(Circular) (Six-base) MAPSORT of: pFN21ASDA0669.seq Check: 3687 from: 1 to: 7069 pFN21ASDA0669 7069 bp With 182 enzymes: SgfI * January 16, 2009 15:30 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2154 2175 4879 278 Size: 53 83 186 936 618 21 2704 2468 Fragments arranged by size: 2704 2468 936 618 186 83 53 21 AccI GT'mk_AC Cuts at: 1937 3587 3736 3795 4412 1937 Size: 1650 149 59 617 4594 Fragments arranged by size: 4594 1650 617 149 59 Acc65I G'GTAC_C Cuts at: 4390 4390 Size: 7069 AclI AA'CG_TT Cuts at: 1563 5196 5569 1563 Size: 3633 373 3063 Fragments arranged by size: 3633 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3890 5132 6516 6784 1632 Size: 2258 1242 1384 268 1917 Fragments arranged by size: 2258 1917 1384 1242 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6872 AflIII A'CryG_T Cuts at: 1203 2492 2564 4020 5968 6945 1203 Size: 1289 72 1456 1948 977 1327 Fragments arranged by size: 1948 1456 1327 1289 977 72 AgeI A'CCGG_T Cuts at: 5900 5900 Size: 7069 AhdI GACnn_n'nnGTC Cuts at: 5798 5798 Size: 7069 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7069 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7069 AlwNI CAG_nnn'CTG Cuts at: 2465 2992 4812 6384 2465 Size: 527 1820 1572 3150 Fragments arranged by size: 3150 1820 1572 527 ApaI G_GGCC'C Cuts at: 2736 2979 3053 2736 Size: 243 74 6752 Fragments arranged by size: 6752 243 74 ApaLI G'TGCA_C Cuts at: 5126 6282 5126 Size: 1156 5913 ApoI r'AATT_y Cuts at: 1493 4378 4604 4756 6865 1493 Size: 2885 226 152 2109 1697 Fragments arranged by size: 2885 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 5623 160 Size: 872 4591 1606 Fragments arranged by size: 4591 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7069 AvaI C'yCGr_G Cuts at: 1870 1943 2178 2264 2735 2908 2978 4394 1870 Size: 73 235 86 471 173 70 1416 4545 Fragments arranged by size: 4545 1416 471 235 173 86 73 70 BamHI G'GATC_C Cuts at: 4399 4766 4399 Size: 367 6702 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2678 3062 3221 3263 3529 Size: 325 217 627 891 384 159 42 266 Cuts at: 3529 4390 5845 618 Size: 861 1455 1842 Fragments arranged by size: 1842 1455 891 861 627 384 325 266 217 159 42 BanII G_rGCy'C Cuts at: 1391 2447 2544 2736 2979 3053 3236 4388 1391 Size: 1056 97 192 243 74 183 1152 4072 Fragments arranged by size: 4072 1152 1056 243 192 183 97 74 BbeI G_GCGC'C Cuts at: 2682 3066 3225 2682 Size: 384 159 6526 Fragments arranged by size: 6526 384 159 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3303 3981 928 Size: 971 1404 678 4016 Fragments arranged by size: 4016 1404 971 678 BbvCI CC'TCA_GC Cuts at: 3102 4357 3102 Size: 1255 5814 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3373 3407 5260 5294 1903 Size: 34 1436 34 1853 34 3678 Fragments arranged by size: 3678 1853 1436 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3108 4961 6171 3108 Size: 1853 1210 4006 Fragments arranged by size: 4006 1853 1210 BclI T'GATC_A Cuts at: 1548 2514 4240 1548 Size: 966 1726 4377 Fragments arranged by size: 4377 1726 966 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2087 2402 3071 Size: 107 122 71 631 605 415 315 669 Cuts at: 3071 3245 5680 136 Size: 174 2435 1525 Fragments arranged by size: 2435 1525 669 631 605 415 315 174 122 107 71 BglII A'GATC_T Cuts at: 7064 7064 Size: 7069 BlpI