(Circular) (Six-base) MAPSORT of: pFN21ASDA0851.seq Check: 3652 from: 1 to: 6490 pFN21ASDA0851 6490 bp With 182 enzymes: SgfI * January 16, 2009 15:30 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4300 278 Size: 53 83 186 936 2764 2468 Fragments arranged by size: 2764 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2200 3833 1937 Size: 263 1633 4594 Fragments arranged by size: 4594 1633 263 Acc65I G'GTAC_C Cuts at: 3811 3811 Size: 6490 AclI AA'CG_TT Cuts at: 1563 4617 4990 1563 Size: 3054 373 3063 Fragments arranged by size: 3063 3054 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2069 2157 3230 3242 4553 5937 6205 1632 Size: 437 88 1073 12 1311 1384 268 1917 Fragments arranged by size: 1917 1384 1311 1073 437 268 88 12 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6293 AflIII A'CryG_T Cuts at: 1203 5389 6366 1203 Size: 4186 977 1327 Fragments arranged by size: 4186 1327 977 AgeI A'CCGG_T Cuts at: 5321 5321 Size: 6490 AhdI GACnn_n'nnGTC Cuts at: 2128 5219 2128 Size: 3091 3399 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6490 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3000 1184 Size: 1816 4674 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3158 3190 3464 3496 3158 Size: 32 274 32 6152 Fragments arranged by size: 6152 274 32 32 AlwNI CAG_nnn'CTG Cuts at: 4233 5805 4233 Size: 1572 4918 ApaLI G'TGCA_C Cuts at: 2753 4547 5703 2753 Size: 1794 1156 3540 Fragments arranged by size: 3540 1794 1156 ApoI r'AATT_y Cuts at: 1493 2072 2270 2477 3308 3581 3799 4025 4177 Size: 579 198 207 831 273 218 226 152 Cuts at: 4177 6286 1493 Size: 2109 1697 Fragments arranged by size: 2109 1697 831 579 273 226 218 207 198 152 AseI AT'TA_AT Cuts at: 160 1032 2625 5044 160 Size: 872 1593 2419 1606 Fragments arranged by size: 2419 1606 1593 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6490 AvaI C'yCGr_G Cuts at: 1870 1943 2656 2790 3815 1870 Size: 73 713 134 1025 4545 Fragments arranged by size: 4545 1025 713 134 73 BamHI G'GATC_C Cuts at: 3820 4187 3820 Size: 367 6123 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3380 3811 5266 618 Size: 325 217 627 1593 431 1455 1842 Fragments arranged by size: 1842 1593 1455 627 431 325 217 BanII G_rGCy'C Cuts at: 1391 2960 3809 1391 Size: 1569 849 4072 Fragments arranged by size: 4072 1569 849 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2333 2991 3395 928 Size: 971 434 658 404 4023 Fragments arranged by size: 4023 971 658 434 404 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3168 3202 4681 4715 1903 Size: 34 1231 34 1479 34 3678 Fragments arranged by size: 3678 1479 1231 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4382 5592 4382 Size: 1210 5280 BclI T'GATC_A Cuts at: 1548 1548 Size: 6490 BfrBI ATG'CAT Cuts at: 2611 2611 Size: 6490 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5101 136 Size: 107 122 71 631 605 3429 1525 Fragments arranged by size: 3429 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6485 6485 Size: 6490 BlpI GC'TnA_GC Cuts at: 3907 3907 Size: 6490 Bme1580I G_kGCm'C Cuts at: 1790 2210 2757 4551 5707 1790 Size: 420 547 1794 1156 2573 Fragments arranged by size: 2573 1794 1156 547 420 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6490 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2213 4221 5179 Size: 416 53 83 399 24 796 2008 958 Cuts at: 5179 442 Size: 1753 Fragments arranged by size: 2008 1753 958 796 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6490 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 5150 1239 Size: 3911 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2816 2993 4615 5480 5778 2816 Size: 177 1622 865 298 3528 Fragments arranged by size: 3528 1622 865 298 177 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5153 882 Size: 617 3654 2219 Fragments arranged by size: 3654 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3787 4281 493 Size: 711 2583 494 2702 Fragments arranged by size: 2702 2583 711 494 BsaBI GATnn'nnATC Cuts at: 2651 6484 2651 Size: 3833 2657 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4297 4679 5314 275 Size: 53 83 186 936 2764 382 635 1451 Fragments arranged by size: 2764 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4923 5321 5595 5742 6153 4923 Size: 398 274 147 411 5260 Fragments arranged by size: 5260 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2653 2683 1178 Size: 30 165 30 1250 30 4985 Fragments arranged by size: 4985 1250 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2674 2677 1186 Size: 1488 3 4999 Fragments arranged by size: 4999 1488 3 BseYI C'CCAG_C Cuts at: 5693 6189 5693 Size: 496 5994 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2538 1163 Size: 573 802 5115 Fragments arranged by size: 5115 802 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3940 4701 5729 1540 Size: 275 186 1939 761 1028 2301 Fragments arranged by size: 2301 1939 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2757 3684 4551 4636 5707 2757 Size: 927 867 85 1071 3540 Fragments arranged by size: 3540 1071 927 867 85 BsmI GAATG_Cn' Cuts at: 2611 4001 4094 2611 Size: 1390 93 5007 Fragments arranged by size: 5007 1390 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2210 2757 2960 3684 3809 4551 4636 Size: 399 420 547 203 724 125 742 85 Cuts at: 4636 5707 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 724 547 420 399 203 125 85 BspEI T'CCGG_A Cuts at: 6153 6153 Size: 6490 BspHI T'CATG_A Cuts at: 4379 6109 4379 Size: 1730 4760 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2216 3845 844 Size: 1372 1629 3489 Fragments arranged by size: 3489 1629 1372 BsrBI CCG'CTC Cuts at: 1394 1823 4377 1394 Size: 429 2554 3507 Fragments arranged by size: 3507 2554 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3027 4985 5159 65 Size: 1183 1779 1958 174 1396 Fragments arranged by size: 1958 1779 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5134 5321 1700 Size: 110 3324 187 2869 Fragments arranged by size: 3324 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2782 96 Size: 2686 3804 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6490 BssSI C'ACGA_G Cuts at: 4550 5562 4550 Size: 1012 5478 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6490 BstBI TT'CG_AA Cuts at: 2007 5294 2007 Size: 3287 3203 BstEII G'GTnAC_C Cuts at: 1184 2140 1184 Size: 956 5534 BstXI CCAn_nnnn'nTGG Cuts at: 3576 3576 Size: 6490 BstYI r'GATC_y Cuts at: 1972 3820 4187 4572 4589 6041 6127 6139 6485 Size: 1848 367 385 17 1452 86 12 346 Cuts at: 6485 1972 Size: 1977 Fragments arranged by size: 1977 1848 1452 385 367 346 86 17 12 BstZ17I GTA'TAC Cuts at: 2201 2201 Size: 6490 BtgI C'CryG_G Cuts at: 513 1065 2022 513 Size: 552 957 4981 Fragments arranged by size: 4981 957 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2396 487 Size: 683 56 1170 4581 Fragments arranged by size: 4581 1170 683 56 BtsI GCAGTG_nn' Cuts at: 2843 4006 4800 4820 2843 Size: 1163 794 20 4513 Fragments arranged by size: 4513 1163 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6490 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6455 DraI TTT'AAA Cuts at: 3794 3794 Size: 6490 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5497 809 Size: 507 4181 1802 Fragments arranged by size: 4181 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3145 3937 4826 Size: 54 1425 50 275 18 1315 792 889 Cuts at: 4826 6455 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1315 889 792 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3937 1537 Size: 275 2125 4090 Fragments arranged by size: 4090 2125 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3689 4420 1353 Size: 2336 731 3423 Fragments arranged by size: 3423 2336 731 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5077 5451 5597 387 Size: 1064 138 3488 374 146 1280 Fragments arranged by size: 3488 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2069 2157 3230 3242 4553 5150 5937 Size: 393 437 88 1073 12 1311 597 787 Cuts at: 5937 6205 1239 Size: 268 1524 Fragments arranged by size: 1524 1311 1073 787 597 437 393 268 88 12 EcoRI G'AATT_C Cuts at: 2477 3799 4177 2477 Size: 1322 378 4790 Fragments arranged by size: 4790 1322 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3412 3493 3412 Size: 81 6409 FspI TGC'GCA Cuts at: 4996 4996 Size: 6490 HaeII r_GCGC'y Cuts at: 1678 5637 1678 Size: 3959 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2114 2146 2814 2846 3055 Size: 32 135 32 542 32 668 32 209 Cuts at: 3055 3087 5131 5163 5205 5237 1373 Size: 32 2044 32 42 32 2626 Fragments arranged by size: 2626 2044 668 542 209 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3834 4080 669 Size: 988 281 1896 246 3079 Fragments arranged by size: 3079 1896 988 281 246 HindIII A'AGCT_T Cuts at: 748 2085 2772 3290 748 Size: 1337 687 518 3948 Fragments arranged by size: 3948 1337 687 518 HpaI GTT'AAC Cuts at: 4080 4080 Size: 6490 KpnI