(Circular) (Six-base) MAPSORT of: pFN21ASDA0797.seq Check: 420 from: 1 to: 8065 pFN21ASDA0797 8065 bp With 182 enzymes: SgfI * January 16, 2009 15:30 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5875 278 Size: 53 83 186 936 4339 2468 Fragments arranged by size: 4339 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2425 2762 3370 4815 5408 1937 Size: 488 337 608 1445 593 4594 Fragments arranged by size: 4594 1445 608 593 488 337 Acc65I G'GTAC_C Cuts at: 5386 5386 Size: 8065 AclI AA'CG_TT Cuts at: 1563 6192 6565 1563 Size: 4629 373 3063 Fragments arranged by size: 4629 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4139 4425 4803 5086 5270 6128 7512 7780 Size: 2507 286 378 283 184 858 1384 268 Cuts at: 7780 1632 Size: 1917 Fragments arranged by size: 2507 1917 1384 858 378 286 283 268 184 AflII C'TTAA_G Cuts at: 820 1017 4744 820 Size: 197 3727 4141 Fragments arranged by size: 4141 3727 197 AflIII A'CryG_T Cuts at: 1203 2349 6964 7941 1203 Size: 1146 4615 977 1327 Fragments arranged by size: 4615 1327 1146 977 AgeI A'CCGG_T Cuts at: 6896 6896 Size: 8065 AhdI GACnn_n'nnGTC Cuts at: 6794 6794 Size: 8065 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 8065 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3599 1184 Size: 2415 5650 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2400 2432 2400 Size: 32 8033 AlwNI CAG_nnn'CTG Cuts at: 5109 5808 7380 5109 Size: 699 1572 5794 Fragments arranged by size: 5794 1572 699 ApaLI G'TGCA_C Cuts at: 6122 7278 6122 Size: 1156 6909 ApoI r'AATT_y Cuts at: 1493 2270 2797 3643 3768 3911 4061 4140 4640 Size: 777 527 846 125 143 150 79 500 Cuts at: 4640 4989 5182 5374 5600 5752 7861 1493 Size: 349 193 192 226 152 2109 1697 Fragments arranged by size: 2109 1697 846 777 527 500 349 226 193 192 152 150 143 125 79 AseI AT'TA_AT Cuts at: 160 1032 2538 3723 6619 160 Size: 872 1506 1185 2896 1606 Fragments arranged by size: 2896 1606 1506 1185 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8065 AvaI C'yCGr_G Cuts at: 1870 1943 5390 1870 Size: 73 3447 4545 Fragments arranged by size: 4545 3447 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2641 2674 2641 Size: 33 8032 BamHI G'GATC_C Cuts at: 5395 5762 5395 Size: 367 7698 BanI G'GyrC_C Cuts at: 618 943 1160 1787 5386 6841 618 Size: 325 217 627 3599 1455 1842 Fragments arranged by size: 3599 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 5072 5384 1391 Size: 3681 312 4072 Fragments arranged by size: 4072 3681 312 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2288 3198 4134 928 Size: 971 389 910 936 4859 Fragments arranged by size: 4859 971 936 910 389 BbvCI CC'TCA_GC Cuts at: 3081 3081 Size: 8065 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 6256 6290 1903 Size: 34 4319 34 3678 Fragments arranged by size: 4319 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4949 5957 7167 4949 Size: 1008 1210 5847 Fragments arranged by size: 5847 1210 1008 BclI T'GATC_A Cuts at: 1548 2131 1548 Size: 583 7482 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 6676 136 Size: 107 122 71 631 605 5004 1525 Fragments arranged by size: 5004 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 8060 8060 Size: 8065 BlpI GC'TnA_GC Cuts at: 3572 5482 3572 Size: 1910 6155 Bme1580I G_kGCm'C Cuts at: 1790 6126 7282 1790 Size: 4336 1156 2573 Fragments arranged by size: 4336 2573 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 8065 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3965 5796 6754 Size: 416 53 83 399 24 2548 