GC'TnA_GC Cuts at: 3644 4486 3644 Size: 842 6227 Bme1580I G_kGCm'C Cuts at: 1790 2736 2979 3053 3266 3396 3534 3564 5130 Size: 946 243 74 213 130 138 30 1566 Cuts at: 5130 6286 1790 Size: 1156 2573 Fragments arranged by size: 2573 1566 1156 946 243 213 138 130 74 30 BmgBI CAC'GTC Cuts at: 1318 2565 1318 Size: 1247 5822 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3451 3625 4800 Size: 416 53 83 399 24 2034 174 1175 Cuts at: 4800 5758 442 Size: 958 1753 Fragments arranged by size: 2034 1753 1175 958 416 399 174 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7069 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2170 2236 2170 Size: 66 7003 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3408 5729 1239 Size: 2169 2321 2579 Fragments arranged by size: 2579 2321 2169 Bpu10I CC'TnA_GC Cuts at: 3102 3703 3752 4357 3102 Size: 601 49 605 5814 Fragments arranged by size: 5814 605 601 49 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 5194 6059 6357 5194 Size: 865 298 5906 Fragments arranged by size: 5906 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5732 882 Size: 617 4233 2219 Fragments arranged by size: 4233 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3175 4366 4860 493 Size: 711 1971 1191 494 2702 Fragments arranged by size: 2702 1971 1191 711 494 BsaBI GATnn'nnATC Cuts at: 7063 7063 Size: 7069 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2151 2172 2679 3063 Size: 53 83 186 936 618 21 507 384 Cuts at: 3063 3222 4876 5258 5893 275 Size: 159 1654 382 635 1451 Fragments arranged by size: 1654 1451 936 635 618 507 384 382 186 159 83 53 21 BsaWI w'CCGG_w Cuts at: 2684 5502 5900 6174 6321 6732 2684 Size: 2818 398 274 147 411 3021 Fragments arranged by size: 3021 2818 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2141 2171 2325 2328 2355 Size: 30 165 30 738 30 154 3 27 Cuts at: 2355 2358 2679 2709 3402 3432 4056 4086 4318 Size: 3 321 30 693 30 624 30 232 Cuts at: 4318 4348 1178 Size: 30 3899 Fragments arranged by size: 3899 738 693 624 321 232 165 154 30 30 30 30 30 30 30 27 3 3 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2146 2216 2276 2349 2352 4345 1186 Size: 960 70 60 73 3 1993 3910 Fragments arranged by size: 3910 1993 960 73 70 60 3 BseYI C'CCAG_C Cuts at: 2433 2814 2954 3084 3315 3320 3446 3774 6272 Size: 381 140 130 231 5 126 328 2498 Cuts at: 6272 6768 2433 Size: 496 2734 Fragments arranged by size: 2734 2498 496 381 328 231 140 130 126 5 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3440 1163 Size: 573 1704 4792 Fragments arranged by size: 4792 1704 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2557 2922 4519 5280 6308 1540 Size: 275 186 556 365 1597 761 1028 2301 Fragments arranged by size: 2301 1597 1028 761 556 365 275 186 BsiHKAI G_wGCw'C Cuts at: 2422 5130 5215 6286 2422 Size: 2708 85 1071 3205 Fragments arranged by size: 3205 2708 1071 85 BsmI GAATG_Cn' Cuts at: 3280 4580 4673 3280 Size: 1300 93 5676 Fragments arranged by size: 5676 1300 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2158 2179 2225 2278 2158 Size: 21 46 53 6949 Fragments arranged by size: 6949 53 46 21 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2422 2447 2544 2736 2979 3053 3236 Size: 399 632 25 97 192 243 74 183 Cuts at: 3236 3266 3396 3534 3564 4388 5130 5215 6286 Size: 30 130 138 30 824 742 85 