(Circular) (Six-base) MAPSORT of: pFN21ASDA0719.seq Check: 5480 from: 1 to: 6553 pFN21ASDA0719 6553 bp With 182 enzymes: SgfI * January 16, 2009 15:30 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4363 278 Size: 53 83 186 936 2827 2468 Fragments arranged by size: 2827 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3896 1937 Size: 1959 4594 Acc65I G'GTAC_C Cuts at: 3874 3874 Size: 6553 AclI AA'CG_TT Cuts at: 1563 4680 5053 1563 Size: 3117 373 3063 Fragments arranged by size: 3117 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2273 2340 2788 3012 3569 4616 6000 6268 Size: 641 67 448 224 557 1047 1384 268 Cuts at: 6268 1632 Size: 1917 Fragments arranged by size: 1917 1384 1047 641 557 448 268 224 67 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6356 AflIII A'CryG_T Cuts at: 1203 3720 5452 6429 1203 Size: 2517 1732 977 1327 Fragments arranged by size: 2517 1732 1327 977 AgeI A'CCGG_T Cuts at: 5384 5384 Size: 6553 AhdI GACnn_n'nnGTC Cuts at: 5282 5282 Size: 6553 AleI CACnn'nnGTG Cuts at: 1166 2657 1166 Size: 1491 5062 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3170 1184 Size: 1986 4567 AlwNI CAG_nnn'CTG Cuts at: 2066 4296 5868 2066 Size: 2230 1572 2751 Fragments arranged by size: 2751 2230 1572 ApaI G_GGCC'C Cuts at: 2114 2114 Size: 6553 ApaLI G'TGCA_C Cuts at: 4610 5766 4610 Size: 1156 5397 ApoI r'AATT_y Cuts at: 1493 2905 3434 3862 4088 4240 6349 1493 Size: 1412 529 428 226 152 2109 1697 Fragments arranged by size: 2109 1697 1412 529 428 226 152 AseI AT'TA_AT Cuts at: 160 1032 5107 160 Size: 872 4075 1606 Fragments arranged by size: 4075 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6553 AvaI C'yCGr_G Cuts at: 1870 1943 2113 2302 3878 1870 Size: 73 170 189 1576 4545 Fragments arranged by size: 4545 1576 189 170 73 BamHI G'GATC_C Cuts at: 3883 4250 3883 Size: 367 6186 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2161 2183 2214 3874 5329 Size: 325 217 627 374 22 31 1660 1455 Cuts at: 5329 618 Size: 1842 Fragments arranged by size: 1842 1660 1455 627 374 325 217 31 22 BanII G_rGCy'C Cuts at: 1391 2078 2114 2365 3872 1391 Size: 687 36 251 1507 4072 Fragments arranged by size: 4072 1507 687 251 36 BbeI G_GCGC'C Cuts at: 2218 2218 Size: 6553 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2279 928 Size: 971 380 5202 Fragments arranged by size: 5202 971 380 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3440 3474 4744 4778 1903 Size: 34 1503 34 1270 34 3678 Fragments arranged by size: 3678 1503 1270 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2906 4445 5655 2906 Size: 1539 1210 3804 Fragments arranged by size: 3804 1539 1210 BclI T'GATC_A Cuts at: 1548 3265 1548 Size: 1717 4836 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2137 2297 5164 Size: 107 122 71 631 605 465 160 2867 Cuts at: 5164 136 Size: 1525 Fragments arranged by size: 2867 1525 631 605 465 160 122 107 71 BglII A'GATC_T Cuts at: 3598 6548 3598 Size: 2950 3603 BlpI GC'TnA_GC Cuts at: 3970 3970 Size: 6553 Bme1580I G_kGCm'C Cuts at: 1790 2114 2120 2164 2456 4614 5770 1790 Size: 324 6 44 292 2158 1156 2573 Fragments arranged by size: 2573 2158 1156 324 292 44 6 BmgBI CAC'GTC Cuts at: 1318 2600 1318 Size: 1282 5271 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4284 5242 442 Size: 416 53 83 399 24 2867 958 1753 Fragments arranged by size: 2867 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6553 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 