1831 958 Cuts at: 6754 442 Size: 1753 Fragments arranged by size: 2548 1831 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 8065 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2823 3291 3963 3987 6725 1239 Size: 1584 468 672 24 2738 2579 Fragments arranged by size: 2738 2579 1584 672 468 24 Bpu10I CC'TnA_GC Cuts at: 3081 4008 3081 Size: 927 7138 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3282 6190 7055 7353 3282 Size: 2908 865 298 3994 Fragments arranged by size: 3994 2908 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 6728 882 Size: 617 5229 2219 Fragments arranged by size: 5229 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4264 5362 5856 493 Size: 711 3060 1098 494 2702 Fragments arranged by size: 3060 2702 1098 711 494 BsaBI GATnn'nnATC Cuts at: 4539 8059 4539 Size: 3520 4545 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 5872 6254 6889 275 Size: 53 83 186 936 4339 382 635 1451 Fragments arranged by size: 4339 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 6498 6896 7170 7317 7728 6498 Size: 398 274 147 411 6835 Fragments arranged by size: 6835 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 7840 Fragments arranged by size: 7840 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2664 2667 3069 4204 1186 Size: 1478 3 402 1135 5047 Fragments arranged by size: 5047 1478 1135 402 3 BseYI C'CCAG_C Cuts at: 7268 7764 7268 Size: 496 7569 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3096 4025 4296 1163 Size: 573 1360 929 271 4932 Fragments arranged by size: 4932 1360 929 573 271 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2031 5515 6276 7304 1540 Size: 275 186 30 3484 761 1028 2301 Fragments arranged by size: 3484 2301 1028 761 275 186 30 BsiHKAI G_wGCw'C Cuts at: 6126 6211 7282 6126 Size: 85 1071 6909 Fragments arranged by size: 6909 1071 85 BsmI GAATG_Cn' Cuts at: 3183 5576 5669 3183 Size: 2393 93 5579 Fragments arranged by size: 5579 2393 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 5072 5384 6126 6211 7282 1391 Size: 399 3282 312 742 85 1071 2174 Fragments arranged by size: 3282 2174 1071 742 399 312 85 BspEI T'CCGG_A Cuts at: 7728 7728 Size: 8065 BspHI T'CATG_A Cuts at: 2134 4198 5954 7684 2134 Size: 2064 1756 1730 2515 Fragments arranged by size: 2515 2064 1756 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4047 5420 844 Size: 3203 1373 3489 Fragments arranged by size: 3489 3203 1373 BsrBI CCG'CTC Cuts at: 1394 1823 2040 5952 1394 Size: 429 217 3912 3507 Fragments arranged by size: 3912 3507 429 217 BsrDI GCAATG_nn' Cuts at: 65 1248 3215 4516 6560 6734 65 Size: 1183 1967 1301 2044 174 1396 Fragments arranged by size: 2044 1967 1396 1301 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2030 6709 6896 1700 Size: 110 220 4679 187 2869 Fragments arranged by size: 4679 2869 220 187 110 BsrGI T'GTAC_A Cuts at: 96 3806 96 Size: 3710 4355 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 8065 BssSI C'ACGA_G Cuts at: 6125 7137 6125 Size: 1012 7053 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 8065 BstBI TT'CG_AA Cuts at: 2007 6869 2007 Size: 4862 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 8065 BstXI CCAn_nnnn'nTGG Cuts at: 4453 4453 Size: 8065 BstYI r'GATC_y Cuts at: 1972 5395 5762 6147 6164 7616 7702 7714 8060 Size: 3423 367 385 17 1452 86 12 346 Cuts at: 8060 1972 Size: 1977 Fragments arranged by size: 3423 1977 1452 385 367 346 86 17 12 BstZ17I GTA'TAC Cuts at: 3371 3371 Size: 8065 BtgI C'CryG_G Cuts at: 513 1065 2022 513 Size: 