Restriction map for
pFN21ASDA0813
(Linear) (Six-base) MAPSORT of: KIAA0813.seq Check: 227 from: 1 to: 4602
KIAA0813.orf
With 182 enzymes: SgfI *
October 24, 2006 15:14 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 124 829 868 2261 2824 2941 3091 3217
Size: 124 705 39 1393 563 117 150 126
Cuts at: 3217 4602
Size: 1385
Fragments arranged by size:
1393 1385 705 563 150 126 124 117
39
AccI GT'mk_AC
Cuts at: 0 2341 4602
Size: 2341 2261
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1606 1720 2133 4528 4602
Size: 1606 114 413 2395 74
Fragments arranged by size:
2395 1606 413 114 74
AflII C'TTAA_G
Cuts at: 0 2394 4602
Size: 2394 2208
AflIII A'CryG_T
Cuts at: 0 365 4602
Size: 365 4237
AleI CACnn'nnGTG
Cuts at: 0 2208 4154 4602
Size: 2208 1946 448
Fragments arranged by size:
2208 1946 448
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 169 1805 4278 4288 4602
Size: 169 1636 2473 10 314
Fragments arranged by size:
2473 1636 314 169 10
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 367 368 399 400 2947 2979 4602
Size: 367 1 31 1 2547 32 1623
Fragments arranged by size:
2547 1623 367 32 31 1 1
AlwNI CAG_nnn'CTG
Cuts at: 0 687 1155 1209 1299 1768 4281 4602
Size: 687 468 54 90 469 2513 321
Fragments arranged by size:
2513 687 469 468 321 90 54
ApaI G_GGCC'C
Cuts at: 0 733 2262 2687 3200 3288 4079 4251 4602
Size: 733 1529 425 513 88 791 172 351
Fragments arranged by size:
1529 791 733 513 425 351 172 88
ApaLI G'TGCA_C
Cuts at: 0 855 4602
Size: 855 3747
ApoI r'AATT_y
Cuts at: 0 480 4602
Size: 480 4122
AvaI C'yCGr_G
Cuts at: 0 13 726 922 4221 4602
Size: 13 713 196 3299 381
Fragments arranged by size:
3299 713 381 196 13
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1937 1970 4602
Size: 1937 33 2632
Fragments arranged by size:
2632 1937 33
BamHI G'GATC_C
Cuts at: 0 224 3602 4602
Size: 224 3378 1000
Fragments arranged by size:
3378 1000 224
BanI G'GyrC_C
Cuts at: 0 80 88 1387 1409 2590 2812 2929 3184
Size: 80 8 1299 22 1181 222 117 255
Cuts at: 3184 3846 3919 4005 4306 4602
Size: 662 73 86 301 296
Fragments arranged by size:
1299 1181 662 301 296 255 222 117
86 80 73 22 8
BanII G_rGCy'C
Cuts at: 0 53 251 713 733 2262 2687 3200 3288
Size: 53 198 462 20 1529 425 513 88
Cuts at: 3288 3388 3799 4079 4251 4262 4471 4602
Size: 100 411 280 172 11 209 131
Fragments arranged by size:
1529 513 462 425 411 280 209 198
172 131 100 88 53 20 11
BbeI G_GCGC'C
Cuts at: 0 84 1413 3850 4602
Size: 84 1329 2437 752
Fragments arranged by size:
2437 1329 752 84
BbsI GAAGACnn'nnnn_
Cuts at: 0 2650 4602
Size: 2650 1952
BbvCI CC'TCA_GC
Cuts at: 0 688 2443 3329 4602
Size: 688 1755 886 1273
Fragments arranged by size:
1755 1273 886 688
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 855 889 3616 3650 4602
Size: 855 34 2727 34 952
Fragments arranged by size:
2727 952 855 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 1965 4602
