(Circular) (Six-base) MAPSORT of: pFN21ASDA0755.seq Check: 7395 from: 1 to: 7825 pFN21ASDA0755 7825 bp With 182 enzymes: SgfI * January 16, 2009 15:30 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3464 5635 278 Size: 53 83 186 936 1928 2171 2468 Fragments arranged by size: 2468 2171 1928 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4795 5168 1937 Size: 2858 373 4594 Fragments arranged by size: 4594 2858 373 Acc65I G'GTAC_C Cuts at: 5146 5146 Size: 7825 AclI AA'CG_TT Cuts at: 1563 5952 6325 1563 Size: 4389 373 3063 Fragments arranged by size: 4389 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3437 3804 4923 5888 7272 7540 1632 Size: 1805 367 1119 965 1384 268 1917 Fragments arranged by size: 1917 1805 1384 1119 965 367 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7628 AflIII A'CryG_T Cuts at: 1203 3129 3657 4701 4800 6724 7701 1203 Size: 1926 528 1044 99 1924 977 1327 Fragments arranged by size: 1926 1924 1327 1044 977 528 99 AgeI A'CCGG_T Cuts at: 6656 6656 Size: 7825 AhdI GACnn_n'nnGTC Cuts at: 4637 6554 4637 Size: 1917 5908 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7825 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7825 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2514 2546 2514 Size: 32 7793 AlwNI CAG_nnn'CTG Cuts at: 5032 5568 7140 5032 Size: 536 1572 5717 Fragments arranged by size: 5717 1572 536 ApaLI G'TGCA_C Cuts at: 5882 7038 5882 Size: 1156 6669 ApoI r'AATT_y Cuts at: 1493 3435 4443 5134 5360 5512 7621 1493 Size: 1942 1008 691 226 152 2109 1697 Fragments arranged by size: 2109 1942 1697 1008 691 226 152 AseI AT'TA_AT Cuts at: 160 1032 6379 160 Size: 872 5347 1606 Fragments arranged by size: 5347 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7825 AvaI C'yCGr_G Cuts at: 1870 1943 5150 1870 Size: 73 3207 4545 Fragments arranged by size: 4545 3207 73 BamHI G'GATC_C Cuts at: 2113 2800 5155 5522 2113 Size: 687 2355 367 4416 Fragments arranged by size: 4416 2355 687 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2567 5146 6601 618 Size: 325 217 627 780 2579 1455 1842 Fragments arranged by size: 2579 1842 1455 780 627 325 217 BanII G_rGCy'C Cuts at: 1391 2245 2610 5144 1391 Size: 854 365 2534 4072 Fragments arranged by size: 4072 2534 854 365 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3250 3425 4245 4303 5046 928 Size: 971 1351 175 820 58 743 3707 Fragments arranged by size: 3707 1351 971 820 743 175 58 BbvCI CC'TCA_GC Cuts at: 2067 3975 2067 Size: 1908 5917 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 6016 6050 1903 Size: 34 4079 34 3678 Fragments arranged by size: 4079 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5717 6927 5717 Size: 1210 6615 BclI T'GATC_A Cuts at: 1548 1548 Size: 7825 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2158 6436 136 Size: 107 122 71 631 605 486 4278 1525 Fragments arranged by size: 4278 1525 631 605 486 122 107 71 BglII A'GATC_T Cuts at: 4602 5100 7820 4602 Size: 498 2720 4607 Fragments arranged by size: 4607 2720 498 BlpI GC'TnA_GC Cuts at: 3000 5242 3000 Size: 2242 5583 Bme1580I G_kGCm'C Cuts at: 1790 2106 3137 5886 7042 1790 Size: 316 1031 2749 1156 2573 Fragments arranged by size: 2749 2573 1156 1031 316 BmgBI CAC'GTC Cuts at: 1318 2988 3952 1318 Size: 1670 964 5191 Fragments arranged by size: 5191 1670 964 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2629 5556 6514 Size: 416 53 83 399 24 1212 2927 958 Cuts at: 6514 442 Size: 1753 Fragments arranged by size: 2927 1753 1212 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 4343 1056 Size: 3287 4538 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3345 3345 Size: 7825 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2772 4005 6485 1239 Size: 1533 1233 2480 2579 Fragments arranged by size: 2579 2480 1533 1233 Bpu10I CC'TnA_GC Cuts at: 2067 3975 2067 Size: 1908 5917 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2511 5950 6815 7113 2511 Size: 3439 865 298 3223 Fragments arranged by size: 3439 3223 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 6488 882 Size: 617 4989 2219 Fragments arranged by size: 4989 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2041 5122 5616 493 Size: 711 837 3081 494 2702 Fragments arranged by size: 3081 2702 837 711 494 BsaBI GATnn'nnATC Cuts at: 7819 7819 Size: 7825 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3461 5632 6014 6649 Size: 53 83 186 936 1928 2171 382 635 Cuts at: 6649 275 Size: 1451 Fragments arranged by size: 2171 1928 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4482 6258 6656 6930 7077 7488 4482 Size: 1776 398 274 147 411 4819 Fragments arranged by size: 4819 1776 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2116 2146 2850 2880 1178 Size: 30 165 30 713 30 704 30 6123 Fragments arranged by size: 6123 713 704 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2424 2475 2610 2613 2871 1186 Size: 1238 51 135 3 258 6140 Fragments arranged by size: 6140 1238 258 135 51 3 BseYI C'CCAG_C Cuts at: 2376 2380 3534 3961 7028 7524 2376 Size: 4 1154 427 3067 496 2677 Fragments arranged by size: 3067 2677 1154 496 427 4 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2316 2745 1163 Size: 573 580 429 6243 Fragments arranged by size: 6243 580 573 429 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5275 6036 7064 1540 Size: 275 186 3274 761 1028 2301 Fragments arranged by size: 3274 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2245 5886 5971 7042 2245 Size: 3641 85 1071 3028 Fragments arranged by size: 3641 3028 1071 85 BsmI GAATG_Cn' Cuts at: 5336 5429 5336 Size: 93 7732 BsmBI CGTCTCn'nnnn_ Cuts at: 3452 3452 Size: 7825 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2106 2245 2610 3137 5144 5886 5971 Size: 399 316 139 365 527 2007 742 85 Cuts at: 5971 7042 1391 Size: 1071 2174 Fragments arranged by size: 2174 2007 1071 742 527 399 365 316 139 85 BspEI T'CCGG_A Cuts at: 7488 7488 Size: 7825 BspHI T'CATG_A Cuts at: 3354 4641 5714 7444 3354 Size: 1287 1073 1730 3735 Fragments arranged by size: 3735 1730 1287 1073 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4738 5180 844 Size: 3894 442 3489 Fragments arranged by size: 3894 3489 442 BsrBI CCG'CTC Cuts at: 1394 1823 5712 1394 Size: 429 3889 3507 Fragments arranged by size: 3889 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2474 4892 6320 6494 65 Size: 1183 1226 2418 1428 174 1396 Fragments arranged by size: 2418 1428 1396 1226 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 6469 6656 1700 Size: 110 4659 187 2869 Fragments arranged by size: 4659 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2592 2963 4554 96 Size: 2496 371 1591 3367 Fragments arranged by size: 3367 2496 1591 371 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7825 BssSI C'ACGA_G Cuts at: 5885 6897 5885 Size: 1012 6813 BstAPI GCAn_nnn'nTGC Cuts at: 794 3117 794 Size: 2323 5502 BstBI TT'CG_AA Cuts at: 2007 6629 2007 Size: 4622 3203 BstEII G'GTnAC_C Cuts at: 1184 2255 1184 Size: 1071 6754 BstXI CCAn_nnnn'nTGG Cuts at: 2228 4654 2228 Size: 2426 5399 BstYI r'GATC_y Cuts at: 1972 2113 2522 2800 2892 3281 4602 5100 5155 Size: 141 409 278 92 389 1321 498 55 Cuts at: 5155 5522 5907 5924 7376 7462 7474 7820 1972 Size: 367 385 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1321 498 409 389 385 367 346 278 141 92 86 55 17 12 BtgI C'CryG_G Cuts at: 513 1065 4206 4647 513 Size: 552 3141 441 3691 Fragments arranged by size: 3691 3141 552 441 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 7086 Fragments arranged by size: 7086 683 56 BtsI GCAGTG_nn' Cuts at: 4206 5341 6135 6155 4206 Size: 1135 794 20 5876 Fragments arranged by size: 5876 1135 794 20 ClaI AT'CG_AT Cuts at: 1554 4185 1554 Size: 2631 5194 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3456 3491 529 Size: 35 2892 35 4863 Fragments arranged by size: 4863 2892 35 35 DraI TTT'AAA Cuts at: 4405 5129 4405 Size: 724 7101 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6832 809 Size: 507 5516 1802 Fragments arranged by size: 5516 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3885 5272 6161 Size: 54 1425 50 275 18 2055 1387 889 Cuts at: 6161 7790 8 Size: 1629 43 Fragments arranged by size: 2055 1629 1425 1387 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5272 1537 Size: 275 3460 4090 Fragments arranged by size: 4090 3460 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2658 3441 3450 3515 3929 5068 5755 1353 Size: 1305 783 9 65 414 1139 687 3423 Fragments arranged by size: 3423 1305 1139 783 687 414 65 9 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2766 6412 6786 6932 387 Size: 1064 138 1177 3646 374 146 1280 Fragments arranged by size: 3646 1280 1177 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2243 2243 Size: 7825 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2772 3437 3804 4005 4923 5888 6485 Size: 393 1140 665 367 201 918 965 597 Cuts at: 6485 7272 7540 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1140 965 918 787 665 597 393 367 268 201 EcoNI CCTnn'n_nnAGG Cuts at: 2076 2655 2076 Size: 579 7246 EcoO109I rG'GnC_Cy Cuts at: 2159 2201 2279 2351 2447 2756 2159 Size: 42 78 72 96 309 7228 Fragments arranged by size: 7228 309 96 78 72 42 EcoRI G'AATT_C Cuts at: 5134 5512 5134 Size: 378 7447 EcoRV GAT'ATC Cuts at: 2681 3170 2681 Size: 489 7336 FspI TGC'GCA Cuts at: 6331 6331 Size: 7825 HaeII r_GCGC'y Cuts at: 1678 6972 1678 Size: 5294 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2594 2626 2758 2790 2792 Size: 32 135 32 1022 32 132 32 2 Cuts at: 2792 2824 2877 2909 3941 3973 4239 4271 4297 Size: 32 53 32 1032 32 266 32 26 Cuts at: 4297 4329 4357 4389 4587 4619 4623 4655 6466 Size: 32 28 32 198 32 4 32 1811 Cuts at: 6466 6498 6540 6572 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1811 1032 1022 266 198 135 132 53 42 32 32 32 32 32 32 32 32 32 32 32 32 32 32 28 26 4 2 HincII GTy'rAC Cuts at: 669 1657 1938 2030 2293 3604 5169 5415 669 Size: 988 281 92 263 1311 1565 246 3079 Fragments arranged by size: 3079 1565 1311 988 281 263 246 92 HindIII A'AGCT_T Cuts at: 748 748 Size: 7825 HpaI GTT'AAC Cuts at: 5415 5415 Size: 7825 KpnI G_GTAC'C Cuts at: 5150 5150 Size: 7825 MfeI C'AATT_G Cuts at: 4986 5424 4986 Size: 438 7387 MluI A'CGCG_T Cuts at: 7701 7701 Size: 7825 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2159 3244 3544 3567 3621 4978 5047 6939 Size: 854 1085 300 23 54 1357 69 1892 Cuts at: 6939 7123 1305 Size: 184 2007 Fragments arranged by size: 2007 1892 1357 1085 854 300 184 69 54 23 MscI TGG'CCA Cuts at: 10 64 1489 1832 3887 10 Size: 54 1425 343 2055 3948 Fragments arranged by size: 3948 2055 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4646 4652 4886 5783 6142 6301 518 Size: 648 3480 6 234 897 359 159 2042 Fragments arranged by size: 3480 2042 897 648 359 234 159 6 MspA1I CmG'CkG Cuts at: 1606 2380 4636 5110 5241 5918 7066 7311 1606 Size: 774 2256 474 131 677 1148 245 2120 Fragments arranged by size: 2256 2120 1148 774 677 474 245 131 NcoI C'CATG_G Cuts at: 513 1065 4647 513 Size: 552 3582 3691 Fragments arranged by size: 3691 3582 552 NdeI CA'TA_TG Cuts at: 387 3404 4467 387 Size: 3017 1063 3745 Fragments arranged by size: 3745 3017 1063 NheI G'CTAG_C Cuts at: 1052 4339 1052 Size: 3287 4538 NotI GC'GGCC_GC Cuts at: 5272 5272 Size: 7825 NspI r_CATG'y Cuts at: 1132 1229 2315 3133 3661 4479 4804 5183 6728 Size: 97 1086 818 528 818 325 379 1545 Cuts at: 6728 1132 Size: 2229 Fragments arranged by size: 2229 1545 1086 818 818 528 379 325 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7825 PciI A'CATG_T Cuts at: 3129 3657 4800 6724 3129 Size: 528 1143 1924 4230 Fragments arranged by size: 4230 1924 1143 528 PflMI CCAn_nnn'nTGG Cuts at: 1108 2213 2668 3226 4471 1108 Size: 1105 455 558 1245 4462 Fragments arranged by size: 4462 1245 1105 558 455 PfoI T'CCnGG_A Cuts at: 2212 4437 2212 Size: 2225 5600 PmeI GTTT'AAAC Cuts at: 5129 5129 Size: 7825 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5894 5926 7407 7439 5894 Size: 32 1481 32 6280 Fragments arranged by size: 6280 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2201 2351 2447 2756 2201 Size: 150 96 309 7270 Fragments arranged by size: 7270 309 150 96 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7825 PsiI TTA'TAA Cuts at: 4353 5395 4353 Size: 1042 6783 PstI C_TGCA'G Cuts at: 830 4331 4510 5177 830 Size: 3501 179 667 3478 Fragments arranged by size: 3501 3478 667 179 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7825 PvuII CAG'CTG Cuts at: 2380 4636 5110 2380 Size: 2256 474 5095 Fragments arranged by size: 5095 2256 474 SacI G_AGCT'C Cuts at: 2245 2245 Size: 7825 SalI G'TCGA_C Cuts at: 1936 5167 1936 Size: 3231 4594 SanDI GG'GwC_CC Cuts at: 2201 2447 2201 Size: 246 7579 SapI GCTCTTCn'nnn_ Cuts at: 3441 5068 3441 Size: 1627 6198 SbfI CC_TGCA'GG Cuts at: 5177 5177 Size: 7825 ScaI AGT'ACT Cuts at: 6073 7785 6073 Size: 1712 6113 SfcI C'TryA_G Cuts at: 826 1045 2910 4327 4506 5173 6308 6989 7180 Size: 219 1865 1417 179 667 1135 681 191 Cuts at: 7180 826 Size: 1471 Fragments arranged by size: 1865 1471 1417 1135 681 667 219 191 179 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7825 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7825 SmaI CCC'GGG Cuts at: 1872 5152 1872 Size: 3280 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2526 5929 6830 7092 820 Size: 197 926 583 3403 901 262 1553 Fragments arranged by size: 3403 1553 926 901 583 262 197 SnaBI TAC'GTA Cuts at: 493 5122 5616 493 Size: 4629 494 2702 Fragments arranged by size: 4629 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 7825 SphI G_CATG'C Cuts at: 1132 5183 1132 Size: 4051 3774 SspI AAT'ATT Cuts at: 5 52 4776 4851 5749 5 Size: 47 4724 75 898 2081 Fragments arranged by size: 4724 2081 898 75 47 StuI AGG'CCT Cuts at: 1517 2082 3125 1517 Size: 565 1043 6217 Fragments arranged by size: 6217 1043 565 StyI C'CwwG_G Cuts at: 513 1065 2667 2932 3888 4647 4843 5264 513 Size: 552 1602 265 956 759 196 421 3074 Fragments arranged by size: 3074 1602 956 759 552 421 265 196 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2390 2560 2660 4640 5852 5869 6022 Size: 1255 810 170 100 1980 1212 17 153 Cuts at: 6022 6207 6652 325 Size: 185 445 1498 Fragments arranged by size: 1980 1498 1255 1212 810 445 185 170 153 100 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2592 2963 4554 Size: 274 80 33 51 1443 615 371 1591 Cuts at: 4554 4582 4718 6071 7783 96 Size: 28 136 1353 1712 138 Fragments arranged by size: 1712 1591 1443 1353 615 371 274 138 136 80 51 33 28 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2116 2148 4477 4509 7785 7817 2116 Size: 32 2329 32 3276 32 2124 Fragments arranged by size: 3276 2329 2124 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7825 XbaI T'CTAG_A Cuts at: 5161 5161 Size: 7825 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2450 3895 2450 Size: 1445 6380 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7825 XmaI C'CCGG_G Cuts at: 1870 5150 1870 Size: 3280 4545 XmnI GAAnn'nnTTC Cuts at: 3435 3752 5954 3435 Size: 317 2202 5306 Fragments arranged by size: 5306 2202 317 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3462 5633 276 Size: 53 83 186 936 1928 2171 2468 Fragments arranged by size: 2468 2171 1928 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SanDI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BaeI BbeI BfrBI BsiWI BstZ17I Bsu36I DraIII FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacII SexAI SfiI SfoI SrfI SwaI