(Circular) (Six-base) MAPSORT of: pFN21ASDA0833.seq Check: 2512 from: 1 to: 9748 pFN21ASDA0833 9748 bp With 182 enzymes: SgfI * January 16, 2009 15:30 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 5526 5526 Size: 9748 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 7558 278 Size: 53 83 186 936 6022 2468 Fragments arranged by size: 6022 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 6014 7091 1937 Size: 4077 1077 4594 Fragments arranged by size: 4594 4077 1077 Acc65I G'GTAC_C Cuts at: 7069 7069 Size: 9748 AclI AA'CG_TT Cuts at: 1563 7875 8248 1563 Size: 6312 373 3063 Fragments arranged by size: 6312 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2513 3571 3763 3802 4312 5893 7811 9195 Size: 881 1058 192 39 510 1581 1918 1384 Cuts at: 9195 9463 1632 Size: 268 1917 Fragments arranged by size: 1918 1917 1581 1384 1058 881 510 268 192 39 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 9551 AflIII A'CryG_T Cuts at: 1203 2943 3399 3807 8647 9624 1203 Size: 1740 456 408 4840 977 1327 Fragments arranged by size: 4840 1740 1327 977 456 408 AgeI A'CCGG_T Cuts at: 8579 8579 Size: 9748 AhdI GACnn_n'nnGTC Cuts at: 3808 4924 8477 3808 Size: 1116 3553 5079 Fragments arranged by size: 5079 3553 1116 AleI CACnn'nnGTG Cuts at: 1166 3161 3398 3947 1166 Size: 1995 237 549 6967 Fragments arranged by size: 6967 1995 549 237 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 4259 4495 1184 Size: 3075 236 6437 Fragments arranged by size: 6437 3075 236 AlwNI CAG_nnn'CTG Cuts at: 3993 4757 5942 6311 7491 9063 3993 Size: 764 1185 369 1180 1572 4678 Fragments arranged by size: 4678 1572 1185 1180 764 369 ApaI G_GGCC'C Cuts at: 4459 4459 Size: 9748 ApaLI G'TGCA_C Cuts at: 2552 7805 8961 2552 Size: 5253 1156 3339 Fragments arranged by size: 5253 3339 1156 ApoI r'AATT_y Cuts at: 1493 5986 6268 6512 6761 6788 7057 7283 7435 Size: 4493 282 244 249 27 269 226 152 Cuts at: 7435 9544 1493 Size: 2109 1697 Fragments arranged by size: 4493 2109 1697 282 269 249 244 226 152 27 AseI AT'TA_AT Cuts at: 160 1032 8302 160 Size: 872 7270 1606 Fragments arranged by size: 7270 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 9748 AvaI C'yCGr_G Cuts at: 1870 1943 3572 3862 4231 4884 5873 7073 1870 Size: 73 1629 290 369 653 989 1200 4545 Fragments arranged by size: 4545 1629 1200 989 653 369 290 73 AvrII C'CTAG_G Cuts at: 4603 5380 4603 Size: 777 8971 BamHI G'GATC_C Cuts at: 7078 7445 7078 Size: 367 9381 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2235 3941 4398 4598 7069 Size: 325 217 627 448 1706 457 200 2471 Cuts at: 7069 8524 618 Size: 1455 1842 Fragments arranged by size: 2471 1842 1706 1455 627 457 448 325 217 200 BanII G_rGCy'C Cuts at: 1391 2664 2984 3095 3120 3332 4302 4459 4891 Size: 1273 320 111 25 212 970 157 432 Cuts at: 4891 5586 6443 7067 1391 Size: 695 857 624 4072 Fragments arranged by size: 4072 1273 970 857 695 624 432 320 212 157 111 25 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 5695 928 Size: 971 3796 4981 Fragments arranged by size: 4981 3796 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2173 2207 7939 7973 1903 Size: 34 236 34 5732 34 3678 Fragments arranged by size: 5732 3678 236 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 7640 8850 7640 Size: 1210 8538 BclI T'GATC_A Cuts at: 1548 2140 4350 6574 1548 Size: 592 2210 2224 4722 Fragments arranged by size: 4722 2224 2210 592 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3828 4076 4589 Size: 107 122 71 631 605 2156 248 513 Cuts at: 4589 5174 6447 8359 136 Size: 585 1273 1912 1525 Fragments arranged by size: 2156 1912 1525 1273 631 605 585 513 248 122 107 71 BglII A'GATC_T Cuts at: 4746 5995 9743 4746 Size: 1249 3748 4751 Fragments arranged by size: 4751 3748 1249 BlpI GC'TnA_GC Cuts at: 4301 4520 5538 6558 7165 4301 Size: 219 1018 1020 607 6884 Fragments arranged by size: 6884 1020 1018 607 219 Bme1580I G_kGCm'C Cuts at: 1790 2556 3944 4401 4459 4601 5380 5388 6279 Size: 766 1388 457 58 142 779 8 891 Cuts at: 6279 7809 8965 1790 Size: 1530 1156 2573 Fragments arranged by size: 2573 1530 1388 1156 891 779 766 457 142 58 8 BmgBI CAC'GTC Cuts at: 1318 2944 3810 4564 1318 Size: 1626 866 754 6502 Fragments arranged by size: 6502 1626 866 754 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 5112 5602 5883 Size: 416 53 83 399 24 3695 490 281 Cuts at: 5883 6369 7479 8437 442 Size: 486 1110 958 1753 Fragments arranged by size: 3695 1753 1110 958 490 486 416 399 281 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 9748 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3070 3649 4594 5709 3070 Size: 579 945 1115 7109 Fragments arranged by size: 7109 1115 945 579 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2968 3703 3785 4991 5531 5619 5919 8408 Size: 1729 735 82 1206 540 88 300 2489 Cuts at: 8408 1239 Size: 2579 Fragments arranged by size: 2579 2489 1729 1206 735 540 300 88 82 Bpu10I CC'TnA_GC Cuts at: 2728 2728 Size: 9748 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2426 4121 6457 7873 8738 9036 2426 Size: 1695 2336 1416 865 298 3138 Fragments arranged by size: 3138 2336 1695 1416 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3055 4094 4181 4582 8411 882 Size: 617 1556 1039 87 401 3829 2219 Fragments arranged by size: 3829 2219 1556 1039 617 401 87 BsaAI yAC'GTr Cuts at: 493 1204 4006 7045 7539 493 Size: 711 2802 3039 494 2702 Fragments arranged by size: 3039 2802 2702 711 494 BsaBI GATnn'nnATC Cuts at: 4955 9742 4955 Size: 4787 4961 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3300 3882 4218 7555 Size: 53 83 186 936 1767 582 336 3337 Cuts at: 7555 7937 8572 275 Size: 382 635 1451 Fragments arranged by size: 3337 1767 1451 936 635 582 382 336 186 83 53 BsaWI w'CCGG_w Cuts at: 2508 3167 6627 8181 8579 8853 9000 9411 2508 Size: 659 3460 1554 398 274 147 411 2845 Fragments arranged by size: 3460 2845 1554 659 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2450 2480 3656 3686 3962 Size: 30 165 30 1047 30 1176 30 276 Cuts at: 3962 3992 4325 4349 4355 4379 4671 4701 5333 Size: 30 333 24 6 24 292 30 632 Cuts at: 5333 5363 5643 5673 6476 6506 1178 Size: 30 280 30 803 30 4420 Fragments arranged by size: 4420 1176 1047 803 632 333 292 280 276 165 30 30 30 30 30 30 30 30 30 24 24 6 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3043 3278 3658 3661 4692 4999 5349 1186 Size: 1857 235 380 3 1031 307 350 5585 Fragments arranged by size: 5585 1857 1031 380 350 307 235 3 BseYI C'CCAG_C Cuts at: 2787 3752 4266 4271 4500 4798 4911 5010 5172 Size: 965 514 5 229 298 113 99 162 Cuts at: 5172 5542 5585 6142 8951 9447 2787 Size: 370 43 557 2809 496 3088 Fragments arranged by size: 3088 2809 965 557 514 496 370 298 229 162 113 99 43 5 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2774 2797 2951 3014 4265 5796 6183 Size: 573 1038 23 154 63 1251 1531 387 Cuts at: 6183 1163 Size: 4728 Fragments arranged by size: 4728 1531 1251 1038 573 387 154 63 23 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4584 7198 7959 8987 1540 Size: 275 186 2583 2614 761 1028 2301 Fragments arranged by size: 2614 2583 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2556 2664 2967 3027 3095 3332 4173 4302 5517 Size: 108 303 60 68 237 841 129 1215 Cuts at: 5517 6819 7809 7894 8965 2556 Size: 1302 990 85 1071 3339 Fragments arranged by size: 3339 1302 1215 1071 990 841 303 237 129 108 85 68 60 BsiWI C'GTAC_G Cuts at: 3513 3513 Size: 9748 BsmI GAATG_Cn' Cuts at: 6182 7259 7352 6182 Size: 1077 93 8578 Fragments arranged by size: 8578 1077 93 BsmBI CGTCTCn'nnnn_ Cuts at: 4004 4004 Size: 9748 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2556 2664 2967 2984 3027 3095 3120 Size: 399 766 108 303 17 43 68 25 Cuts at: 3120 3332 3944 4173 4302 4401 4459 4601 4891 Size: 212 612 229 129 99 58 142 290 Cuts at: 4891 5380 5388 5517 5586 6279 6443 6819 7067 Size: 489 8 129 69 693 164 376 248 Cuts at: 7067 7809 7894 8965 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 766 742 693 612 489 399 376 303 290 248 229 212 164 142 129 129 108 99 85 69 68 58 43 25 17 8 BspEI T'CCGG_A Cuts at: 6627 9411 6627 Size: 2784 6964 BspHI T'CATG_A Cuts at: 6577 7637 9367 6577 Size: 1060 1730 6958 Fragments arranged by size: 6958 1730 1060 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2714 4185 5526 7103 844 Size: 1870 1471 1341 1577 3489 Fragments arranged by size: 3489 1870 1577 1471 1341 BsrBI CCG'CTC Cuts at: 1394 1823 3580 4623 5031 5064 7635 1394 Size: 429 1757 1043 408 33 2571 3507 Fragments arranged by size: 3507 2571 1757 1043 429 408 33 BsrDI GCAATG_nn' Cuts at: 65 1248 2719 8243 8417 65 Size: 1183 1471 5524 174 1396 Fragments arranged by size: 5524 1471 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2569 2816 3713 4211 8392 8579 1700 Size: 110 759 247 897 498 4181 187 2869 Fragments arranged by size: 4181 2869 897 759 498 247 187 110 BsrGI T'GTAC_A Cuts at: 96 3249 5409 6889 7002 96 Size: 3153 2160 1480 113 2842 Fragments arranged by size: 3153 2842 2160 1480 113 BssHII G'CGCG_C Cuts at: 1925 2029 1925 Size: 104 9644 BssSI C'ACGA_G Cuts at: 2960 7808 8820 2960 Size: 4848 1012 3888 Fragments arranged by size: 4848 3888 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2595 4177 5090 794 Size: 1801 1582 913 5452 Fragments arranged by size: 5452 1801 1582 913 BstBI TT'CG_AA Cuts at: 2007 8552 2007 Size: 6545 3203 BstEII G'GTnAC_C Cuts at: 1184 3232 4090 4639 1184 Size: 2048 858 549 6293 Fragments arranged by size: 6293 2048 858 549 BstXI CCAn_nnnn'nTGG Cuts at: 3067 4549 4811 4864 3067 Size: 1482 262 53 7951 Fragments arranged by size: 7951 1482 262 53 BstYI r'GATC_y Cuts at: 1972 2760 4746 5963 5995 6387 7078 7445 7830 Size: 788 1986 1217 32 392 691 367 385 Cuts at: 7830 7847 9299 9385 9397 9743 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1986 1977 1452 1217 788 691 392 385 367 346 86 32 17 12 Bsu36I CC'TnA_GG Cuts at: 6289 6289 Size: 9748 BtgI C'CryG_G Cuts at: 513 1065 2689 2792 2897 3213 3360 3675 3957 Size: 552 1624 103 105 316 147 315 282 Cuts at: 3957 4095 4260 4368 4548 4703 5212 6086 6189 Size: 138 165 108 180 155 509 874 103 Cuts at: 6189 6279 6498 513 Size: 90 219 3763 Fragments arranged by size: 3763 1624 874 552 509 316 315 282 219 180 165 155 147 138 108 105 103 103 90 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 