5213 1239 Size: 3974 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3168 4678 5543 5841 3168 Size: 1510 865 298 3880 Fragments arranged by size: 3880 1510 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5216 882 Size: 617 3717 2219 Fragments arranged by size: 3717 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3850 4344 493 Size: 711 2646 494 2702 Fragments arranged by size: 2702 2646 711 494 BsaBI GATnn'nnATC Cuts at: 3231 6547 3231 Size: 3316 3237 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2215 2241 4360 4742 Size: 53 83 186 936 682 26 2119 382 Cuts at: 4742 5377 275 Size: 635 1451 Fragments arranged by size: 2119 1451 936 682 635 382 186 83 53 26 BsaWI w'CCGG_w Cuts at: 4986 5384 5658 5805 6216 4986 Size: 398 274 147 411 5323 Fragments arranged by size: 5323 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6328 Fragments arranged by size: 6328 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6553 BseYI C'CCAG_C Cuts at: 2173 2832 5756 6252 2173 Size: 659 2924 496 2474 Fragments arranged by size: 2924 2474 659 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5980 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2157 4003 4764 5792 1540 Size: 275 186 156 1846 761 1028 2301 Fragments arranged by size: 2301 1846 1028 761 275 186 156 BsiHKAI G_wGCw'C Cuts at: 2078 2335 4614 4699 5770 2078 Size: 257 2279 85 1071 2861 Fragments arranged by size: 2861 2279 1071 257 85 BsmI GAATG_Cn' Cuts at: 2975 3226 4064 4157 2975 Size: 251 838 93 5371 Fragments arranged by size: 5371 838 251 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2078 2114 2120 2164 2335 2365 2456 Size: 399 288 36 6 44 171 30 91 Cuts at: 2456 3872 4614 4699 5770 1391 Size: 1416 742 85 1071 2174 Fragments arranged by size: 2174 1416 1071 742 399 288 171 91 85 44 36 30 6 BspEI T'CCGG_A Cuts at: 6216 6216 Size: 6553 BspHI T'CATG_A Cuts at: 4442 6172 4442 Size: 1730 4823 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3048 3908 844 Size: 2204 860 3489 Fragments arranged by size: 3489 2204 860 BsrBI CCG'CTC Cuts at: 1394 1823 4440 1394 Size: 429 2617 3507 Fragments arranged by size: 3507 2617 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3014 3058 5048 5222 65 Size: 1183 1766 44 1990 174 1396 Fragments arranged by size: 1990 1766 1396 1183 174 44 BsrFI r'CCGG_y Cuts at: 1700 1810 5197 5384 1700 Size: 110 3387 187 2869 Fragments arranged by size: 3387 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2549 3150 96 Size: 2453 601 3499 Fragments arranged by size: 3499 2453 601 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6553 BssSI C'ACGA_G Cuts at: 4613 5625 4613 Size: 1012 5541 BstAPI GCAn_nnn'nTGC Cuts at: 794 3172 794 Size: 2378 4175 BstBI TT'CG_AA Cuts at: 2007 5357 2007 Size: 3350 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6553 BstYI r'GATC_y Cuts at: 1972 3598 3883 4250 4635 4652 6104 6190 6202 Size: 1626 285 367 385 17 1452 86 12 Cuts at: 6202 6548 1972 Size: 346 1977 Fragments arranged by size: 1977 1626 1452 385 367 346 285 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2022 3672 513 Size: 552 957 1650 3394 Fragments arranged by size: 3394 1650 957 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2149 3801 487 Size: 683 56 923 1652 3239 Fragments arranged by size: 3239 1652 923 683 56 BtsI GCAGTG_nn' Cuts at: 2652 3288 4069 4863 4883 2652 Size: 636 781 794 20 4322 Fragments arranged by size: 4322 794 781 636 20 ClaI