(Circular) (Six-base) MAPSORT of: pFN21ASDA0782.seq Check: 9998 from: 1 to: 8344 pFN21ASDA0782 8344 bp With 182 enzymes: SgfI * January 16, 2009 15:30 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2364 5363 2364 Size: 2999 5345 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 6154 278 Size: 53 83 186 936 4618 2468 Fragments arranged by size: 4618 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2885 5319 5637 5687 1937 Size: 948 2434 318 50 4594 Fragments arranged by size: 4594 2434 948 318 50 Acc65I G'GTAC_C Cuts at: 4485 5409 5665 4485 Size: 924 256 7164 Fragments arranged by size: 7164 924 256 AclI AA'CG_TT Cuts at: 1563 6471 6844 1563 Size: 4908 373 3063 Fragments arranged by size: 4908 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2791 3077 6407 7791 8059 1632 Size: 1159 286 3330 1384 268 1917 Fragments arranged by size: 3330 1917 1384 1159 286 268 AfeI AGC'GCT Cuts at: 2396 2575 3002 4385 2396 Size: 179 427 1383 6355 Fragments arranged by size: 6355 1383 427 179 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 8147 AflIII A'CryG_T Cuts at: 1203 5506 7243 8220 1203 Size: 4303 1737 977 1327 Fragments arranged by size: 4303 1737 1327 977 AgeI A'CCGG_T Cuts at: 7175 7175 Size: 8344 AhdI GACnn_n'nnGTC Cuts at: 4165 5617 7073 4165 Size: 1452 1456 5436 Fragments arranged by size: 5436 1456 1452 AleI CACnn'nnGTG Cuts at: 1166 3455 5372 1166 Size: 2289 1917 4138 Fragments arranged by size: 4138 2289 1917 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 5234 1184 Size: 4050 4294 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3920 3952 3920 Size: 32 8312 AlwNI CAG_nnn'CTG Cuts at: 2527 2934 3418 4054 4297 4847 5214 5525 6087 Size: 407 484 636 243 550 367 311 562 Cuts at: 6087 7659 2527 Size: 1572 3212 Fragments arranged by size: 3212 1572 636 562 550 484 407 367 311 243 ApaI G_GGCC'C Cuts at: 2563 3583 3925 3984 4623 5266 2563 Size: 1020 342 59 639 643 5641 Fragments arranged by size: 5641 1020 643 639 342 59 ApaLI G'TGCA_C Cuts at: 4331 4595 6401 7557 4331 Size: 264 1806 1156 5118 Fragments arranged by size: 5118 1806 1156 264 ApoI r'AATT_y Cuts at: 1493 3428 5653 5879 6031 8140 1493 Size: 1935 2225 226 152 2109 1697 Fragments arranged by size: 2225 2109 1935 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 6898 160 Size: 872 5866 1606 Fragments arranged by size: 5866 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8344 AvaI C'yCGr_G Cuts at: 1870 1943 2556 3361 3582 3864 5514 5669 1870 Size: 73 613 805 221 282 1650 155 4545 Fragments arranged by size: 4545 1650 805 613 282 221 155 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 3752 3785 3752 Size: 33 8311 BamHI G'GATC_C Cuts at: 5674 6041 5674 Size: 367 7977 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3198 3457 4485 5409 5665 Size: 325 217 627 1411 259 1028 924 256 Cuts at: 5665 7120 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 1411 1028 924 627 325 259 256 217 BanII G_rGCy'C Cuts at: 1391 2043 2252 2563 2953 3099 3583 3925 3960 Size: 652 209 311 390 146 484 342 35 Cuts at: 3960 3984 4413 4623 4725 4893 5266 5439 5502 Size: 24 429 210 102 168 373 173 63 Cuts at: 5502 5663 1391 Size: 161 4072 Fragments arranged by size: 4072 652 484 429 390 373 342 311 210 209 173 168 161 146 102 63 35 24 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3961 4292 4680 