(Circular) (Six-base) MAPSORT of: pFN21ASDA0722.seq Check: 5362 from: 1 to: 7879 pFN21ASDA0722 7879 bp With 182 enzymes: SgfI * January 16, 2009 15:30 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3285 3322 4813 3285 Size: 37 1491 6351 Fragments arranged by size: 6351 1491 37 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5689 278 Size: 53 83 186 936 4153 2468 Fragments arranged by size: 4153 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2286 2655 5222 1937 Size: 349 369 2567 4594 Fragments arranged by size: 4594 2567 369 349 Acc65I G'GTAC_C Cuts at: 2532 5200 2532 Size: 2668 5211 AclI AA'CG_TT Cuts at: 1563 6006 6379 1563 Size: 4443 373 3063 Fragments arranged by size: 4443 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2243 4631 4754 4948 5942 7326 7594 1632 Size: 611 2388 123 194 994 1384 268 1917 Fragments arranged by size: 2388 1917 1384 994 611 268 194 123 AfeI AGC'GCT Cuts at: 4898 4898 Size: 7879 AflII C'TTAA_G Cuts at: 820 1017 4130 820 Size: 197 3113 4569 Fragments arranged by size: 4569 3113 197 AflIII A'CryG_T Cuts at: 1203 6778 7755 1203 Size: 5575 977 1327 Fragments arranged by size: 5575 1327 977 AgeI A'CCGG_T Cuts at: 6710 6710 Size: 7879 AhdI GACnn_n'nnGTC Cuts at: 6608 6608 Size: 7879 AleI CACnn'nnGTG Cuts at: 1166 3332 1166 Size: 2166 5713 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 5010 1184 Size: 3826 4053 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4994 5026 4994 Size: 32 7847 AlwNI CAG_nnn'CTG Cuts at: 2197 2665 2707 2992 3946 4421 4879 5622 7194 Size: 468 42 285 954 475 458 743 1572 Cuts at: 7194 2197 Size: 2882 Fragments arranged by size: 2882 1572 954 743 475 468 458 285 42 ApaI G_GGCC'C Cuts at: 4052 4085 4328 4355 4401 4643 4052 Size: 33 243 27 46 242 7288 Fragments arranged by size: 7288 243 242 46 33 27 ApaLI G'TGCA_C Cuts at: 5936 7092 5936 Size: 1156 6723 ApoI r'AATT_y Cuts at: 1493 5188 5414 5566 7675 1493 Size: 3695 226 152 2109 1697 Fragments arranged by size: 3695 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 6433 160 Size: 872 5401 1606 Fragments arranged by size: 5401 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7879 AvaI C'yCGr_G Cuts at: 1870 1943 2591 2754 2914 3045 3644 4162 4300 Size: 73 648 163 160 131 599 518 138 Cuts at: 4300 5204 1870 Size: 904 4545 Fragments arranged by size: 4545 904 648 599 518 163 160 138 131 73 BamHI G'GATC_C Cuts at: 5209 5576 5209 Size: 367 7512 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2096 2393 2532 2645 2677 Size: 325 217 627 309 297 139 113 32 Cuts at: 2677 3136 3302 4256 4390 5200 6655 618 Size: 459 166 954 134 810 1455 1842 Fragments arranged by size: 1842 1455 954 810 627 459 325 309 297 217 166 139 134 113 32 BanII G_rGCy'C Cuts at: 1391 2031 3185 3836 3890 4052 4085 4246 4295 Size: 640 1154 651 54 162 33 161 49 Cuts at: 4295 4328 4355 4401 4428 4643 5198 1391 Size: 33 27 46 27 215 555 4072 Fragments arranged by size: 4072 1154 651 640 555 215 162 161 54 49 46 33 33 27 27 BbeI G_GCGC'C Cuts at: 2100 2397 2681 3140 3306 2100 Size: 297 284 459 166 6673 Fragments arranged by size: 6673 459 297 284 166 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2104 3005 3932 4273 928 Size: 971 205 901 927 341 4534 Fragments arranged by size: 4534 971 927 901 341 205 BbvCI CC'TCA_GC Cuts at: 