(Circular) (Six-base) MAPSORT of: pFN21ASDA0693.seq Check: 1561 from: 1 to: 5917 pFN21ASDA0693 5917 bp With 182 enzymes: SgfI * January 16, 2009 15:30 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3727 278 Size: 53 83 186 936 2191 2468 Fragments arranged by size: 2468 2191 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2199 3260 1937 Size: 262 1061 4594 Fragments arranged by size: 4594 1061 262 Acc65I G'GTAC_C Cuts at: 2970 3238 2970 Size: 268 5649 AclI AA'CG_TT Cuts at: 1563 4044 4417 1563 Size: 2481 373 3063 Fragments arranged by size: 3063 2481 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3980 5364 5632 1632 Size: 2348 1384 268 1917 Fragments arranged by size: 2348 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5720 AflIII A'CryG_T Cuts at: 1203 2409 2541 4816 5793 1203 Size: 1206 132 2275 977 1327 Fragments arranged by size: 2275 1327 1206 977 132 AgeI A'CCGG_T Cuts at: 2898 4748 2898 Size: 1850 4067 AhdI GACnn_n'nnGTC Cuts at: 4646 4646 Size: 5917 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5917 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2375 1184 Size: 1191 4726 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2222 2254 2222 Size: 32 5885 AlwNI CAG_nnn'CTG Cuts at: 2182 2242 2317 2761 3660 5232 2182 Size: 60 75 444 899 1572 2867 Fragments arranged by size: 2867 1572 899 444 75 60 ApaI G_GGCC'C Cuts at: 2282 2472 2282 Size: 190 5727 ApaLI G'TGCA_C Cuts at: 2161 3974 5130 2161 Size: 1813 1156 2948 Fragments arranged by size: 2948 1813 1156 ApoI r'AATT_y Cuts at: 1493 3226 3452 3604 5713 1493 Size: 1733 226 152 2109 1697 Fragments arranged by size: 2109 1733 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4471 160 Size: 872 3439 1606 Fragments arranged by size: 3439 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5917 AvaI C'yCGr_G Cuts at: 1870 1943 3038 3242 1870 Size: 73 1095 204 4545 Fragments arranged by size: 4545 1095 204 73 BamHI G'GATC_C Cuts at: 3247 3614 3247 Size: 367 5550 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2428 2525 2719 2807 2970 Size: 325 217 627 641 97 194 88 163 Cuts at: 2970 2981 3238 4693 618 Size: 11 257 1455 1842 Fragments arranged by size: 1842 1455 641 627 325 257 217 194 163 97 88 11 BanII G_rGCy'C Cuts at: 1391 2282 2290 2472 2820 3076 3236 1391 Size: 891 8 182 348 256 160 4072 Fragments arranged by size: 4072 891 348 256 182 160 8 BbeI G_GCGC'C Cuts at: 2432 2723 2811 2432 Size: 291 88 5538 Fragments arranged by size: 5538 291 88 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4946 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4108 4142 1903 Size: 34 2171 34 3678 Fragments arranged by size: 3678 2171 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3809 5019 3809 Size: 1210 4707 BclI T'GATC_A Cuts at: 1548 1548 Size: 5917 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2391 2728 4528 Size: 107 122 71 631 605 719 337 1800 Cuts at: 4528 136 Size: 1525 Fragments arranged by size: 1800 1525 719 631 605 337 122 107 71 BglII A'GATC_T Cuts at: 5912 5912 Size: 5917 BlpI GC'TnA_GC Cuts at: 3334 3334 Size: 5917 Bme1580I G_kGCm'C Cuts at: 1790 2165 2282 2472 3978 5134 1790 Size: 375 117 190 1506 1156 2573 Fragments arranged by size: 2573 1506 1156 375 190 117 BmgBI CAC'GTC Cuts at: 1318 2607 1318 Size: 1289 4628 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2570 2941 3648 Size: 416 53 83 399 24 1153 371 707 Cuts at: 3648 4606 442 Size: 958 1753 Fragments arranged by size: 1753 1153 958 707 416 399 371 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5917 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2150 2290 2304 4577 1239 Size: 911 