928 Size: 971 2062 331 388 4592 Fragments arranged by size: 4592 2062 971 388 331 BbvCI CC'TCA_GC Cuts at: 2874 2955 4884 2874 Size: 81 1929 6334 Fragments arranged by size: 6334 1929 81 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 6535 6569 1903 Size: 34 4598 34 3678 Fragments arranged by size: 4598 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2338 6236 7446 2338 Size: 3898 1210 3236 Fragments arranged by size: 3898 3236 1210 BclI T'GATC_A Cuts at: 1548 2350 2457 1548 Size: 802 107 7435 Fragments arranged by size: 7435 802 107 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2486 3528 4408 Size: 107 122 71 631 605 814 1042 880 Cuts at: 4408 5075 5157 6955 136 Size: 667 82 1798 1525 Fragments arranged by size: 1798 1525 1042 880 814 667 631 605 122 107 82 71 BglII A'GATC_T Cuts at: 8339 8339 Size: 8344 BlpI GC'TnA_GC Cuts at: 2087 2544 5761 2087 Size: 457 3217 4670 Fragments arranged by size: 4670 3217 457 Bme1580I G_kGCm'C Cuts at: 1790 2557 2563 3153 3583 3708 3925 3984 4335 Size: 767 6 590 430 125 217 59 351 Cuts at: 4335 4599 4623 5036 5266 6405 7561 1790 Size: 264 24 413 230 1139 1156 2573 Fragments arranged by size: 2573 1156 1139 767 590 430 413 351 264 230 217 125 59 24 6 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 8344 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2974 3150 6075 Size: 416 53 83 399 24 1557 176 2925 Cuts at: 6075 7033 442 Size: 958 1753 Fragments arranged by size: 2925 1753 1557 958 416 399 176 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 8344 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4495 4495 Size: 8344 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2648 3227 3803 4322 4463 4465 4919 7004 Size: 1409 579 576 519 141 2 454 2085 Cuts at: 7004 1239 Size: 2579 Fragments arranged by size: 2579 2085 1409 579 576 519 454 141 2 Bpu10I CC'TnA_GC Cuts at: 2189 2874 2955 3629 3825 3908 4724 4884 2189 Size: 685 81 674 196 83 816 160 5649 Fragments arranged by size: 5649 816 685 674 196 160 83 81 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4322 4364 6469 7334 7632 4322 Size: 42 2105 865 298 5034 Fragments arranged by size: 5034 2105 865 298 42 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3311 4483 5264 7007 882 Size: 617 1812 1172 781 1743 2219 Fragments arranged by size: 2219 1812 1743 1172 781 617 BsaAI yAC'GTr Cuts at: 493 1204 2425 2659 4357 5641 6135 493 Size: 711 1221 234 1698 1284 494 2702 Fragments arranged by size: 2702 1698 1284 1221 711 494 234 BsaBI GATnn'nnATC Cuts at: 2349 8338 2349 Size: 5989 2355 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2157 3036 3886 6151 Size: 53 83 186 936 624 879 850 2265 Cuts at: 6151 6533 7168 275 Size: 382 635 1451 Fragments arranged by size: 2265 1451 936 879 850 635 624 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3444 5238 6777 7175 7449 7596 8007 3444 Size: 1794 1539 398 274 147 411 3781 Fragments arranged by size: 3781 1794 1539 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2831 2861 2954 2984 3200 Size: 30 165 30 1428 30 93 30 216 Cuts at: 3200 3230 3920 3950 1178 Size: 30 690 30 5572 Fragments arranged by size: 5572 1428 690 216 165 93 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2051 2717 2943 3281 4481 4484 4754 4901 Size: 865 666 226 338 1200 3 270 147 Cuts at: 4901 4988 1186 Size: 87 4542 