Restriction map for
pFN21ASDA0723
(Linear) (Six-base) MAPSORT of: KIAA0723.seq Check: 2191 from: 1 to: 2541
KIAA0723.orf
With 182 enzymes: SgfI *
October 24, 2006 15:13 ..
AccI GT'mk_AC
Cuts at: 0 1042 2541
Size: 1042 1499
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 470 1460 1982 2102 2541
Size: 470 990 522 120 439
Fragments arranged by size:
990 522 470 439 120
AflIII A'CryG_T
Cuts at: 0 1485 2541
Size: 1485 1056
AlwNI CAG_nnn'CTG
Cuts at: 0 2233 2541
Size: 2233 308
ApaLI G'TGCA_C
Cuts at: 0 212 2541
Size: 212 2329
ApoI r'AATT_y
Cuts at: 0 408 733 1061 2494 2541
Size: 408 325 328 1433 47
Fragments arranged by size:
1433 408 328 325 47
AseI AT'TA_AT
Cuts at: 0 1301 2541
Size: 1301 1240
AvrII C'CTAG_G
Cuts at: 0 520 2541
Size: 520 2021
BanII G_rGCy'C
Cuts at: 0 207 2541
Size: 207 2334
BbsI GAAGACnn'nnnn_
Cuts at: 0 212 842 1855 2541
Size: 212 630 1013 686
Fragments arranged by size:
1013 686 630 212
BclI T'GATC_A
Cuts at: 0 1464 2169 2541
Size: 1464 705 372
Fragments arranged by size:
1464 705 372
BglII A'GATC_T
Cuts at: 0 487 787 1783 2541
Size: 487 300 996 758
Fragments arranged by size:
996 758 487 300
BlpI GC'TnA_GC
Cuts at: 0 1550 2541
Size: 1550 991
Bme1580I G_kGCm'C
Cuts at: 0 216 2541
Size: 216 2325
BmgBI CAC'GTC
Cuts at: 0 763 1486 2541
Size: 763 723 1055
Fragments arranged by size:
1055 763 723
BmrI ACTGGGnnnn_n'
Cuts at: 0 93 586 2541
Size: 93 493 1955
Fragments arranged by size:
1955 493 93
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 298 830 2541
Size: 298 532 1711
Fragments arranged by size:
1711 532 298
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1778 2541
Size: 1778 763
Bpu10I CC'TnA_GC
Cuts at: 0 1340 2541
Size: 1340 1201
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 486 608 1251 1525 2541
Size: 486 122 643 274 1016
Fragments arranged by size:
1016 643 486 274 122
BsaI GGTCTCn'nnnn_
Cuts at: 0 706 2026 2541
Size: 706 1320 515
Fragments arranged by size:
1320 706 515
BsaWI w'CCGG_w
Cuts at: 0 1891 2541
Size: 1891 650
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 456 806 2541
Size: 456 350 1735
Fragments arranged by size:
1735 456 350
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 131 2541
Size: 131 2410
BsiHKAI G_wGCw'C
Cuts at: 0 207 216 880 2541
Size: 207 9 664 1661
Fragments arranged by size:
1661 664 207 9
BsmI GAATG_Cn'
Cuts at: 0 1512 2541
Size: 1512 1029
BsmBI CGTCTCn'nnnn_
Cuts at: 0 2021 2541
Size: 2021 520
Bsp1286I G_dGCh'C
Cuts at: 0 207 216 880 2541
Size: 207 9 664 1661
Fragments arranged by size:
1661 664 207 9
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1154 2541
Size: 1154 1387
BsrDI GCAATG_nn'
Cuts at: 0 1640 1646 2457 2541
Size: 1640 6 811 84
Fragments arranged by size:
1640 811 84 6
BstAPI GCAn_nnn'nTGC
Cuts at: 0 154 2541
Size: 154 2387
BstYI r'GATC_y
Cuts at: 0 487 787 1783 2541
Size: 487 300 996 758
Fragments arranged by size:
996 758 487 300
BtgI C'CryG_G
Cuts at: 0 303 609 843 2541
Size: 303 306 234 1698
Fragments arranged by size:
1698 306 303 234
BtsI GCAGTG_nn'
Cuts at: 0 1355 1999 2541
Size: 1355 644 542
Fragments arranged by size:
1355 644 542
EaeI y'GGCC_r
Cuts at: 0 335 2541
Size: 335 2206
EarI CTCTTCn'nnn_
Cuts at: 0 549 1877 2541
Size: 549 1328 664
Fragments arranged by size:
1328 664 549
EcoICRI GAG'CTC
Cuts at: 0 205 2541
Size: 205 2336
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 470 1460 1778 1982 2102 2541
Size: 470 990 318 204 120 439
Fragments arranged by size:
990 470 439 318 204 120
EcoNI CCTnn'n_nnAGG
Cuts at: 0 2393 2541
Size: 2393 148
EcoO109I rG'GnC_Cy
Cuts at: 0 457 1069 1153 2541
Size: 457 612 84 1388
Fragments arranged by size:
1388 612 457 84
EcoRI G'AATT_C
Cuts at: 0 1061 2541
Size: 1061 1480
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1619 2541
Size: 1619 922
HaeII r_GCGC'y
Cuts at: 0 815 2541
Size: 815 1726
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 366 398 472 504 1581 1613 1810 1842
Size: 366 32 74 32 1077 32 197 32
Cuts at: 1842 2541
Size: 699
Fragments arranged by size:
1077 699 366 197 74 32 32 32
32
HincII GTy'rAC
Cuts at: 0 922 1647 1698 2541
Size: 922 725 51 843
Fragments arranged by size:
922 843 725 51
HindIII A'AGCT_T
Cuts at: 0 1543 2137 2541
Size: 1543 594 404
Fragments arranged by size:
1543 594 404
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1089 2171 2541
Size: 1089 1082 370
Fragments arranged by size:
1089 1082 370
MscI TGG'CCA
Cuts at: 0 337 2541
Size: 337 2204
MslI CAynn'nnrTG
Cuts at: 0 53 929 1582 2541
Size: 53 876 653 959
Fragments arranged by size:
959 876 653 53
MspA1I CmG'CkG
Cuts at: 0 1251 2541
Size: 1251 1290
NcoI C'CATG_G
Cuts at: 0 609 843 2541
Size: 609 234 1698
Fragments arranged by size:
1698 609 234
NspI r_CATG'y
Cuts at: 0 1167 2541
Size: 1167 1374
PflMI CCAn_nnn'nTGG
Cuts at: 0 1108 2541
Size: 1108 1433
PpuMI rG'GwC_Cy
Cuts at: 0 1069 1153 2541
Size: 1069 84 1388
Fragments arranged by size:
1388 1069 84
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 2400 2432 2541
Size: 2400 32 109
Fragments arranged by size:
2400 109 32
PvuII CAG'CTG
Cuts at: 0 1251 2541
Size: 1251 1290
SacI G_AGCT'C
Cuts at: 0 207 2541
Size: 207 2334
ScaI AGT'ACT
Cuts at: 0 231 2541
Size: 231 2310
SexAI A'CCwGG_T
Cuts at: 0 2226 2541
Size: 2226 315
SfcI C'TryA_G
Cuts at: 0 427 2541
Size: 427 2114
SmlI C'TyrA_G
Cuts at: 0 465 623 1230 1540 2541
Size: 465 158 607 310 1001
Fragments arranged by size:
1001 607 465 310 158
SspI AAT'ATT
Cuts at: 0 831 2541
Size: 831 1710
StuI AGG'CCT
Cuts at: 0 80 2541
Size: 80 2461
StyI C'CwwG_G
Cuts at: 0 520 609 843 2541
Size: 520 89 234 1698
Fragments arranged by size:
1698 520 234 89
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1006 1117 2050 2541
Size: 1006 111 933 491
Fragments arranged by size:
1006 933 491 111
TatI w'GTAC_w
Cuts at: 0 229 2541
Size: 229 2312
Tth111I GACn'n_nGTC
Cuts at: 0 764 2541
Size: 764 1777
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 330 2541
Size: 330 2211
XmnI GAAnn'nnTTC
Cuts at: 0 180 618 2115 2541
Size: 180 438 1497 426
Fragments arranged by size:
1497 438 426 180
Enzymes that do cut:
AccI AcuI AflIII AlwNI ApaLI ApoI AseI AvrII
BanII BbsI BclI BglII BlpI Bme1580I BmgBI BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaWI BseRI BsgI
BsiHKAI BsmI BsmBI Bsp1286I BspMI BsrDI BstAPI BstYI
BtgI BtsI EaeI EarI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI HaeII Hin4I HincII HindIII MmeI MscI
MslI MspA1I NcoI NspI PflMI PpuMI PsrI PvuII
SacI ScaI SexAI SfcI SmlI SspI StuI StyI
TaqII TatI Tth111I XcmI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AgeI AhdI
AleI AlfI AloI ApaI AscI AsiSI AvaI BaeI
BamHI BanI BbeI BbvCI BcgI BciVI BfrBI BglI
BmtI BsaAI BsaBI BsaHI BsaXI BseYI BsiEI BsiWI
BspEI BspHI BsrBI BsrFI BsrGI BssHII BssSI BstBI
BstEII BstXI BstZ17I Bsu36I BtgZI ClaI CspCI DraI
DraIII DrdI EagI EciI EcoRV FseI FspI FspAI
HpaI KasI KpnI MfeI MluI NaeI NarI NdeI
NgoMIV NheI NotI NruI NsiI PacI PasI PciI
PfoI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI
PstI PvuI RsrII SacII SalI SanDI SapI SbfI
SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI
SrfI SwaI TstI XbaI XhoI XmaI ZraI