140 14 2273 2579 Fragments arranged by size: 2579 2273 911 140 14 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4042 4907 5205 4042 Size: 865 298 4754 Fragments arranged by size: 4754 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4580 882 Size: 617 3081 2219 Fragments arranged by size: 3081 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2410 2542 3214 3708 493 Size: 711 1206 132 672 494 2702 Fragments arranged by size: 2702 1206 711 672 494 132 BsaBI GATnn'nnATC Cuts at: 5911 5911 Size: 5917 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2343 2429 2505 2720 Size: 53 83 186 936 810 86 76 215 Cuts at: 2720 2808 3724 4106 4741 275 Size: 88 916 382 635 1451 Fragments arranged by size: 1451 936 916 810 635 382 215 186 88 86 83 76 53 BsaWI w'CCGG_w Cuts at: 2187 2898 4350 4748 5022 5169 5580 2187 Size: 711 1452 398 274 147 411 2524 Fragments arranged by size: 2524 1452 711 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2564 2594 2717 2747 1178 Size: 30 165 30 1161 30 123 30 4348 Fragments arranged by size: 4348 1161 165 123 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2359 2786 2924 1186 Size: 1173 427 138 4179 Fragments arranged by size: 4179 1173 427 138 BseYI C'CCAG_C Cuts at: 2736 2751 3006 3020 3156 5120 5616 2736 Size: 15 255 14 136 1964 496 3037 Fragments arranged by size: 3037 1964 496 255 136 15 14 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2168 2303 1163 Size: 573 432 135 4777 Fragments arranged by size: 4777 573 432 135 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2157 2214 2899 3367 4128 5156 Size: 275 186 156 57 685 468 761 1028 Cuts at: 5156 1540 Size: 2301 Fragments arranged by size: 2301 1028 761 685 468 275 186 156 57 BsiHKAI G_wGCw'C Cuts at: 2165 3136 3978 4063 5134 2165 Size: 971 842 85 1071 2948 Fragments arranged by size: 2948 1071 971 842 85 BsmI GAATG_Cn' Cuts at: 3428 3521 3428 Size: 93 5824 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2165 2282 2290 2472 2820 3076 3136 Size: 399 375 117 8 182 348 256 60 Cuts at: 3136 3236 3978 4063 5134 1391 Size: 100 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 375 348 256 182 117 100 85 60 8 BspEI T'CCGG_A Cuts at: 5580 5580 Size: 5917 BspHI T'CATG_A Cuts at: 3806 5536 3806 Size: 1730 4187 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3272 844 Size: 2428 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2301 3804 1394 Size: 429 478 1503 3507 Fragments arranged by size: 3507 1503 478 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4412 4586 65 Size: 1183 3164 174 1396 Fragments arranged by size: 3164 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2727 2898 4561 4748 1700 Size: 110 917 171 1663 187 2869 Fragments arranged by size: 2869 1663 917 187 171 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5917 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5917 BssSI C'ACGA_G Cuts at: 3977 4989 3977 Size: 1012 4905 BstAPI GCAn_nnn'nTGC Cuts at: 794 2182 2242 794 Size: 1388 60 4469 Fragments arranged by size: 4469 1388 60 BstBI TT'CG_AA Cuts at: 2007 4721 2007 Size: 2714 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5917 BstXI CCAn_nnnn'nTGG Cuts at: 2029 2029 Size: 5917 BstYI r'GATC_y Cuts at: 1972 2178 3247 3614 3999 4016 5468 5554 5566 Size: 206 1069 367 385 17 1452 86 12 Cuts at: 5566 5912 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1069 385 367 346 206 86 17 12 BstZ17I GTA'TAC Cuts at: 2200 2200 Size: 5917 BtgI C'CryG_G Cuts at: 513 1065 2028 2715 2811 2820 2848 513 Size: 552 963 687 96 9 28 3582 Fragments arranged by size: 3582 963 687 552 96 28 9 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2647 