(Circular) (Six-base) MAPSORT of: pFN21ASDA0780.seq Check: 1834 from: 1 to: 7870 pFN21ASDA0780 7870 bp With 182 enzymes: SgfI * January 16, 2009 15:30 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3328 4840 3328 Size: 1512 6358 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5680 278 Size: 53 83 186 936 4144 2468 Fragments arranged by size: 4144 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3155 3494 5213 1937 Size: 1218 339 1719 4594 Fragments arranged by size: 4594 1719 1218 339 Acc65I G'GTAC_C Cuts at: 5191 5191 Size: 7870 AclI AA'CG_TT Cuts at: 1563 5997 6370 1563 Size: 4434 373 3063 Fragments arranged by size: 4434 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2062 3105 3291 4838 5933 7317 7585 1632 Size: 430 1043 186 1547 1095 1384 268 1917 Fragments arranged by size: 1917 1547 1384 1095 1043 430 268 186 AflII C'TTAA_G Cuts at: 820 1017 3388 4316 820 Size: 197 2371 928 4374 Fragments arranged by size: 4374 2371 928 197 AflIII A'CryG_T Cuts at: 1203 4914 6769 7746 1203 Size: 3711 1855 977 1327 Fragments arranged by size: 3711 1855 1327 977 AgeI A'CCGG_T Cuts at: 6701 6701 Size: 7870 AhdI GACnn_n'nnGTC Cuts at: 4616 6599 4616 Size: 1983 5887 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7870 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 4201 1184 Size: 3017 4853 AlwNI CAG_nnn'CTG Cuts at: 5613 7185 5613 Size: 1572 6298 ApaI G_GGCC'C Cuts at: 4345 4829 4345 Size: 484 7386 ApaLI G'TGCA_C Cuts at: 2944 5927 7083 2944 Size: 2983 1156 3731 Fragments arranged by size: 3731 2983 1156 ApoI r'AATT_y Cuts at: 1493 2825 2896 2996 3349 5056 5179 5405 5557 Size: 1332 71 100 353 1707 123 226 152 Cuts at: 5557 7666 1493 Size: 2109 1697 Fragments arranged by size: 2109 1707 1697 1332 353 226 152 123 100 71 AseI AT'TA_AT Cuts at: 160 1032 2436 2619 5118 6424 160 Size: 872 1404 183 2499 1306 1606 Fragments arranged by size: 2499 1606 1404 1306 872 183 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7870 AvaI C'yCGr_G Cuts at: 1870 1943 3512 5195 1870 Size: 73 1569 1683 4545 Fragments arranged by size: 4545 1683 1569 73 BamHI G'GATC_C Cuts at: 4940 5200 5567 4940 Size: 260 367 7243 Fragments arranged by size: 7243 367 260 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2413 3735 3793 5191 6646 Size: 325 217 627 626 1322 58 1398 1455 Cuts at: 6646 618 Size: 1842 Fragments arranged by size: 1842 1455 1398 1322 627 626 325 217 58 BanII G_rGCy'C Cuts at: 1391 2146 2643 4345 4829 5189 1391 Size: 755 497 1702 484 360 4072 Fragments arranged by size: 4072 1702 755 497 484 360 BbeI G_GCGC'C Cuts at: 3739 3797 3739 Size: 58 7812 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2579 2604 3414 928 Size: 971 680 25 810 5384 Fragments arranged by size: 5384 971 810 680 25 BbvCI CC'TCA_GC Cuts at: 3287 4836 3287 Size: 1549 6321 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 6061 6095 1903 Size: 34 4124 34 3678 Fragments arranged by size: 4124 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5762 6972 5762 Size: 1210 6660 BclI T'GATC_A Cuts at: 1548 2832 3052 1548 Size: 1284 220 6366 Fragments arranged by size: 6366 1284 220 BfrBI ATG'CAT Cuts at: 2735 3440 4452 2735 Size: 705 1012 6153 Fragments arranged by size: 6153 1012 705 