Size: 1965 2637
BglI GCCn_nnn'nGGC
Cuts at: 0 42 436 746 910 2861 3464 4046 4286
Size: 42 394 310 164 1951 603 582 240
Cuts at: 4286 4602
Size: 316
Fragments arranged by size:
1951 603 582 394 316 310 240 164
42
BlpI GC'TnA_GC
Cuts at: 0 1461 4602
Size: 1461 3141
Bme1580I G_kGCm'C
Cuts at: 0 103 733 818 859 1270 1392 2203 2262
Size: 103 630 85 41 411 122 811 59
Cuts at: 2262 2687 2815 2932 3094 3187 3200 3288 4079
Size: 425 128 117 162 93 13 88 791
Cuts at: 4079 4178 4230 4251 4320 4477 4593 4602
Size: 99 52 21 69 157 116 9
Fragments arranged by size:
811 791 630 425 411 162 157 128
122 117 116 103 99 93 88 85
69 59 52 41 21 13 9
BmgBI CAC'GTC
Cuts at: 0 1530 2133 2562 4602
Size: 1530 603 429 2040
Fragments arranged by size:
2040 1530 603 429
BmrI ACTGGGnnnn_n'
Cuts at: 0 714 1611 1682 3168 3282 4602
Size: 714 897 71 1486 114 1320
Fragments arranged by size:
1486 1320 897 714 114 71
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 352 568 967 998 1563 1684 1822 1963
Size: 352 216 399 31 565 121 138 141
Cuts at: 1963 4602
Size: 2639
Fragments arranged by size:
2639 565 399 352 216 141 138 121
31
Bpu10I CC'TnA_GC
Cuts at: 0 688 789 1018 1604 2371 2443 3329 4602
Size: 688 101 229 586 767 72 886 1273
Fragments arranged by size:
1273 886 767 688 586 229 101 72
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 235 438 1324 1863 2854 4602
Size: 235 203 886 539 991 1748
Fragments arranged by size:
1748 991 886 539 235 203
BsaI GGTCTCn'nnnn_
Cuts at: 0 919 3585 4602
Size: 919 2666 1017
Fragments arranged by size:
2666 1017 919
BsaAI yAC'GTr
Cuts at: 0 2969 4602
Size: 2969 1633
BsaBI GATnn'nnATC
Cuts at: 0 1657 4602
Size: 1657 2945
BsaHI Gr'CG_yC
Cuts at: 0 81 1052 1410 3053 3847 4602
Size: 81 971 358 1643 794 755
Fragments arranged by size:
1643 971 794 755 358 81
BsaWI w'CCGG_w
Cuts at: 0 121 372 1454 1811 3144 4602
Size: 121 251 1082 357 1333 1458
Fragments arranged by size:
1458 1333 1082 357 251 121
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1985 2015 2551 2581 3298 3328 4602
Size: 1985 30 536 30 717 30 1274
Fragments arranged by size:
1985 1274 717 536 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1912 2185 2701 4573 4602
Size: 1912 273 516 1872 29
Fragments arranged by size:
1912 1872 516 273 29
BseYI C'CCAG_C
Cuts at: 0 732 2636 2680 2857 3691 4100 4602
Size: 732 1904 44 177 834 409 502
Fragments arranged by size:
1904 834 732 502 409 177 44
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 96 102 346 619 807 1285 1765 4096
Size: 96 6 244 273 188 478 480 2331
Cuts at: 4096 4554 4602
Size: 458 48
Fragments arranged by size:
2331 480 478 458 273 244 188 96
48 6
BsiEI CG_ry'CG
Cuts at: 0 46 4602
Size: 46 4556
BsiHKAI G_wGCw'C
Cuts at: 0 53 537 727 859 1462 1579 2974 3142
Size: 53 484 190 132 603 117 1395 168
Cuts at: 3142 4602
Size: 1460
Fragments arranged by size:
1460 1395 603 484 190 168 132 117