Fragments arranged by size: 4542 1200 865 666 338 270 226 147 87 3 BseYI C'CCAG_C Cuts at: 2204 2584 3109 3122 3173 3185 3344 3880 4123 Size: 380 525 13 51 12 159 536 243 Cuts at: 4123 4425 4791 4867 5029 5358 5536 7547 8043 Size: 302 366 76 162 329 178 2011 496 Cuts at: 8043 2204 Size: 2505 Fragments arranged by size: 2505 2011 536 525 496 380 366 329 302 243 178 162 159 76 51 13 12 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2702 3027 4798 4976 5006 5489 1163 Size: 573 966 325 1771 178 30 483 4018 Fragments arranged by size: 4018 1771 966 573 483 325 178 30 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3516 5191 5794 6555 7583 1540 Size: 275 186 1515 1675 603 761 1028 2301 Fragments arranged by size: 2301 1675 1515 1028 761 603 275 186 BsiHKAI G_wGCw'C Cuts at: 3018 3099 3735 3960 4335 4599 4725 4893 5439 Size: 81 636 225 375 264 126 168 546 Cuts at: 5439 6405 6490 7561 3018 Size: 966 85 1071 3801 Fragments arranged by size: 3801 1071 966 636 546 375 264 225 168 126 85 81 BsmI GAATG_Cn' Cuts at: 3830 5855 5948 3830 Size: 2025 93 6226 Fragments arranged by size: 6226 2025 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3043 3043 Size: 8344 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2043 2252 2557 2563 2953 3018 3099 Size: 399 253 209 305 6 390 65 81 Cuts at: 3099 3153 3583 3708 3735 3925 3960 3984 4335 Size: 54 430 125 27 190 35 24 351 Cuts at: 4335 4413 4599 4623 4725 4893 5036 5266 5439 Size: 78 186 24 102 168 143 230 173 Cuts at: 5439 5502 5663 6405 6490 7561 1391 Size: 63 161 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 430 399 390 351 305 253 230 209 190 186 173 168 161 143 125 102 85 81 78 65 63 54 35 27 24 24 6 BspEI T'CCGG_A Cuts at: 5238 8007 5238 Size: 2769 5575 BspHI T'CATG_A Cuts at: 3802 6233 7963 3802 Size: 2431 1730 4183 Fragments arranged by size: 4183 2431 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2364 4407 4958 5363 5699 844 Size: 1520 2043 551 405 336 3489 Fragments arranged by size: 3489 2043 1520 551 405 336 BsrBI CCG'CTC Cuts at: 1394 1823 2506 3250 3649 3877 4099 5259 6231 Size: 429 683 744 399 228 222 1160 972 Cuts at: 6231 1394 Size: 3507 Fragments arranged by size: 3507 1160 972 744 683 429 399 228 222 BsrDI GCAATG_nn' Cuts at: 65 1248 6839 7013 65 Size: 1183 5591 174 1396 Fragments arranged by size: 5591 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2769 3554 3597 4799 5280 6988 7175 Size: 110 959 785 43 1202 481 1708 187 Cuts at: 7175 1700 Size: 2869 Fragments arranged by size: 2869 1708 1202 959 785 481 187 110 43 BsrGI T'GTAC_A Cuts at: 96 3747 4083 96 Size: 3651 336 4357 Fragments arranged by size: 4357 3651 336 BssHII G'CGCG_C Cuts at: 1925 4322 1925 Size: 2397 5947 BssSI C'ACGA_G Cuts at: 6404 7416 6404 Size: 1012 7332 BstAPI GCAn_nnn'nTGC Cuts at: 794 2527 3418 794 Size: 1733 891 5720 Fragments arranged by size: 5720 1733 891 BstBI TT'CG_AA Cuts at: 2007 7148 2007 Size: 5141 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 8344 BstXI CCAn_nnnn'nTGG Cuts at: 3310 3388 4640 3310 Size: 78 1252 7014 Fragments arranged by size: 7014 1252 78 BstYI r'GATC_y Cuts at: 1972 2309 3853 4902 5674 6041 6426 6443 7895 Size: 337 1544 1049 772 367 385 17 1452 Cuts at: 7895 7981 7993 8339 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1544 1452 1049 772 385 367 346 337 