487 Size: 683 56 1421 3757 Fragments arranged by size: 3757 1421 683 56 BtsI GCAGTG_nn' Cuts at: 3433 4227 4247 3433 Size: 794 20 5103 Fragments arranged by size: 5103 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5917 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5882 DraI TTT'AAA Cuts at: 3221 3221 Size: 5917 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3148 4924 809 Size: 507 1832 1776 1802 Fragments arranged by size: 1832 1802 1776 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2048 2154 2205 Size: 54 1425 50 275 18 218 106 51 Cuts at: 2205 2601 2845 3364 4253 5882 8 Size: 396 244 519 889 1629 43 Fragments arranged by size: 1629 1425 889 519 396 275 244 218 106 54 51 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2154 3364 1537 Size: 275 342 1210 4090 Fragments arranged by size: 4090 1210 342 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2752 3847 1353 Size: 1399 1095 3423 Fragments arranged by size: 3423 1399 1095 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4504 4878 5024 387 Size: 1064 138 2915 374 146 1280 Fragments arranged by size: 2915 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2150 2290 2304 3980 4577 5364 5632 Size: 393 518 140 14 1676 597 787 268 Cuts at: 5632 1239 Size: 1524 Fragments arranged by size: 1676 1524 787 597 518 393 268 140 14 EcoNI CCTnn'n_nnAGG Cuts at: 2378 2765 2378 Size: 387 5530 EcoO109I rG'GnC_Cy Cuts at: 2279 2314 2489 2279 Size: 35 175 5707 Fragments arranged by size: 5707 175 35 EcoRI G'AATT_C Cuts at: 3226 3604 3226 Size: 378 5539 FspI TGC'GCA Cuts at: 4423 4423 Size: 5917 HaeII r_GCGC'y Cuts at: 1678 2432 2723 2811 3149 5064 1678 Size: 754 291 88 338 1915 2531 Fragments arranged by size: 2531 1915 754 338 291 88 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4558 4590 4632 4664 1373 Size: 32 135 32 2986 32 42 32 2626 Fragments arranged by size: 2986 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3261 3507 669 Size: 988 281 1323 246 3079 Fragments arranged by size: 3079 1323 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5917 HpaI GTT'AAC Cuts at: 3507 3507 Size: 5917 KasI G'GCGC_C Cuts at: 2428 2719 2807 2428 Size: 291 88 5538 Fragments arranged by size: 5538 291 88 KpnI G_GTAC'C Cuts at: 2974 3242 2974 Size: 268 5649 MfeI C'AATT_G Cuts at: 3516 3516 Size: 5917 MluI A'CGCG_T Cuts at: 5793 5793 Size: 5917 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2247 2595 5031 5215 1305 Size: 942 348 2436 184 2007 Fragments arranged by size: 2436 2007 942 348 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2207 10 Size: 54 1425 343 375 3720 Fragments arranged by size: 3720 1425 375 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2027 2453 2561 2666 2928 3875 4234 Size: 648 861 426 108 105 262 947 359 Cuts at: 4234 4393 518 Size: 159 2042 Fragments arranged by size: 2042 947 861 648 426 359 262 159 108 105 MspA1I CmG'CkG Cuts at: 1606 2710 2813 3166 3333 4010 5158 5403 1606 Size: 1104 103 353 167 677 1148 245 2120 Fragments arranged by size: 2120 1148 1104 677 353 245 167 103 NarI GG'CG_CC Cuts at: 2429 2720 2808 2429 Size: 291 88 5538 Fragments arranged by size: 5538 291 88 NcoI C'CATG_G Cuts at: 513 1065 2715 2820 2848 513 Size: 552 1650 105 28 3582 Fragments arranged by size: 3582 1650 552 105 28 NdeI CA'TA_TG Cuts at: 387 387 Size: 5917 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5917 NotI GC'GGCC_GC Cuts at: 3364 3364 Size: 5917 NspI r_CATG'y Cuts at: 1132 1229 2236 2566 3275 4820 1132 Size: 97 1007 330 709 1545 2229 Fragments arranged by size: 2229 1545 1007 709 330 97 PasI CC'CwG_GG Cuts at: 1348 3067 1348 Size: 1719 4198 PciI A'CATG_T Cuts at: 4816 4816 Size: 5917 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5917 PfoI T'CCnGG_A Cuts at: 2127 2127 Size: 5917 PmeI GTTT'AAAC Cuts at: 3221 3221 Size: 5917 PmlI CAC'GTG Cuts at: 2410 2410 Size: 5917 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3986 4018 5499 5531 3986 Size: 32 1481 32 4372 Fragments arranged by size: 4372 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2489 2489 Size: 5917 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5917 PsiI TTA'TAA Cuts at: 3487 3487 Size: 5917 PspOMI G'GGCC_C Cuts at: 2278 2468 2278 Size: 190 5727 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2183 2215 2183 Size: 32 5885 PstI C_TGCA'G Cuts at: 830 2382 3269 830 Size: 1552 887 3478 Fragments arranged by size: 3478 1552 887 PvuI CG_AT'CG Cuts at: 2001 2214 2001 Size: 213 5704 SacII CC_GC'GG Cuts at: 2814 2814 Size: 5917 SalI G'TCGA_C Cuts at: 1936 3259 1936 Size: 1323 4594 SanDI GG'GwC_CC Cuts at: 2489 2489 Size: 5917 SapI GCTCTTCn'nnn_ Cuts at: 2752 2752 Size: 5917 SbfI CC_TGCA'GG Cuts at: 2382 3269 2382 Size: 887 5030 ScaI AGT'ACT Cuts at: 2777 2834 4165 5877 2777 Size: 57 1331 1712 2817 Fragments arranged by size: 2817 1712 1331 57 SexAI A'CCwGG_T Cuts at: 2193 2193 Size: 5917 SfcI C'TryA_G Cuts at: 826 1045 2378 2476 2614 3265 4400 5081 5272 Size: 219 1333 98 138 651 1135 681 191 Cuts at: 5272 826 Size: 1471 Fragments arranged by size: 1471 1333 1135 681 651 219 191 138 98 SfoI GGC'GCC Cuts at: 2430 2721 2809 2430 Size: 291 88 5538 Fragments arranged by size: 5538 291 88 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5917 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5917 SmaI CCC'GGG Cuts at: 1872 3040 3244 1872 Size: 1168 204 4545 Fragments arranged by size: 4545 1168 204 SmlI C'TyrA_G Cuts at: 820 1017 1943 4021 4922 5184 820 Size: 197 926 2078 901 262 1553 Fragments arranged by size: 2078 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3214 3708 493 Size: 2721 494 2702 Fragments arranged by size: 2721 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 5917 SphI G_CATG'C Cuts at: 1132 3275 1132 Size: 2143 3774 SspI AAT'ATT Cuts at: 5 52 3841 5 Size: 47 3789 2081 Fragments arranged by size: 3789 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5917 StyI C'CwwG_G Cuts at: 513 1065 2715 2820 2848 3106 3356 513 Size: 552 1650 105 28 258 250 3074 Fragments arranged by size: 3074 1650 552 258 250 105 28 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3944 3961 4114 4299 4744 325 Size: 1255 2364 17 153 185 445 1498 Fragments arranged by size: 2364 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2775 2790 2832 Size: 274 80 33 51 1443 798 15 42 Cuts at: 2832 2949 4163 5875 96 Size: 117 1214 1712 138 Fragments arranged by size: 1712 1443 1214 798 274 138 117 80 51 42 33 15 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5877 5909 5877 Size: 32 5885 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5917 XbaI T'CTAG_A Cuts at: 3253 3253 Size: 5917 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2668 2668 Size: 5917 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5917 XmaI C'CCGG_G Cuts at: 1870 3038 3242 1870 Size: 1168 204 4545 Fragments arranged by size: 4545 1168 204 XmnI GAAnn'nnTTC Cuts at: 4046 4046 Size: 5917 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3725 276 Size: 53 83 186 936 2191 2468 Fragments arranged by size: 2468 2191 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI SacII SalI SanDI SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI Bsu36I DraIII EcoICRI EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PspXI PvuII RsrII SacI SfiI SrfI SwaI