1071 Cuts at: 6286 1391 Size: 2174 Fragments arranged by size: 2174 1071 824 742 632 399 243 192 183 138 130 97 85 74 30 30 25 BspEI T'CCGG_A Cuts at: 6732 6732 Size: 7069 BspHI T'CATG_A Cuts at: 4958 6688 4958 Size: 1730 5339 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3171 3355 4424 844 Size: 2327 184 1069 3489 Fragments arranged by size: 3489 2327 1069 184 BsrBI CCG'CTC Cuts at: 1394 1823 2535 2662 2972 3566 4956 1394 Size: 429 712 127 310 594 1390 3507 Fragments arranged by size: 3507 1390 712 594 429 310 127 BsrDI GCAATG_nn' Cuts at: 65 1248 3846 4107 4243 5564 5738 65 Size: 1183 2598 261 136 1321 174 1396 Fragments arranged by size: 2598 1396 1321 1183 261 174 136 BsrFI r'CCGG_y Cuts at: 1700 1810 2035 3022 3179 3218 3266 5713 5900 Size: 110 225 987 157 39 48 2447 187 Cuts at: 5900 1700 Size: 2869 Fragments arranged by size: 2869 2447 987 225 187 157 110 48 39 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7069 BssHII G'CGCG_C Cuts at: 1925 3291 1925 Size: 1366 5703 BssSI C'ACGA_G Cuts at: 5129 6141 5129 Size: 1012 6057 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 7069 BstBI TT'CG_AA Cuts at: 2007 5873 2007 Size: 3866 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7069 BstXI CCAn_nnnn'nTGG Cuts at: 2383 3692 2383 Size: 1309 5760 BstYI r'GATC_y Cuts at: 1972 2612 4105 4399 4766 5151 5168 6620 6706 Size: 640 1493 294 367 385 17 1452 86 Cuts at: 6706 6718 7064 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1493 1452 640 385 367 346 294 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2199 2199 Size: 7069 BtgI C'CryG_G Cuts at: 513 1065 2076 2184 2527 3069 3240 3258 3330 Size: 552 1011 108 343 542 171 18 72 Cuts at: 3330 3525 513 Size: 195 4057 Fragments arranged by size: 4057 1011 552 542 343 195 171 108 72 18 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6330 Fragments arranged by size: 6330 683 56 BtsI GCAGTG_nn' Cuts at: 3063 3493 4308 4585 5379 5399 3063 Size: 430 815 277 794 20 4733 Fragments arranged by size: 4733 815 794 430 277 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7069 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7034 DraI TTT'AAA Cuts at: 4158 4373 4158 Size: 215 6854 DraIII CAC_nnn'GTG Cuts at: 3526 3526 Size: 7069 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2600 2767 6076 809 Size: 507 1284 167 3309 1802 Fragments arranged by size: 3309 1802 1284 507 167 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2037 2088 2554 Size: 54 1425 50 275 18 207 51 466 Cuts at: 2554 3148 3177 3246 3268 3930 4516 5405 7034 Size: 594 29 69 22 662 586 889 1629 Cuts at: 7034 8 Size: 43 Fragments arranged by size: 1629 1425 889 662 594 586 466 275 207 69 54 51 50 43 29 22 18 EagI C'GGCC_G Cuts at: 1537 1812 2554 4516 1537 Size: 275 742 1962 4090 Fragments arranged by size: 4090 1962 742 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2042 2223 2227 4999 1353 Size: 689 181 4 2772 3423 Fragments arranged by size: 3423 2772 689 181 4 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2440 3459 5656 6030 6176 387 Size: 1064 138 851 1019 2197 374 146 1280 Fragments arranged by size: 2197 1280 1064 1019 851 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3408 3890 5132 5729 6516 6784 1239 Size: 393 1776 482 1242 597 787 268 1524 Fragments arranged by size: 1776 1524 1242 787 597 482 393 268 EcoO109I rG'GnC_Cy Cuts at: 2975 3050 2975 Size: 75 6994 EcoRI G'AATT_C Cuts at: 4378 4756 4378 Size: 378 6691 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2071 2252 4209 2071 Size: 181 1957 4931 Fragments arranged by size: 4931 1957 181 FseI GG_CCGG'CC Cuts at: 3183 3183 Size: 7069 FspI TGC'GCA Cuts at: 5575 5575 Size: 7069 HaeII r_GCGC'y Cuts at: 1678 2372 2682 3066 3225 3710 6216 1678 Size: 694 310 384 159 485 2506 2531 Fragments arranged by size: 2531 2506 694 485 384 310 159 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2141 2173 2325 2357 4040 Size: 32 135 32 569 32 152 32 1683 Cuts at: 4040 4072 4232 4264 5710 5742 5784 5816 1373 Size: 32 160 32 1446 32 42 32 2626 Fragments arranged by size: 2626 1683 1446 569 160 152 135 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3796 3912 4413 4659 669 Size: 988 281 1858 116 501 246 3079 Fragments arranged by size: 3079 1858 988 501 281 246 116 HindIII A'AGCT_T Cuts at: 748 748 Size: 7069 HpaI GTT'AAC Cuts at: 3912 4659 3912 Size: 747 6322 KasI G'GCGC_C Cuts at: 2678 3062 3221 2678 Size: 384 159 6526 Fragments arranged by size: 6526 384 159 KpnI G_GTAC'C Cuts at: 4394 4394 Size: 7069 MfeI C'AATT_G Cuts at: 4668 4668 Size: 7069 MluI A'CGCG_T Cuts at: 6945 6945 Size: 7069 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2766 6183 6367 1305 Size: 1461 3417 184 2007 Fragments arranged by size: 3417 2007 1461 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2090 3932 10 Size: 54 1425 343 258 1842 3147 Fragments arranged by size: 3147 1842 1425 343 258 54 MslI CAynn'nnrTG Cuts at: 518 1166 5027 5386 5545 518 Size: 648 3861 359 159 2042 Fragments arranged by size: 3861 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2302 2309 2670 2896 3071 3496 3643 4485 Size: 696 7 361 226 175 425 147 842 Cuts at: 4485 5162 6310 6555 1606 Size: 677 1148 245 2120 Fragments arranged by size: 2120 1148 842 696 677 425 361 245 226 175 147 7 NaeI GCC'GGC Cuts at: 2037 3024 3181 2037 Size: 987 157 5925 Fragments arranged by size: 5925 987 157 NarI GG'CG_CC Cuts at: 2679 3063 3222 2679 Size: 384 159 6526 Fragments arranged by size: 6526 384 159 NcoI C'CATG_G Cuts at: 513 1065 3330 513 Size: 552 2265 4252 Fragments arranged by size: 4252 2265 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7069 NgoMIV G'CCGG_C Cuts at: 2035 3022 3179 2035 Size: 987 157 5925 Fragments arranged by size: 5925 987 157 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7069 NotI GC'GGCC_GC Cuts at: 4516 4516 Size: 7069 NspI r_CATG'y Cuts at: 1132 1229 2496 3692 4427 5972 1132 Size: 97 1267 1196 735 1545 2229 Fragments arranged by size: 2229 1545 1267 1196 735 97 PasI CC'CwG_GG Cuts at: 1348 2965 1348 Size: 1617 5452 PciI A'CATG_T Cuts at: 2492 5968 2492 Size: 3476 3593 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 7069 PmeI GTTT'AAAC Cuts at: 4373 4373 Size: 7069 PmlI CAC'GTG Cuts at: 3175 3175 Size: 7069 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5138 5170 6651 6683 5138 Size: 32 1481 32 5524 Fragments arranged by size: 5524 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7069 PsiI TTA'TAA Cuts at: 4639 4639 Size: 7069 PspOMI G'GGCC_C Cuts at: 2732 2975 3049 2732 Size: 243 74 6752 Fragments arranged by size: 6752 243 74 PstI C_TGCA'G Cuts at: 830 2470 3058 3168 3189 3556 4421 830 Size: 1640 588 110 21 367 865 3478 Fragments arranged by size: 3478 1640 865 588 367 110 21 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7069 PvuII CAG'CTG Cuts at: 2302 3643 2302 Size: 1341 5728 SacII CC_GC'GG Cuts at: 3072 3072 Size: 7069 SalI G'TCGA_C Cuts at: 1936 3794 4411 1936 Size: 1858 617 4594 Fragments arranged by size: 4594 1858 617 SapI GCTCTTCn'nnn_ Cuts at: 2042 2227 2042 Size: 185 6884 SbfI CC_TGCA'GG Cuts at: 4421 4421 Size: 7069 ScaI AGT'ACT Cuts at: 2641 5317 7029 2641 Size: 2676 1712 2681 Fragments arranged by size: 2681 2676 1712 SexAI A'CCwGG_T Cuts at: 3508 3508 Size: 7069 SfcI C'TryA_G Cuts at: 826 1045 2466 2545 3054 3164 3185 3552 4417 Size: 219 1421 79 509 110 21 367 865 Cuts at: 4417 5552 6233 6424 826 Size: 1135 681 191 1471 Fragments arranged by size: 1471 1421 1135 865 681 509 367 219 191 110 79 21 SfiI GGCCn_nnn'nGGCC Cuts at: 2087 2087 Size: 7069 SfoI GGC'GCC Cuts at: 2680 3064 3223 2680 Size: 384 159 6526 Fragments arranged by size: 6526 384 159 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7069 SgrAI Cr'CCGG_yG Cuts at: 1700 3218 1700 Size: 1518 5551 SmaI CCC'GGG Cuts at: 1872 2266 2910 2980 4396 1872 Size: 394 644 70 1416 4545 Fragments arranged by size: 4545 1416 644 394 70 SmlI C'TyrA_G Cuts at: 820 1017 1943 5173 6074 6336 820 Size: 197 926 3230 901 262 1553 Fragments arranged by size: 3230 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4366 4860 493 Size: 3873 494 2702 Fragments arranged by size: 3873 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 7069 SphI G_CATG'C Cuts at: 1132 4427 1132 Size: 3295 3774 SspI AAT'ATT Cuts at: 5 52 4993 5 Size: 47 4941 2081 Fragments arranged by size: 4941 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7069 StyI C'CwwG_G Cuts at: 513 1065 3330 4508 513 Size: 552 2265 1178 3074 Fragments arranged by size: 3074 2265 1178 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3422 3470 5096 5113 5266 5451 5896 Size: 1255 1842 48 1626 17 153 185 445 Cuts at: 5896 325 Size: 1498 Fragments arranged by size: 1842 1626 1498 1255 445 185 153 48 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2639 5315 7027 Size: 274 80 33 51 1443 662 2676 1712 Cuts at: 7027 96 Size: 138 Fragments arranged by size: 2676 1712 1443 662 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2679 2711 3402 3434 7029 7061 2679 Size: 32 691 32 3595 32 2687 Fragments arranged by size: 3595 2687 691 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7069 XbaI T'CTAG_A Cuts at: 4405 4405 Size: 7069 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2083 3256 3328 2083 Size: 1173 72 5824 Fragments arranged by size: 5824 1173 72 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7069 XmaI C'CCGG_G Cuts at: 1870 2264 2908 2978 4394 1870 Size: 394 644 70 1416 4545 Fragments arranged by size: 4545 1416 644 394 70 XmnI GAAnn'nnTTC Cuts at: 5198 5198 Size: 7069 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2152 2173 4877 276 Size: 53 83 186 936 618 21 2704 2468 Fragments arranged by size: 2704 2468 936 618 186 83 53 21 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FseI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacII SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BfrBI BsiWI BstZ17I EcoICRI EcoNI EcoRV FspAI NruI NsiI PacI PfoI PpuMI PspXI PsrI RsrII SacI SanDI SrfI SwaI