3132 4210 3132 Size: 1078 6801 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2809 2843 4448 4482 6070 6104 1903 Size: 34 872 34 1605 34 1588 34 3678 Fragments arranged by size: 3678 1605 1588 872 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5771 6981 5771 Size: 1210 6669 BclI T'GATC_A Cuts at: 1548 1548 Size: 7879 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2040 3014 3276 Size: 107 122 71 631 605 368 974 262 Cuts at: 3276 3435 4089 4359 4628 6490 136 Size: 159 654 270 269 1862 1525 Fragments arranged by size: 1862 1525 974 654 631 605 368 270 269 262 159 122 107 71 BglII A'GATC_T Cuts at: 7874 7874 Size: 7879 BlpI GC'TnA_GC Cuts at: 2351 3474 4691 5296 2351 Size: 1123 1217 605 4934 Fragments arranged by size: 4934 1217 1123 605 Bme1580I G_kGCm'C Cuts at: 1790 2901 3909 4052 4085 4259 4328 4355 4401 Size: 1111 1008 143 33 174 69 27 46 Cuts at: 4401 4643 5940 7096 1790 Size: 242 1297 1156 2573 Fragments arranged by size: 2573 1297 1156 1111 1008 242 174 143 69 46 33 27 BmgBI CAC'GTC Cuts at: 1318 3715 4591 1318 Size: 2397 876 4606 Fragments arranged by size: 4606 2397 876 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3135 3252 3314 Size: 416 53 83 399 24 1718 117 62 Cuts at: 3314 3373 5610 6568 442 Size: 59 2237 958 1753 Fragments arranged by size: 2237 1753 1718 958 416 399 117 83 62 59 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7879 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2522 2917 3271 3592 4419 4499 4616 5084 Size: 1283 395 354 321 827 80 117 468 Cuts at: 5084 6539 1239 Size: 1455 2579 Fragments arranged by size: 2579 1455 1283 827 468 395 354 321 117 80 Bpu10I CC'TnA_GC Cuts at: 3132 3558 4210 4422 4880 3132 Size: 426 652 212 458 6131 Fragments arranged by size: 6131 652 458 426 212 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3525 6004 6869 7167 3525 Size: 2479 865 298 4237 Fragments arranged by size: 4237 2479 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2042 2753 4514 6542 882 Size: 617 543 711 1761 2028 2219 Fragments arranged by size: 2219 2028 1761 711 617 543 BsaAI yAC'GTr Cuts at: 493 1204 5176 5670 493 Size: 711 3972 494 2702 Fragments arranged by size: 3972 2702 711 494 BsaBI GATnn'nnATC Cuts at: 7873 7873 Size: 7879 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2097 2394 2678 3137 Size: 53 83 186 936 564 297 284 459 Cuts at: 3137 3303 3486 3581 3983 5686 6068 6703 275 Size: 166 183 95 402 1703 382 635 1451 Fragments arranged by size: 1703 1451 936 635 564 459 402 382 297 284 186 183 166 95 83 53 BsaWI w'CCGG_w Cuts at: 3996 6312 6710 6984 7131 7542 3996 Size: 2316 398 274 147 411 4333 Fragments arranged by size: 4333 2316 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2945 2975 3214 3244 3520 Size: 30 165 30 1542 30 239 30 276 Cuts at: 3520 3550 1178 Size: 30 5507 Fragments arranged by size: 5507 1542 276 239 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3528 3625 4532 1186 Size: 2342 97 907 4533 Fragments arranged by size: 4533 2342 907 97 BseYI C'CCAG_C Cuts at: 2200 2607 3670 3792 3932 4250 4414 7082 7578 Size: 407 1063 122 140 318 164 2668 496 Cuts at: 7578 2200 Size: 2501 Fragments arranged by size: 2668 2501 1063 496 407 318 164 140 122 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2318 2393 3020 3193 3356 3624 