53
BsmI GAATG_Cn'
Cuts at: 0 1170 2273 2743 4602
Size: 1170 1103 470 1859
Fragments arranged by size:
1859 1170 1103 470
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1372 3709 4602
Size: 1372 2337 893
Fragments arranged by size:
2337 1372 893
Bsp1286I G_dGCh'C
Cuts at: 0 53 103 251 537 713 727 733 818
Size: 53 50 148 286 176 14 6 85
Cuts at: 818 859 1270 1392 1462 1579 2203 2262 2687
Size: 41 411 122 70 117 624 59 425
Cuts at: 2687 2815 2932 2974 3094 3142 3187 3200 3288
Size: 128 117 42 120 48 45 13 88
Cuts at: 3288 3388 3799 4079 4178 4230 4251 4262 4320
Size: 100 411 280 99 52 21 11 58
Cuts at: 4320 4471 4477 4593 4602
Size: 151 6 116 9
Fragments arranged by size:
624 425 411 411 286 280 176 151
148 128 122 120 117 117 116 100
99 88 85 70 59 58 53 52
50 48 45 42 41 21 14 13
11 9 6 6
BspEI T'CCGG_A
Cuts at: 0 1811 3144 4602
Size: 1811 1333 1458
Fragments arranged by size:
1811 1458 1333
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 124 829 868 926 1217 2261 2363 2824
Size: 124 705 39 58 291 1044 102 461
Cuts at: 2824 2941 3091 3217 3536 3551 3567 4602
Size: 117 150 126 319 15 16 1035
Fragments arranged by size:
1044 1035 705 461 319 291 150 126
124 117 102 58 39 16 15
BsrBI CCG'CTC
Cuts at: 0 336 1070 1817 2495 4602
Size: 336 734 747 678 2107
Fragments arranged by size:
2107 747 734 678 336
BsrDI GCAATG_nn'
Cuts at: 0 873 2529 2666 3632 4174 4602
Size: 873 1656 137 966 542 428
Fragments arranged by size:
1656 966 873 542 428 137
BsrFI r'CCGG_y
Cuts at: 0 36 2983 3922 4602
Size: 36 2947 939 680
Fragments arranged by size:
2947 939 680 36
BsrGI T'GTAC_A
Cuts at: 0 2840 3611 3950 4602
Size: 2840 771 339 652
Fragments arranged by size:
2840 771 652 339
BssSI C'ACGA_G
Cuts at: 0 2362 3166 4470 4602
Size: 2362 804 1304 132
Fragments arranged by size:
2362 1304 804 132
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1877 4173 4281 4602
Size: 1877 2296 108 321
Fragments arranged by size:
2296 1877 321 108
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1569 1800 3426 3807 4602
Size: 1569 231 1626 381 795
Fragments arranged by size:
1626 1569 795 381 231
BstYI r'GATC_y
Cuts at: 0 224 1247 1598 3147 3356 3602 3908 4602
Size: 224 1023 351 1549 209 246 306 694
Fragments arranged by size:
1549 1023 694 351 306 246 224 209
Bsu36I CC'TnA_GG
Cuts at: 0 4302 4602
Size: 4302 300
BtgI C'CryG_G
Cuts at: 0 128 141 1119 1622 2228 2517 3556 3584
Size: 128 13 978 503 606 289 1039 28
Cuts at: 3584 3806 3872 3930 4143 4161 4602
Size: 222 66 58 213 18 441
Fragments arranged by size:
1039 978 606 503 441 289 222 213
128 66 58 28 18 13
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 2103 4553 4602
Size: 2103 2450 49
Fragments arranged by size:
2450 2103 49
BtsI GCAGTG_nn'
Cuts at: 0 688 899 1222 2467 3246 4086 4282 4290
Size: 688 211 323 1245 779 840 196 8
Cuts at: 4290 4602
Size: 312
Fragments arranged by size:
