(Circular) (Six-base) MAPSORT of: pFN21ASDA0723.seq Check: 4418 from: 1 to: 7270 pFN21ASDA0723 7270 bp With 182 enzymes: SgfI * January 16, 2009 15:30 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5080 278 Size: 53 83 186 936 3544 2468 Fragments arranged by size: 3544 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3065 4613 1937 Size: 1128 1548 4594 Fragments arranged by size: 4594 1548 1128 Acc65I G'GTAC_C Cuts at: 4591 4591 Size: 7270 AclI AA'CG_TT Cuts at: 1563 5397 5770 1563 Size: 3834 373 3063 Fragments arranged by size: 3834 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2493 3483 4005 4125 5333 6717 6985 1632 Size: 861 990 522 120 1208 1384 268 1917 Fragments arranged by size: 1917 1384 1208 990 861 522 268 120 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7073 AflIII A'CryG_T Cuts at: 1203 3508 6169 7146 1203 Size: 2305 2661 977 1327 Fragments arranged by size: 2661 2305 1327 977 AgeI A'CCGG_T Cuts at: 6101 6101 Size: 7270 AhdI GACnn_n'nnGTC Cuts at: 5999 5999 Size: 7270 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7270 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7270 AlwNI CAG_nnn'CTG Cuts at: 4256 5013 6585 4256 Size: 757 1572 4941 Fragments arranged by size: 4941 1572 757 ApaLI G'TGCA_C Cuts at: 2235 5327 6483 2235 Size: 3092 1156 3022 Fragments arranged by size: 3092 3022 1156 ApoI r'AATT_y Cuts at: 1493 2431 2756 3084 4517 4579 4805 4957 7066 Size: 938 325 328 1433 62 226 152 2109 Cuts at: 7066 1493 Size: 1697 Fragments arranged by size: 2109 1697 1433 938 328 325 226 152 62 AseI AT'TA_AT Cuts at: 160 1032 3324 5824 160 Size: 872 2292 2500 1606 Fragments arranged by size: 2500 2292 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7270 AvaI C'yCGr_G Cuts at: 1870 1943 4595 1870 Size: 73 2652 4545 Fragments arranged by size: 4545 2652 73 AvrII C'CTAG_G Cuts at: 2543 2543 Size: 7270 BamHI G'GATC_C Cuts at: 4600 4967 4600 Size: 367 6903 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4591 6046 618 Size: 325 217 627 2804 1455 1842 Fragments arranged by size: 2804 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2230 4589 1391 Size: 839 2359 4072 Fragments arranged by size: 4072 2359 839 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2235 2865 3878 928 Size: 971 336 630 1013 4320 Fragments arranged by size: 4320 1013 971 630 336 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5461 5495 1903 Size: 34 3524 34 3678 Fragments arranged by size: 3678 3524 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5162 6372 5162 Size: 1210 6060 BclI T'GATC_A Cuts at: 1548 3487 4192 1548 Size: 1939 705 4626 Fragments arranged by size: 4626 1939 705 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5881 136 Size: 107 122 71 631 605 4209 1525 Fragments arranged by size: 4209 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2510 2810 3806 7265 2510 Size: 300 996 3459 2515 Fragments arranged by size: 3459 2515 996 300 BlpI GC'TnA_GC Cuts at: 3573 4687 3573 Size: 1114 6156 Bme1580I G_kGCm'C Cuts at: 1790 2239 5331 6487 1790 Size: 449 3092 1156 2573 Fragments arranged by size: 3092 2573 1156 449 BmgBI CAC'GTC Cuts at: 1318 2786 3509 1318 Size: 1468 723 5079 Fragments arranged by size: 5079 1468 723 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2116 2609 5001 Size: 416 53 83 399 24 699 493 2392 Cuts at: 5001 5959 442 Size: 958 1753 Fragments arranged by size: 2392 1753 958 699 493 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7270 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2321 2853 2321 Size: 532 6738 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3801 5930 1239 Size: 2562 2129 2579 Fragments arranged by size: 2579 2562 2129 Bpu10I CC'TnA_GC Cuts at: 3363 3363 Size: 7270 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2509 2631 3274 3548 5395 6260 6558 2509 Size: 122 643 274 1847 865 298 3221 Fragments arranged by size: 3221 1847 865 643 298 274 122 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2729 4049 5933 882 Size: 617 1230 1320 1884 2219 Fragments arranged by size: 2219 1884 1320 1230 617 BsaAI yAC'GTr Cuts at: 493 1204 4567 5061 493 Size: 711 3363 494 2702 Fragments arranged by size: 3363 2702 711 494 BsaBI GATnn'nnATC Cuts at: 7264 7264 Size: 7270 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 5077 5459 6094 275 Size: 53 83 186 936 3544 382 635 1451 Fragments arranged by size: 3544 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3914 5703 6101 6375 6522 6933 3914 Size: 1789 398 274 147 411 4251 Fragments arranged by size: 4251 1789 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 7045 Fragments arranged by size: 7045 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2479 