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 6481 136 Size: 107 122 71 631 605 4809 1525 Fragments arranged by size: 4809 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3764 4230 7865 3764 Size: 466 3635 3769 Fragments arranged by size: 3769 3635 466 BlpI GC'TnA_GC Cuts at: 3912 5287 3912 Size: 1375 6495 Bme1580I G_kGCm'C Cuts at: 1790 2948 4255 4345 4546 4829 5931 7087 1790 Size: 1158 1307 90 201 283 1102 1156 2573 Fragments arranged by size: 2573 1307 1158 1156 1102 283 201 90 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7870 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3660 3690 5601 Size: 416 53 83 399 24 2243 30 1911 Cuts at: 5601 6559 442 Size: 958 1753 Fragments arranged by size: 2243 1911 1753 958 416 399 83 53 30 24 BmtI G_CTAG'C Cuts at: 1056 3330 1056 Size: 2274 5596 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3580 3580 Size: 7870 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2841 3054 3741 4506 6530 1239 Size: 1602 213 687 765 2024 2579 Fragments arranged by size: 2579 2024 1602 765 687 213 Bpu10I CC'TnA_GC Cuts at: 3287 4836 3287 Size: 1549 6321 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2682 3236 5995 6860 7158 2682 Size: 554 2759 865 298 3394 Fragments arranged by size: 3394 2759 865 554 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4486 6533 882 Size: 617 2987 2047 2219 Fragments arranged by size: 2987 2219 2047 617 BsaAI yAC'GTr Cuts at: 493 1204 5167 5661 493 Size: 711 3963 494 2702 Fragments arranged by size: 3963 2702 711 494 BsaBI GATnn'nnATC Cuts at: 7864 7864 Size: 7870 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3736 3794 5677 6059 Size: 53 83 186 936 2203 58 1883 382 Cuts at: 6059 6694 275 Size: 635 1451 Fragments arranged by size: 2203 1883 1451 936 635 382 186 83 58 53 BsaWI w'CCGG_w Cuts at: 2665 6303 6701 6975 7122 7533 2665 Size: 3638 398 274 147 411 3002 Fragments arranged by size: 3638 3002 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2372 2402 4833 4863 1178 Size: 30 165 30 969 30 2431 30 4185 Fragments arranged by size: 4185 2431 969 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2108 3121 3124 3848 4320 1186 Size: 922 1013 3 724 472 4736 Fragments arranged by size: 4736 1013 922 724 472 3 BseYI C'CCAG_C Cuts at: 2057 3655 4553 4822 5088 7073 7569 2057 Size: 1598 898 269 266 1985 496 2358 Fragments arranged by size: 2358 1985 1598 898 496 269 266 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2448 2904 3949 1163 Size: 573 712 456 1045 5084 Fragments arranged by size: 5084 1045 712 573 456 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4513 5320 6081 7109 1540 Size: 275 186 2512 807 761 1028 2301 Fragments arranged by size: 2512 2301 1028 807 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2948 4315 5931 6016 7087 2948 Size: 1367 1616 85 1071 3731 Fragments arranged by size: 3731 1616 1367 1071 85 BsiWI C'GTAC_G Cuts at: 4189 4189 Size: 7870 BsmI GAATG_Cn' Cuts at: 4289 5381 5474 4289 Size: 1092 93 6685 Fragments arranged by size: 6685 1092 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2146 2643 2948 4255 4315 4345 4546 Size: 399 356 497 305 1307 60 30 201 Cuts at: 4546 4829 5189 5931 6016 7087 1391 Size: 283 360 742 85 1071 2174 Fragments arranged by size: 2174 1307 1071 742 497 399 360 356 305 283 201 85 60 30 BspEI T'CCGG_A Cuts at: 2665 7533 2665 Size: 4868 3002 BspHI T'CATG_A Cuts at: 2829 4225 5759 7489 2829 Size: 1396 1534 1730 3210 Fragments arranged by size: 3210 1730 1534 1396 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3328 4840 5054 5225 844 Size: 2484 1512 214 171 3489 Fragments arranged by size: 3489 2484 1512 214 171 BsrBI CCG'CTC Cuts at: 1394 1823 5757 1394 Size: 429 3934 3507 Fragments arranged by size: 3934 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4009 4959 6365 6539 65 Size: 1183 2761 950 1406 174 1396 Fragments arranged by size: 2761 1406 1396 1183 950 174 BsrFI r'CCGG_y Cuts at: 1700 1810 6514 6701 1700 Size: 110 4704 187 2869 Fragments arranged by size: 4704 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7870 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7870 BssSI C'ACGA_G Cuts at: 5930 6942 5930 Size: 1012 6858 BstAPI GCAn_nnn'nTGC Cuts at: 794 4458 794 Size: 3664 4206 BstBI TT'CG_AA Cuts at: 2007 6674 2007 Size: 4667 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7870 BstXI CCAn_nnnn'nTGG Cuts at: 2029 2819 3632 4553 2029 Size: 790 813 921 5346 Fragments arranged by size: 5346 921 813 790 BstYI r'GATC_y Cuts at: 1972 3764 4230 4940 5200 5567 5952 5969 7421 Size: 1792 466 710 260 367 385 17 1452 Cuts at: 7421 7507 7519 7865 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1792 1452 710 466 385 367 346 260 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2178 3171 3816 4791 2178 Size: 993 645 975 5257 Fragments arranged by size: 5257 993 975 645 BtgI C'CryG_G Cuts at: 513 1065 2022 2088 3625 4662 513 Size: 552 957 66 1537 1037 3721 Fragments arranged by size: 3721 1537 1037 957 552 66 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2315 4716 487 Size: 683 56 1089 2401 3641 Fragments arranged by size: 3641 2401 1089 683 56 BtsI GCAGTG_nn' Cuts at: 3285 5386 6180 6200 3285 Size: 2101 794 20 4955 Fragments arranged by size: 4955 2101 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7870 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 4841 4876 529 Size: 35 4277 35 3523 Fragments arranged by size: 4277 3523 35 35 DraI TTT'AAA Cuts at: 5174 5174 Size: 7870 DraIII CAC_nnn'GTG Cuts at: 3956 3956 Size: 7870 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3254 6877 809 Size: 507 1938 3623 1802 Fragments arranged by size: 3623 1938 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2031 2328 3542 Size: 54 1425 50 275 18 201 297 1214 Cuts at: 3542 3936 5317 6206 7835 8 Size: 394 1381 889 1629 43 Fragments arranged by size: 1629 1425 1381 1214 889 394 297 275 201 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5317 1537 Size: 275 3505 4090 Fragments arranged by size: 4090 3505 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2508 3180 3309 3600 4140 4952 4980 5800 Size: 1155 672 129 291 540 812 28 820 Cuts at: 5800 1353 Size: 3423 Fragments arranged by size: 3423 1155 820 812 672 540 291 129 28 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 6457 6831 6977 387 Size: 1064 138 4868 374 146 1280 Fragments arranged by size: 4868 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2062 2841 3054 3105 3291 3741 4506 Size: 393 430 779 213 51 186 450 765 Cuts at: 4506 4838 5933 6530 7317 7585 1239 Size: 332 1095 597 787 268 1524 Fragments arranged by size: 1524 1095 787 779 765 597 450 430 393 332 268 213 186 51 EcoNI CCTnn'n_nnAGG Cuts at: 3163 4145 3163 Size: 982 6888 EcoO109I rG'GnC_Cy Cuts at: 3220 5043 3220 Size: 1823 6047 EcoRI G'AATT_C Cuts at: 2825 5056 5179 5557 2825 Size: 2231 123 378 5138 Fragments arranged by size: 5138 2231 378 123 EcoRV GAT'ATC Cuts at: 2983 3014 4798 2983 Size: 31 1784 6055 Fragments arranged by size: 6055 1784 31 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3471 3471 Size: 7870 FspI TGC'GCA Cuts at: 6376 6376 Size: 7870 HaeII r_GCGC'y Cuts at: 1678 3739 3797 7017 1678 Size: 2061 58 3220 2531 Fragments arranged by size: 3220 2531 2061 58 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2630 2662 3179 3211 3809 Size: 32 135 32 1058 32 517 32 598 Cuts at: 3809 3841 4479 4511 4602 4634 4751 4783 4833 Size: 32 638 32 91 32 117 32 50 Cuts at: 4833 4865 4953 4985 6511 6543 6585 6617 1373 Size: 32 88 32 1526 32 42 32 2626 Fragments arranged by size: 2626 1526 1058 638 598 517 135 117 91 88 50 42 32 32 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3594 4621 5214 5460 669 Size: 988 281 1656 1027 593 246 3079 Fragments arranged by size: 3079 1656 1027 988 593 281 246 HindIII A'AGCT_T Cuts at: 748 3136 3432 748 Size: 2388 296 5186 Fragments arranged by size: 5186 2388 296 HpaI GTT'AAC Cuts at: 3594 5460 3594 Size: 1866 6004 KasI G'GCGC_C Cuts at: 3735 3793 3735 Size: 58 7812 KpnI G_GTAC'C Cuts at: 5195 5195 Size: 7870 MfeI C'AATT_G Cuts at: 5469 5469 Size: 7870 MluI A'CGCG_T Cuts at: 7746 7746 Size: 7870 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 6984 7168 1305 Size: 5679 184 2007 Fragments arranged by size: 5679 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2330 3544 3938 10 Size: 54 1425 343 498 1214 394 3942 Fragments arranged by size: 3942 1425 1214 498 394 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2027 3630 4661 5828 6187 6346 518 Size: 648 861 1603 1031 1167 359 159 2042 Fragments arranged by size: 2042 1603 1167 1031 861 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2061 2326 3384 3659 3911 5286 5963 7111 Size: 455 265 1058 275 252 1375 677 1148 Cuts at: 7111 7356 1606 Size: 245 2120 Fragments arranged by size: 2120 1375 1148 1058 677 455 275 265 252 245 NarI GG'CG_CC Cuts at: 3736 3794 3736 Size: 58 7812 NcoI C'CATG_G Cuts at: 513 1065 2022 2088 3625 513 Size: 552 957 66 1537 4758 Fragments arranged by size: 4758 1537 957 552 66 NdeI CA'TA_TG Cuts at: 387 2845 387 Size: 2458 5412 NheI G'CTAG_C Cuts at: 1052 3326 1052 Size: 2274 5596 NotI GC'GGCC_GC Cuts at: 5317 5317 Size: 7870 NsiI A_TGCA'T Cuts at: 2737 3442 4454 2737 Size: 705 1012 6153 Fragments arranged by size: 6153 1012 705 NspI r_CATG'y Cuts at: 1132 1229 2569 4605 4918 5228 6773 1132 Size: 97 1340 2036 313 310 1545 2229 Fragments arranged by size: 2229 2036 1545 1340 313 310 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7870 PciI A'CATG_T Cuts at: 4914 6769 4914 Size: 1855 6015 PflMI CCAn_nnn'nTGG Cuts at: 1108 2649 1108 Size: 1541 6329 PfoI T'CCnGG_A Cuts at: 2100 2100 Size: 7870 PmeI GTTT'AAAC Cuts at: 5174 5174 Size: 7870 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3707 3739 5939 5971 7452 7484 3707 Size: 32 2200 32 1481 32 4093 Fragments arranged by size: 4093 2200 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 3220 5043 3220 Size: 1823 6047 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7870 PsiI TTA'TAA Cuts at: 5440 5440 Size: 7870 PspOMI G'GGCC_C Cuts at: 4341 4825 4341 Size: 484 7386 PstI C_TGCA'G Cuts at: 830 3105 4463 5222 830 Size: 2275 1358 759 3478 Fragments arranged by size: 3478 2275 1358 759 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7870 PvuII CAG'CTG Cuts at: 2061 2326 3384 3911 2061 Size: 265 1058 527 6020 Fragments arranged by size: 6020 1058 527 265 SalI G'TCGA_C Cuts at: 1936 5212 1936 Size: 3276 4594 SbfI CC_TGCA'GG Cuts at: 5222 5222 Size: 7870 ScaI AGT'ACT Cuts at: 2390 6118 7830 2390 Size: 3728 1712 2430 Fragments arranged by size: 3728 2430 1712 SexAI A'CCwGG_T Cuts at: 4920 4920 Size: 7870 SfcI C'TryA_G Cuts at: 826 1045 2611 3101 4459 5218 6353 7034 7225 Size: 219 1566 490 1358 759 1135 681 191 Cuts at: 7225 826 Size: 1471 Fragments arranged by size: 1566 1471 1358 1135 759 681 490 219 191 SfoI GGC'GCC Cuts at: 3737 3795 3737 Size: 58 7812 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7870 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7870 SmaI CCC'GGG Cuts at: 1872 5197 1872 Size: 3325 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2697 3251 3388 4316 5974 6875 Size: 197 926 754 554 137 928 1658 901 Cuts at: 6875 7137 820 Size: 262 1553 Fragments arranged by size: 1658 1553 928 926 901 754 554 262 197 137 SnaBI TAC'GTA Cuts at: 493 5167 5661 493 Size: 4674 494 2702 Fragments arranged by size: 4674 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 7870 SphI G_CATG'C Cuts at: 1132 5228 1132 Size: 4096 3774 SspI AAT'ATT Cuts at: 5 52 2345 4121 4892 4906 5794 5 Size: 47 2293 1776 771 14 888 2081 Fragments arranged by size: 2293 2081 1776 888 771 47 14 StuI AGG'CCT Cuts at: 1517 3169 3754 1517 Size: 1652 585 5633 Fragments arranged by size: 5633 1652 585 StyI C'CwwG_G Cuts at: 513 1065 2022 2088 2722 3625 4638 5309 513 Size: 552 957 66 634 903 1013 671 3074 Fragments arranged by size: 3074 1013 957 903 671 634 552 66 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4144 5897 5914 6067 6252 6697 325 Size: 1255 2564 1753 17 153 185 445 1498 Fragments arranged by size: 2564 1753 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2280 2348 2388 Size: 274 80 33 51 1443 303 68 40 Cuts at: 2388 3392 6116 7828 96 Size: 1004 2724 1712 138 Fragments arranged by size: 2724 1712 1443 1004 303 274 138 80 68 51 40 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 7830 7862 7830 Size: 32 7838 Tth111I GACn'n_nGTC Cuts at: 1316 3236 3892 1316 Size: 1920 656 5294 Fragments arranged by size: 5294 1920 656 XbaI T'CTAG_A Cuts at: 5206 5206 Size: 7870 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7870 XmaI C'CCGG_G Cuts at: 1870 5195 1870 Size: 3325 4545 XmnI GAAnn'nnTTC Cuts at: 2315 5999 2315 Size: 3684 4186 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5678 276 Size: 53 83 186 936 4144 2468 Fragments arranged by size: 4144 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BsmBI BstZ17I EcoICRI FseI FspAI NaeI NgoMIV NruI PacI PmlI PspXI PsrI RsrII SacI SacII SanDI SapI SfiI SrfI SwaI XcmI