552 957 6556 Fragments arranged by size: 6556 957 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 4785 487 Size: 683 56 3559 3767 Fragments arranged by size: 3767 3559 683 56 BtsI GCAGTG_nn' Cuts at: 4594 4858 5581 6375 6395 4594 Size: 264 723 794 20 6264 Fragments arranged by size: 6264 794 723 264 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 8065 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 8030 DraI TTT'AAA Cuts at: 2113 2317 3357 4068 4911 5369 2113 Size: 204 1040 711 843 458 4809 Fragments arranged by size: 4809 1040 843 711 458 204 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 7072 809 Size: 507 5756 1802 Fragments arranged by size: 5756 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2028 5512 6401 Size: 54 1425 50 275 18 198 3484 889 Cuts at: 6401 8030 8 Size: 1629 43 Fragments arranged by size: 3484 1629 1425 889 275 198 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2028 5512 1537 Size: 275 216 3484 4090 Fragments arranged by size: 4090 3484 275 216 EarI CTCTTCn'nnn_ Cuts at: 1353 2589 3058 3392 3816 4480 5995 1353 Size: 1236 469 334 424 664 1515 3423 Fragments arranged by size: 3423 1515 1236 664 469 424 334 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 6652 7026 7172 387 Size: 1064 138 5063 374 146 1280 Fragments arranged by size: 5063 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2823 3291 3963 3987 4139 4425 4803 Size: 393 1191 468 672 24 152 286 378 Cuts at: 4803 5086 5270 6128 6725 7512 7780 1239 Size: 283 184 858 597 787 268 1524 Fragments arranged by size: 1524 1191 858 787 672 597 468 393 378 286 283 268 184 152 24 EcoO109I rG'GnC_Cy Cuts at: 4036 4964 4036 Size: 928 7137 EcoRI G'AATT_C Cuts at: 2270 2797 4140 4640 5374 5752 2270 Size: 527 1343 500 734 378 4583 Fragments arranged by size: 4583 1343 734 527 500 378 FspI TGC'GCA Cuts at: 6571 6571 Size: 8065 HaeII r_GCGC'y Cuts at: 1678 7212 1678 Size: 5534 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2116 2148 2588 2620 3976 Size: 32 135 32 544 32 440 32 1356 Cuts at: 3976 4008 4180 4212 5217 5249 5285 5317 6706 Size: 32 172 32 1005 32 36 32 1389 Cuts at: 6706 6738 6780 6812 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1389 1356 1005 544 440 172 135 42 36 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4816 5409 5655 669 Size: 988 281 2878 593 246 3079 Fragments arranged by size: 3079 2878 988 593 281 246 HindIII A'AGCT_T Cuts at: 748 2073 748 Size: 1325 6740 HpaI GTT'AAC Cuts at: 5655 5655 Size: 8065 KpnI G_GTAC'C Cuts at: 5390 5390 Size: 8065 MfeI C'AATT_G Cuts at: 2337 2874 5664 2337 Size: 537 2790 4738 Fragments arranged by size: 4738 2790 537 MluI A'CGCG_T Cuts at: 7941 7941 Size: 8065 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3604 7179 7363 1305 Size: 2299 3575 184 2007 Fragments arranged by size: 3575 2299 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 6243 Fragments arranged by size: 6243 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2442 4383 4389 4737 6023 6382 6541 Size: 648 1276 1941 6 348 1286 359 159 Cuts at: 6541 518 Size: 2042 Fragments arranged by size: 2042 1941 1286 1276 648 359 348 159 6 MspA1I CmG'CkG Cuts at: 1606 5481 6158 7306 7551 1606 Size: 3875 677 1148 245 2120 Fragments arranged by size: 3875 2120 1148 677 245 NaeI GCC'GGC Cuts at: 2032 2032 Size: 8065 NcoI C'CATG_G Cuts at: 513 1065 2022 513 Size: 552 957 6556 Fragments arranged by size: 6556 957 552 NdeI CA'TA_TG Cuts at: 387 2287 4583 387 Size: 1900 2296 3869 Fragments arranged by size: 