(Circular) (Six-base) MAPSORT of: pFN21ASDA0821.seq Check: 3043 from: 1 to: 9151 pFN21ASDA0821 9151 bp With 182 enzymes: SgfI * January 16, 2009 15:30 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3943 5583 3943 Size: 1640 7511 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2187 3732 3984 6961 Size: 53 83 186 936 651 1545 252 2977 Cuts at: 6961 278 Size: 2468 Fragments arranged by size: 2977 2468 1545 936 651 252 186 83 53 AccI GT'mk_AC Cuts at: 1937 2676 3568 4472 6494 1937 Size: 739 892 904 2022 4594 Fragments arranged by size: 4594 2022 904 892 739 Acc65I G'GTAC_C Cuts at: 5558 6472 5558 Size: 914 8237 AclI AA'CG_TT Cuts at: 1563 7278 7651 1563 Size: 5715 373 3063 Fragments arranged by size: 5715 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 7214 8598 8866 1632 Size: 5582 1384 268 1917 Fragments arranged by size: 5582 1917 1384 268 AfeI AGC'GCT Cuts at: 2960 2960 Size: 9151 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 8954 AflIII A'CryG_T Cuts at: 1203 3439 3441 4517 8050 9027 1203 Size: 2236 2 1076 3533 977 1327 Fragments arranged by size: 3533 2236 1327 1076 977 2 AgeI A'CCGG_T Cuts at: 7982 7982 Size: 9151 AhdI GACnn_n'nnGTC Cuts at: 7880 7880 Size: 9151 AleI CACnn'nnGTG Cuts at: 1166 2305 4616 1166 Size: 1139 2311 5701 Fragments arranged by size: 5701 2311 1139 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 5372 1184 Size: 4188 4963 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4094 4126 4094 Size: 32 9119 AlwNI CAG_nnn'CTG Cuts at: 3615 3763 3793 4105 6894 8466 3615 Size: 148 30 312 2789 1572 4300 Fragments arranged by size: 4300 2789 1572 312 148 30 ApaI G_GGCC'C Cuts at: 3602 5819 5930 5997 6070 6237 6408 3602 Size: 2217 111 67 73 167 171 6345 Fragments arranged by size: 6345 2217 171 167 111 73 67 ApaLI G'TGCA_C Cuts at: 3956 4859 5333 5888 7208 8364 3956 Size: 903 474 555 1320 1156 4743 Fragments arranged by size: 4743 1320 1156 903 555 474 ApoI r'AATT_y Cuts at: 1493 5466 6460 6686 6838 8947 1493 Size: 3973 994 226 152 2109 1697 Fragments arranged by size: 3973 2109 1697 994 226 152 AseI AT'TA_AT Cuts at: 160 1032 7705 160 Size: 872 6673 1606 Fragments arranged by size: 6673 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 9151 AvaI C'yCGr_G Cuts at: 1870 1943 3468 3543 3594 3705 4129 4279 5390 Size: 73 1525 75 51 111 424 150 1111 Cuts at: 5390 5804 5829 5996 6063 6476 1870 Size: 414 25 167 67 413 4545 Fragments arranged by size: 4545 1525 1111 424 414 413 167 150 111 75 73 67 51 25 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 5542 5575 5542 Size: 33 9118 BamHI G'GATC_C Cuts at: 2559 5405 6481 6848 2559 Size: 2846 1076 367 4862 Fragments arranged by size: 4862 2846 1076 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2169 2684 3331 3356 3995 Size: 325 217 627 382 515 647 25 639 Cuts at: 3995 4295 4991 5558 5609 6128 6472 7927 618 Size: 300 696 567 51 519 344 1455 1842 Fragments arranged by size: 1842 1455 696 647 639 627 567 519 515 382 344 325 300 217 51 25 BanII G_rGCy'C Cuts at: 1391 2112 2472 3456 3468 3585 3602 3713 5819 Size: 721 360 984 12 117 17 111 2106 Cuts at: 5819 5930 5946 5997 6070 6237 6408 