Restriction map for
pFN21ASDA0723
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# NO CUT ENZYMES ( 123 enzymes) #
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AatII Acc65I AccII AccIII AclI
AcyI AflII AgeI AhdI AocI
Aor51HI ApaI AscI Asp718I AspEI
AvaI AviII BamHI BanI BbeI
BbrPI BbvCI BcgI BciVI BcnI
BfrI BglI BsaAI BsaBI BsaHI
BseAI BsiEI BsiWI BsoBI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrBI
BsrFI BsrGI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstUI BstXI
BstZ17I Bsu36I Cfr10I ClaI CpoI
DraI DraIII DrdI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoO65I
EcoRV EcoT22I FauI FseI FspI
HgaI Hin1I HpaI KasI KpnI
KspI MamI MfeI MluI MroI
MunI MvnI NaeI NarI NciI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PinAI
PmaCI PmeI PmlI PshAI Psp1406I
PstI PvuI RcaI RsrII SacII
SalI SapI SbfI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI SwaI XbaI
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AccI 1041 GTCTAC GT^[AC][GT]AC
AflIII 1485 ACGTGT A^C[GA][CT]GT
Alw44I 212 GTGCAC G^TGCAC
AlwNI 2228 CAGGTGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 212 GTGCAC G^TGCAC
AseI 1300 ATTAAT AT^TAAT
AsnI 1300 ATTAAT AT^TAAT
AspI 761 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 520 CCTAGG C^CTAGG
BalI 335 TGGCCA TGG^CCA
BanII 203 GAGCTC G[GA]GC[CT]^C
BlnI 520 CCTAGG C^CTAGG
BlpI 1549 GCTGAGC GC^T[ACGT]AGC
BpmI 1793 CTCCAG CTGGAG(16/14)
Bpu1102I 1549 GCTGAGC GC^T[ACGT]AGC
BsaWI 1891 TCCGGT [AT]^CCGG[AT]
BsgI 146 CTGCAC GTGCAG(16/14)
BsmBI 2027 GAGACG CGTCTC(1/5)
BsmI 1512 GCATTC GAATGC(1/-1)
BspMI 1145 ACCTGC ACCTGC(4/8)
BstAPI 148 GCACGCCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CelII 1549 GCTGAGC GC^T[ACGT]AGC
CfoI 812 GCGC GCG^C
EaeI 335 TGGCCA [CT]^GGCC[GA]
EcoNI 2389 CCTAAAACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 1061 GAATTC G^AATTC
HaeII 811 GGCGCT [GA]GCGC^[CT]
HapII 1892 CCGG C^CGG
HhaI 812 GCGC GCG^C
HinP1I 812 GCGC G^CGC
HpaII 1892 CCGG C^CGG
MluNI 335 TGGCCA TGG^CCA
MscI 335 TGGCCA TGG^CCA
MspA1I 1249 CAGCTG C[AC]G^C[GT]G
MspI 1892 CCGG C^CGG
NspI 1163 ACATGC [GA]CATG^[CT]
PflFI 761 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 1102 CCAGCAGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshBI 1300 ATTAAT AT^TAAT
PvuII 1249 CAGCTG CAG^CTG
SacI 203 GAGCTC GAGCT^C
ScaI 229 AGTACT AGT^ACT
SexAI 2226 ACCAGGT A^CC[AT]GGT
SfcI 427 CTACAG C^T[GA][CT]AG
SspI 829 AATATT AAT^ATT
StuI 78 AGGCCT AGG^CCT
Tth111I 761 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 1102 CCAGCAGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 323 CCAGTAACATTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 14 enzymes) #
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AlwI 1021 GATCC GGATC(4/5)
2257 GATCC
BclI 1464 TGATCA T^GATCA
2169 TGATCA
BsaI 712 GAGACC GGTCTC(1/5)
2032 GAGACC
BseRI 815 CTCCTC GAGGAG(10/8)
441 GAGGAG
EarI 554 GAAGAG CTCTTC(1/4)
1882 GAAGAG
FbaI 1464 TGATCA T^GATCA
2169 TGATCA
HindIII 1543 AAGCTT A^AGCTT
2137 AAGCTT
Ksp632I 554 GAAGAG CTCTTC(1/4)
1882 GAAGAG
MaeII 762 ACGT A^CGT
1485 ACGT
NcoI 609 CCATGG C^CATGG
843 CCATGG
PpuMI 1068 GGGACCT [GA]G^G[AT]CC[CT]
1152 AGGACCT
TaqI 951 TCGA T^CGA
2159 TCGA
Tsp45I 56 GTGAC ^GT[GC]AC
941 GTCAC
TthHB8I 951 TCGA T^CGA
2159 TCGA
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# 3 OR MORE CUT ENZYMES ( 72 enzymes) #
#########################################
AciI AcsI AfaI AluI ApoI
Asp700I AspHI AvaII BbsI BbvI
BfaI BglII BmyI BpuAI BsaJI
BsiHKAI BsiYI BslI BsmAI BsmFI
Bsp1286I BsrDI BsrI BssKI BstF5I
BstNI BstYI Cac8I Cfr13I DdeI
DpnII DraII DsaI EcoO109I EcoRII
EcoT14I Fnu4HI FokI HaeIII HincII
HindII HinfI HphI ItaI MaeI
MaeIII MboI MboII MflI MnlI
MseI MslI MvaI MwoI NdeII
NlaIII NlaIV PleI RsaI Sau3AI
Sau96I ScrFI SfaNI SmlI StyI
TfiI Tru9I TseI Tsp509I TspRI
XhoII XmnI