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3372 5173 5526 3372 Size: 1801 353 6190 Fragments arranged by size: 6190 1801 353 BtgI C'CryG_G Cuts at: 513 1065 2060 2229 2538 2664 3019 3721 5070 Size: 552 995 169 309 126 355 702 1349 Cuts at: 5070 5252 5607 513 Size: 182 355 3250 Fragments arranged by size: 3250 1349 995 702 552 355 355 309 182 169 126 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2854 487 Size: 683 56 1628 5977 Fragments arranged by size: 5977 1628 683 56 BtsI GCAGTG_nn' Cuts at: 2849 3660 3708 4241 4746 4776 5411 5526 5860 Size: 811 48 533 505 30 635 115 334 Cuts at: 5860 6654 6674 2849 Size: 794 20 4519 Fragments arranged by size: 4519 811 794 635 533 505 334 115 48 30 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 8344 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 8309 DraI TTT'AAA Cuts at: 5648 5648 Size: 8344 DraIII CAC_nnn'GTG Cuts at: 4236 4693 5050 4236 Size: 457 357 7530 Fragments arranged by size: 7530 457 357 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 7351 809 Size: 507 6035 1802 Fragments arranged by size: 6035 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2076 2661 2898 Size: 54 1425 50 275 18 246 585 237 Cuts at: 2898 4660 5094 5791 6680 8309 8 Size: 1762 434 697 889 1629 43 Fragments arranged by size: 1762 1629 1425 889 697 585 434 275 246 237 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5791 1537 Size: 275 3979 4090 Fragments arranged by size: 4090 3979 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2127 3104 3582 3984 4418 4634 4713 4824 Size: 774 977 478 402 434 216 79 111 Cuts at: 4824 5628 6274 1353 Size: 804 646 3423 Fragments arranged by size: 3423 977 804 774 646 478 434 402 216 111 79 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2184 6931 7305 7451 387 Size: 1064 138 595 4747 374 146 1280 Fragments arranged by size: 4747 1280 1064 595 374 146 138 EcoICRI GAG'CTC Cuts at: 3097 3958 4723 4891 5437 3097 Size: 861 765 168 546 6004 Fragments arranged by size: 6004 861 765 546 168 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2648 2791 3077 3227 3803 4322 4463 Size: 393 1016 143 286 150 576 519 141 Cuts at: 4463 4465 4919 6407 7004 7791 8059 1239 Size: 2 454 1488 597 787 268 1524 Fragments arranged by size: 1524 1488 1016 787 597 576 519 454 393 286 268 150 143 141 2 EcoNI CCTnn'n_nnAGG Cuts at: 2531 3848 4313 4340 2531 Size: 1317 465 27 6535 Fragments arranged by size: 6535 1317 465 27 EcoO109I rG'GnC_Cy Cuts at: 2524 2878 3163 3280 3367 3376 3625 3980 3981 Size: 354 285 117 87 9 249 355 1 Cuts at: 3981 4537 4788 4864 5262 5263 2524 Size: 556 251 76 398 1 5605 Fragments arranged by size: 5605 556 398 355 354 285 251 249 117 87 76 9 1 1 EcoRI G'AATT_C Cuts at: 3428 5653 6031 3428 Size: 2225 378 5741 Fragments arranged by size: 5741 2225 378 EcoRV GAT'ATC Cuts at: 4195 4195 Size: 8344 FspI TGC'GCA Cuts at: 5630 6850 5630 Size: 1220 7124 HaeII r_GCGC'y Cuts at: 1678 2398 2577 3004 3033 3123 3329 3604 4379 Size: 720 179 427 29 90 206 275 775 Cuts at: 4379 4387 4432 4987 7491 1678 Size: 8 45 555 2504 2531 Fragments arranged by size: 2531 2504 775 720 555 427 275 206 179 90 45 29 8 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2090 2122 2301 2333 2372 Size: 32 135 32 518 32 179 32 39 Cuts at: 2372 2404 2867 2899 2954 2986 4151 4183 4310 Size: 32 463 32 55 32 1165 32 127 Cuts at: 4310 