6470 1391 Size: 111 16 51 73 167 171 62 4072 Fragments arranged by size: 4072 2106 984 721 360 171 167 117 111 111 73 62 51 17 16 12 BbeI G_GCGC'C Cuts at: 3999 4299 6132 3999 Size: 300 1833 7018 Fragments arranged by size: 7018 1833 300 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2425 2683 4195 4351 5287 5665 928 Size: 971 526 258 1512 156 936 378 4414 Fragments arranged by size: 4414 1512 971 936 526 378 258 156 BbvCI CC'TCA_GC Cuts at: 3254 3608 5419 3254 Size: 354 1811 6986 Fragments arranged by size: 6986 1811 354 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3209 3243 7342 7376 1903 Size: 34 1272 34 4099 34 3678 Fragments arranged by size: 4099 3678 1272 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 7043 8253 7043 Size: 1210 7941 BclI T'GATC_A Cuts at: 1548 5871 1548 Size: 4323 4828 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4284 4293 4854 Size: 107 122 71 631 605 2612 9 561 Cuts at: 4854 5852 6185 6317 6369 7762 136 Size: 998 333 132 52 1393 1525 Fragments arranged by size: 2612 1525 1393 998 631 605 561 333 132 122 107 71 52 9 BglII A'GATC_T Cuts at: 4569 9146 4569 Size: 4577 4574 BlpI GC'TnA_GC Cuts at: 4740 6568 4740 Size: 1828 7323 Bme1580I G_kGCm'C Cuts at: 1790 2174 2444 2936 3334 3602 3960 4863 5337 Size: 384 270 492 398 268 358 903 474 Cuts at: 5337 5399 5612 5819 5892 5930 5997 6070 6237 Size: 62 213 207 73 38 67 73 167 Cuts at: 6237 6408 7212 8368 1790 Size: 171 804 1156 2573 Fragments arranged by size: 2573 1156 903 804 492 474 398 384 358 270 268 213 207 171 167 73 73 67 62 38 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 9151 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2317 3219 3344 Size: 416 53 83 399 24 900 902 125 Cuts at: 3344 3395 6064 6231 6882 7840 442 Size: 51 2669 167 651 958 1753 Fragments arranged by size: 2669 1753 958 902 900 651 416 399 167 125 83 53 51 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 9151 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2491 2611 2491 Size: 120 9031 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2408 2486 3224 3372 3941 4007 4085 4418 Size: 1169 78 738 148 569 66 78 333 Cuts at: 4418 4871 5056 5125 6062 7811 1239 Size: 453 185 69 937 1749 2579 Fragments arranged by size: 2579 1749 1169 937 738 569 453 333 185 148 78 78 69 66 Bpu10I CC'TnA_GC Cuts at: 2096 3254 3450 3608 5419 5820 5940 2096 Size: 1158 196 158 1811 401 120 5307 Fragments arranged by size: 5307 1811 1158 401 196 158 120 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 5114 5396 6413 7276 8141 8439 5114 Size: 282 1017 863 865 298 5826 Fragments arranged by size: 5826 1017 865 863 298 282 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 7814 882 Size: 617 6315 2219 Fragments arranged by size: 6315 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2548 2611 2937 3019 3442 4518 6448 Size: 711 1344 63 326 82 423 1076 1930 Cuts at: 6448 6942 493 Size: 494 2702 Fragments arranged by size: 2702 1930 1344 1076 711 494 423 326 82 63 BsaBI GATnn'nnATC Cuts at: 5873 9145 5873 Size: 3272 5879 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2184 2283 3729 3981 Size: 53 83 186 936 651 99 1446 252 Cuts at: 3981 3996 4296 6129 6355 6958 7340 7975 275 Size: 15 300 1833 226 603 382 635 1451 Fragments arranged by size: 1833 1451 1446 936 651 635 603 382 300 252 226 186 99 83 53 15 BsaWI w'CCGG_w Cuts at: 7584 7982 8256 8403 8814 7584 Size: 398 274 147 411 7921 Fragments arranged by size: 7921 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2126 2156 2381 2411 3605 Size: 30 165 30 723 30 225 30 1194 Cuts at: 3605 3635 3719 3749 4094 4124 5762 5792 6191 Size: 30 84 30 345 30 1638 30 399 Cuts at: 6191 6221 1178 Size: 30 4108 Fragments arranged by size: 4108 1638 1194 723 399 345 225 165 84 30 30 30 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3101 3121 3724 3727 3965 4004 4136 5216 Size: 1915 20 603 3 238 39 132 1080 Cuts at: 5216 5650 6059 6139 1186 Size: 434 409 80 4198 Fragments arranged by size: 4198 1915 1080 603 434 409 238 132 80 39 20 3 BseYI C'CCAG_C Cuts at: 2805 3235 3370 3407 3424 3501 3780 4459 5173 Size: 430 135 37 17 77 279 679 714 Cuts at: 5173 5200 8354 8850 2805 Size: 27 3154 496 3106 Fragments arranged by size: 3154 3106 714 679 496 430 279 135 77 37 27 17 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2285 2414 3601 4118 4796 5084 6097 Size: 573 549 129 1187 517 678 288 1013 Cuts at: 6097 1163 Size: 4217 Fragments arranged by size: 4217 1187 1013 678 573 549 517 288 129 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4696 6120 6601 7362 8390 1540 Size: 275 186 2695 1424 481 761 1028 2301 Fragments arranged by size: 2695 2301 1424 1028 761 481 275 186 BsiHKAI G_wGCw'C Cuts at: 2046 2496 3456 3960 4554 4863 5130 5337 5892 Size: 450 960 504 594 309 267 207 555 Cuts at: 5892 7212 7297 8368 2046 Size: 1320 85 1071 2829 Fragments arranged by size: 2829 1320 1071 960 594 555 504 450 309 267 207 85 BsmI GAATG_Cn' Cuts at: 2206 3946 4252 6662 6755 2206 Size: 1740 306 2410 93 4602 Fragments arranged by size: 4602 2410 1740 306 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2753 2936 3736 2753 Size: 183 800 8168 Fragments arranged by size: 8168 800 183 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2046 2112 2174 2444 2472 2496 2936 Size: 399 256 66 62 270 28 24 440 Cuts at: 2936 3334 3456 3468 3585 3602 3713 3960 4554 Size: 398 122 12 117 17 111 247 594 Cuts at: 4554 4863 5130 5337 5399 5612 5819 5892 5930 Size: 309 267 207 62 213 207 73 38 Cuts at: 5930 5946 5997 6070 6237 6408 6470 7212 7297 Size: 16 51 73 167 171 62 742 85 Cuts at: 7297 8368 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 594 440 399 398 309 270 267 256 247 213 207 207 171 167 122 117 111 85 73 73 66 62 62 62 51 38 28 24 17 16 12 BspEI T'CCGG_A Cuts at: 8814 8814 Size: 9151 BspHI T'CATG_A Cuts at: 7040 8770 7040 Size: 1730 7421 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2283 3943 4878 5583 5907 6201 6506 844 Size: 1439 1660 935 705 324 294 305 3489 Fragments arranged by size: 3489 1660 1439 935 705 324 305 294 BsrBI CCG'CTC Cuts at: 1394 1823 2754 3322 5382 7038 1394 Size: 429 931 568 2060 1656 3507 Fragments arranged by size: 3507 2060 1656 931 568 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4189 7646 7820 65 Size: 1183 2941 3457 174 1396 Fragments arranged by size: 3457 2941 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2339 3493 3818 4265 4802 6138 7795 Size: 110 529 1154 325 447 537 1336 1657 Cuts at: 7795 7982 1700 Size: 187 2869 Fragments arranged by size: 2869 1657 1336 1154 537 529 447 325 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 9151 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 9151 BssSI C'ACGA_G Cuts at: 2483 2596 6365 7211 8223 2483 Size: 113 3769 846 1012 3411 Fragments arranged by size: 3769 3411 1012 846 113 BstAPI GCAn_nnn'nTGC Cuts at: 794 2325 2461 3511 3793 794 Size: 1531 136 1050 282 6152 Fragments arranged by size: 6152 1531 1050 282 136 BstBI TT'CG_AA Cuts at: 2007 7955 2007 Size: 5948 3203 BstEII G'GTnAC_C Cuts at: 1184 2074 2534 5092 6433 1184 Size: 890 460 2558 1341 3902 Fragments arranged by size: 3902 2558 1341 890 460 BstXI CCAn_nnnn'nTGG Cuts at: 2653 3691 5149 2653 Size: 1038 1458 6655 Fragments arranged by size: 6655 1458 1038 BstYI r'GATC_y Cuts at: 1972 2559 4569 5405 6481 6848 7233 7250 8702 Size: 587 2010 836 1076 367 385 17 1452 Cuts at: 8702 8788 8800 9146 1972 Size: 86 12 346 1977 Fragments arranged by size: 2010 1977 1452 1076 836 587 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 6228 6228 Size: 9151 BtgI C'CryG_G Cuts at: 513 1065 2022 3879 3945 4239 4386 5562 5602 Size: 552 957 1857 66 294 147 1176 40 Cuts at: 5602 513 Size: 4062 Fragments arranged by size: 4062 1857 1176 957 552 294 147 66 40 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2250 3664 4309 4945 5167 5617 Size: 683 56 1024 1414 645 636 222 450 Cuts at: 5617 487 Size: 4021 Fragments arranged by size: 4021 1414 1024 683 645 636 450 222 56 BtsI GCAGTG_nn' Cuts at: 2046 2273 2918 5311 6667 7461 7481 2046 Size: 227 645 2393 1356 794 20 3716 Fragments arranged by size: 3716 2393 1356 794 645 227 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 9151 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 9116 DraI TTT'AAA Cuts at: 6455 6455 Size: 9151 DraIII CAC_nnn'GTG Cuts at: 2325 3352 2325 Size: 1027 8124 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2485 8158 809 Size: 507 1169 5673 1802 Fragments arranged by size: 5673 1802 1169 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2082 2613 3491 Size: 54 1425 50 275 18 252 531 878 Cuts at: 3491 3495 3672 3908 3963 4008 4263 4647 5919 Size: 4 177 236 55 45 255 384 1272 Cuts at: 5919 6117 6160 6598 7487 9116 8 Size: 198 43 438 889 1629 43 Fragments arranged by size: 1629 1425 1272 889 878 531 438 384 275 255 252 236 198 177 55 54 50 45 43 43 18 4 EagI C'GGCC_G Cuts at: 1537 1812 6117 6598 1537 Size: 275 4305 481 4090 Fragments arranged by size: 4305 4090 481 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3461 4235 4736 4754 5234 5270 5976 7081 Size: 2108 774 501 18 480 36 706 1105 Cuts at: 7081 1353 Size: 3423 Fragments arranged by size: 3423 2108 1105 774 706 501 480 36 18 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2826 3314 7738 8112 8258 387 Size: 1064 138 1237 488 4424 374 146 1280 Fragments arranged by size: 4424 1280 1237 1064 488 374 146 138 EcoICRI GAG'CTC Cuts at: 3454 3454 Size: 