Restriction map for
pFN21ASDA0745
(Linear) (Six-base) MAPSORT of: KIAA0745.seq Check: 1906 from: 1 to: 2715
KIAA0745.orf
With 182 enzymes: SgfI *
October 24, 2006 15:13 ..
AatII G_ACGT'C
Cuts at: 0 410 2715
Size: 410 2305
Acc65I G'GTAC_C
Cuts at: 0 1736 2715
Size: 1736 979
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 372 584 1954 1990 2237 2715
Size: 372 212 1370 36 247 478
Fragments arranged by size:
1370 478 372 247 212 36
AflIII A'CryG_T
Cuts at: 0 823 2715
Size: 823 1892
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 210 2715
Size: 210 2505
AlwNI CAG_nnn'CTG
Cuts at: 0 21 183 876 2400 2637 2715
Size: 21 162 693 1524 237 78
Fragments arranged by size:
1524 693 237 162 78 21
ApaLI G'TGCA_C
Cuts at: 0 827 2715
Size: 827 1888
ApoI r'AATT_y
Cuts at: 0 106 2676 2715
Size: 106 2570 39
Fragments arranged by size:
2570 106 39
AseI AT'TA_AT
Cuts at: 0 485 2715
Size: 485 2230
BanI G'GyrC_C
Cuts at: 0 1736 2715
Size: 1736 979
BanII G_rGCy'C
Cuts at: 0 1733 2715
Size: 1733 982
BbsI GAAGACnn'nnnn_
Cuts at: 0 378 826 1218 2438 2715
Size: 378 448 392 1220 277
Fragments arranged by size:
1220 448 392 378 277
BglII A'GATC_T
Cuts at: 0 1130 2715
Size: 1130 1585
BlpI GC'TnA_GC
Cuts at: 0 1105 2110 2715
Size: 1105 1005 605
Fragments arranged by size:
1105 1005 605
Bme1580I G_kGCm'C
Cuts at: 0 831 2715
Size: 831 1884
BmgBI CAC'GTC
Cuts at: 0 824 2187 2715
Size: 824 1363 528
Fragments arranged by size:
1363 824 528
BmrI ACTGGGnnnn_n'
Cuts at: 0 171 361 855 1269 2715
Size: 171 190 494 414 1446
Fragments arranged by size:
1446 494 414 190 171
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 67 774 1021 1795 2715
Size: 67 707 247 774 920
Fragments arranged by size:
920 774 707 247 67
Bpu10I CC'TnA_GC
Cuts at: 0 130 1219 2715
Size: 130 1089 1496
Fragments arranged by size:
1496 1089 130
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 643 1753 1924 2715
Size: 643 1110 171 791
Fragments arranged by size:
1110 791 643 171
BsaI GGTCTCn'nnnn_
Cuts at: 0 1201 1619 2715
Size: 1201 418 1096
Fragments arranged by size:
1201 1096 418
BsaAI yAC'GTr
Cuts at: 0 2308 2715
Size: 2308 407
BsaBI GATnn'nnATC
Cuts at: 0 1505 2715
Size: 1505 1210
BsaHI Gr'CG_yC
Cuts at: 0 407 1004 1473 2715
Size: 407 597 469 1242
Fragments arranged by size:
1242 597 469 407
BsaWI w'CCGG_w
Cuts at: 0 2538 2715
Size: 2538 177
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 139 823 1468 2059 2715
Size: 139 684 645 591 656
Fragments arranged by size:
684 656 645 591 139
BseYI C'CCAG_C
Cuts at: 0 1802 2715
Size: 1802 913
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 34 757 859 937 1171 2213 2344 2715
Size: 34 723 102 78 234 1042 131 371
Fragments arranged by size:
1042 723 371 234 131 102 78 34
BsiEI CG_ry'CG
Cuts at: 0 292 2352 2715
Size: 292 2060 363
Fragments arranged by size:
2060 363 292
BsiHKAI G_wGCw'C
Cuts at: 0 272 831 1037 1394 1733 2715
Size: 272 559 206 357 339 982
Fragments arranged by size:
982 559 357 339 272 206
BsmI GAATG_Cn'
Cuts at: 0 2644 2715
Size: 2644 71
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1480 2018 2020 2715
Size: 1480 538 2 695
Fragments arranged by size:
1480 695 538 2
Bsp1286I G_dGCh'C
Cuts at: 0 272 831 1037 1394 1733 2715
Size: 272 559 206 357 339 982
Fragments arranged by size:
982 559 357 339 272 206
BspHI T'CATG_A
Cuts at: 0 1670 2715
Size: 1670 1045
BsrDI GCAATG_nn'
Cuts at: 0 908 2706 2715
Size: 908 1798 9
Fragments arranged by size:
1798 908 9
BsrGI T'GTAC_A
Cuts at: 0 730 2715
Size: 730 1985
BssSI C'ACGA_G
Cuts at: 0 835 1383 2715
Size: 835 548 1332
Fragments arranged by size:
1332 835 548
BstAPI GCAn_nnn'nTGC
Cuts at: 0 642 1524 2255 2367 2556 2715
Size: 642 882 731 112 189 159
Fragments arranged by size:
882 731 642 189 159 112
BstYI r'GATC_y
Cuts at: 0 1130 2715
Size: 1130 1585
BtgI C'CryG_G
Cuts at: 0 1007 1211 2240 2405 2715
Size: 1007 204 1029 165 310
Fragments arranged by size:
1029 1007 310 204 165
BtsI GCAGTG_nn'
Cuts at: 0 920 965 2715
Size: 920 45 1750
Fragments arranged by size:
1750 920 45
DraIII CAC_nnn'GTG
Cuts at: 0 2280 2715
Size: 2280 435
EaeI y'GGCC_r
Cuts at: 0 289 969 1097 2715
Size: 289 680 128 1618
Fragments arranged by size:
1618 680 289 128
EagI C'GGCC_G
Cuts at: 0 289 2715
Size: 289 2426
EarI CTCTTCn'nnn_
Cuts at: 0 540 1830 1852 2211 2296 2314 2715
Size: 540 1290 22 359 85 18 401
Fragments arranged by size:
1290 540 401 359 85 22 18
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 19 2715
Size: 19 2696
EcoICRI GAG'CTC
Cuts at: 0 1731 2715
Size: 1731 984
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 67 372 584 774 1021 1795 1954 1990
Size: 67 305 212 190 247 774 159 36
Cuts at: 1990 2237 2715
Size: 247 478
Fragments arranged by size:
774 478 305 247 247 212 190 159
67 36
EcoNI CCTnn'n_nnAGG
Cuts at: 0 2146 2338 2715
Size: 2146 192 377
Fragments arranged by size:
2146 377 192
EcoO109I rG'GnC_Cy
Cuts at: 0 1799 1908 2715
Size: 1799 109 807
Fragments arranged by size:
1799 807 109
EcoRI G'AATT_C
Cuts at: 0 2676 2715
Size: 2676 39
EcoRV GAT'ATC
Cuts at: 0 564 2715
Size: 564 2151
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 185 1683 2715
Size: 185 1498 1032
Fragments arranged by size:
1498 1032 185
HaeII r_GCGC'y
Cuts at: 0 890 2715
Size: 890 1825
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 700 732 947 979 2715
Size: 700 32 215 32 1736
Fragments arranged by size:
1736 700 215 32 32
HincII GTy'rAC
Cuts at: 0 2661 2715
Size: 2661 54
HindIII A'AGCT_T
Cuts at: 0 205 1348 2715
Size: 205 1143 1367
Fragments arranged by size:
1367 1143 205
KpnI G_GTAC'C
Cuts at: 0 1740 2715
Size: 1740 975
MfeI C'AATT_G
Cuts at: 0 1724 2715
Size: 1724 991
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1426 1448 1740 2715
Size: 1426 22 292 975
Fragments arranged by size:
1426 975 292 22
MscI TGG'CCA
Cuts at: 0 1099 2715
Size: 1099 1616
MslI CAynn'nnrTG
Cuts at: 0 913 2715
Size: 913 1802
MspA1I CmG'CkG
Cuts at: 0 51 752 789 1041 2301 2715
Size: 51 701 37 252 1260 414
Fragments arranged by size:
1260 701 414 252 51 37
NcoI C'CATG_G
Cuts at: 0 1007 1211 2240 2715
Size: 1007 204 1029 475
Fragments arranged by size:
1029 1007 475 204
NdeI CA'TA_TG
Cuts at: 0 2156 2715
Size: 2156 559
NruI TCG'CGA
Cuts at: 0 545 2715
Size: 545 2170
NspI r_CATG'y
Cuts at: 0 197 1440 2715
Size: 197 1243 1275
Fragments arranged by size:
1275 1243 197
PflMI CCAn_nnn'nTGG
Cuts at: 0 183 752 2470 2715
Size: 183 569 1718 245
Fragments arranged by size:
1718 569 245 183
PpuMI rG'GwC_Cy
Cuts at: 0 1799 1908 2715
Size: 1799 109 807
Fragments arranged by size:
1799 807 109
PshAI GACnn'nnGTC
Cuts at: 0 1213 2715
Size: 1213 1502
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1504 1536 2715
Size: 1504 32 1179
Fragments arranged by size:
1504 1179 32
PstI C_TGCA'G
Cuts at: 0 2051 2715
Size: 2051 664
PvuII CAG'CTG
Cuts at: 0 51 752 789 1041 2301 2715
Size: 51 701 37 252 1260 414
Fragments arranged by size:
1260 701 414 252 51 37
SacI G_AGCT'C
Cuts at: 0 1733 2715
Size: 1733 982
ScaI AGT'ACT
Cuts at: 0 1258 2715
Size: 1258 1457
SfcI C'TryA_G
Cuts at: 0 880 1294 2047 2436 2715
Size: 880 414 753 389 279
Fragments arranged by size:
880 753 414 389 279
SmlI C'TyrA_G
Cuts at: 0 658 1768 1939 2715
Size: 658 1110 171 776
Fragments arranged by size:
1110 776 658 171
StyI C'CwwG_G
Cuts at: 0 98 1007 1211 2240 2715
Size: 98 909 204 1029 475
Fragments arranged by size:
1029 909 475 204 98
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 517 2644 2715
Size: 517 2127 71
Fragments arranged by size:
2127 517 71
TatI w'GTAC_w
Cuts at: 0 730 1256 1753 1764 2715
Size: 730 526 497 11 951
Fragments arranged by size:
951 730 526 497 11
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 489 521 2715
Size: 489 32 2194
Fragments arranged by size:
2194 489 32
Tth111I GACn'n_nGTC
Cuts at: 0 382 2715
Size: 382 2333
XmnI GAAnn'nnTTC
Cuts at: 0 1121 1787 2715
Size: 1121 666 928
Fragments arranged by size:
1121 928 666
ZraI GAC'GTC
Cuts at: 0 408 2715
Size: 408 2307
Enzymes that do cut:
AatII Acc65I AcuI AflIII AlfI AlwNI ApaLI ApoI
AseI BanI BanII BbsI BglII BlpI Bme1580I BmgBI
BmrI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspHI BsrDI BsrGI BssSI BstAPI BstYI BtgI
BtsI DraIII EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI EcoRV FalI HaeII Hin4I HincII
HindIII KpnI MfeI MmeI MscI MslI MspA1I NcoI
NdeI NruI NspI PflMI PpuMI PshAI PsrI PstI
PvuII SacI ScaI SfcI SmlI StyI TaqII TatI
TstI Tth111I XmnI ZraI
Enzymes that do not cut:
AarI AccI AclI AfeI AflII AgeI AhdI AleI
AloI ApaI AscI AsiSI AvaI AvrII BaeI BamHI
BbeI BbvCI BcgI BciVI BclI BfrBI BglI BmtI
BplI BsaXI BsiWI BspEI BspMI BsrBI BsrFI BssHII
BstBI BstEII BstXI BstZ17I Bsu36I BtgZI ClaI CspCI
DraI DrdI FseI FspI FspAI HpaI KasI MluI
NaeI NarI NgoMIV NheI NotI NsiI PacI PasI
PciI PfoI PmeI PmlI PpiI PsiI PspOMI PspXI
PvuI RsrII SacII SalI SanDI SapI SbfI SexAI
SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI
SrfI SspI StuI SwaI XbaI XcmI XhoI XmaI