1245 840 779 688 323 312 211 196
8
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 634 669 2970 3005 4602
Size: 634 35 2301 35 1597
Fragments arranged by size:
2301 1597 634 35 35
DraI TTT'AAA
Cuts at: 0 1683 4602
Size: 1683 2919
DraIII CAC_nnn'GTG
Cuts at: 0 722 4324 4326 4580 4602
Size: 722 3602 2 254 22
Fragments arranged by size:
3602 722 254 22 2
DrdI GACnn_nn'nnGTC
Cuts at: 0 2230 2571 4602
Size: 2230 341 2031
Fragments arranged by size:
2230 2031 341
EaeI y'GGCC_r
Cuts at: 0 43 697 848 911 2006 2582 3553 4146
Size: 43 654 151 63 1095 576 971 593
Cuts at: 4146 4287 4602
Size: 141 315
Fragments arranged by size:
1095 971 654 593 576 315 151 141
63 43
EagI C'GGCC_G
Cuts at: 0 43 4602
Size: 43 4559
EarI CTCTTCn'nnn_
Cuts at: 0 718 766 4602
Size: 718 48 3836
Fragments arranged by size:
3836 718 48
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 619 3886 4602
Size: 619 3267 716
Fragments arranged by size:
3267 716 619
EcoICRI GAG'CTC
Cuts at: 0 51 4602
Size: 51 4551
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 352 568 967 998 1563 1606 1684 1720
Size: 352 216 399 31 565 43 78 36
Cuts at: 1720 1822 1963 2133 4528 4602
Size: 102 141 170 2395 74
Fragments arranged by size:
2395 565 399 352 216 170 141 102
78 74 43 36 31
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1261 2266 2542 4602
Size: 1261 1005 276 2060
Fragments arranged by size:
2060 1261 1005 276
EcoO109I rG'GnC_Cy
Cuts at: 0 23 524 1062 1206 1748 1847 2259 2684
Size: 23 501 538 144 542 99 412 425
Cuts at: 2684 2746 3284 4075 4247 4248 4383 4497 4544
Size: 62 538 791 172 1 135 114 47
Cuts at: 4544 4602
Size: 58
Fragments arranged by size:
791 542 538 538 501 425 412 172
144 135 114 99 62 58 47 23
1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 255 4542 4602
Size: 255 4287 60
Fragments arranged by size:
4287 255 60
FspI TGC'GCA
Cuts at: 0 1363 1909 4599 4602
Size: 1363 546 2690 3
Fragments arranged by size:
2690 1363 546 3
HaeII r_GCGC'y
Cuts at: 0 7 84 195 1413 3850 4602
Size: 7 77 111 1218 2437 752
Fragments arranged by size:
2437 1218 752 111 77 7
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1011 1043 2551 2583 3124 3156 3759 3791
Size: 1011 32 1508 32 541 32 603 32
Cuts at: 3791 3868 3900 4204 4236 4351 4383 4531 4563
Size: 77 32 304 32 115 32 148 32
Cuts at: 4563 4602
Size: 39
Fragments arranged by size:
1508 1011 603 541 304 148 115 77
39 32 32 32 32 32 32 32
32
HincII GTy'rAC
Cuts at: 0 3163 3306 3504 3882 4602
Size: 3163 143 198 378 720
Fragments arranged by size:
3163 720 378 198 143
HindIII A'AGCT_T
Cuts at: 0 452 4602
Size: 452 4150
KasI G'GCGC_C
Cuts at: 0 80 1409 3846 4602
Size: 80 1329 2437 756
Fragments arranged by size:
2437 1329 756 80
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 665 2776 4602
Size: 665 2111 1826
Fragments arranged by size:
2111 1826 665
MscI TGG'CCA
Cuts at: 0 2008 2584 3555 4148 4289 4602