9151 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2408 2486 3224 3372 3941 4007 4085 Size: 393 776 78 738 148 569 66 78 Cuts at: 4085 4418 4871 5056 5125 6062 7214 7811 8598 Size: 333 453 185 69 937 1152 597 787 Cuts at: 8598 8866 1239 Size: 268 1524 Fragments arranged by size: 1524 1152 937 787 776 738 597 569 453 393 333 268 185 148 78 78 69 66 EcoNI CCTnn'n_nnAGG Cuts at: 2456 3989 5961 2456 Size: 1533 1972 5646 Fragments arranged by size: 5646 1972 1533 EcoO109I rG'GnC_Cy Cuts at: 2373 2448 2518 2992 3374 3525 3598 3599 3790 Size: 75 70 474 382 151 73 1 191 Cuts at: 3790 5454 5815 5816 5926 5927 6037 6233 6244 Size: 1664 361 1 110 1 110 196 11 Cuts at: 6244 6404 2373 Size: 160 5120 Fragments arranged by size: 5120 1664 474 382 361 196 191 160 151 110 110 75 73 70 11 1 1 1 EcoRI G'AATT_C Cuts at: 5466 6460 6838 5466 Size: 994 378 7779 Fragments arranged by size: 7779 994 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 4429 5728 4429 Size: 1299 7852 FseI GG_CCGG'CC Cuts at: 3497 3497 Size: 9151 FspI TGC'GCA Cuts at: 7657 7657 Size: 9151 HaeII r_GCGC'y Cuts at: 1678 2962 3999 4299 5174 6132 8298 1678 Size: 1284 1037 300 875 958 2166 2531 Fragments arranged by size: 2531 2166 1284 1037 958 875 300 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2483 2515 2945 2977 3719 Size: 32 135 32 911 32 430 32 742 Cuts at: 3719 3751 3797 3829 6191 6223 7792 7824 7866 Size: 32 46 32 2362 32 1569 32 42 Cuts at: 7866 7898 1373 Size: 32 2626 Fragments arranged by size: 2626 2362 1569 911 742 430 135 46 42 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3139 3634 4473 5074 6495 6741 Size: 988 281 1201 495 839 601 1421 246 Cuts at: 6741 669 Size: 3079 Fragments arranged by size: 3079 1421 1201 988 839 601 495 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 9151 HpaI GTT'AAC Cuts at: 6741 6741 Size: 9151 KasI G'GCGC_C Cuts at: 3995 4295 6128 3995 Size: 300 1833 7018 Fragments arranged by size: 7018 1833 300 KpnI G_GTAC'C Cuts at: 5562 6476 5562 Size: 914 8237 MfeI C'AATT_G Cuts at: 6750 6750 Size: 9151 MluI A'CGCG_T Cuts at: 9027 9027 Size: 9151 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2994 4524 6084 8265 8449 1305 Size: 1689 1530 1560 2181 184 2007 Fragments arranged by size: 2181 2007 1689 1560 1530 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 4649 10 Size: 54 1425 343 2817 4512 Fragments arranged by size: 4512 2817 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2192 2305 2546 3515 4416 4616 7109 Size: 648 1026 113 241 969 901 200 2493 Cuts at: 7109 7468 7627 518 Size: 359 159 2042 Fragments arranged by size: 2493 2042 1026 969 901 648 359 241 200 159 113 MspA1I CmG'CkG Cuts at: 1606 2524 2805 2905 3163 3370 3760 3784 4150 Size: 918 281 100 258 207 390 24 366 Cuts at: 4150 6183 6430 6567 7244 8392 8637 1606 Size: 2033 247 137 677 1148 245 2120 Fragments arranged by size: 2120 2033 1148 918 677 390 366 281 258 247 245 207 137 100 24 NaeI GCC'GGC Cuts at: 2341 3495 3820 4267 4804 6140 2341 Size: 1154 325 447 537 1336 5352 Fragments arranged by size: 5352 1336 1154 537 447 325 NarI GG'CG_CC Cuts at: 3996 4296 6129 3996 Size: 300 1833 7018 