Restriction map for
pFN21ASDA0677
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# NO CUT ENZYMES ( 85 enzymes) #
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AatII AccII AccIII AclI AgeI
AlwNI Aor51HI AscI AseI AsnI
AviII AvrII BamHI BbeI BbrPI
BcgI BciVI BlnI BlpI Bpu1102I
BsaAI BsaBI BseAI BsiWI BsmBI
BsmI Bsp1407I BspDI BspEI BspHI
BsrGI BssHII BstBI BstUI CelII
ClaI CpoI DraI DraIII Eco47III
EcoRV EcoT22I FseI FspI HindIII
HpaI KasI KspI MamI MfeI
MluI MroI MunI MvnI NarI
NdeI NotI NruI NsiI NspV
PacI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacII SalI SbfI SfoI
SfuI SgrAI SmaI SnaBI SphI
Sse8387I SspBI SwaI XmaCI XmaI
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# ONE CUT ENZYMES ( 48 enzymes) #
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Acc65I 412 GGTACC G^GTACC
AflII 1060 CTTAAG C^TTAAG
AflIII 1748 ACATGT A^C[GA][CT]GT
AhdI 1490 GACGCCTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 1630 GTGCAC G^TGCAC
ApaI 2348 GGGCCC GGGCC^C
ApaLI 1630 GTGCAC G^TGCAC
Asp718I 412 GGTACC G^GTACC
AspEI 1490 GACGCCTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 1784 GACGCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 349 CTCGAG C^[CT]CG[GA]G
BalI 1166 TGGCCA TGG^CCA
BbvCI 1888 GCTGAGG CCTCAGC(-5/-2)
BclI 803 TGATCA T^GATCA
BfrI 1060 CTTAAG C^TTAAG
BglII 941 AGATCT A^GATCT
BsaWI 2442 TCCGGT [AT]^CCGG[AT]
BsiEI 2547 CGGCCG CG[GA][CT]^CG
BsoBI 349 CTCGAG C^[CT]CG[GA]G
BspLU11I 1748 ACATGT A^CATGT
Bst1107I 3007 GTATAC GTA^TAC
BstEII 820 GGTTACC G^GT[ACGT]ACC
BstPI 820 GGTTACC G^GT[ACGT]ACC
BstZ17I 3007 GTATAC GTA^TAC
EagI 2547 CGGCCG C^GGCCG
Eam1105I 1490 GACGCCTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 2547 CGGCCG C^GGCCG
Eco52I 2547 CGGCCG C^GGCCG
EcoO65I 820 GGTTACC G^GT[ACGT]ACC
FbaI 803 TGATCA T^GATCA
KpnI 412 GGTACC GGTAC^C
MaeII 2384 ACGT A^CGT
MluNI 1166 TGGCCA TGG^CCA
MscI 1166 TGGCCA TGG^CCA
NheI 129 GCTAGC G^CTAGC
PaeR7I 349 CTCGAG C^TCGAG
PflFI 1784 GACGCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 2252 GGGTCCC [GA]G^G[AT]CC[CT]
ScaI 314 AGTACT AGT^ACT
SexAI 2957 ACCAGGT A^CC[AT]GGT
SfiI 1983 GGCCCAACAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SpeI 1579 ACTAGT A^CTAGT
SspI 445 AATATT AAT^ATT
StuI 1901 AGGCCT AGG^CCT
Tth111I 1784 GACGCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 3046 TCTAGA T^CTAGA
XcmI 209 CCATTCAACAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 349 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 22 enzymes) #
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AccI 861 GTCTAC GT^[AC][GT]AC
3007 GTATAC
AcyI 1490 GACGCC G[GA]^CG[CT]C
1784 GACGCC
AocI 1876 CCTGAGG CC^T[ACGT]AGG
2827 CCTGAGG
BglI 1984 GCCCAACAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2674 GCCAAGGGGGC
BpmI 2854 CTCCAG CTGGAG(16/14)
791 CTGGAG
BsaHI 1490 GACGCC G[GA]^CG[CT]C
1784 GACGCC
BspMI 596 ACCTGC ACCTGC(4/8)
2638 ACCTGC
BsrBI 352 GAGCGG CCGCTC(-3/-3)
1717 GAGCGG
BsrDI 90 CATTGC GCAATG(2/0)
2427 CATTGC
BssSI 164 CACGAG CACGAG(-5/-1)
1680 CACGAG
Bsu36I 1876 CCTGAGG CC^T[ACGT]AGG
2827 CCTGAGG
Eco81I 1876 CCTGAGG CC^T[ACGT]AGG
2827 CCTGAGG
EcoNI 2310 CCTAGAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2869 CCTGCTGAAGG
EcoRI 761 GAATTC G^AATTC
1966 GAATTC
HaeII 1696 AGCGCC [GA]GCGC^[CT]
2735 GGCGCT
Hin1I 1490 GACGCC G[GA]^CG[CT]C
1784 GACGCC
HincII 2422 GTCAAC GT[CT]^[GA]AC
2788 GTCAAC
HindII 2422 GTCAAC GT[CT]^[GA]AC
2788 GTCAAC
NaeI 452 GCCGGC GCC^GGC
829 GCCGGC
NgoMIV 452 GCCGGC G^CCGGC
829 GCCGGC
PflMI 388 CCAATCTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1081 CCAAGAGCTGG
Van91I 388 CCAATCTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1081 CCAAGAGCTGG
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# 3 OR MORE CUT ENZYMES ( 103 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI Asp700I AspHI AvaII BanI
BanII BbsI BbvI BcnI BfaI
BmyI BpuAI BsaI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I BsrFI BsrI BssKI
BstAPI BstF5I BstNI BstXI BstYI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII DrdI DsaI EaeI
EarI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HgaI
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeIII MboI
MboII MflI MnlI MseI MslI
MspA1I MspI MvaI MwoI NciI
NcoI NdeII NlaIII NlaIV NspI
PleI PstI PvuII RsaI SacI
SapI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XhoII XmnI