cuts at:   1936    3832    1936 
   Size:       1896    4594
 
SapI GCTCTTCn'nnn_

Cuts at:   3689    3689 
   Size:       6490
 
SbfI CC_TGCA'GG

Cuts at:   3842    3842 
   Size:       6490
 
ScaI AGT'ACT

Cuts at:   4738    6450    4738 
   Size:       1712    4778
 
SfcI C'TryA_G

Cuts at:    826    1045    3838    4973    5654    5845     826 
   Size:        219    2793    1135     681     191    1471

  Fragments arranged by size: 

                2793    1471    1135     681     219     191
 
SgfI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       6490
 
SgrAI Cr'CCGG_yG

Cuts at:   1700    1700 
   Size:       6490
 
SmaI CCC'GGG

Cuts at:   1872    3817    1872 
   Size:       1945    4545
 
SmlI C'TyrA_G

Cuts at:    820    1017    1943    2656    2790    2831    2972    4594    5495 
   Size:        197     926     713     134      41     141    1622     901

Cuts at:   5495    5757     820 
   Size:        262    1553

  Fragments arranged by size: 

                1622    1553     926     901     713     262     197     141
                 134      41
 
SnaBI TAC'GTA

Cuts at:    493    3787    4281     493 
   Size:       3294     494    2702

  Fragments arranged by size: 

                3294    2702     494
 
SpeI A'CTAG_T

Cuts at:    152    2468     152 
   Size:       2316    4174
 
SphI G_CATG'C

Cuts at:   1132    3848    1132 
   Size:       2716    3774
 
SspI AAT'ATT

Cuts at:      5      52    2193    4414       5 
   Size:         47    2141    2221    2081

  Fragments arranged by size: 

                2221    2141    2081      47
 
StuI AGG'CCT

Cuts at:   1517    1517 
   Size:       6490
 
StyI C'CwwG_G

Cuts at:    513    1065    2022    3003    3569    3929     513 
   Size:        552     957     981     566     360    3074

  Fragments arranged by size: 

                3074     981     957     566     552     360
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    325    1580    4517    4534    4687    4872    5317     325 
   Size:       1255    2937      17     153     185     445    1498

  Fragments arranged by size: 

                2937    1498    1255     445     185     153      17
 
TatI w'GTAC_w

Cuts at:     96     370     450     483     534    1977    2782    2835    4736 
   Size:        274      80      33      51    1443     805      53    1901

Cuts at:   4736    6448      96 
   Size:       1712     138

  Fragments arranged by size: 

                1901    1712    1443     805     274     138      80      53
                  51      33
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   6450    6482    6450 
   Size:         32    6458
 
Tth111I GACn'n_nGTC

Cuts at:   1316    1316 
   Size:       6490
 
XbaI T'CTAG_A

Cuts at:   3199    3826    3199 
   Size:        627    5863
 
XhoI C'TCGA_G

Cuts at:   1943    2656    2790    1943 
   Size:        713     134    5643

  Fragments arranged by size: 

                5643     713     134
 
XmaI C'CCGG_G

Cuts at:   1870    3815    1870 
   Size:       1945    4545
 
XmnI GAAnn'nnTTC

Cuts at:   2707    3526    4619    2707 
   Size:        819    1093    4578

  Fragments arranged by size: 

                4578    1093     819
 
ZraI GAC'GTC

Cuts at:    276     329     412     598    1534    4298     276 
   Size:         53      83     186     936    2764    2468

  Fragments arranged by size: 

                2764    2468     936     186      83      53
 

 Enzymes that do cut:

    AatII     AccI   Acc65I     AclI     AcuI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI    AlwNI    ApaLI     ApoI     AseI
    AsiSI     AvaI    BamHI     BanI    BanII     BbsI     BcgI    BciVI
     BclI    BfrBI     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI
     BmtI     BpmI    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI
    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI Bsp1286I
    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII
    BssSI   BstAPI    BstBI   BstEII    BstXI    BstYI  BstZ17I     BtgI
    BtgZI     BtsI     ClaI    CspCI     DraI     DrdI     EaeI     EagI
     EarI     EciI  Eco57MI    EcoRI     FalI     FspI    HaeII    Hin4I
   HincII  HindIII     HpaI     KpnI     MfeI     MluI     MmeI     MscI
     MslI   MspA1I     NcoI     NdeI     NheI     NotI     NsiI     NspI
     PasI     PciI    PflMI     PmeI     PpiI    PshAI     PsiI    PspXI
     PstI     PvuI    PvuII     SalI     SapI     SbfI     ScaI     SfcI
     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SspI
     StuI     StyI    TaqII     TatI     TstI  Tth111I     XbaI     XhoI
     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

     AarI     AfeI     ApaI     AscI    AvrII     BaeI     BbeI    BbvCI
     BplI   Bpu10I    BsiWI    BsmBI   Bsu36I   DraIII  EcoICRI    EcoNI
 EcoO109I    EcoRV     FseI    FspAI     KasI     NaeI     NarI   NgoMIV
     NruI     PacI     PfoI     PmlI    PpuMI   PspOMI     PsrI    RsrII
     SacI    SacII    SanDI    SexAI     SfiI     SfoI     SrfI     SwaI
     XcmI
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