Fragments arranged by size: 7018 1833 300 NcoI C'CATG_G Cuts at: 513 1065 2022 5562 513 Size: 552 957 3540 4102 Fragments arranged by size: 4102 3540 957 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 9151 NgoMIV G'CCGG_C Cuts at: 2339 3493 3818 4265 4802 6138 2339 Size: 1154 325 447 537 1336 5352 Fragments arranged by size: 5352 1336 1154 537 447 325 NheI G'CTAG_C Cuts at: 1052 1052 Size: 9151 NotI GC'GGCC_GC Cuts at: 6598 6598 Size: 9151 NruI TCG'CGA Cuts at: 3153 3153 Size: 9151 NspI r_CATG'y Cuts at: 1132 1229 3514 6509 8054 1132 Size: 97 2285 2995 1545 2229 Fragments arranged by size: 2995 2285 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 2444 3627 4289 5684 1348 Size: 1096 1183 662 1395 4815 Fragments arranged by size: 4815 1395 1183 1096 662 PciI A'CATG_T Cuts at: 8050 8050 Size: 9151 PflMI CCAn_nnn'nTGG Cuts at: 1108 2329 1108 Size: 1221 7930 PfoI T'CCnGG_A Cuts at: 3771 3926 3984 4062 3771 Size: 155 58 78 8860 Fragments arranged by size: 8860 155 78 58 PmeI GTTT'AAAC Cuts at: 6455 6455 Size: 9151 PmlI CAC'GTG Cuts at: 2937 3442 4518 2937 Size: 505 1076 7570 Fragments arranged by size: 7570 1076 505 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4094 4126 7220 7252 8733 8765 4094 Size: 32 3094 32 1481 32 4480 Fragments arranged by size: 4480 3094 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2373 2448 2518 2992 3374 5454 2373 Size: 75 70 474 382 2080 6070 Fragments arranged by size: 6070 2080 474 382 75 70 PshAI GACnn'nnGTC Cuts at: 1613 2364 1613 Size: 751 8400 PsiI TTA'TAA Cuts at: 6721 6721 Size: 9151 PspOMI G'GGCC_C Cuts at: 3598 5815 5926 5993 6066 6233 6404 3598 Size: 2217 111 67 73 167 171 6345 Fragments arranged by size: 6345 2217 171 167 111 73 67 PspXI vC'TCGA_Gb Cuts at: 3543 3543 Size: 9151 PstI C_TGCA'G Cuts at: 830 2457 2529 3789 4529 4689 5598 6336 6503 Size: 1627 72 1260 740 160 909 738 167 Cuts at: 6503 830 Size: 3478 Fragments arranged by size: 3478 1627 1260 909 740 738 167 160 72 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 9151 PvuII CAG'CTG Cuts at: 2905 3163 3370 3760 3784 4150 6430 2905 Size: 258 207 390 24 366 2280 5626 Fragments arranged by size: 5626 2280 390 366 258 207 24 SacI G_AGCT'C Cuts at: 3456 3456 Size: 9151 SalI G'TCGA_C Cuts at: 1936 4471 6493 1936 Size: 2535 2022 4594 Fragments arranged by size: 4594 2535 2022 SanDI GG'GwC_CC Cuts at: 2448 5454 2448 Size: 3006 6145 SapI GCTCTTCn'nnn_ Cuts at: 3461 4754 3461 Size: 1293 7858 SbfI CC_TGCA'GG Cuts at: 6336 6503 6336 Size: 167 8984 ScaI AGT'ACT Cuts at: 4916 7399 9111 4916 Size: 2483 1712 4956 Fragments arranged by size: 4956 2483 1712 SexAI A'CCwGG_T Cuts at: 3636 5076 6345 3636 Size: 1440 1269 6442 Fragments arranged by size: 6442 1440 1269 SfcI C'TryA_G Cuts at: 826 1045 2453 2525 3196 3785 4525 4685 5594 Size: 219 1408 72 671 589 740 160 909 Cuts at: 5594 6332 6499 7634 8315 8506 826 Size: 738 167 1135 681 191 1471 Fragments arranged by size: 1471 1408 1135 909 740 738 681 671 589 219 191 167 160 72 SfiI GGCCn_nnn'nGGCC Cuts at: 4284 4284 Size: 9151 SfoI GGC'GCC Cuts at: 3997 4297 6130 3997 Size: 300 1833 7018 Fragments arranged by size: 7018 1833 300 