Restriction map for
pFN21ASDA0745
##################################
# NO CUT ENZYMES ( 97 enzymes) #
##################################
AccI AccIII AclI AflII AgeI
AhdI AocI Aor51HI ApaI AscI
AspEI AvaI AviII AvrII BamHI
BbeI BbrPI BbvCI BcgI BciVI
BclI BfrI BglI BlnI BseAI
BsiWI BsoBI BspDI BspEI BspLU11I
BspMI BsrBI BsrFI BssHII Bst1107I
BstBI BstEII BstPI BstXI BstZ17I
Bsu36I Cfr10I ClaI CpoI DraI
DrdI Eam1105I Eco47III Eco81I EcoO65I
EcoT22I FbaI FseI FspI HpaI
KasI KspI MluI MroI NaeI
NarI NgoMIV NheI NotI NsiI
NspV PacI PaeR7I PinAI PmaCI
PmeI PmlI Psp1406I PvuI RsrII
SacII SalI SapI SbfI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspI
StuI SwaI XbaI XcmI XhoI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 56 enzymes) #
###################################
AatII 406 GACGTC GACGT^C
Acc65I 1736 GGTACC G^GTACC
AccII 544 CGCG CG^CG
AflIII 823 ACGTGT A^C[GA][CT]GT
Alw44I 827 GTGCAC G^TGCAC
ApaLI 827 GTGCAC G^TGCAC
AseI 484 ATTAAT AT^TAAT
AsnI 484 ATTAAT AT^TAAT
Asp718I 1736 GGTACC G^GTACC
AspI 379 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 1097 TGGCCA TGG^CCA
BanI 1736 GGTACC G^G[CT][GA]CC
BanII 1729 GAGCTC G[GA]GC[CT]^C
BcnI 972 CCGGG CC^[GC]GG
BglII 1130 AGATCT A^GATCT
BsaAI 2306 CACGTA [CT]AC^GT[GA]
BsaBI 1501 GATGAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 2538 ACCGGA [AT]^CCGG[AT]
BsmI 2644 GCATTC GAATGC(1/-1)
Bsp1407I 730 TGTACA T^GTACA
BspHI 1670 TCATGA T^CATGA
BsrGI 730 TGTACA T^GTACA
BstUI 544 CGCG CG^CG
BstYI 1130 AGATCT [GA]^GATC[CT]
DraIII 2275 CACATTGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 289 CGGCCG C^GGCCG
EclXI 289 CGGCCG C^GGCCG
Eco52I 289 CGGCCG C^GGCCG
EcoRI 2676 GAATTC G^AATTC
EcoRV 562 GATATC GAT^ATC
FauI 1075 GCGGG CCCGC(4/6)
HaeII 886 AGCGCC [GA]GCGC^[CT]
HincII 2659 GTCAAC GT[CT]^[GA]AC
HindII 2659 GTCAAC GT[CT]^[GA]AC
KpnI 1736 GGTACC GGTAC^C
MamI 1501 GATGAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 1724 CAATTG C^AATTG
MflI 1130 AGATCT [GA]^GATC[CT]
MluNI 1097 TGGCCA TGG^CCA
MscI 1097 TGGCCA TGG^CCA
MslI 909 CATTGCAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MunI 1724 CAATTG C^AATTG
MvnI 544 CGCG CG^CG
NciI 972 CCGGG CC^[GC]GG
NdeI 2155 CATATG CA^TATG
NruI 543 TCGCGA TCG^CGA
PflFI 379 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 1209 GACCATGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 484 ATTAAT AT^TAAT
PstI 2047 CTGCAG CTGCA^G
RcaI 1670 TCATGA T^CATGA
SacI 1729 GAGCTC GAGCT^C
ScaI 1256 AGTACT AGT^ACT
SspBI 730 TGTACA T^GTACA
Tth111I 379 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoII 1130 AGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AcsI 106 GAATTT [GA]^AATT[CT]
2676 GAATTC
ApoI 106 GAATTT [GA]^AATT[CT]
2676 GAATTC
Asp700I 1117 GAACAGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1783 GAATTGGTTC
BlpI 1104 GCTGAGC GC^T[ACGT]AGC
2109 GCTCAGC
Bpu1102I 1104 GCTGAGC GC^T[ACGT]AGC
2109 GCTCAGC
BsaI 1207 GAGACC GGTCTC(1/5)
1625 GAGACC
BsiEI 289 CGGCCG CG[GA][CT]^CG
2349 CGACCG
BsrDI 909 CATTGC GCAATG(2/0)
2699 GCAATG
BssSI 1383 CACGAG CACGAG(-5/-1)
835 CTCGTG
CelII 1104 GCTGAGC GC^T[ACGT]AGC
2109 GCTCAGC
CfoI 887 GCGC GCG^C
893 GCGC
DraII 1798 AGGTCCC [GA]G^G[ACGT]CC[CT]
1907 AGGTCCC
EcoNI 2142 CCTGACCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2334 CCTGAACCAGG
EcoO109I 1798 AGGTCCC [GA]G^G[ACGT]CC[CT]
1907 AGGTCCC
HapII 972 CCGG C^CGG
2539 CCGG
HhaI 887 GCGC GCG^C
893 GCGC
HinP1I 887 GCGC G^CGC
893 GCGC
HindIII 205 AAGCTT A^AGCTT
1348 AAGCTT
HpaII 972 CCGG C^CGG
2539 CCGG
MspI 972 CCGG C^CGG
2539 CCGG
NspI 193 ACATGC [GA]CATG^[CT]
1436 ACATGC
PpuMI 1798 AGGTCCC [GA]G^G[AT]CC[CT]
1907 AGGTCCC
XmnI 1117 GAACAGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1783 GAATTGGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 82 enzymes) #
#########################################
AciI AcyI AfaI AluI AlwI
AlwNI AspHI AvaII BbsI BbvI
BfaI BmyI BpmI BpuAI BsaHI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmBI BsmFI Bsp1286I
BsrI BssKI BstAPI BstF5I BstNI
Cac8I Cfr13I DdeI DpnII DsaI
EaeI EarI EcoRII EcoT14I Fnu4HI
FokI HaeIII HgaI Hin1I HinfI
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MnlI MseI
MspA1I MvaI MwoI NcoI NdeII
NlaIII NlaIV PflMI PleI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I