Size: 2008 576 971 593 141 313
Fragments arranged by size:
2008 971 593 576 313 141
MslI CAynn'nnrTG
Cuts at: 0 233 589 1675 2208 2416 2522 3038 3662
Size: 233 356 1086 533 208 106 516 624
Cuts at: 3662 3805 4154 4602
Size: 143 349 448
Fragments arranged by size:
1086 624 533 516 448 356 349 233
208 143 106
MspA1I CmG'CkG
Cuts at: 0 258 273 360 489 509 696 785 1394
Size: 258 15 87 129 20 187 89 609
Cuts at: 1394 1506 1553 1800 1877 2034 2337 2427 2624
Size: 112 47 247 77 157 303 90 197
Cuts at: 2624 2636 2861 2900 3126 3272 3889 4129 4199
Size: 12 225 39 226 146 617 240 70
Cuts at: 4199 4602
Size: 403
Fragments arranged by size:
617 609 403 303 258 247 240 226
225 197 187 157 146 129 112 90
89 87 77 70 47 39 20 15
12
NarI GG'CG_CC
Cuts at: 0 81 1410 3847 4602
Size: 81 1329 2437 755
Fragments arranged by size:
2437 1329 755 81
NcoI C'CATG_G
Cuts at: 0 3806 4143 4161 4602
Size: 3806 337 18 441
Fragments arranged by size:
3806 441 337 18
NdeI CA'TA_TG
Cuts at: 0 608 4602
Size: 608 3994
NspI r_CATG'y
Cuts at: 0 369 1824 3216 4602
Size: 369 1455 1392 1386
Fragments arranged by size:
1455 1392 1386 369
PasI CC'CwG_GG
Cuts at: 0 4072 4602
Size: 4072 530
PciI A'CATG_T
Cuts at: 0 365 4602
Size: 365 4237
PflMI CCAn_nnn'nTGG
Cuts at: 0 2694 3004 3180 3294 4602
Size: 2694 310 176 114 1308
Fragments arranged by size:
2694 1308 310 176 114
PfoI T'CCnGG_A
Cuts at: 0 975 1202 1661 2328 4391 4602
Size: 975 227 459 667 2063 211
Fragments arranged by size:
2063 975 667 459 227 211
PmlI CAC'GTG
Cuts at: 0 2969 4602
Size: 2969 1633
PpuMI rG'GwC_Cy
Cuts at: 0 23 1206 1847 2746 4383 4544 4602
Size: 23 1183 641 899 1637 161 58
Fragments arranged by size:
1637 1183 899 641 161 58 23
PshAI GACnn'nnGTC
Cuts at: 0 2337 4602
Size: 2337 2265
PspOMI G'GGCC_C
Cuts at: 0 729 2258 2683 3196 3284 4075 4247 4602
Size: 729 1529 425 513 88 791 172 355
Fragments arranged by size:
1529 791 729 513 425 355 172 88
PstI C_TGCA'G
Cuts at: 0 158 272 865 1214 2129 2360 2462 3101
Size: 158 114 593 349 915 231 102 639
Cuts at: 3101 3241 3262 3533 3971 4267 4277 4602
Size: 140 21 271 438 296 10 325
Fragments arranged by size:
915 639 593 438 349 325 296 271
231 158 140 114 102 21 10
PvuII CAG'CTG
Cuts at: 0 258 273 360 489 509 785 1506 1800
Size: 258 15 87 129 20 276 721 294
Cuts at: 1800 1877 2034 2337 2427 2636 2900 3126 3889
Size: 77 157 303 90 209 264 226 763
Cuts at: 3889 4199 4602
Size: 310 403
Fragments arranged by size:
763 721 403 310 303 294 276 264
258 226 209 157 129 90 87 77
20 15
RsrII CG'GwC_CG
Cuts at: 0 39 4602
Size: 39 4563
SacI G_AGCT'C
Cuts at: 0 53 4602
Size: 53 4549
SanDI GG'GwC_CC
Cuts at: 0 4383 4602
Size: 4383 219
SapI GCTCTTCn'nnn_
Cuts at: 0 718 4602
Size: 718 3884
SbfI CC_TGCA'GG
Cuts at: 0 2129 4602
Size: 2129 2473
ScaI AGT'ACT
Cuts at: 0 1477 4602
Size: 1477 3125
SexAI A'CCwGG_T
Cuts at: 0 4403 4602
Size: 4403 199
SfcI C'TryA_G
Cuts at: 0 154 268 861 993 1147 1210 2125 2356
Size: 154 114 593 132 154 63 915 231
Cuts at: 2356 2458 3097 3237 3258 3336 3529 3705 3967
Size: 102 639 140 21 78 193 176 262
Cuts at: 3967 4194 4263 4273 4602
Size: 227 69 10 329
Fragments arranged by size:
915 639 593 329 262 231 227 193
176 154 154 140 132 114 102 78
69 63 21 10
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 42 4602
Size: 42 4560
SfoI GGC'GCC
Cuts at: 0 82 1411 3848 4602
Size: 82 1329 2437 754
Fragments arranged by size:
2437 1329 754 82
SmaI CCC'GGG
Cuts at: 0 15 4602
Size: 15 4587
SmlI C'TyrA_G
Cuts at: 0 250 417 1339 1842 2394 2833 4602
Size: 250 167 922 503 552 439 1769
Fragments arranged by size:
1769 922 552 503 439 250 167
SphI G_CATG'C
Cuts at: 0 3216 4602
Size: 3216 1386
StuI AGG'CCT
Cuts at: 0 896 1136 3115 3444 4271 4300 4602
Size: 896 240 1979 329 827 29 302
Fragments arranged by size:
1979 896 827 329 302 240 29
StyI C'CwwG_G
Cuts at: 0 1262 2267 2742 3806 4143 4161 4310 4602
Size: 1262 1005 475 1064 337 18 149 292
Fragments arranged by size:
1262 1064 1005 475 337 292 149 18
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 76 733 2628 4092 4443 4602
Size: 76 657 1895 1464 351 159
Fragments arranged by size:
1895 1464 657 351 159 76
TatI w'GTAC_w
Cuts at: 0 1475 2840 3611 3950 4602
Size: 1475 1365 771 339 652
Fragments arranged by size:
1475 1365 771 652 339
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 116 148 1985 2017 2310 2342 4602
Size: 116 32 1837 32 293 32 2260
Fragments arranged by size:
2260 1837 293 116 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 1531 4387 4602
Size: 1531 2856 215
Fragments arranged by size:
2856 1531 215
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 4093 4210 4602
Size: 4093 117 392
Fragments arranged by size:
4093 392 117
XmaI C'CCGG_G
Cuts at: 0 13 4602
Size: 13 4589
XmnI GAAnn'nnTTC
Cuts at: 0 1318 2314 4602
Size: 1318 996 2288
Fragments arranged by size:
2288 1318 996
Enzymes that do cut:
AarI AccI AcuI AflII AflIII AleI AlfI AloI
AlwNI ApaI ApaLI ApoI AvaI BaeI BamHI BanI
BanII BbeI BbsI BbvCI BcgI BciVI BglI BlpI
Bme1580I BmgBI BmrI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspMI BsrBI BsrDI
BsrFI BsrGI BssSI BstAPI BstXI BstYI Bsu36I BtgI
BtgZI BtsI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I FalI FspI
HaeII Hin4I HincII HindIII KasI MmeI MscI MslI
MspA1I NarI NcoI NdeI NspI PasI PciI PflMI
PfoI PmlI PpuMI PshAI PspOMI PstI PvuII RsrII
SacI SanDI SapI SbfI ScaI SexAI SfcI SfiI
SfoI SmaI SmlI SphI StuI StyI TaqII TatI
TstI Tth111I XcmI XmaI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AgeI AhdI AscI AseI
AsiSI AvrII BclI BfrBI BglII BmtI BplI BsiWI
BspHI BssHII BstBI BstEII BstZ17I ClaI EcoRI EcoRV
FseI FspAI HpaI KpnI MfeI MluI NaeI NgoMIV
NheI NotI NruI NsiI PacI PmeI PpiI PsiI
PspXI PsrI PvuI SacII SalI SgfI SgrAI SnaBI
SpeI SrfI SspI SwaI XbaI XhoI ZraI