4342 4355 4387 4894 4926 5603 5635 6985 Size: 32 13 32 507 32 677 32 1350 Cuts at: 6985 7017 7059 7091 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1350 1165 677 518 507 463 179 135 127 55 42 39 32 32 32 32 32 32 32 32 32 32 32 32 32 32 13 HincII GTy'rAC Cuts at: 669 1657 1938 4519 4963 5688 5934 669 Size: 988 281 2581 444 725 246 3079 Fragments arranged by size: 3079 2581 988 725 444 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 8344 HpaI GTT'AAC Cuts at: 5934 5934 Size: 8344 KpnI G_GTAC'C Cuts at: 4489 5413 5669 4489 Size: 924 256 7164 Fragments arranged by size: 7164 924 256 MfeI C'AATT_G Cuts at: 4608 5943 4608 Size: 1335 7009 MluI A'CGCG_T Cuts at: 5506 8220 5506 Size: 2714 5630 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2829 4149 7458 7642 1305 Size: 1524 1320 3309 184 2007 Fragments arranged by size: 3309 2007 1524 1320 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2078 5096 10 Size: 54 1425 343 246 3018 3258 Fragments arranged by size: 3258 3018 1425 343 246 54 MslI CAynn'nnrTG Cuts at: 518 1166 2148 2738 2744 3455 4359 4800 5372 Size: 648 982 590 6 711 904 441 572 Cuts at: 5372 5429 6302 6661 6820 518 Size: 57 873 359 159 2042 Fragments arranged by size: 2042 982 904 873 711 648 590 572 441 359 159 57 6 MspA1I CmG'CkG Cuts at: 1606 3109 3919 3997 4215 4294 4735 5214 5609 Size: 1503 810 78 218 79 441 479 395 Cuts at: 5609 5760 6437 7585 7830 1606 Size: 151 677 1148 245 2120 Fragments arranged by size: 2120 1503 1148 810 677 479 441 395 245 218 151 79 78 NaeI GCC'GGC Cuts at: 3556 3599 3556 Size: 43 8301 NcoI C'CATG_G Cuts at: 513 1065 2229 2538 3721 5070 513 Size: 552 1164 309 1183 1349 3787 Fragments arranged by size: 3787 1349 1183 1164 552 309 NdeI CA'TA_TG Cuts at: 387 387 Size: 8344 NgoMIV G'CCGG_C Cuts at: 3554 3597 3554 Size: 43 8301 NheI G'CTAG_C Cuts at: 1052 1052 Size: 8344 NotI GC'GGCC_GC Cuts at: 5791 5791 Size: 8344 NspI r_CATG'y Cuts at: 1132 1229 4799 5702 7247 1132 Size: 97 3570 903 1545 2229 Fragments arranged by size: 3570 2229 1545 903 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 8344 PciI A'CATG_T Cuts at: 7243 7243 Size: 8344 PflMI CCAn_nnn'nTGG Cuts at: 1108 4054 4693 5050 5071 5358 1108 Size: 2946 639 357 21 287 4094 Fragments arranged by size: 4094 2946 639 357 287 21 PfoI T'CCnGG_A Cuts at: 3972 4905 5439 3972 Size: 933 534 6877 Fragments arranged by size: 6877 933 534 PmeI GTTT'AAAC Cuts at: 5648 5648 Size: 8344 PmlI CAC'GTG Cuts at: 2425 4357 2425 Size: 1932 6412 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3920 3952 6413 6445 7926 7958 3920 Size: 32 2461 32 1481 32 4306 Fragments arranged by size: 4306 2461 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 3367 3625 4788 4864 3367 Size: 258 1163 76 6847 Fragments arranged by size: 6847 1163 258 76 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 8344 PsiI TTA'TAA Cuts at: 5914 5914 Size: 8344 PspOMI G'GGCC_C Cuts at: 2559 3579 3921 3980 4619 5262 2559 Size: 1020 342 59 639 643 5641 Fragments arranged by size: 5641 1020 643 639 342 59 PstI C_TGCA'G Cuts at: 830 2532 2920 3171 3295 3423 4017 5696 830 Size: 1702 388 251 124 128 594 1679 3478 Fragments arranged by size: 3478 1702 1679 594 388 251 128 124 PvuI CG_AT'CG Cuts at: 2001 5191 2001 Size: 3190 5154 PvuII CAG'CTG Cuts at: 3109 5214 3109 Size: 2105 6239 SacI G_AGCT'C Cuts at: 