3869 2296 1900 NgoMIV G'CCGG_C Cuts at: 2030 2030 Size: 8065 NheI G'CTAG_C Cuts at: 1052 1052 Size: 8065 NotI GC'GGCC_GC Cuts at: 5512 5512 Size: 8065 NspI r_CATG'y Cuts at: 1132 1229 2353 3102 5423 6968 1132 Size: 97 1124 749 2321 1545 2229 Fragments arranged by size: 2321 2229 1545 1124 749 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 8065 PciI A'CATG_T Cuts at: 2349 6964 2349 Size: 4615 3450 PflMI CCAn_nnn'nTGG Cuts at: 1108 3122 1108 Size: 2014 6051 PmeI GTTT'AAAC Cuts at: 5369 5369 Size: 8065 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 6134 6166 7647 7679 6134 Size: 32 1481 32 6520 Fragments arranged by size: 6520 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 4036 4036 Size: 8065 PshAI GACnn'nnGTC Cuts at: 1613 2812 1613 Size: 1199 6866 PsiI TTA'TAA Cuts at: 4572 5635 4572 Size: 1063 7002 PstI C_TGCA'G Cuts at: 830 4886 5417 830 Size: 4056 531 3478 Fragments arranged by size: 4056 3478 531 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8065 SalI G'TCGA_C Cuts at: 1936 4814 5407 1936 Size: 2878 593 4594 Fragments arranged by size: 4594 2878 593 SbfI CC_TGCA'GG Cuts at: 5417 5417 Size: 8065 ScaI AGT'ACT Cuts at: 6313 8025 6313 Size: 1712 6353 SfcI C'TryA_G Cuts at: 826 1045 2412 2653 3465 3531 4416 4703 4765 Size: 219 1367 241 812 66 885 287 62 Cuts at: 4765 4882 5255 5413 6548 7229 7420 826 Size: 117 373 158 1135 681 191 1471 Fragments arranged by size: 1471 1367 1135 885 812 681 373 287 241 219 191 158 117 66 62 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8065 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 8065 SmaI CCC'GGG Cuts at: 1872 5392 1872 Size: 3520 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3297 4744 6169 7070 7332 820 Size: 197 926 1354 1447 1425 901 262 1553 Fragments arranged by size: 1553 1447 1425 1354 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 5362 5856 493 Size: 4869 494 2702 Fragments arranged by size: 4869 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 8065 SphI G_CATG'C Cuts at: 1132 5423 1132 Size: 4291 3774 SspI AAT'ATT Cuts at: 5 52 5989 5 Size: 47 5937 2081 Fragments arranged by size: 5937 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 8065 StyI C'CwwG_G Cuts at: 513 1065 2022 2563 5504 513 Size: 552 957 541 2941 3074 Fragments arranged by size: 3074 2941 957 552 541 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2505 4152 4962 6092 6109 6262 6447 Size: 1255 925 1647 810 1130 17 153 185 Cuts at: 6447 6892 325 Size: 445 1498 Fragments arranged by size: 1647 1498 1255 1130 925 810 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2635 3806 6311 Size: 274 80 33 51 1443 658 1171 2505 Cuts at: 6311 8023 96 Size: 1712 138 Fragments arranged by size: 2505 1712 1443 1171 658 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2505 2537 4675 4707 8025 8057 2505 Size: 32 2138 32 3318 32 2513 Fragments arranged by size: 3318 2513 2138 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3347 1316 Size: 2031 6034 XbaI T'CTAG_A Cuts at: 5401 5401 Size: 8065 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 8065 XmaI C'CCGG_G Cuts at: 1870 5390 1870 Size: 3520 4545 XmnI GAAnn'nnTTC Cuts at: 2868 5047 6194 2868 Size: 2179 1147 4739 Fragments arranged by size: 4739 2179 1147 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5873 276 Size: 53 83 186 936 4339 2468 Fragments arranged by size: 4339 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BbeI BfrBI BplI BsiWI BsmBI Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI