(Circular) (Six-base) MAPSORT of: pFN21ASDA0745.seq Check: 3388 from: 1 to: 7444 pFN21ASDA0745 7444 bp With 182 enzymes: SgfI * January 16, 2009 15:30 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2433 5254 278 Size: 53 83 186 936 897 2821 2468 Fragments arranged by size: 2821 2468 936 897 186 83 53 AccI GT'mk_AC Cuts at: 1937 4787 1937 Size: 2850 4594 Acc65I G'GTAC_C Cuts at: 3759 4765 3759 Size: 1006 6438 AclI AA'CG_TT Cuts at: 1563 5571 5944 1563 Size: 4008 373 3063 Fragments arranged by size: 4008 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2395 2607 3977 4013 4260 5507 6891 7159 Size: 763 212 1370 36 247 1247 1384 268 Cuts at: 7159 1632 Size: 1917 Fragments arranged by size: 1917 1384 1370 1247 763 268 247 212 36 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7247 AflIII A'CryG_T Cuts at: 1203 2846 6343 7320 1203 Size: 1643 3497 977 1327 Fragments arranged by size: 3497 1643 1327 977 AgeI A'CCGG_T Cuts at: 6275 6275 Size: 7444 AhdI GACnn_n'nnGTC Cuts at: 6173 6173 Size: 7444 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7444 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2233 1184 Size: 1049 6395 AlwNI CAG_nnn'CTG Cuts at: 2044 2206 2899 4423 4660 5187 6759 2044 Size: 162 693 1524 237 527 1572 2729 Fragments arranged by size: 2729 1572 1524 693 527 237 162 ApaLI G'TGCA_C Cuts at: 2850 5501 6657 2850 Size: 2651 1156 3637 Fragments arranged by size: 3637 2651 1156 ApoI r'AATT_y Cuts at: 1493 2129 4699 4753 4979 5131 7240 1493 Size: 636 2570 54 226 152 2109 1697 Fragments arranged by size: 2570 2109 1697 636 226 152 54 AseI AT'TA_AT Cuts at: 160 1032 2508 5998 160 Size: 872 1476 3490 1606 Fragments arranged by size: 3490 1606 1476 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7444 AvaI C'yCGr_G Cuts at: 1870 1943 4769 1870 Size: 73 2826 4545 Fragments arranged by size: 4545 2826 73 BamHI G'GATC_C Cuts at: 4774 5141 4774 Size: 367 7077 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3759 4765 6220 618 Size: 325 217 627 1972 1006 1455 1842 Fragments arranged by size: 1972 1842 1455 1006 627 325 217 BanII G_rGCy'C Cuts at: 1391 3756 4763 1391 Size: 2365 1007 4072 Fragments arranged by size: 4072 2365 1007 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2401 2849 3241 4461 928 Size: 971 502 448 392 1220 3911 Fragments arranged by size: 3911 1220 971 502 448 392 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5635 5669 1903 Size: 34 3698 34 3678 Fragments arranged by size: 3698 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5336 6546 5336 Size: 1210 6234 BclI T'GATC_A Cuts at: 1548 1548 Size: 7444 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 6055 136 Size: 107 122 71 631 605 4383 1525 Fragments arranged by size: 4383 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3153 7439 3153 Size: 4286 3158 BlpI GC'TnA_GC Cuts at: 3128 4133 4861 3128 Size: 1005 728 5711 Fragments arranged by size: 5711 1005 728 Bme1580I G_kGCm'C Cuts at: 1790 2854 5505 6661 1790 Size: 1064 2651 1156 2573 Fragments arranged by size: 2651 2573 1156 1064 BmgBI CAC'GTC Cuts at: 1318 2847 4210 1318 Size: 1529 1363 4552 Fragments arranged by size: 4552 1529 1363 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2194 2384 2878 Size: 416 53 83 399 24 777 190 494 Cuts at: 2878 3292 5175 6133 442 Size: 414 1883 958 1753 Fragments arranged by size: 1883 1753 958 777 494 416 414 399 190 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7444 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2090 2797 3044 3818 6104 1239 Size: 851 707 247 774 2286 2579 Fragments arranged by size: 2579 2286 851 774 707 247 Bpu10I CC'TnA_GC Cuts at: 2153 3242 2153 Size: 1089 6355 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2666 3776 3947 5569 6434 6732 2666 Size: 1110 171 1622 865 298 3378 Fragments arranged by size: 3378 1622 1110 865 298 171 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3224 3642 6107 882 Size: 617 1725 418 2465 2219 Fragments arranged by size: 2465 2219 1725 617 418 BsaAI yAC'GTr Cuts at: 493 1204 4331 4741 5235 493 Size: 711 3127 410 494 2702 Fragments arranged by size: 3127 2702 711 494 410 BsaBI GATnn'nnATC Cuts at: 3528 7438 3528 Size: 3910 3534 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2430 3027 3496 5251 Size: 53 83 186 936 897 597 469 1755 Cuts at: 5251 5633 6268 275 Size: 382 635 1451 Fragments arranged by size: 1755 1451 936 897 635 597 469 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4561 5877 6275 6549 6696 7107 4561 Size: 1316 398 274 147 411 4898 Fragments arranged by size: 4898 1316 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 7219 Fragments arranged by size: 7219 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2162 2846 3491 4082 1186 Size: 976 684 645 591 4548 Fragments arranged by size: 4548 976 684 645 591 BseYI C'CCAG_C Cuts at: 3825 6647 7143 3825 Size: 2822 496 4126 Fragments arranged by size: 4126 2822 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2057 2780 2882 2960 3194 4236 4367 Size: 573 321 723 102 78 234 1042 131 Cuts at: 4367 1163 Size: 4240 Fragments arranged by size: 4240 1042 723 573 321 234 131 102 78 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2315 4375 4894 5655 6683 1540 Size: 275 186 314 2060 519 761 1028 2301 Fragments arranged by size: 2301 2060 1028 761 519 314 275 186 BsiHKAI G_wGCw'C Cuts at: 2295 2854 3060 3417 3756 5505 5590 6661 2295 Size: 559 206 357 339 1749 85 1071 3078 Fragments arranged by size: 3078 1749 1071 559 357 339 206 85 BsmI GAATG_Cn' Cuts at: 4667 4955 5048 4667 Size: 288 93 7063 Fragments arranged by size: 7063 288 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3503 4041 4043 3503 Size: 538 2 6904 Fragments arranged by size: 6904 538 2 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2295 2854 3060 3417 3756 4763 5505 Size: 399 505 559 206 357 339 1007 742 Cuts at: 5505 5590 6661 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 1007 742 559 505 399 357 339 206 85 BspEI T'CCGG_A Cuts at: 7107 7107 Size: 7444 BspHI T'CATG_A Cuts at: 3693 5333 7063 3693 Size: 1640 1730 4074 Fragments arranged by size: 4074 1730 1640 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4799 844 Size: 3955 3489 BsrBI CCG'CTC Cuts at: 1394 1823 5331 1394 Size: 429 3508 3507 Fragments arranged by size: 3508 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2931 4729 5939 6113 65 Size: 1183 1683 1798 1210 174 1396 Fragments arranged by size: 1798 1683 1396 1210 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 6088 6275 1700 Size: 110 4278 187 2869 Fragments arranged by size: 4278 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2753 96 Size: 2657 4787 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7444 BssSI C'ACGA_G Cuts at: 2858 3406 5504 6516 2858 Size: 548 2098 1012 3786 Fragments arranged by size: 3786 2098 1012 548 BstAPI GCAn_nnn'nTGC Cuts at: 794 2665 3547 4278 4390 4579 794 Size: 1871 882 731 112 189 3659 Fragments arranged by size: 3659 1871 882 731 189 112 BstBI TT'CG_AA Cuts at: 2007 6248 2007 Size: 4241 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7444 BstYI r'GATC_y Cuts at: 1972 3153 4774 5141 5526 5543 6995 7081 7093 Size: 1181 1621 367 385 17 1452 86 12 Cuts at: 7093 7439 1972 Size: 346 1977 Fragments arranged by size: 1977 1621 1452 1181 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2022 3030 3234 4263 4428 513 Size: 552 957 1008 204 1029 165 3529 Fragments arranged by size: 3529 1029 1008 957 552 204 165 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6705 Fragments arranged by size: 6705 683 56 BtsI GCAGTG_nn' Cuts at: 2943 2988 4960 5754 5774 2943 Size: 45 1972 794 20 4613 Fragments arranged by size: 4613 1972 794 45 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7444 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7409 DraI TTT'AAA Cuts at: 4748 4748 Size: 7444 DraIII CAC_nnn'GTG Cuts at: 4303 4303 Size: 7444 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6451 809 Size: 507 5135 1802 Fragments arranged by size: 5135 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2312 2992 3120 Size: 54 1425 50 275 18 482 680 128 Cuts at: 3120 4891 5780 7409 8 Size: 1771 889 1629 43 Fragments arranged by size: 1771 1629 1425 889 680 482 275 128 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2312 4891 1537 Size: 275 500 2579 4090 Fragments arranged by size: 4090 2579 500 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2563 3853 3875 4234 4319 4337 5374 1353 Size: 1210 1290 22 359 85 18 1037 3423 Fragments arranged by size: 3423 1290 1210 1037 359 85 22 18 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2042 6031 6405 6551 387 Size: 1064 138 453 3989 374 146 1280 Fragments arranged by size: 3989 1280 1064 453 374 146 138 EcoICRI GAG'CTC Cuts at: 3754 3754 Size: 7444 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2090 2395 2607 2797 3044 3818 3977 Size: 393 458 305 212 190 247 774 159 Cuts at: 3977 4013 4260 5507 6104 6891 7159 1239 Size: 36 247 1247 597 787 268 1524 Fragments arranged by size: 1524 1247 787 774 597 458 393 305 268 247 247 212 190 159 36 EcoNI CCTnn'n_nnAGG Cuts at: 4169 4361 4169 Size: 192 7252 EcoO109I rG'GnC_Cy Cuts at: 3822 3931 3822 Size: 109 7335 EcoRI G'AATT_C Cuts at: 4699 4753 5131 4699 Size: 54 378 7012 Fragments arranged by size: 7012 378 54 EcoRV GAT'ATC Cuts at: 2587 2587 Size: 7444 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2208 3706 2208 Size: 1498 5946 FspI TGC'GCA Cuts at: 5950 5950 Size: 7444 HaeII r_GCGC'y Cuts at: 1678 2913 6591 1678 Size: 1235 3678 2531 Fragments arranged by size: 3678 2531 1235 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2723 2755 2970 3002 6085 Size: 32 135 32 1151 32 215 32 3083 Cuts at: 6085 6117 6159 6191 1373 Size: 32 42 32 2626 Fragments arranged by size: 3083 2626 1151 215 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4684 4788 5034 669 Size: 988 281 2746 104 246 3079 Fragments arranged by size: 3079 2746 988 281 246 104 HindIII A'AGCT_T Cuts at: 748 2228 3371 748 Size: 1480 1143 4821 Fragments arranged by size: 4821 1480 1143 HpaI GTT'AAC Cuts at: 5034 5034 Size: 7444 KpnI G_GTAC'C Cuts at: 3763 4769 3763 Size: 1006 6438 MfeI C'AATT_G Cuts at: 3747 5043 3747 Size: 1296 6148 MluI A'CGCG_T Cuts at: 7320 7320 Size: 7444 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3449 3471 3763 6558 6742 1305 Size: 2144 22 292 2795 184 2007 Fragments arranged by size: 2795 2144 2007 292 184 22 MscI TGG'CCA Cuts at: 10 64 1489 1832 3122 10 Size: 54 1425 343 1290 4332 Fragments arranged by size: 4332 1425 1290 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2936 5402 5761 5920 518 Size: 648 1770 2466 359 159 2042 Fragments arranged by size: 2466 2042 1770 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2074 2775 2812 3064 4324 4860 5537 6685 Size: 468 701 37 252 1260 536 677 1148 Cuts at: 6685 6930 1606 Size: 245 2120 Fragments arranged by size: 2120 1260 1148 701 677 536 468 252 245 37 NcoI C'CATG_G Cuts at: 513 1065 2022 3030 3234 4263 513 Size: 552 957 1008 204 1029 3694 Fragments arranged by size: 3694 1029 1008 957 552 204 NdeI CA'TA_TG Cuts at: 387 4179 387 Size: 3792 3652 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7444 NotI GC'GGCC_GC Cuts at: 4891 4891 Size: 7444 NruI TCG'CGA Cuts at: 2568 2568 Size: 7444 NspI r_CATG'y Cuts at: 1132 1229 2220 3463 4802 6347 1132 Size: 97 991 1243 1339 1545 2229 Fragments arranged by size: 2229 1545 1339 1243 991 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7444 PciI A'CATG_T Cuts at: 6343 6343 Size: 7444 PflMI CCAn_nnn'nTGG Cuts at: 1108 2206 2775 4493 1108 Size: 1098 569 1718 4059 Fragments arranged by size: 4059 1718 1098 569 PmeI GTTT'AAAC Cuts at: 4748 4748 Size: 7444 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5513 5545 7026 7058 5513 Size: 32 1481 32 5899 Fragments arranged by size: 5899 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3822 3931 3822 Size: 109 7335 PshAI GACnn'nnGTC Cuts at: 1613 3236 1613 Size: 1623 5821 PsiI TTA'TAA Cuts at: 5014 5014 Size: 7444 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 3527 3559 3527 Size: 32 7412 PstI C_TGCA'G Cuts at: 830 4074 4796 830 Size: 3244 722 3478 Fragments arranged by size: 3478 3244 722 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7444 PvuII CAG'CTG Cuts at: 2074 2775 2812 3064 4324 2074 Size: 701 37 252 1260 5194 Fragments arranged by size: 5194 1260 701 252 37 SacI G_AGCT'C Cuts at: 3756 3756 Size: 7444 SalI G'TCGA_C Cuts at: 1936 4786 1936 Size: 2850 4594 SbfI CC_TGCA'GG Cuts at: 4796 4796 Size: 7444 ScaI AGT'ACT Cuts at: 3281 5692 7404 3281 Size: 2411 1712 3321 Fragments arranged by size: 3321 2411 1712 SfcI C'TryA_G Cuts at: 826 1045 2903 3317 4070 4459 4792 5927 6608 Size: 219 1858 414 753 389 333 1135 681 Cuts at: 6608 6799 826 Size: 191 1471 Fragments arranged by size: 1858 1471 1135 753 681 414 389 333 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7444 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7444 SmaI CCC'GGG Cuts at: 1872 4771 1872 Size: 2899 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2681 3791 3962 5548 6449 6711 Size: 197 926 738 1110 171 1586 901 262 Cuts at: 6711 820 Size: 1553 Fragments arranged by size: 1586 1553 1110 926 901 738 262 197 171 SnaBI TAC'GTA Cuts at: 493 4741 5235 493 Size: 4248 494 2702 Fragments arranged by size: 4248 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 7444 SphI G_CATG'C Cuts at: 1132 4802 1132 Size: 3670 3774 SspI AAT'ATT Cuts at: 5 52 5368 5 Size: 47 5316 2081 Fragments arranged by size: 5316 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7444 StyI C'CwwG_G Cuts at: 513 1065 2022 2121 3030 3234 4263 4883 513 Size: 552 957 99 909 204 1029 620 3074 Fragments arranged by size: 3074 1029 957 909 620 552 204 99 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2540 4667 5471 5488 5641 5826 6271 Size: 1255 960 2127 804 17 153 185 445 Cuts at: 6271 325 Size: 1498 Fragments arranged by size: 2127 1498 1255 960 804 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2753 3279 3776 Size: 274 80 33 51 1443 776 526 497 Cuts at: 3776 3787 5690 7402 96 Size: 11 1903 1712 138 Fragments arranged by size: 1903 1712 1443 776 526 497 274 138 80 51 33 11 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2512 2544 7404 7436 2512 Size: 32 4860 32 2520 Fragments arranged by size: 4860 2520 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2405 1316 Size: 1089 6355 XbaI T'CTAG_A Cuts at: 4780 4780 Size: 7444 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7444 XmaI C'CCGG_G Cuts at: 1870 4769 1870 Size: 2899 4545 XmnI GAAnn'nnTTC Cuts at: 3144 3810 5573 3144 Size: 666 1763 5015 Fragments arranged by size: 5015 1763 666 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2431 5252 276 Size: 53 83 186 936 897 2821 2468 Fragments arranged by size: 2821 2468 936 897 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NruI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PsrI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BstXI BstZ17I Bsu36I FseI FspAI KasI NaeI NarI NgoMIV NsiI PacI PfoI PmlI PspOMI PspXI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI