Restriction map for
pFN21ASDA0712
(Circular) (Six-base) MAPSORT of: pFN21ASDA0712.seq Check: 7455 from: 1 to: 9943
pFN21ASDA0712 9943 bp
With 182 enzymes: SgfI *
January 16, 2009 15:30 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 5172 5172
Size: 9943
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 7753 278
Size: 53 83 186 936 6217 2468
Fragments arranged by size:
6217 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4181 4410 7286 1937
Size: 2244 229 2876 4594
Fragments arranged by size:
4594 2876 2244 229
Acc65I G'GTAC_C
Cuts at: 7264 7264
Size: 9943
AclI AA'CG_TT
Cuts at: 1563 8070 8443 1563
Size: 6507 373 3063
Fragments arranged by size:
6507 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2072 2578 2852 3012 3533 3615 3912 3942
Size: 440 506 274 160 521 82 297 30
Cuts at: 3942 4236 4461 4545 4847 5870 8006 9390 9658
Size: 294 225 84 302 1023 2136 1384 268
Cuts at: 9658 1632
Size: 1917
Fragments arranged by size:
2136 1917 1384 1023 521 506 440 302
297 294 274 268 225 160 84 82
30
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 9746
AflIII A'CryG_T
Cuts at: 1203 8842 9819 1203
Size: 7639 977 1327
Fragments arranged by size:
7639 1327 977
AgeI A'CCGG_T
Cuts at: 8774 8774
Size: 9943
AhdI GACnn_n'nnGTC
Cuts at: 5047 8672 5047
Size: 3625 6318
AleI CACnn'nnGTG
Cuts at: 1166 5324 5585 1166
Size: 4158 261 5524
Fragments arranged by size:
5524 4158 261
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2714 6861 1184
Size: 1530 4147 4266
Fragments arranged by size:
4266 4147 1530
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4870 4902 4870
Size: 32 9911
AlwNI CAG_nnn'CTG
Cuts at: 2050 3904 4355 6368 6870 7686 9258 2050
Size: 1854 451 2013 502 816 1572 2735
Fragments arranged by size:
2735 2013 1854 1572 816 502 451
ApaI G_GGCC'C
Cuts at: 5974 6660 5974
Size: 686 9257
ApaLI G'TGCA_C
Cuts at: 8000 9156 8000
Size: 1156 8787
ApoI r'AATT_y
Cuts at: 1493 2113 2237 2369 2876 4661 7252 7478 7630
Size: 620 124 132 507 1785 2591 226 152
Cuts at: 7630 9739 1493
Size: 2109 1697
Fragments arranged by size:
2591 2109 1785 1697 620 507 226 152
132 124
AseI AT'TA_AT
Cuts at: 160 1032 8497 160
Size: 872 7465 1606
Fragments arranged by size:
7465 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9943
AvaI C'yCGr_G
Cuts at: 1870 1943 5028 5145 6108 6437 6470 7268 1870
Size: 73 3085 117 963 329 33 798 4545
Fragments arranged by size:
4545 3085 963 798 329 117 73 33
AvrII C'CTAG_G
Cuts at: 6056 6056
Size: 9943
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 5810 5843 5810
Size: 33 9910
BamHI G'GATC_C
Cuts at: 4360 7273 7640 4360
Size: 2913 367 6663
Fragments arranged by size:
6663 2913 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 5141 5537 7264 8719 618
Size: 325 217 627 3354 396 1727 1455 1842
Fragments arranged by size:
3354 1842 1727 1455 627 396 325 217
BanII G_rGCy'C
Cuts at: 1391 2554 3329 3590 5663 5974 6493 6660 7262
Size: 1163 775 261 2073 311 519 167 602
Cuts at: 7262 1391
Size: 4072
Fragments arranged by size:
4072 2073 1163 775 602 519 311 261
167
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3160 4533 5561 5643 5697 5739 6587
Size: 971 1261 1373 1028 82 54 42 848
Cuts at: 6587 928
Size: 4284
Fragments arranged by size:
4284 1373 1261 1028 971 848 82 54
42
BbvCI CC'TCA_GC
Cuts at: 3309 7074 3309
Size: 3765 6178
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5620 5654 8134 8168 1903
Size: 34 3683 34 2480 34 3678
Fragments arranged by size:
3683 3678 2480 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2755 3437 7835 9045 2755
Size: 682 4398 1210 3653
Fragments arranged by size:
4398 3653 1210 682
BclI T'GATC_A
Cuts at: 1548 4507 1548
Size: 2959 6984
BfrBI ATG'CAT
Cuts at: 2524 3776 5336 2524
Size: 1252 1560 7131
Fragments arranged by size:
7131 1560 1252
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 8554 136
Size: 107 122 71 631 605 6882 1525
Fragments arranged by size:
6882 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3161 4913 9938 3161
Size: 1752 5025 3166
Fragments arranged by size:
5025 3166 1752
BlpI GC'TnA_GC
Cuts at: 7360 7360
Size: 9943
Bme1580I G_kGCm'C
Cuts at: 1790 5146 5974 6613 6660 7125 8004 9160 1790
Size: 3356 828 639 47 465 879 1156 2573
Fragments arranged by size:
3356 2573 1156 879 828 639 465 47
BmgBI CAC'GTC
Cuts at: 1318 2254 6104 1318
Size: 936 3850 5157
Fragments arranged by size:
5157 3850 936
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4365 6972 7033
Size: 416 53 83 399 24 2948 2607 61
Cuts at: 7033 7674 8632 442
Size: 641 958 1753
Fragments arranged by size:
2948 2607 1753 958 641 416 399 83
61 53 24
BmtI G_CTAG'C
Cuts at: 1056 4112 1056
Size: 3056 6887
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5514 6273 6877 5514
Size: 759 604 8580
Fragments arranged by size:
8580 759 604
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2486 3431 3495 4286 4761 5245 6765 6847
Size: 1247 945 64 791 475 484 1520 82
Cuts at: 6847 8603 1239
Size: 1756 2579
Fragments arranged by size:
2579 1756 1520 1247 945 791 484 475
82 64
Bpu10I CC'TnA_GC
Cuts at: 2802 3309 5367 6600 7074 2802
Size: 507 2058 1233 474 5671
Fragments arranged by size:
5671 2058 1233 507 474
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3343 3604 3946 6227 8068 8933 9231 3343
Size: 261 342 2281 1841 865 298 4055
Fragments arranged by size:
4055 2281 1841 865 342 298 261
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4150 6004 8606 882
Size: 617 2651 1854 2602 2219
Fragments arranged by size:
2651 2602 2219 1854 617
BsaAI yAC'GTr
Cuts at: 493 1204 7240 7734 493
Size: 711 6036 494 2702
Fragments arranged by size:
6036 2702 711 494
BsaBI GATnn'nnATC
Cuts at: 2417 3360 9937 2417
Size: 943 6577 2423
Fragments arranged by size:
6577 2423 943
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 7750 8132 8767 275
Size: 53 83 186 936 6217 382 635 1451
Fragments arranged by size:
6217 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 5715 6240 8376 8774 9048 9195 9606 5715
Size: 525 2136 398 274 147 411 6052
Fragments arranged by size:
6052 2136 525 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 4759 4789 1178
Size: 30 165 30 3356 30 6332
Fragments arranged by size:
6332 3356 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3110 3272 3288 3534 5386 5653 6577 6943
Size: 1924 162 16 246 1852 267 924 366
Cuts at: 6943 7101 1186
Size: 158 4028
Fragments arranged by size:
4028 1924 1852 924 366 267 246 162
158 16
BseYI C'CCAG_C
Cuts at: 3205 3283 4175 4812 9146 9642 3205
Size: 78 892 637 4334 496 3506
Fragments arranged by size:
4334 3506 892 637 496 78
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2134 2847 5144 5190 5289 5744 6335
Size: 573 398 713 2297 46 99 455 591
Cuts at: 6335 1163
Size: 4771
Fragments arranged by size:
4771 2297 713 591 573 455 398 99
46
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4279 7393 8154 9182 1540
Size: 275 186 2278 3114 761 1028 2301
Fragments arranged by size:
3114 2301 2278 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2106 2253 4799 5663 6259 6606 8004 8089 9160
Size: 147 2546 864 596 347 1398 85 1071
Cuts at: 9160 2106
Size: 2889
Fragments arranged by size:
2889 2546 1398 1071 864 596 347 147
85
BsmI GAATG_Cn'
Cuts at: 2156 3778 7454 7547 2156
Size: 1622 3676 93 4552
Fragments arranged by size:
4552 3676 1622 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2106 2253 2554 3329 3590 4799 5146
Size: 399 316 147 301 775 261 1209 347
Cuts at: 5146 5663 5974 6259 6493 6606 6613 6660 7125
Size: 517 311 285 234 113 7 47 465
Cuts at: 7125 7262 8004 8089 9160 1391
Size: 137 742 85 1071 2174
Fragments arranged by size:
2174 1209 1071 775 742 517 465 399
347 316 311 301 285 261 234 147
137 113 85 47 7
BspEI T'CCGG_A
Cuts at: 5715 6240 9606 5715
Size: 525 3366 6052
Fragments arranged by size:
6052 3366 525
BspHI T'CATG_A
Cuts at: 3241 7832 9562 3241
Size: 4591 1730 3622
Fragments arranged by size:
4591 3622 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2125 2866 4933 5172 7298 844
Size: 1281 741 2067 239 2126 3489
Fragments arranged by size:
3489 2126 2067 1281 741 239
BsrBI CCG'CTC
Cuts at: 1394 1823 5862 7830 1394
Size: 429 4039 1968 3507
Fragments arranged by size:
4039 3507 1968 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5336 8438 8612 65
Size: 1183 4088 3102 174 1396
Fragments arranged by size:
4088 3102 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 8587 8774 1700
Size: 110 6777 187 2869
Fragments arranged by size:
6777 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 6078 96
Size: 5982 3961
BssHII G'CGCG_C
Cuts at: 1925 5167 1925
Size: 3242 6701
BssSI C'ACGA_G
Cuts at: 8003 9015 8003
Size: 1012 8931
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3182 794
Size: 2388 7555
BstBI TT'CG_AA
Cuts at: 2007 8747 2007
Size: 6740 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 9943
BstXI CCAn_nnnn'nTGG
Cuts at: 5230 5326 5534 6677 6743 6817 5230
Size: 96 208 1143 66 74 8356
Fragments arranged by size:
8356 1143 208 96 74 66
BstYI r'GATC_y
Cuts at: 1972 3161 3827 4360 4913 6202 6333 6364 7224
Size: 1189 666 533 553 1289 131 31 860
Cuts at: 7224 7273 7640 8025 8042 9494 9580 9592 9938
Size: 49 367 385 17 1452 86 12 346
Cuts at: 9938 1972
Size: 1977
Fragments arranged by size:
1977 1452 1289 1189 860 666 553 533
385 367 346 131 86 49 31 17
12
BstZ17I GTA'TAC
Cuts at: 4182 4182
Size: 9943
Bsu36I CC'TnA_GG
Cuts at: 3390 4029 6560 3390
Size: 639 2531 6773
Fragments arranged by size:
6773 2531 639
BtgI C'CryG_G
Cuts at: 513 1065 3042 3725 5034 5094 5562 6508 513
Size: 552 1977 683 1309 60 468 946 3948
Fragments arranged by size:
3948 1977 1309 946 683 552 468 60
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 9204
Fragments arranged by size:
9204 683 56
BtsI GCAGTG_nn'
Cuts at: 4836 5210 5272 5307 7027 7459 8253 8273 4836
Size: 374 62 35 1720 432 794 20 6506
Fragments arranged by size:
6506 1720 794 432 374 62 35 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 9943
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 9908
DraI TTT'AAA
Cuts at: 7247 7247
Size: 9943
DraIII CAC_nnn'GTG
Cuts at: 5530 5530
Size: 9943
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 7033 8950 809
Size: 507 5717 1917 1802
Fragments arranged by size:
5717 1917 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2694 5905 6679
Size: 54 1425 50 275 18 864 3211 774
Cuts at: 6679 7390 8279 9908 8
Size: 711 889 1629 43
Fragments arranged by size:
3211 1629 1425 889 864 774 711 275
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 7390 1537
Size: 275 5578 4090
Fragments arranged by size:
5578 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2496 2772 3146 3552 4148 4728 7873 1353
Size: 1143 276 374 406 596 580 3145 3423
Fragments arranged by size:
3423 3145 1143 596 580 406 374 276
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4890 5730 8530 8904 9050 387
Size: 1064 138 3301 840 2800 374 146 1280
Fragments arranged by size:
3301 2800 1280 1064 840 374 146 138
EcoICRI GAG'CTC
Cuts at: 5661 5661
Size: 9943
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2072 2486 2578 2852 3012 3431 3495
Size: 393 440 414 92 274 160 419 64
Cuts at: 3495 3533 3615 3912 3942 4236 4286 4461 4545
Size: 38 82 297 30 294 50 175 84
Cuts at: 4545 4761 4847 5245 5870 6765 6847 8006 8603
Size: 216 86 398 625 895 82 1159 597
Cuts at: 8603 9390 9658 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1159 895 787 625 597 440 419
414 398 393 297 294 274 268 216
175 160 92 86 84 82 82 64
50 38 30
EcoNI CCTnn'n_nnAGG
Cuts at: 3908 5404 5668 6832 3908
Size: 1496 264 1164 7019
Fragments arranged by size:
7019 1496 1164 264
EcoO109I rG'GnC_Cy
Cuts at: 2855 4164 4453 4725 5149 5970 5971 6275 6657
Size: 1309 289 272 424 821 1 304 382
Cuts at: 6657 6973 7152 7179 2855
Size: 316 179 27 5619
Fragments arranged by size:
5619 1309 821 424 382 316 304 289
272 179 27 1
EcoRI G'AATT_C
Cuts at: 7252 7630 7252
Size: 378 9565
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2338 2338
Size: 9943
FspI TGC'GCA
Cuts at: 5187 8449 5187
Size: 3262 6681
HaeII r_GCGC'y
Cuts at: 1678 4109 7015 9090 1678
Size: 2431 2906 2075 2531
Fragments arranged by size:
2906 2531 2431 2075
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2160 2192 3164 3196 3353
Size: 32 135 32 588 32 972 32 157
Cuts at: 3353 3385 3590 3622 3938 3970 4211 4243 4759
Size: 32 205 32 316 32 241 32 516
Cuts at: 4759 4791 5033 5065 5633 5665 6518 6550 6680
Size: 32 242 32 568 32 853 32 130
Cuts at: 6680 6712 7049 7081 8584 8616 8658 8690 1373
Size: 32 337 32 1503 32 42 32 2626
Fragments arranged by size:
2626 1503 972 853 588 568 516 337
316 242 241 205 157 135 130 42
32 32 32 32 32 32 32 32
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3319 7287 7533 669
Size: 988 281 1381 3968 246 3079
Fragments arranged by size:
3968 3079 1381 988 281 246
HindIII A'AGCT_T
Cuts at: 748 6572 748
Size: 5824 4119
HpaI GTT'AAC
Cuts at: 7533 7533
Size: 9943
KpnI G_GTAC'C
Cuts at: 7268 7268
Size: 9943
MfeI C'AATT_G
Cuts at: 2541 2817 7542 2541
Size: 276 4725 4942
Fragments arranged by size:
4942 4725 276
MluI A'CGCG_T
Cuts at: 9819 9819
Size: 9943
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2060 3169 4398 9057 9241 1305
Size: 755 1109 1229 4659 184 2007
Fragments arranged by size:
4659 2007 1229 1109 755 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2696 6681 10
Size: 54 1425 343 864 3985 3272
Fragments arranged by size:
3985 3272 1425 864 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2519 2618 2664 2696 3246 3376 4202
Size: 648 1353 99 46 32 550 130 826
Cuts at: 4202 5193 5225 5324 5585 7901 8260 8419 518
Size: 991 32 99 261 2316 359 159 2042
Fragments arranged by size:
2316 2042 1353 991 826 648 550 359
261 159 130 99 99 46 32 32
MspA1I CmG'CkG
Cuts at: 1606 2059 2359 2679 3010 3216 3308 4812 5105
Size: 453 300 320 331 206 92 1504 293
Cuts at: 5105 5155 7359 8036 9184 9429 1606
Size: 50 2204 677 1148 245 2120
Fragments arranged by size:
2204 2120 1504 1148 677 453 331 320
300 293 245 206 92 50
NcoI C'CATG_G
Cuts at: 513 1065 3042 5034 5094 5562 6508 513
Size: 552 1977 1992 60 468 946 3948
Fragments arranged by size:
3948 1992 1977 946 552 468 60
NdeI CA'TA_TG
Cuts at: 387 3883 5584 387
Size: 3496 1701 4746
Fragments arranged by size:
4746 3496 1701
NheI G'CTAG_C
Cuts at: 1052 4108 1052
Size: 3056 6887
NotI GC'GGCC_GC
Cuts at: 7390 7390
Size: 9943
NsiI A_TGCA'T
Cuts at: 2526 3778 5338 2526
Size: 1252 1560 7131
Fragments arranged by size:
7131 1560 1252
NspI r_CATG'y
Cuts at: 1132 1229 2528 5375 6163 6232 7301 8846 1132
Size: 97 1299 2847 788 69 1069 1545 2229
Fragments arranged by size:
2847 2229 1545 1299 1069 788 97 69
PasI CC'CwG_GG
Cuts at: 1348 6670 6828 1348
Size: 5322 158 4463
Fragments arranged by size:
5322 4463 158
PciI A'CATG_T
Cuts at: 8842 8842
Size: 9943
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2299 3338 3904 6964 1108
Size: 1191 1039 566 3060 4087
Fragments arranged by size:
4087 3060 1191 1039 566
PfoI T'CCnGG_A
Cuts at: 2325 3511 2325
Size: 1186 8757
PmeI GTTT'AAAC
Cuts at: 7247 7247
Size: 9943
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2785 2817 8012 8044 9525 9557 2785
Size: 32 5195 32 1481 32 3171
Fragments arranged by size:
5195 3171 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2855 4164 4453 4725 5149 6973 7152 2855
Size: 1309 289 272 424 1824 179 5646
Fragments arranged by size:
5646 1824 1309 424 289 272 179
PshAI GACnn'nnGTC
Cuts at: 1613 2485 1613
Size: 872 9071
PsiI TTA'TAA
Cuts at: 5577 7513 5577
Size: 1936 8007
PspOMI G'GGCC_C
Cuts at: 5970 6656 5970
Size: 686 9257
PspXI vC'TCGA_Gb
Cuts at: 5028 5028
Size: 9943
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2941 2973 4989 5021 2941
Size: 32 2016 32 7863
Fragments arranged by size:
7863 2016 32 32
PstI C_TGCA'G
Cuts at: 830 3009 4246 4921 6593 7295 830
Size: 2179 1237 675 1672 702 3478
Fragments arranged by size:
3478 2179 1672 1237 702 675
PvuI CG_AT'CG
Cuts at: 2001 4279 2001
Size: 2278 7665
PvuII CAG'CTG
Cuts at: 2359 3308 4812 5105 2359
Size: 949 1504 293 7197
Fragments arranged by size:
7197 1504 949 293
SacI G_AGCT'C
Cuts at: 5663 5663
Size: 9943
SalI G'TCGA_C
Cuts at: 1936 7285 1936
Size: 5349 4594
SanDI GG'GwC_CC
Cuts at: 4453 4725 5149 6973 4453
Size: 272 424 1824 7423
Fragments arranged by size:
7423 1824 424 272
SapI GCTCTTCn'nnn_
Cuts at: 3146 3146
Size: 9943
SbfI CC_TGCA'GG
Cuts at: 7295 7295
Size: 9943
ScaI AGT'ACT
Cuts at: 8191 9903 8191
Size: 1712 8231
SexAI A'CCwGG_T
Cuts at: 3909 3909
Size: 9943
SfcI C'TryA_G
Cuts at: 826 1045 2455 3005 3981 4242 4597 4917 5100
Size: 219 1410 550 976 261 355 320 183
Cuts at: 5100 5917 6457 6589 7161 7291 8426 9107 9298
Size: 817 540 132 572 130 1135 681 191
Cuts at: 9298 826
Size: 1471
Fragments arranged by size:
1471 1410 1135 976 817 681 572 550
540 355 320 261 219 191 183 132
130
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9943
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 9943
SmaI CCC'GGG
Cuts at: 1872 5147 6110 7270 1872
Size: 3275 963 1160 4545
Fragments arranged by size:
4545 3275 1160 963
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3322 3583 3961 5028 6206 8047
Size: 197 926 1379 261 378 1067 1178 1841
Cuts at: 8047 8948 9210 820
Size: 901 262 1553
Fragments arranged by size:
1841 1553 1379 1178 1067 926 901 378
262 261 197
SnaBI TAC'GTA
Cuts at: 493 7240 7734 493
Size: 6747 494 2702
Fragments arranged by size:
6747 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 9943
SphI G_CATG'C
Cuts at: 1132 2528 5375 6163 6232 7301 1132
Size: 1396 2847 788 69 1069 3774
Fragments arranged by size:
3774 2847 1396 1069 788 69
SspI AAT'ATT
Cuts at: 5 52 7867 5
Size: 47 7815 2081
Fragments arranged by size:
7815 2081 47
StuI AGG'CCT
Cuts at: 1517 2917 4262 5118 1517
Size: 1400 1345 856 6342
Fragments arranged by size:
6342 1400 1345 856
StyI C'CwwG_G
Cuts at: 513 1065 3042 3450 4800 5034 5094 5562 6056
Size: 552 1977 408 1350 234 60 468 494
Cuts at: 6056 6423 6508 7382 513
Size: 367 85 874 3074
Fragments arranged by size:
3074 1977 1350 874 552 494 468 408
367 234 85 60
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3851 6317 7970 7987 8140 8325 8770
Size: 1255 2271 2466 1653 17 153 185 445
Cuts at: 8770 325
Size: 1498
Fragments arranged by size:
2466 2271 1653 1498 1255 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2319 6078 8189
Size: 274 80 33 51 1443 342 3759 2111
Cuts at: 8189 9901 96
Size: 1712 138
Fragments arranged by size:
3759 2111 1712 1443 342 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2454 2486 3004 3036 5340 5372 9903 9935 2454
Size: 32 518 32 2304 32 4531 32 2462
Fragments arranged by size:
4531 2462 2304 518 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 9943
XbaI T'CTAG_A
Cuts at: 4252 7279 4252
Size: 3027 6916
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2737 3122 4816 5227 6689 2737
Size: 385 1694 411 1462 5991
Fragments arranged by size:
5991 1694 1462 411 385
XhoI C'TCGA_G
Cuts at: 1943 5028 1943
Size: 3085 6858
XmaI C'CCGG_G
Cuts at: 1870 5145 6108 7268 1870
Size: 3275 963 1160 4545
Fragments arranged by size:
4545 3275 1160 963
XmnI GAAnn'nnTTC
Cuts at: 4474 5694 8072 4474
Size: 1220 2378 6345
Fragments arranged by size:
6345 2378 1220
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 7751 276
Size: 53 83 186 936 6217 2468
Fragments arranged by size:
6217 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI AvrII BaeI BamHI BanI
BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI
BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NsiI NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI
PstI PvuI PvuII SacI SalI SanDI SapI SbfI
ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AscI BbeI BsiWI BsmBI EcoRV FseI FspAI
KasI NaeI NarI NgoMIV NruI PacI PmlI RsrII
SacII SfiI SfoI SrfI SwaI