AT'CG_AT Cuts at: 1554 3210 1554 Size: 1656 4897 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3569 3604 529 Size: 35 3005 35 3478 Fragments arranged by size: 3478 3005 35 35 DraI TTT'AAA Cuts at: 2398 3857 2398 Size: 1459 5094 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5560 809 Size: 507 4244 1802 Fragments arranged by size: 4244 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2025 3744 4000 Size: 54 1425 50 275 18 195 1719 256 Cuts at: 4000 4889 6518 8 Size: 889 1629 43 Fragments arranged by size: 1719 1629 1425 889 275 256 195 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4000 1537 Size: 275 2188 4090 Fragments arranged by size: 4090 2188 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3391 3414 4483 1353 Size: 2038 23 1069 3423 Fragments arranged by size: 3423 2038 1069 23 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5140 5514 5660 387 Size: 1064 138 3551 374 146 1280 Fragments arranged by size: 3551 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2076 2076 Size: 6553 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2273 2340 2788 3012 3569 4616 5213 Size: 393 641 67 448 224 557 1047 597 Cuts at: 5213 6000 6268 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1047 787 641 597 557 448 393 268 224 67 EcoO109I rG'GnC_Cy Cuts at: 2324 2324 Size: 6553 EcoRI G'AATT_C Cuts at: 3862 4240 3862 Size: 378 6175 EcoRV GAT'ATC Cuts at: 2824 2824 Size: 6553 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3593 3593 Size: 6553 FspI TGC'GCA Cuts at: 5059 5059 Size: 6553 HaeII r_GCGC'y Cuts at: 1678 2218 5700 1678 Size: 540 3482 2531 Fragments arranged by size: 3482 2531 540 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2333 2365 2764 2796 3754 Size: 32 135 32 761 32 399 32 958 Cuts at: 3754 3786 5194 5226 5268 5300 1373 Size: 32 1408 32 42 32 2626 Fragments arranged by size: 2626 1408 958 761 399 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2473 3897 4143 669 Size: 988 281 535 1424 246 3079 Fragments arranged by size: 3079 1424 988 535 281 246 HindIII A'AGCT_T Cuts at: 748 3113 748 Size: 2365 4188 HpaI GTT'AAC Cuts at: 4143 4143 Size: 6553 KasI G'GCGC_C Cuts at: 2214 2214 Size: 6553 KpnI G_GTAC'C Cuts at: 3878 3878 Size: 6553 MfeI C'AATT_G Cuts at: 4152 4152 Size: 6553 MluI A'CGCG_T Cuts at: 6429 6429 Size: 6553 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5667 5851 1305 Size: 4362 184 2007 Fragments arranged by size: 4362 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3746 10 Size: 54 1425 343 1914 2817 Fragments arranged by size: 2817 1914 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2657 3152 3343 3719 4511 4870 5029 Size: 648 1491 495 191 376 792 359 159 Cuts at: 5029 518 Size: 2042 Fragments arranged by size: 2042 1491 792 648 495 376 359 191 159 MspA1I CmG'CkG Cuts at: 1606 2051 2066 2143 2247 2310 3250 3969 4646 Size: 445 15 77 104 63 940 719 677 Cuts at: 4646 5794 6039 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1148 940 719 677 445 245 104 77 63 15 NarI GG'CG_CC Cuts at: 2215 2215 Size: 6553 NcoI C'CATG_G Cuts at: 513 1065 2022 3672 513 Size: 552 957 1650 3394 Fragments arranged by size: 3394 1650 957 552 NdeI CA'TA_TG Cuts at: 387 3559 387 Size: 3172 3381 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6553 NotI GC'GGCC_GC Cuts at: 4000 4000 Size: 6553 NspI r_CATG'y Cuts at: 1132 1229 2698 3151 3157 3724 3911 5456 1132 Size: 97 1469 453 6 567 187 1545 2229 Fragments arranged by size: 2229 1545 1469 567 453 187 97 6 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6553 PciI A'CATG_T Cuts at: 3720 5452 3720 Size: 1732 4821 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6553 PfoI T'CCnGG_A Cuts at: 2078 2078 Size: 6553 PmeI GTTT'AAAC Cuts at: 3857 3857 Size: 6553 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4622 4654 6135 6167 4622 Size: 32 1481 32 5008 Fragments arranged by size: 5008 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2324 2324 Size: 6553 PshAI GACnn'nnGTC Cuts at: 1613 3319 1613 Size: 1706 4847 PsiI TTA'TAA Cuts at: 4123 4123 Size: 6553 PspOMI G'GGCC_C Cuts at: 2110 2110 Size: 6553 PstI C_TGCA'G Cuts at: 830 3905 830 Size: 3075 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6553 PvuII CAG'CTG Cuts at: 2143 3250 2143 Size: 1107 5446 SacI G_AGCT'C Cuts at: 2078 2078 Size: 6553 SalI G'TCGA_C Cuts at: 1936 3895 1936 Size: 1959 4594 SbfI CC_TGCA'GG Cuts at: 3905 3905 Size: 6553 ScaI AGT'ACT Cuts at: 4801 6513 4801 Size: 1712 4841 SfcI C'TryA_G Cuts at: 826 1045 2457 3901 5036 5717 5908 826 Size: 219 1412 1444 1135 681 191 1471 Fragments arranged by size: 1471 1444 1412 1135 681 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 2297 2297 Size: 6553 SfoI GGC'GCC Cuts at: 2216 2216 Size: 6553 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6553 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6553 SmaI CCC'GGG Cuts at: 1872 2115 2304 3880 1872 Size: 243 189 1576 4545 Fragments arranged by size: 4545 1576 243 189 SmlI C'TyrA_G Cuts at: 820 1017 1943 3183 4657 5558 5820 820 Size: 197 926 1240 1474 901 262 1553 Fragments arranged by size: 1553 1474 1240 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3850 4344 493 Size: 3357 494 2702 Fragments arranged by size: 3357 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 6553 SphI G_CATG'C Cuts at: 1132 2698 3911 1132 Size: 1566 1213 3774 Fragments arranged by size: 3774 1566 1213 SrfI GCCC'GGGC Cuts at: 2115 2115 Size: 6553 SspI AAT'ATT Cuts at: 5 52 2393 4477 5 Size: 47 2341 2084 2081 Fragments arranged by size: 2341 2084 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6553 StyI C'CwwG_G Cuts at: 513 1065 2022 2725 3440 3672 3992 513 Size: 552 957 703 715 232 320 3074 Fragments arranged by size: 3074 957 715 703 552 320 232 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4580 4597 4750 4935 5380 325 Size: 1255 3000 17 153 185 445 1498 Fragments arranged by size: 3000 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2549 3150 3688 Size: 274 80 33 51 1443 572 601 538 Cuts at: 3688 4799 6511 96 Size: 1111 1712 138 Fragments arranged by size: 1712 1443 1111 601 572 538 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6513 6545 6513 Size: 32 6521 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6553 XbaI T'CTAG_A Cuts at: 3889 3889 Size: 6553 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2932 3754 2932 Size: 822 5731 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6553 XmaI C'CCGG_G Cuts at: 1870 2113 2302 3878 1870 Size: 243 189 1576 4545 Fragments arranged by size: 4545 1576 243 189 XmnI GAAnn'nnTTC Cuts at: 4682 4682 Size: 6553 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4361 276 Size: 53 83 186 936 2827 2468 Fragments arranged by size: 2827 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoNI FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI PspXI PsrI RsrII SacII SanDI SapI SexAI SwaI