3668 Size: 573 582 75 627 173 163 268 44 Cuts at: 3668 4217 4604 4997 5006 1163 Size: 549 387 393 9 4036 Fragments arranged by size: 4036 627 582 573 549 393 387 268 173 163 75 44 9 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2035 2476 3220 5329 6090 7118 Size: 275 186 34 441 744 2109 761 1028 Cuts at: 7118 1540 Size: 2301 Fragments arranged by size: 2301 2109 1028 761 744 441 275 186 34 BsiHKAI G_wGCw'C Cuts at: 3185 3651 4313 4428 4542 5940 6025 7096 3185 Size: 466 662 115 114 1398 85 1071 3968 Fragments arranged by size: 3968 1398 1071 662 466 115 114 85 BsmI GAATG_Cn' Cuts at: 4932 5390 5483 4932 Size: 458 93 7328 Fragments arranged by size: 7328 458 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2031 2901 3185 3651 3836 3890 3909 Size: 399 241 870 284 466 185 54 19 Cuts at: 3909 4052 4085 4246 4259 4295 4313 4328 4355 Size: 143 33 161 13 36 18 15 27 Cuts at: 4355 4401 4428 4542 4643 5198 5940 6025 7096 Size: 46 27 114 101 555 742 85 1071 Cuts at: 7096 1391 Size: 2174 Fragments arranged by size: 2174 1071 870 742 555 466 399 284 241 185 161 143 114 101 85 54 46 36 33 27 27 19 18 15 13 BspEI T'CCGG_A Cuts at: 7542 7542 Size: 7879 BspHI T'CATG_A Cuts at: 5768 7498 5768 Size: 1730 6149 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2340 2715 3285 3322 3372 3402 3606 3834 Size: 1496 375 570 37 50 30 204 228 Cuts at: 3834 3954 4131 4813 5234 844 Size: 120 177 682 421 3489 Fragments arranged by size: 3489 1496 682 570 421 375 228 204 177 120 50 37 30 BsrBI CCG'CTC Cuts at: 1394 1823 3562 3576 4305 5137 5766 1394 Size: 429 1739 14 729 832 629 3507 Fragments arranged by size: 3507 1739 832 729 629 429 14 BsrDI GCAATG_nn' Cuts at: 65 1248 4464 6374 6548 65 Size: 1183 3216 1910 174 1396 Fragments arranged by size: 3216 1910 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2471 3519 4092 4790 6523 6710 1700 Size: 110 661 1048 573 698 1733 187 2869 Fragments arranged by size: 2869 1733 1048 698 661 573 187 110 BsrGI T'GTAC_A Cuts at: 96 2580 4848 96 Size: 2484 2268 3127 Fragments arranged by size: 3127 2484 2268 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7879 BssSI C'ACGA_G Cuts at: 5939 6951 5939 Size: 1012 6867 BstAPI GCAn_nnn'nTGC Cuts at: 794 2341 2992 3297 4800 4828 4984 5080 5127 Size: 1547 651 305 1503 28 156 96 47 Cuts at: 5127 794 Size: 3546 Fragments arranged by size: 3546 1547 1503 651 305 156 96 47 28 BstBI TT'CG_AA Cuts at: 2007 6683 2007 Size: 4676 3203 BstEII G'GTnAC_C Cuts at: 1184 3119 1184 Size: 1935 5944 BstXI CCAn_nnnn'nTGG Cuts at: 2584 3433 2584 Size: 849 7030 BstYI r'GATC_y Cuts at: 1972 4548 4782 5209 5576 5961 5978 7430 7516 Size: 2576 234 427 367 385 17 1452 86 Cuts at: 7516 7528 7874 1972 Size: 12 346 1977 Fragments arranged by size: 2576 1977 1452 427 385 367 346 234 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3265 4515 3265 Size: 1250 6629 BtgI C'CryG_G Cuts at: 513 1065 2022 2035 2052 2092 3214 3744 3802 Size: 552 957 13 17 40 1122 530 58 Cuts at: 3802 4033 4194 4287 513 Size: 231 161 93 4105 Fragments arranged by size: 4105 1122 957 552 530 231 161 93 58 40 17 13 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 7140 Fragments arranged by size: 7140 683 56 BtsI GCAGTG_nn' Cuts at: 3181 4944 5395 6189 6209 3181 Size: 1763 451 794 20 4851 Fragments arranged by size: 4851 1763 794 451 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7879 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7844 DraI TTT'AAA Cuts at: 5183 5183 Size: 7879 DraIII CAC_nnn'GTG Cuts at: 4390 4975 5118 4390 Size: 585 143 7151 Fragments arranged by size: 7151 585 143 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3092 3713 6886 809 Size: 507 1776 621 3173 1802 Fragments arranged by size: 3173 1802 1776 621 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2032 2089 2322 Size: 54 1425 50 275 18 202 57 233 Cuts at: 2322 2473 2556 3217 3492 3563 4061 4090 4671 Size: 151 83 661 275 71 498 29 581 Cuts at: 4671 4740 5326 6215 7844 8 Size: 69 586 889 1629 43 Fragments arranged by size: 1629 1425 889 661 586 581 498 275 275 233 202 151 83 71 69 57 54 50 43 29 18 EagI C'GGCC_G Cuts at: 1537 1812 2032 2473 3217 5326 1537 Size: 275 220 441 744 2109 4090 Fragments arranged by size: 4090 2109 744 441 275 220 EarI CTCTTCn'nnn_ Cuts at: 1353 2378 3076 4378 5809 1353 Size: 1025 698 1302 1431 3423 Fragments arranged by size: 3423 1431 1302 1025 698 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2642 3710 6466 6840 6986 387 Size: 1064 138 1053 1068 2756 374 146 1280 Fragments arranged by size: 2756 1280 1068 1064 1053 374 146 138 EcoICRI GAG'CTC Cuts at: 3183 4426 3183 Size: 1243 6636 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2243 2522 2917 3271 3592 4419 4499 Size: 393 611 279 395 354 321 827 80 Cuts at: 4499 4616 4631 4754 4948 5084 5942 6539 7326 Size: 117 15 123 194 136 858 597 787 Cuts at: 7326 7594 1239 Size: 268 1524 Fragments arranged by size: 1524 858 827 787 611 597 395 393 354 321 279 268 194 136 123 117 80 15 EcoNI CCTnn'n_nnAGG Cuts at: 2872 3627 4016 4434 4514 2872 Size: 755 389 418 80 6237 Fragments arranged by size: 6237 755 418 389 80 EcoO109I rG'GnC_Cy Cuts at: 2080 2317 2704 3618 4025 4049 4072 4081 4082 Size: 237 387 914 407 24 23 9 1 Cuts at: 4082 4324 4325 4352 4398 4411 4501 4639 4640 Size: 242 1 27 46 13 90 138 1 Cuts at: 4640 4876 5050 2080 Size: 236 174 4909 Fragments arranged by size: 4909 914 407 387 242 237 236 174 138 90 46 27 24 23 13 9 1 1 1 EcoRI G'AATT_C Cuts at: 5188 5566 5188 Size: 378 7501 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2698 4102 2698 Size: 1404 6475 FseI GG_CCGG'CC Cuts at: 3523 3523 Size: 7879 FspI TGC'GCA Cuts at: 2345 4832 6385 2345 Size: 2487 1553 3839 Fragments arranged by size: 3839 2487 1553 FspAI rTGC'GCAy Cuts at: 2345 2345 Size: 7879 HaeII r_GCGC'y Cuts at: 1678 2100 2397 2681 3140 3306 3693 4623 4900 Size: 422 297 284 459 166 387 930 277 Cuts at: 4900 5153 7026 1678 Size: 253 1873 2531 Fragments arranged by size: 2531 1873 930 459 422 387 297 284 277 253 166 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3214 3246 3973 4005 4736 Size: 32 135 32 1642 32 727 32 731 Cuts at: 4736 4768 6520 6552 6594 6626 1373 Size: 32 1752 32 42 32 2626 Fragments arranged by size: 2626 1752 1642 731 727 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 5223 5469 669 Size: 988 281 3285 246 3079 Fragments arranged by size: 3285 3079 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7879 HpaI GTT'AAC Cuts at: 5469 5469 Size: 7879 KasI G'GCGC_C Cuts at: 2096 2393 2677 3136 3302 2096 Size: 297 284 459 166 6673 Fragments arranged by size: 6673 459 297 284 166 KpnI G_GTAC'C Cuts at: 2536 5204 2536 Size: 2668 5211 MfeI C'AATT_G Cuts at: 5478 5478 Size: 7879 MluI A'CGCG_T Cuts at: 7755 7755 Size: 7879 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2487 3526 6993 7177 1305 Size: 1182 1039 3467 184 2007 Fragments arranged by size: 3467 2007 1182 1039 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3494 10 Size: 54 1425 343 1662 4395 Fragments arranged by size: 4395 1662 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2582 3332 4337 5837 6196 6355 518 Size: 648 1416 750 1005 1500 359 159 2042 Fragments arranged by size: 2042 1500 1416 1005 750 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2037 2773 2782 2992 3026 4214 4349 4654 Size: 431 736 9 210 34 1188 135 305 Cuts at: 4654 4720 4918 5295 5972 7120 7365 1606 Size: 66 198 377 677 1148 245 2120 Fragments arranged by size: 2120 1188 1148 736 677 431 377 305 245 210 198 135 66 34 9 NaeI GCC'GGC Cuts at: 2473 3521 4792 2473 Size: 1048 1271 5560 Fragments arranged by size: 5560 1271 1048 NarI GG'CG_CC Cuts at: 2097 2394 2678 3137 3303 2097 Size: 297 284 459 166 6673 Fragments arranged by size: 6673 459 297 284 166 NcoI C'CATG_G Cuts at: 513 1065 2022 4033 4194 513 Size: 552 957 2011 161 4198 Fragments arranged by size: 4198 2011 957 552 161 NdeI CA'TA_TG Cuts at: 387 387 Size: 7879 NgoMIV G'CCGG_C Cuts at: 2471 3519 4790 2471 Size: 1048 1271 5560 Fragments arranged by size: 5560 1271 1048 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7879 NotI GC'GGCC_GC Cuts at: 5326 5326 Size: 7879 NspI r_CATG'y Cuts at: 1132 1229 5083 5237 6782 1132 Size: 97 3854 154 1545 2229 Fragments arranged by size: 3854 2229 1545 154 97 PasI CC'CwG_GG Cuts at: 1348 2974 3433 3754 4321 1348 Size: 1626 459 321 567 4906 Fragments arranged by size: 4906 1626 567 459 321 PciI A'CATG_T Cuts at: 6778 6778 Size: 7879 PflMI CCAn_nnn'nTGG Cuts at: 1108 2272 1108 Size: 1164 6715 PfoI T'CCnGG_A Cuts at: 2265 4435 4593 4905 4923 5061 2265 Size: 2170 158 312 18 138 5083 Fragments arranged by size: 5083 2170 312 158 138 18 PmeI GTTT'AAAC Cuts at: 5183 5183 Size: 7879 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5948 5980 7461 7493 5948 Size: 32 1481 32 6334 Fragments arranged by size: 6334 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2080 2317 2704 3618 4025 4072 2080 Size: 237 387 914 407 47 5887 Fragments arranged by size: 5887 914 407 387 237 47 PshAI GACnn'nnGTC Cuts at: 1613 2738 1613 Size: 1125 6754 PsiI TTA'TAA Cuts at: 5449 5449 Size: 7879 PspOMI G'GGCC_C Cuts at: 4048 4081 4324 4351 4397 4639 4048 Size: 33 243 27 46 242 7288 Fragments arranged by size: 7288 243 242 46 33 27 PstI C_TGCA'G Cuts at: 830 2382 2997 3248 3369 3813 3861 4185 4406 Size: 1552 615 251 121 444 48 324 221 Cuts at: 4406 4451 4659 4896 5076 5231 830 Size: 45 208 237 180 155 3478 Fragments arranged by size: 3478 1552 615 444 324 251 237 221 208 180 155 121 48 45 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7879 PvuII CAG'CTG Cuts at: 2782 2992 4214 4654 4720 2782 Size: 210 1222 440 66 5941 Fragments arranged by size: 5941 1222 440 210 66 SacI G_AGCT'C Cuts at: 3185 4428 3185 Size: 1243 6636 SacII CC_GC'GG Cuts at: 2038 2038 Size: 7879 SalI G'TCGA_C Cuts at: 1936 5221 1936 Size: 3285 4594 SanDI GG'GwC_CC Cuts at: 4025 4025 Size: 7879 SapI GCTCTTCn'nnn_ Cuts at: 2378 4378 2378 Size: 2000 5879 SbfI CC_TGCA'GG Cuts at: 4185 5231 4185 Size: 1046 6833 ScaI AGT'ACT Cuts at: 2303 6127 7839 2303 Size: 3824 1712 2343 Fragments arranged by size: 3824 2343 1712 SexAI A'CCwGG_T Cuts at: 2289 3123 4386 2289 Size: 834 1263 5782 Fragments arranged by size: 5782 1263 834 SfcI C'TryA_G Cuts at: 826 1045 2378 2993 3244 3365 3809 3857 4181 Size: 219 1333 615 251 121 444 48 324 Cuts at: 4181 4402 4447 4655 4892 5072 5227 6362 7043 Size: 221 45 208 237 180 155 1135 681 Cuts at: 7043 7234 826 Size: 191 1471 Fragments arranged by size: 1471 1333 1135 681 615 444 324 251 237 221 219 208 191 180 155 121 48 45 SfiI GGCCn_nnn'nGGCC Cuts at: 3276 4089 3276 Size: 813 7066 SfoI GGC'GCC Cuts at: 2098 2395 2679 3138 3304 2098 Size: 297 284 459 166 6673 Fragments arranged by size: 6673 459 297 284 166 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7879 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7879 SmaI CCC'GGG Cuts at: 1872 2756 3047 4164 5206 1872 Size: 884 291 1117 1042 4545 Fragments arranged by size: 4545 1117 1042 884 291 SmlI C'TyrA_G Cuts at: 820 1017 1943 3540 4130 5983 6884 7146 820 Size: 197 926 1597 590 1853 901 262 1553 Fragments arranged by size: 1853 1597 1553 926 901 590 262 197 SnaBI TAC'GTA Cuts at: 493 5176 5670 493 Size: 4683 494 2702 Fragments arranged by size: 4683 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 7879 SphI G_CATG'C Cuts at: 1132 5237 1132 Size: 4105 3774 SspI AAT'ATT Cuts at: 5 52 5803 5 Size: 47 5751 2081 Fragments arranged by size: 5751 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7879 StyI C'CwwG_G Cuts at: 513 1065 2022 3897 4033 4077 4194 5318 513 Size: 552 957 1875 136 44 117 1124 3074 Fragments arranged by size: 3074 1875 1124 957 552 136 117 44 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2860 3384 3388 3392 3500 3853 4251 Size: 1255 1280 524 4 4 108 353 398 Cuts at: 4251 5906 5923 6076 6261 6706 325 Size: 1655 17 153 185 445 1498 Fragments arranged by size: 1655 1498 1280 1255 524 445 398 353 185 153 108 17 4 4 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2301 2580 4848 Size: 274 80 33 51 1443 324 279 2268 Cuts at: 4848 6125 7837 96 Size: 1277 1712 138 Fragments arranged by size: 2268 1712 1443 1277 324 279 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2945 2977 3520 3552 7839 7871 2945 Size: 32 543 32 4287 32 2953 Fragments arranged by size: 4287 2953 543 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3092 1316 Size: 1776 6103 XbaI T'CTAG_A Cuts at: 5215 5215 Size: 7879 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2953 3476 3751 4391 2953 Size: 523 275 640 6441 Fragments arranged by size: 6441 640 523 275 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7879 XmaI C'CCGG_G Cuts at: 1870 2754 3045 4162 5204 1870 Size: 884 291 1117 1042 4545 Fragments arranged by size: 4545 1117 1042 884 291 XmnI GAAnn'nnTTC Cuts at: 6008 6008 Size: 7879 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5687 276 Size: 53 83 186 936 4153 2468 Fragments arranged by size: 4153 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FseI FspI FspAI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AscI AvrII BaeI BfrBI BplI BsiWI BsmBI BstZ17I EcoRV NruI NsiI PacI PmlI PspXI PsrI RsrII SrfI SwaI