2829 1186 Size: 1293 350 5627 Fragments arranged by size: 5627 1293 350 BseYI C'CCAG_C Cuts at: 6473 6969 6473 Size: 496 6774 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2154 1163 Size: 573 418 6279 Fragments arranged by size: 6279 573 418 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4720 5481 6509 1540 Size: 275 186 2719 761 1028 2301 Fragments arranged by size: 2719 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2230 2239 2903 5331 5416 6487 2230 Size: 9 664 2428 85 1071 3013 Fragments arranged by size: 3013 2428 1071 664 85 9 BsmI GAATG_Cn' Cuts at: 3535 4781 4874 3535 Size: 1246 93 5931 Fragments arranged by size: 5931 1246 93 BsmBI CGTCTCn'nnnn_ Cuts at: 4044 4044 Size: 7270 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2230 2239 2903 4589 5331 5416 6487 Size: 399 440 9 664 1686 742 85 1071 Cuts at: 6487 1391 Size: 2174 Fragments arranged by size: 2174 1686 1071 742 664 440 399 85 9 BspEI T'CCGG_A Cuts at: 6933 6933 Size: 7270 BspHI T'CATG_A Cuts at: 5159 6889 5159 Size: 1730 5540 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3177 4625 844 Size: 2333 1448 3489 Fragments arranged by size: 3489 2333 1448 BsrBI CCG'CTC Cuts at: 1394 1823 5157 1394 Size: 429 3334 3507 Fragments arranged by size: 3507 3334 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3663 3669 4480 5765 5939 65 Size: 1183 2415 6 811 1285 174 1396 Fragments arranged by size: 2415 1396 1285 1183 811 174 6 BsrFI r'CCGG_y Cuts at: 1700 1810 5914 6101 1700 Size: 110 4104 187 2869 Fragments arranged by size: 4104 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7270 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7270 BssSI C'ACGA_G Cuts at: 5330 6342 5330 Size: 1012 6258 BstAPI GCAn_nnn'nTGC Cuts at: 794 2177 794 Size: 1383 5887 BstBI TT'CG_AA Cuts at: 2007 6074 2007 Size: 4067 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7270 BstYI r'GATC_y Cuts at: 1972 2510 2810 3806 4600 4967 5352 5369 6821 Size: 538 300 996 794 367 385 17 1452 Cuts at: 6821 6907 6919 7265 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 996 794 538 385 367 346 300 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2326 2632 2866 513 Size: 552 1261 306 234 4917 Fragments arranged by size: 4917 1261 552 306 234 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6531 Fragments arranged by size: 6531 683 56 BtsI GCAGTG_nn' Cuts at: 3378 4022 4786 5580 5600 3378 Size: 644 764 794 20 5048 Fragments arranged by size: 5048 794 764 644 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7270 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7235 DraI TTT'AAA Cuts at: 4574 4574 Size: 7270 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6277 809 Size: 507 4961 1802 Fragments arranged by size: 4961 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2358 4717 5606 Size: 54 1425 50 275 18 528 2359 889 Cuts at: 5606 7235 8 Size: 1629 43 Fragments arranged by size: 2359 1629 1425 889 528 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4717 1537 Size: 275 2905 4090 Fragments arranged by size: 4090 2905 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2572 3900 5200 1353 Size: 1219 1328 1300 3423 Fragments arranged by size: 3423 1328 1300 1219 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5857 6231 6377 387 Size: 1064 138 4268 374 146 1280 Fragments arranged by size: 4268 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2228 2228 Size: 7270 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2493 3483 3801 4005 4125 5333 5930 Size: 393 861 990 318 204 120 1208 597 Cuts at: 5930 6717 6985 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1208 990 861 787 597 393 318 268 204 120 EcoNI CCTnn'n_nnAGG Cuts at: 4416 4416 Size: 7270 EcoO109I rG'GnC_Cy Cuts at: 2480 3092 3176 2480 Size: 612 84 6574 Fragments arranged by size: 6574 612 84 EcoRI G'AATT_C Cuts at: 3084 4579 4957 3084 Size: 1495 378 5397 Fragments arranged by size: 5397 1495 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3642 3642 Size: 7270 FspI TGC'GCA Cuts at: 5776 5776 Size: 7270 HaeII r_GCGC'y Cuts at: 1678 2838 6417 1678 Size: 1160 3579 2531 Fragments arranged by size: 3579 2531 1160 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2389 2421 2495 2527 3604 Size: 32 135 32 817 32 74 32 1077 Cuts at: 3604 3636 3833 3865 5911 5943 5985 6017 1373 Size: 32 197 32 2046 32 42 32 2626 Fragments arranged by size: 2626 2046 1077 817 197 135 74 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2945 3670 3721 4614 4860 669 Size: 988 281 1007 725 51 893 246 3079 Fragments arranged by size: 3079 1007 988 893 725 281 246 51 HindIII A'AGCT_T Cuts at: 748 3566 4160 748 Size: 2818 594 3858 Fragments arranged by size: 3858 2818 594 HpaI GTT'AAC Cuts at: 4860 4860 Size: 7270 KpnI G_GTAC'C Cuts at: 4595 4595 Size: 7270 MfeI C'AATT_G Cuts at: 4869 4869 Size: 7270 MluI A'CGCG_T Cuts at: 7146 7146 Size: 7270 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3112 4194 6384 6568 1305 Size: 1807 1082 2190 184 2007 Fragments arranged by size: 2190 2007 1807 1082 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2360 10 Size: 54 1425 343 528 4920 Fragments arranged by size: 4920 1425 528 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2076 2952 3605 5228 5587 5746 518 Size: 648 910 876 653 1623 359 159 2042 Fragments arranged by size: 2042 1623 910 876 653 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3274 4686 5363 6511 6756 1606 Size: 1668 1412 677 1148 245 2120 Fragments arranged by size: 2120 1668 1412 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2632 2866 513 Size: 552 1567 234 4917 Fragments arranged by size: 4917 1567 552 234 NdeI CA'TA_TG Cuts at: 387 387 Size: 7270 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7270 NotI GC'GGCC_GC Cuts at: 4717 4717 Size: 7270 NspI r_CATG'y Cuts at: 1132 1229 3190 4628 6173 1132 Size: 97 1961 1438 1545 2229 Fragments arranged by size: 2229 1961 1545 1438 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7270 PciI A'CATG_T Cuts at: 6169 6169 Size: 7270 PflMI CCAn_nnn'nTGG Cuts at: 1108 3131 1108 Size: 2023 5247 PmeI GTTT'AAAC Cuts at: 4574 4574 Size: 7270 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5339 5371 6852 6884 5339 Size: 32 1481 32 5725 Fragments arranged by size: 5725 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3092 3176 3092 Size: 84 7186 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7270 PsiI TTA'TAA Cuts at: 4840 4840 Size: 7270 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 4423 4455 4423 Size: 32 7238 PstI C_TGCA'G Cuts at: 830 4622 830 Size: 3792 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7270 PvuII CAG'CTG Cuts at: 3274 3274 Size: 7270 SacI G_AGCT'C Cuts at: 2230 2230 Size: 7270 SalI G'TCGA_C Cuts at: 1936 4612 1936 Size: 2676 4594 SbfI CC_TGCA'GG Cuts at: 4622 4622 Size: 7270 ScaI AGT'ACT Cuts at: 2254 5518 7230 2254 Size: 3264 1712 2294 Fragments arranged by size: 3264 2294 1712 SexAI A'CCwGG_T Cuts at: 4249 4249 Size: 7270 SfcI C'TryA_G Cuts at: 826 1045 2450 4618 5753 6434 6625 826 Size: 219 1405 2168 1135 681 191 1471 Fragments arranged by size: 2168 1471 1405 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7270 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7270 SmaI CCC'GGG Cuts at: 1872 4597 1872 Size: 2725 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2488 2646 3253 3563 5374 6275 Size: 197 926 545 158 607 310 1811 901 Cuts at: 6275 6537 820 Size: 262 1553 Fragments arranged by size: 1811 1553 926 901 607 545 310 262 197 158 SnaBI TAC'GTA Cuts at: 493 4567 5061 493 Size: 4074 494 2702 Fragments arranged by size: 4074 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 7270 SphI G_CATG'C Cuts at: 1132 4628 1132 Size: 3496 3774 SspI AAT'ATT Cuts at: 5 52 2854 5194 5 Size: 47 2802 2340 2081 Fragments arranged by size: 2802 2340 2081 47 StuI AGG'CCT Cuts at: 1517 2103 1517 Size: 586 6684 StyI C'CwwG_G Cuts at: 513 1065 2543 2632 2866 4709 513 Size: 552 1478 89 234 1843 3074 Fragments arranged by size: 3074 1843 1478 552 234 89 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3029 3140 4073 5297 5314 5467 5652 Size: 1255 1449 111 933 1224 17 153 185 Cuts at: 5652 6097 325 Size: 445 1498 Fragments arranged by size: 1498 1449 1255 1224 933 445 185 153 111 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2252 5516 7228 Size: 274 80 33 51 1443 275 3264 1712 Cuts at: 7228 96 Size: 138 Fragments arranged by size: 3264 1712 1443 275 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 7230 7262 7230 Size: 32 7238 Tth111I GACn'n_nGTC Cuts at: 1316 2787 1316 Size: 1471 5799 XbaI T'CTAG_A Cuts at: 4606 4606 Size: 7270 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2353 2353 Size: 7270 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7270 XmaI C'CCGG_G Cuts at: 1870 4595 1870 Size: 2725 4545 XmnI GAAnn'nnTTC Cuts at: 2203 2641 4138 5399 2203 Size: 438 1497 1261 4074 Fragments arranged by size: 4074 1497 1261 438 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5078 276 Size: 53 83 186 936 3544 2468 Fragments arranged by size: 3544 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PsrI PstI PvuI PvuII SacI SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI BaeI BbeI BbvCI BfrBI BsiWI BstXI BstZ17I Bsu36I DraIII EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI RsrII SacII SanDI SapI SfiI SfoI SrfI SwaI