3099 3960 4725 4893 5439 3099 Size: 861 765 168 546 6004 Fragments arranged by size: 6004 861 765 546 168 SacII CC_GC'GG Cuts at: 5610 5610 Size: 8344 SalI G'TCGA_C Cuts at: 1936 5686 1936 Size: 3750 4594 SanDI GG'GwC_CC Cuts at: 3367 4788 3367 Size: 1421 6923 SapI GCTCTTCn'nnn_ Cuts at: 3104 3984 4418 4634 4713 3104 Size: 880 434 216 79 6735 Fragments arranged by size: 6735 880 434 216 79 SbfI CC_TGCA'GG Cuts at: 5696 5696 Size: 8344 ScaI AGT'ACT Cuts at: 6592 8304 6592 Size: 1712 6632 SexAI A'CCwGG_T Cuts at: 4047 5046 4047 Size: 999 7345 SfcI C'TryA_G Cuts at: 826 1045 2528 2916 3167 3291 3419 3566 3914 Size: 219 1483 388 251 124 128 147 348 Cuts at: 3914 4013 5692 6827 7508 7699 826 Size: 99 1679 1135 681 191 1471 Fragments arranged by size: 1679 1483 1471 1135 681 388 348 251 219 191 147 128 124 99 SfiI GGCCn_nnn'nGGCC Cuts at: 5075 5075 Size: 8344 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8344 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 8344 SmaI CCC'GGG Cuts at: 1872 2558 3584 3866 5516 5671 1872 Size: 686 1026 282 1650 155 4545 Fragments arranged by size: 4545 1650 1026 686 282 155 SmlI C'TyrA_G Cuts at: 820 1017 1943 4337 4379 6448 7349 7611 820 Size: 197 926 2394 42 2069 901 262 1553 Fragments arranged by size: 2394 2069 1553 926 901 262 197 42 SnaBI TAC'GTA Cuts at: 493 5641 6135 493 Size: 5148 494 2702 Fragments arranged by size: 5148 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 8344 SphI G_CATG'C Cuts at: 1132 4799 5702 1132 Size: 3667 903 3774 Fragments arranged by size: 3774 3667 903 SrfI GCCC'GGGC Cuts at: 2558 2558 Size: 8344 SspI AAT'ATT Cuts at: 5 52 6268 5 Size: 47 6216 2081 Fragments arranged by size: 6216 2081 47 StuI AGG'CCT Cuts at: 1517 4448 1517 Size: 2931 5413 StyI C'CwwG_G Cuts at: 513 1065 2119 2229 2538 3042 3613 3721 5070 Size: 552 1054 110 309 504 571 108 1349 Cuts at: 5070 5783 513 Size: 713 3074 Fragments arranged by size: 3074 1349 1054 713 571 552 504 309 110 108 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2323 3734 5365 6371 6388 6541 6726 Size: 1255 743 1411 1631 1006 17 153 185 Cuts at: 6726 7171 325 Size: 445 1498 Fragments arranged by size: 1631 1498 1411 1255 1006 743 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3747 4083 4554 Size: 274 80 33 51 1443 1770 336 471 Cuts at: 4554 6590 8302 96 Size: 2036 1712 138 Fragments arranged by size: 2036 1770 1712 1443 471 336 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 8304 8336 8304 Size: 32 8312 Tth111I GACn'n_nGTC Cuts at: 1316 3623 1316 Size: 2307 6037 XbaI T'CTAG_A Cuts at: 2698 5680 2698 Size: 2982 5362 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2717 3251 3307 5355 2717 Size: 534 56 2048 5706 Fragments arranged by size: 5706 2048 534 56 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 8344 XmaI C'CCGG_G Cuts at: 1870 2556 3582 3864 5514 5669 1870 Size: 686 1026 282 1650 155 4545 Fragments arranged by size: 4545 1650 1026 686 282 155 XmnI GAAnn'nnTTC Cuts at: 3131 3432 6473 3131 Size: 301 3041 5002 Fragments arranged by size: 5002 3041 301 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 6152 276 Size: 53 83 186 936 4618 2468 Fragments arranged by size: 4618 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AscI AvrII BbeI BfrBI BsiWI BstZ17I FalI FseI FspAI KasI NarI NruI NsiI PacI PspXI PsrI RsrII SfoI SwaI