G_GTAC'C Cuts at: 3815 3815 Size: 6490 MfeI C'AATT_G Cuts at: 3184 4089 3184 Size: 905 5585 MluI A'CGCG_T Cuts at: 6366 6366 Size: 6490 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2487 5604 5788 1305 Size: 1182 3117 184 2007 Fragments arranged by size: 3117 2007 1182 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3147 10 Size: 54 1425 343 1315 3353 Fragments arranged by size: 3353 1425 1315 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2760 4448 4807 4966 518 Size: 648 1594 1688 359 159 2042 Fragments arranged by size: 2042 1688 1594 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2047 2177 2455 2515 3906 4583 5731 5976 Size: 441 130 278 60 1391 677 1148 245 Cuts at: 5976 1606 Size: 2120 Fragments arranged by size: 2120 1391 1148 677 441 278 245 130 60 NcoI C'CATG_G Cuts at: 513 1065 2022 513 Size: 552 957 4981 Fragments arranged by size: 4981 957 552 NdeI CA'TA_TG Cuts at: 387 2431 387 Size: 2044 4446 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6490 NotI GC'GGCC_GC Cuts at: 3937 3937 Size: 6490 NsiI A_TGCA'T Cuts at: 2613 2613 Size: 6490 NspI r_CATG'y Cuts at: 1132 1229 3281 3848 5393 1132 Size: 97 2052 567 1545 2229 Fragments arranged by size: 2229 2052 1545 567 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6490 PciI A'CATG_T Cuts at: 5389 5389 Size: 6490 PflMI CCAn_nnn'nTGG Cuts at: 1108 2195 2221 3154 1108 Size: 1087 26 933 4444 Fragments arranged by size: 4444 1087 933 26 PmeI GTTT'AAAC Cuts at: 3794 3794 Size: 6490 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4559 4591 6072 6104 4559 Size: 32 1481 32 4945 Fragments arranged by size: 4945 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6490 PsiI TTA'TAA Cuts at: 2322 2933 3434 3468 4060 2322 Size: 611 501 34 592 4752 Fragments arranged by size: 4752 611 592 501 34 PspXI vC'TCGA_Gb Cuts at: 2790 2790 Size: 6490 PstI C_TGCA'G Cuts at: 830 3842 830 Size: 3012 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6490 PvuII CAG'CTG Cuts at: 2047 2177 2047 Size: 130 6360 SalI G'TCGA_C Cuts at: 1936 3832 1936 Size: 1896 4594 SapI GCTCTTCn'nnn_ Cuts at: 3689 3689 Size: 6490 SbfI CC_TGCA'GG Cuts at: 3842 3842 Size: 6490 ScaI AGT'ACT Cuts at: 4738 6450 4738 Size: 1712 4778 SfcI C'TryA_G Cuts at: 826 1045 3838 4973 5654 5845 826 Size: 219 2793 1135 681 191 1471 Fragments arranged by size: 2793 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6490 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6490 SmaI CCC'GGG Cuts at: 1872 3817 1872 Size: 1945 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2656 2790 2831 2972 4594 5495 Size: 197 926 713 134 41 141 1622 901 Cuts at: 5495 5757 820 Size: 262 1553 Fragments arranged by size: 1622 1553 926 901 713 262 197 141 134 41 SnaBI TAC'GTA Cuts at: 493 3787 4281 493 Size: 3294 494 2702 Fragments arranged by size: 3294 2702 494 SpeI A'CTAG_T Cuts at: 152 2468 152 Size: 2316 4174 SphI G_CATG'C Cuts at: 1132 3848 1132 Size: 2716 3774 SspI AAT'ATT Cuts at: 5 52 2193 4414 5 Size: 47 2141 2221 2081 Fragments arranged by size: 2221 2141 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6490 StyI C'CwwG_G Cuts at: 513 1065 2022 3003 3569 3929 513 Size: 552 957 981 566 360 3074 Fragments arranged by size: 3074 981 957 566 552 360 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4517 4534 4687 4872 5317 325 Size: 1255 2937 17 153 185 445 1498 Fragments arranged by size: 2937 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2782 2835 4736 Size: 274 80 33 51 1443 805 53 1901 Cuts at: 4736 6448 96 Size: 1712 138 Fragments arranged by size: 1901 1712 1443 805 274 138 80 53 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6450 6482 6450 Size: 32 6458 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6490 XbaI T'CTAG_A Cuts at: 3199 3826 3199 Size: 627 5863 XhoI C'TCGA_G Cuts at: 1943 2656 2790 1943 Size: 713 134 5643 Fragments arranged by size: 5643 713 134 XmaI C'CCGG_G Cuts at: 1870 3815 1870 Size: 1945 4545 XmnI GAAnn'nnTTC Cuts at: 2707 3526 4619 2707 Size: 819 1093 4578 Fragments arranged by size: 4578 1093 819 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4298 276 Size: 53 83 186 936 2764 2468 Fragments arranged by size: 2764 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspXI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI BplI Bpu10I BsiWI BsmBI Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PpuMI PspOMI PsrI RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI