(Circular) (Six-base) MAPSORT of: pFN21ASDA0735.seq Check: 658 from: 1 to: 6778 pFN21ASDA0735 6778 bp With 182 enzymes: SgfI * January 16, 2009 15:30 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4588 278 Size: 53 83 186 936 3052 2468 Fragments arranged by size: 3052 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2484 4121 1937 Size: 547 1637 4594 Fragments arranged by size: 4594 1637 547 Acc65I G'GTAC_C Cuts at: 3099 4099 3099 Size: 1000 5778 AclI AA'CG_TT Cuts at: 1563 4905 5278 1563 Size: 3342 373 3063 Fragments arranged by size: 3342 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2800 2924 4841 6225 6493 1632 Size: 1168 124 1917 1384 268 1917 Fragments arranged by size: 1917 1917 1384 1168 268 124 AfeI AGC'GCT Cuts at: 3107 3107 Size: 6778 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6581 AflIII A'CryG_T Cuts at: 1203 2439 3418 5677 6654 1203 Size: 1236 979 2259 977 1327 Fragments arranged by size: 2259 1327 1236 979 977 AgeI A'CCGG_T Cuts at: 5609 5609 Size: 6778 AhdI GACnn_n'nnGTC Cuts at: 5507 5507 Size: 6778 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6778 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3775 1184 Size: 2591 4187 AlwNI CAG_nnn'CTG Cuts at: 3814 4066 4521 6093 3814 Size: 252 455 1572 4499 Fragments arranged by size: 4499 1572 455 252 ApaI G_GGCC'C Cuts at: 3177 3177 Size: 6778 ApaLI G'TGCA_C Cuts at: 4835 5991 4835 Size: 1156 5622 ApoI r'AATT_y Cuts at: 1493 2696 3065 3926 4087 4313 4465 6574 1493 Size: 1203 369 861 161 226 152 2109 1697 Fragments arranged by size: 2109 1697 1203 861 369 226 161 152 AseI AT'TA_AT Cuts at: 160 1032 5332 160 Size: 872 4300 1606 Fragments arranged by size: 4300 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6778 AvaI C'yCGr_G Cuts at: 1870 1943 2706 3674 4053 4103 1870 Size: 73 763 968 379 50 4545 Fragments arranged by size: 4545 968 763 379 73 50 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2161 2194 2161 Size: 33 6745 BamHI G'GATC_C Cuts at: 2349 4108 4475 2349 Size: 1759 367 4652 Fragments arranged by size: 4652 1759 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2221 2504 3099 3332 3932 Size: 325 217 627 434 283 595 233 600 Cuts at: 3932 4099 5554 618 Size: 167 1455 1842 Fragments arranged by size: 1842 1455 627 600 595 434 325 283 233 217 167 BanII G_rGCy'C Cuts at: 1391 2924 3177 4097 1391 Size: 1533 253 920 4072 Fragments arranged by size: 4072 1533 920 253 BbeI G_GCGC'C Cuts at: 2225 2508 3336 3936 2225 Size: 283 828 600 5067 Fragments arranged by size: 5067 828 600 283 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2154 3468 928 Size: 971 255 1314 4238 Fragments arranged by size: 4238 1314 971 255 BbvCI CC'TCA_GC Cuts at: 2555 2555 Size: 6778 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4969 5003 1903 Size: 34 3032 34 3678 Fragments arranged by size: 3678 3032 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2189 3885 4670 5880 2189 Size: 1696 785 1210 3087 Fragments arranged by size: 3087 1696 1210 785 BclI T'GATC_A Cuts at: 1548 3858 1548 Size: 2310 4468 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2210 2219 2247 Size: 107 122 71 631 605 538 9 28 Cuts at: 2247 2381 5389 136 Size: 134 3008 1525 Fragments arranged by size: 3008 1525 631 605 538 134 122 107 71 28 9 BglII A'GATC_T Cuts at: 2154 6773 2154 Size: 4619 2159 BlpI GC'TnA_GC Cuts at: 4195 4195 Size: 6778 Bme1580I G_kGCm'C Cuts at: 1790 3177 4839 5995 1790 Size: 1387 1662 1156 2573 Fragments arranged by size: 2573 1662 1387 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6778 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2061 2418 2766 Size: 416 53 83 399 24 644 357 348 Cuts at: 2766 4509 5467 442 Size: 1743 958 1753 Fragments arranged by size: 1753 1743 958 644 416 399 357 348 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6778 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2642 2744 2642 Size: 102 6676 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2212 2806 2869 3602 5438 1239 Size: 973 594 63 733 1836 2579 Fragments arranged by size: 2579 1836 973 733 594 63 Bpu10I CC'TnA_GC Cuts at: 2555 3003 2555 Size: 448 6330 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2957 3704 4903 5768 6066 2957 Size: 747 1199 865 298 3669 Fragments arranged by size: 3669 1199 865 747 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2303 5441 882 Size: 617 804 3138 2219 Fragments arranged by size: 3138 2219 804 617 BsaAI yAC'GTr Cuts at: 493 1204 4075 4569 493 Size: 711 2871 494 2702 Fragments arranged by size: 2871 2702 711 494 BsaBI GATnn'nnATC Cuts at: 6772 6772 Size: 6778 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2222 2505 3333 3933 Size: 53 83 186 936 689 283 828 600 Cuts at: 3933 4585 4967 5602 275 Size: 652 382 635 1451 Fragments arranged by size: 1451 936 828 689 652 635 600 382 283 186 83 53 BsaWI w'CCGG_w Cuts at: 5211 5609 5883 6030 6441 5211 Size: 398 274 147 411 5548 Fragments arranged by size: 5548 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6553 Fragments arranged by size: 6553 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2300 2733 1186 Size: 1114 433 5231 Fragments arranged by size: 5231 1114 433 BseYI C'CCAG_C Cuts at: 2536 2737 2808 5981 6477 2536 Size: 201 71 3173 496 2837 Fragments arranged by size: 3173 2837 496 201 71 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2448 2627 2866 1163 Size: 573 712 179 239 5075 Fragments arranged by size: 5075 712 573 239 179 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4228 4989 6017 1540 Size: 275 186 2227 761 1028 2301 Fragments arranged by size: 2301 2227 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2668 2875 3543 4839 4924 5995 2668 Size: 207 668 1296 85 1071 3451 Fragments arranged by size: 3451 1296 1071 668 207 85 BsmI GAATG_Cn' Cuts at: 2472 3224 3850 3906 4289 4382 2472 Size: 752 626 56 383 93 4868 Fragments arranged by size: 4868 752 626 383 93 56 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2668 2875 2924 3177 3543 4097 4839 Size: 399 878 207 49 253 366 554 742 Cuts at: 4839 4924 5995 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 878 742 554 399 366 253 207 85 49 BspEI T'CCGG_A Cuts at: 6441 6441 Size: 6778 BspHI T'CATG_A Cuts at: 4667 6397 4667 Size: 1730 5048 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3130 4133 844 Size: 2286 1003 3489 Fragments arranged by size: 3489 2286 1003 BsrBI CCG'CTC Cuts at: 1394 1823 4665 1394 Size: 429 2842 3507 Fragments arranged by size: 3507 2842 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2095 3801 3831 5273 5447 65 Size: 1183 847 1706 30 1442 174 1396 Fragments arranged by size: 1706 1442 1396 1183 847 174 30 BsrFI r'CCGG_y Cuts at: 1700 1810 5422 5609 1700 Size: 110 3612 187 2869 Fragments arranged by size: 3612 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6778 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6778 BssSI C'ACGA_G Cuts at: 4838 5850 4838 Size: 1012 5766 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6778 BstBI TT'CG_AA Cuts at: 2007 5582 2007 Size: 3575 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6778 BstXI CCAn_nnnn'nTGG Cuts at: 3058 3374 3580 3997 3058 Size: 316 206 417 5839 Fragments arranged by size: 5839 417 316 206 BstYI r'GATC_y Cuts at: 1972 2154 2349 3075 3363 4108 4475 4860 4877 Size: 182 195 726 288 745 367 385 17 Cuts at: 4877 6329 6415 6427 6773 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 745 726 385 367 346 288 195 182 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2022 2083 2332 2778 2892 3204 3219 Size: 552 957 61 249 446 114 312 15 Cuts at: 3219 513 Size: 4072 Fragments arranged by size: 4072 957 552 446 312 249 114 61 15 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6039 Fragments arranged by size: 6039 683 56 BtsI GCAGTG_nn' Cuts at: 3815 4294 5088 5108 3815 Size: 479 794 20 5485 Fragments arranged by size: 5485 794 479 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6778 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6743 DraI TTT'AAA Cuts at: 3415 4082 3415 Size: 667 6111 DraIII CAC_nnn'GTG Cuts at: 2120 2120 Size: 6778 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5785 809 Size: 507 4469 1802 Fragments arranged by size: 4469 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2329 2335 2382 Size: 54 1425 50 275 18 499 6 47 Cuts at: 2382 2568 3201 4225 5114 6743 8 Size: 186 633 1024 889 1629 43 Fragments arranged by size: 1629 1425 1024 889 633 499 275 186 54 50 47 43 18 6 EagI C'GGCC_G Cuts at: 1537 1812 4225 1537 Size: 275 2413 4090 Fragments arranged by size: 4090 2413 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2602 2633 4708 1353 Size: 1249 31 2075 3423 Fragments arranged by size: 3423 2075 1249 31 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5365 5739 5885 387 Size: 1064 138 3776 374 146 1280 Fragments arranged by size: 3776 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2212 2800 2806 2869 2924 3602 4841 Size: 393 580 588 6 63 55 678 1239 Cuts at: 4841 5438 6225 6493 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 1239 787 678 597 588 580 393 268 63 55 6 EcoO109I rG'GnC_Cy Cuts at: 2761 3009 3173 3174 4063 2761 Size: 248 164 1 889 5476 Fragments arranged by size: 5476 889 248 164 1 EcoRI G'AATT_C Cuts at: 2696 3065 4087 4465 2696 Size: 369 1022 378 5009 Fragments arranged by size: 5009 1022 378 369 FspI TGC'GCA Cuts at: 5284 5284 Size: 6778 HaeII r_GCGC'y Cuts at: 1678 2225 2508 3109 3336 3936 5925 1678 Size: 547 283 601 227 600 1989 2531 Fragments arranged by size: 2531 1989 601 600 547 283 227 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2844 2876 3343 3375 3455 Size: 32 135 32 1272 32 467 32 80 Cuts at: 3455 3487 4047 4079 5419 5451 5493 5525 1373 Size: 32 560 32 1340 32 42 32 2626 Fragments arranged by size: 2626 1340 1272 560 467 135 80 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4122 4368 669 Size: 988 281 2184 246 3079 Fragments arranged by size: 3079 2184 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6778 HpaI GTT'AAC Cuts at: 4368 4368 Size: 6778 KasI G'GCGC_C Cuts at: 2221 2504 3332 3932 2221 Size: 283 828 600 5067 Fragments arranged by size: 5067 828 600 283 KpnI G_GTAC'C Cuts at: 3103 4103 3103 Size: 1000 5778 MfeI C'AATT_G Cuts at: 4377 4377 Size: 6778 MluI A'CGCG_T Cuts at: 6654 6654 Size: 6778 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2121 3060 5892 6076 1305 Size: 816 939 2832 184 2007 Fragments arranged by size: 2832 2007 939 816 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2331 10 Size: 54 1425 343 499 4457 Fragments arranged by size: 4457 1425 499 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2190 2993 3372 3404 4736 5095 5254 Size: 648 1024 803 379 32 1332 359 159 Cuts at: 5254 518 Size: 2042 Fragments arranged by size: 2042 1332 1024 803 648 379 359 159 32 MspA1I CmG'CkG Cuts at: 1606 2085 4194 4871 6019 6264 1606 Size: 479 2109 677 1148 245 2120 Fragments arranged by size: 2120 2109 1148 677 479 245 NarI GG'CG_CC Cuts at: 2222 2505 3333 3933 2222 Size: 283 828 600 5067 Fragments arranged by size: 5067 828 600 283 NcoI C'CATG_G Cuts at: 513 1065 2022 2332 2778 2892 3219 513 Size: 552 957 310 446 114 327 4072 Fragments arranged by size: 4072 957 552 446 327 310 114 NdeI CA'TA_TG Cuts at: 387 387 Size: 6778 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6778 NotI GC'GGCC_GC Cuts at: 4225 4225 Size: 6778 NspI r_CATG'y Cuts at: 1132 1229 2443 2563 3326 3422 4136 5681 1132 Size: 97 1214 120 763 96 714 1545 2229 Fragments arranged by size: 2229 1545 1214 763 714 120 97 96 