9009 Fragments arranged by size: 9009 683 56 BtsI GCAGTG_nn' Cuts at: 5050 5728 5808 7264 8058 8078 5050 Size: 678 80 1456 794 20 6720 Fragments arranged by size: 6720 1456 794 678 80 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 9748 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 9713 DraI TTT'AAA Cuts at: 7052 7052 Size: 9748 DraIII CAC_nnn'GTG Cuts at: 5090 5090 Size: 9748 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 8755 809 Size: 507 7439 1802 Fragments arranged by size: 7439 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2571 3012 3321 Size: 54 1425 50 275 18 741 441 309 Cuts at: 3321 3337 3439 3651 4581 4992 5235 6181 6342 Size: 16 102 212 930 411 243 946 161 Cuts at: 6342 7195 8084 9713 8 Size: 853 889 1629 43 Fragments arranged by size: 1629 1425 946 930 889 853 741 441 411 309 275 243 212 161 102 54 50 43 18 16 EagI C'GGCC_G Cuts at: 1537 1812 4581 7195 1537 Size: 275 2769 2614 4090 Fragments arranged by size: 4090 2769 2614 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2060 2652 2990 3402 5574 5820 7678 1353 Size: 707 592 338 412 2172 246 1858 3423 Fragments arranged by size: 3423 2172 1858 707 592 412 338 246 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4130 6484 8335 8709 8855 387 Size: 1064 138 2541 2354 1851 374 146 1280 Fragments arranged by size: 2541 2354 1851 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2662 3093 3330 4300 2662 Size: 431 237 970 8110 Fragments arranged by size: 8110 970 431 237 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2513 2968 3571 3703 3763 3785 3802 Size: 393 881 455 603 132 60 22 17 Cuts at: 3802 4312 4991 5531 5619 5893 5919 7811 8408 Size: 510 679 540 88 274 26 1892 597 Cuts at: 8408 9195 9463 1239 Size: 787 268 1524 Fragments arranged by size: 1892 1524 881 787 679 603 597 540 510 455 393 274 268 132 88 60 26 22 17 EcoNI CCTnn'n_nnAGG Cuts at: 3608 3608 Size: 9748 EcoO109I rG'GnC_Cy Cuts at: 3310 4129 4456 4687 5007 5106 6147 3310 Size: 819 327 231 320 99 1041 6911 Fragments arranged by size: 6911 1041 819 327 320 231 99 EcoRI G'AATT_C Cuts at: 5986 6512 7057 7435 5986 Size: 526 545 378 8299 Fragments arranged by size: 8299 545 526 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3367 5478 3367 Size: 2111 7637 FspI TGC'GCA Cuts at: 8254 8254 Size: 9748 HaeII r_GCGC'y Cuts at: 1678 2865 3213 3786 4212 4430 8895 1678 Size: 1187 348 573 426 218 4465 2531 Fragments arranged by size: 4465 2531 1187 573 426 348 218 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3365 3397 3794 3826 3962 Size: 32 135 32 1793 32 397 32 136 Cuts at: 3962 3994 4349 4381 4671 4703 4733 4765 4910 Size: 32 355 32 290 32 30 32 145 Cuts at: 4910 4942 5333 5365 6019 6051 6131 6163 8389 Size: 32 391 32 654 32 80 32 2226 Cuts at: 8389 8421 8463 8495 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2226 1793 654 397 391 355 290 145 136 135 80 42 32 32 32 32 32 32 32 32 32 32 32 32 32 32 30 HincII GTy'rAC Cuts at: 669 1657 1938 5326 7092 7338 669 Size: 988 281 3388 1766 246 3079 Fragments arranged by size: 3388 3079 1766 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 9748 HpaI GTT'AAC Cuts at: 7338 7338 Size: 9748 KpnI G_GTAC'C Cuts at: 7073 7073 Size: 9748 MfeI C'AATT_G Cuts at: 7347 7347 Size: 9748 MluI A'CGCG_T Cuts at: 9624 9624 Size: 9748 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2928 3576 3819 4314 4368 4881 4911 8862 Size: 1623 648 243 495 54 513 30 3951 Cuts at: 8862 9046 1305 Size: 184 2007 Fragments arranged by size: 3951 2007 1623 648 513 495 243 184 54 30 MscI TGG'CCA Cuts at: 10 64 1489 1832 3014 3339 3653 6344 10 Size: 54 1425 343 1182 325 314 2691 3414 Fragments arranged by size: 3414 2691 1425 1182 343 325 314 54 MslI CAynn'nnrTG Cuts at: 518 1166 3161 3398 3947 4367 6008 6203 7706 Size: 648 1995 237 549 420 1641 195 1503 Cuts at: 7706 8065 8224 518 Size: 359 159 2042 Fragments arranged by size: 2042 1995 1641 1503 648 549 420 359 237 195 159 MspA1I CmG'CkG Cuts at: 1606 2241 2674 2749 2794 3114 3269 3677 3999 Size: 635 433 75 45 320 155 408 322 Cuts at: 3999 4405 4519 4731 5058 5214 5240 5542 6500 Size: 406 114 212 327 156 26 302 958 Cuts at: 6500 6969 7164 7841 8989 9234 1606 Size: 469 195 677 1148 245 2120 Fragments arranged by size: 2120 1148 958 677 635 469 433 408 406 327 322 320 302 245 212 195 156 155 114 75 45 26 NaeI GCC'GGC Cuts at: 2571 3715 4213 2571 Size: 1144 498 8106 Fragments arranged by size: 8106 1144 498 NcoI C'CATG_G Cuts at: 513 1065 2689 4095 4548 6189 6279 513 Size: 552 1624 1406 453 1641 90 3982 Fragments arranged by size: 3982 1641 1624 1406 552 453 90 NdeI CA'TA_TG Cuts at: 387 387 Size: 9748 NgoMIV G'CCGG_C Cuts at: 2569 3713 4211 2569 Size: 1144 498 8106 Fragments arranged by size: 8106 1144 498 NheI G'CTAG_C Cuts at: 1052 1052 Size: 9748 NotI GC'GGCC_GC Cuts at: 7195 7195 Size: 9748 NspI r_CATG'y Cuts at: 1132 1229 4561 4612 7106 8651 1132 Size: 97 3332 51 2494 1545 2229 Fragments arranged by size: 3332 2494 2229 1545 97 51 PasI CC'CwG_GG Cuts at: 1348 4594 5245 1348 Size: 3246 651 5851 Fragments arranged by size: 5851 3246 651 PciI A'CATG_T Cuts at: 8647 8647 Size: 9748 PflMI CCAn_nnn'nTGG Cuts at: 1108 2333 2455 6190 1108 Size: 1225 122 3735 4666 Fragments arranged by size: 4666 3735 1225 122 PfoI T'CCnGG_A Cuts at: 4959 4959 Size: 9748 PmeI GTTT'AAAC Cuts at: 7052 7052 Size: 9748 PmlI CAC'GTG Cuts at: 4006 4006 Size: 9748 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2711 2743 7817 7849 9330 9362 2711 Size: 32 5074 32 1481 32 3097 Fragments arranged by size: 5074 3097 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 4129 4687 5007 6147 4129 Size: 558 320 1140 7730 Fragments arranged by size: 7730 1140 558 320 PshAI GACnn'nnGTC Cuts at: 1613 4812 1613 Size: 3199 6549 PsiI TTA'TAA Cuts at: 6723 6843 7318 6723 Size: 120 475 9153 Fragments arranged by size: 9153 475 120 PspOMI G'GGCC_C Cuts at: 4455 4455 Size: 9748 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 5759 5791 5759 Size: 32 9716 PstI C_TGCA'G Cuts at: 830 2711 3879 3998 4155 4182 4518 4617 5523 Size: 1881 1168 119 157 27 336 99 906 Cuts at: 5523 5947 7100 830 Size: 424 1153 3478 Fragments arranged by size: 3478 1881 1168 1153 906 424 336 157 119 99 27 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 9748 PvuII CAG'CTG Cuts at: 2674 2749 4405 4519 4731 5542 2674 Size: 75 1656 114 212 811 6880 Fragments arranged by size: 6880 1656 811 212 114 75 SacI G_AGCT'C Cuts at: 2664 3095 3332 4302 2664 Size: 431 237 970 8110 Fragments arranged by size: 8110 970 431 237 SacII CC_GC'GG Cuts at: 2795 3678 5215 6501 2795 Size: 883 1537 1286 6042 Fragments arranged by size: 6042 1537 1286 883 SalI G'TCGA_C Cuts at: 1936 7090 1936 Size: 5154 4594 SanDI GG'GwC_CC Cuts at: 5007 5007 Size: 9748 SapI GCTCTTCn'nnn_ Cuts at: 2060 2652 3402 5574 5820 2060 Size: 592 750 2172 246 5988 Fragments arranged by size: 5988 2172 750 592 246 SbfI CC_TGCA'GG Cuts at: 3879 7100 3879 Size: 3221 6527 ScaI AGT'ACT Cuts at: 5777 6830 7996 9708 5777 Size: 1053 1166 1712 5817 Fragments arranged by size: 5817 1712 1166 1053 SexAI A'CCwGG_T Cuts at: 3063 3063 Size: 9748 SfcI C'TryA_G Cuts at: 826 1045 2130 2707 3875 3994 4151 4178 4492 Size: 219 1085 577 1168 119 157 27 314 Cuts at: 4492 4514 4613 4726 5519 5716 5943 5973 6015 Size: 22 99 113 793 197 227 30 42 Cuts at: 6015 7096 8231 8912 9103 826 Size: 1081 1135 681 191 1471 Fragments arranged by size: 1471 1168 1135 1085 1081 793 681 577 314 227 219 197 191 157 119 113 99 42 30 27 22 SfiI GGCCn_nnn'nGGCC Cuts at: 4589 4589 Size: 9748 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 9748 SgrAI Cr'CCGG_yG Cuts at: 1700 4211 1700 Size: 2511 7237 SmaI CCC'GGG Cuts at: 1872 3574 4233 4886 7075 1872 Size: 1702 659 653 2189 4545 Fragments arranged by size: 4545 2189 1702 659 653 SmlI C'TyrA_G Cuts at: 820 1017 1943 2441 4136 6436 7852 8753 9015 Size: 197 926 498 1695 2300 1416 901 262 Cuts at: 9015 820 Size: 1553 Fragments arranged by size: 2300 1695 1553 1416 926 901 498 262 197 SnaBI TAC'GTA Cuts at: 493 7045 7539 493 Size: 6552 494 2702 Fragments arranged by size: 6552 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 9748 SphI G_CATG'C Cuts at: 1132 4612 7106 1132 Size: 3480 2494 3774 Fragments arranged by size: 3774 3480 2494 SrfI GCCC'GGGC Cuts at: 4886 4886 Size: 9748 SspI AAT'ATT Cuts at: 5 52 7672 5 Size: 47 7620 2081 Fragments arranged by size: 7620 2081 47 StuI AGG'CCT Cuts at: 1517 3528 3974 1517 Size: 2011 446 7291 Fragments arranged by size: 7291 2011 446 StyI C'CwwG_G Cuts at: 513 1065 2689 2886 3609 4095 4548 4603 5109 Size: 552 1624 197 723 486 453 55 506 Cuts at: 5109 5380 5646 6189 6219 6279 7033 7187 513 Size: 271 266 543 30 60 754 154 3074 Fragments arranged by size: 3074 1624 754 723 552 543 506 486 453 271 266 197 154 60 55 30 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3101 3638 3929 4481 4658 6267 7775 Size: 1255 1521 537 291 552 177 1609 1508 Cuts at: 7775 7792 7945 8130 8575 325 Size: 17 153 185 445 1498 Fragments arranged by size: 1609 1521 1508 1498 1255 552 537 445 291 185 177 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3249 4875 5354 Size: 274 80 33 51 1443 1272 1626 479 Cuts at: 5354 5409 5775 6019 6326 6828 6889 7002 7994 Size: 55 366 244 307 502 61 113 992 Cuts at: 7994 9706 96 Size: 1712 138 Fragments arranged by size: 1712 1626 1443 1272 992 502 479 366 307 274 244 138 113 80 61 55 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 9708 9740 9708 Size: 32 9716 Tth111I GACn'n_nGTC Cuts at: 1316 2543 5005 1316 Size: 1227 2462 6059 Fragments arranged by size: 6059 2462 1227 XbaI T'CTAG_A Cuts at: 7084 7084 Size: 9748 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3064 3760 3064 Size: 696 9052 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 9748 XmaI C'CCGG_G Cuts at: 1870 3572 4231 4884 7073 1870 Size: 1702 659 653 2189 4545 Fragments arranged by size: 4545 2189 1702 659 653 XmnI GAAnn'nnTTC Cuts at: 7877 7877 Size: 9748 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 7556 276 Size: 53 83 186 936 6022 2468 Fragments arranged by size: 6022 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI PvuII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI BaeI BbeI BbvCI BfrBI BstZ17I EcoRV FseI FspAI KasI NarI NruI NsiI PacI PspXI RsrII SfoI SwaI