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 9151 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 9151 SmaI CCC'GGG Cuts at: 1872 3596 3707 4281 5392 5806 5998 6065 6478 Size: 1724 111 574 1111 414 192 67 413 Cuts at: 6478 1872 Size: 4545 Fragments arranged by size: 4545 1724 1111 574 414 413 192 111 67 SmlI C'TyrA_G Cuts at: 820 1017 1943 3543 5129 5411 6392 7255 8156 Size: 197 926 1600 1586 282 981 863 901 Cuts at: 8156 8418 820 Size: 262 1553 Fragments arranged by size: 1600 1586 1553 981 926 901 863 282 262 197 SnaBI TAC'GTA Cuts at: 493 6448 6942 493 Size: 5955 494 2702 Fragments arranged by size: 5955 2702 494 SpeI A'CTAG_T Cuts at: 152 4876 152 Size: 4724 4427 SphI G_CATG'C Cuts at: 1132 3514 6509 1132 Size: 2382 2995 3774 Fragments arranged by size: 3774 2995 2382 SspI AAT'ATT Cuts at: 5 52 7075 5 Size: 47 7023 2081 Fragments arranged by size: 7023 2081 47 StuI AGG'CCT Cuts at: 1517 2094 5003 6390 1517 Size: 577 2909 1387 4278 Fragments arranged by size: 4278 2909 1387 577 StyI C'CwwG_G Cuts at: 513 1065 2022 2089 3534 3576 4047 4477 5562 Size: 552 957 67 1445 42 471 430 1085 Cuts at: 5562 5712 5922 6590 513 Size: 150 210 668 3074 Fragments arranged by size: 3074 1445 1085 957 668 552 471 430 210 150 67 42 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2102 2336 2834 3344 3363 3515 4594 Size: 1255 522 234 498 510 19 152 1079 Cuts at: 4594 4606 4706 4916 7178 7195 7348 7533 7978 Size: 12 100 210 2262 17 153 185 445 Cuts at: 7978 325 Size: 1498 Fragments arranged by size: 2262 1498 1255 1079 522 510 498 445 234 210 185 153 152 100 19 17 12 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4914 5343 7397 Size: 274 80 33 51 1443 2937 429 2054 Cuts at: 7397 9109 96 Size: 1712 138 Fragments arranged by size: 2937 2054 1712 1443 429 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2041 2073 2126 2158 9111 9143 2041 Size: 32 53 32 6953 32 2049 Fragments arranged by size: 6953 2049 53 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2405 4016 4373 5506 1316 Size: 1089 1611 357 1133 4961 Fragments arranged by size: 4961 1611 1133 1089 357 XbaI T'CTAG_A Cuts at: 6487 6487 Size: 9151 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2977 3349 3367 4054 4258 5710 2977 Size: 372 18 687 204 1452 6418 Fragments arranged by size: 6418 1452 687 372 204 18 XhoI C'TCGA_G Cuts at: 1943 3543 1943 Size: 1600 7551 XmaI C'CCGG_G Cuts at: 1870 3594 3705 4279 5390 5804 5996 6063 6476 Size: 1724 111 574 1111 414 192 67 413 Cuts at: 6476 1870 Size: 4545 Fragments arranged by size: 4545 1724 1111 574 414 413 192 111 67 XmnI GAAnn'nnTTC Cuts at: 2432 4412 7280 2432 Size: 1980 2868 4303 Fragments arranged by size: 4303 2868 1980 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2185 3730 3982 6959 Size: 53 83 186 936 651 1545 252 2977 Cuts at: 6959 276 Size: 2468 Fragments arranged by size: 2977 2468 1545 936 651 252 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FseI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI SalI SanDI SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AscI AvrII BfrBI BsiWI BstZ17I EcoRV FspAI NsiI PacI PsrI RsrII SacII SrfI SwaI