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6778 PciI A'CATG_T Cuts at: 2439 3418 5677 2439 Size: 979 2259 3540 Fragments arranged by size: 3540 2259 979 PflMI CCAn_nnn'nTGG Cuts at: 1108 2784 2804 1108 Size: 1676 20 5082 Fragments arranged by size: 5082 1676 20 PfoI T'CCnGG_A Cuts at: 3579 3579 Size: 6778 PmeI GTTT'AAAC Cuts at: 4082 4082 Size: 6778 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3008 3040 3530 3562 4847 4879 6360 6392 3008 Size: 32 490 32 1285 32 1481 32 3394 Fragments arranged by size: 3394 1481 1285 490 32 32 32 32 PpuMI rG'GwC_Cy Cuts at: 3009 4063 3009 Size: 1054 5724 PshAI GACnn'nnGTC Cuts at: 1613 2442 1613 Size: 829 5949 PsiI TTA'TAA Cuts at: 4348 4348 Size: 6778 PspOMI G'GGCC_C Cuts at: 3173 3173 Size: 6778 PstI C_TGCA'G Cuts at: 830 3754 4130 830 Size: 2924 376 3478 Fragments arranged by size: 3478 2924 376 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6778 SacII CC_GC'GG Cuts at: 2086 2086 Size: 6778 SalI G'TCGA_C Cuts at: 1936 4120 1936 Size: 2184 4594 SanDI GG'GwC_CC Cuts at: 3009 3009 Size: 6778 SbfI CC_TGCA'GG Cuts at: 4130 4130 Size: 6778 ScaI AGT'ACT Cuts at: 5026 6738 5026 Size: 1712 5066 SexAI A'CCwGG_T Cuts at: 3095 3095 Size: 6778 SfcI C'TryA_G Cuts at: 826 1045 3178 3750 4126 5261 5942 6133 826 Size: 219 2133 572 376 1135 681 191 1471 Fragments arranged by size: 2133 1471 1135 681 572 376 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 2210 2381 2210 Size: 171 6607 SfoI GGC'GCC Cuts at: 2223 2506 3334 3934 2223 Size: 283 828 600 5067 Fragments arranged by size: 5067 828 600 283 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6778 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6778 SmaI CCC'GGG Cuts at: 1872 3676 4105 1872 Size: 1804 429 4545 Fragments arranged by size: 4545 1804 429 SmlI C'TyrA_G Cuts at: 820 1017 1943 2972 3719 4053 4882 5783 6045 Size: 197 926 1029 747 334 829 901 262 Cuts at: 6045 820 Size: 1553 Fragments arranged by size: 1553 1029 926 901 829 747 334 262 197 SnaBI TAC'GTA Cuts at: 493 4075 4569 493 Size: 3582 494 2702 Fragments arranged by size: 3582 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 6778 SphI G_CATG'C Cuts at: 1132 4136 1132 Size: 3004 3774 SspI AAT'ATT Cuts at: 5 52 4702 5 Size: 47 4650 2081 Fragments arranged by size: 4650 2081 47 StuI AGG'CCT Cuts at: 1517 2523 1517 Size: 1006 5772 StyI C'CwwG_G Cuts at: 513 1065 2022 2332 2778 2892 2924 3219 4217 Size: 552 957 310 446 114 32 295 998 Cuts at: 4217 513 Size: 3074 Fragments arranged by size: 3074 998 957 552 446 310 295 114 32 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2202 4805 4822 4975 5160 5605 325 Size: 1255 622 2603 17 153 185 445 1498 Fragments arranged by size: 2603 1498 1255 622 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3159 3408 3421 Size: 274 80 33 51 1443 1182 249 13 Cuts at: 3421 5024 6736 96 Size: 1603 1712 138 Fragments arranged by size: 1712 1603 1443 1182 274 249 138 80 51 33 13 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6738 6770 6738 Size: 32 6746 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6778 XbaI T'CTAG_A Cuts at: 4114 4114 Size: 6778 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2805 2805 Size: 6778 XhoI C'TCGA_G Cuts at: 1943 4053 1943 Size: 2110 4668 XmaI C'CCGG_G Cuts at: 1870 3674 4103 1870 Size: 1804 429 4545 Fragments arranged by size: 4545 1804 429 XmnI GAAnn'nnTTC Cuts at: 3023 4907 3023 Size: 1884 4894 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4586 276 Size: 53 83 186 936 3052 2468 Fragments arranged by size: 3052 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SacII SalI SanDI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI AscI AvrII BfrBI BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoNI EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI PspXI PsrI PvuII RsrII SacI SapI SrfI SwaI