Restriction map for
pFN21ASDA0668
(Circular) (Six-base) MAPSORT of: pFN21ASDA0668.seq Check: 5952 from: 1 to: 6880
pFN21ASDA0668 6880 bp
With 182 enzymes: SgfI *
January 16, 2009 15:30 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3592 4142 3592
Size: 550 6330
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2622 4690 278
Size: 53 83 186 936 1086 2068 2468
Fragments arranged by size:
2468 2068 1086 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4223 1937
Size: 2286 4594
Acc65I G'GTAC_C
Cuts at: 2586 3768 4201 2586
Size: 1182 433 5265
Fragments arranged by size:
5265 1182 433
AclI AA'CG_TT
Cuts at: 1563 5007 5380 1563
Size: 3444 373 3063
Fragments arranged by size:
3444 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2536 2672 3071 3215 3332 3434 4132 4943
Size: 904 136 399 144 117 102 698 811
Cuts at: 4943 6327 6595 1632
Size: 1384 268 1917
Fragments arranged by size:
1917 1384 904 811 698 399 268 144
136 117 102
AfeI AGC'GCT
Cuts at: 3635 3635
Size: 6880
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6683
AflIII A'CryG_T
Cuts at: 1203 5779 6756 1203
Size: 4576 977 1327
Fragments arranged by size:
4576 1327 977
AgeI A'CCGG_T
Cuts at: 5711 5711
Size: 6880
AhdI GACnn_n'nnGTC
Cuts at: 5609 5609
Size: 6880
AleI CACnn'nnGTG
Cuts at: 1166 3506 1166
Size: 2340 4540
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6880
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3374 3406 3374
Size: 32 6848
AlwNI CAG_nnn'CTG
Cuts at: 3193 3295 3628 4623 6195 3193
Size: 102 333 995 1572 3878
Fragments arranged by size:
3878 1572 995 333 102
ApaI G_GGCC'C
Cuts at: 2204 2204
Size: 6880
ApaLI G'TGCA_C
Cuts at: 3605 3809 4937 6093 3605
Size: 204 1128 1156 4392
Fragments arranged by size:
4392 1156 1128 204
ApoI r'AATT_y
Cuts at: 1493 2900 4189 4415 4567 6676 1493
Size: 1407 1289 226 152 2109 1697
Fragments arranged by size:
2109 1697 1407 1289 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5434 160
Size: 872 4402 1606
Fragments arranged by size:
4402 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6880
AvaI C'yCGr_G
Cuts at: 1870 1943 2416 2856 3420 3786 4124 4205 1870
Size: 73 473 440 564 366 338 81 4545
Fragments arranged by size:
4545 564 473 440 366 338 81 73
BamHI G'GATC_C
Cuts at: 4101 4210 4577 4101
Size: 109 367 6404
Fragments arranged by size:
6404 367 109
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2576 2586 3768 4201 5656
Size: 325 217 627 789 10 1182 433 1455
Cuts at: 5656 618
Size: 1842
Fragments arranged by size:
1842 1455 1182 789 627 433 325 217
10
BanII G_rGCy'C
Cuts at: 1391 2099 2204 2276 2411 2513 2965 3242 3323
Size: 708 105 72 135 102 452 277 81
Cuts at: 3323 3447 4167 4199 1391
Size: 124 720 32 4072
Fragments arranged by size:
4072 720 708 452 277 135 124 105
102 81 72 32
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2614 2660 3141 3987 928
Size: 971 715 46 481 846 3821
Fragments arranged by size:
3821 971 846 715 481 46
BbvCI CC'TCA_GC
Cuts at: 3254 3743 3254
Size: 489 6391
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5071 5105 1903
Size: 34 3134 34 3678
Fragments arranged by size:
3678 3134 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4772 5982 4772
Size: 1210 5670
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6880
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2545 3753 5491
Size: 107 122 71 631 605 873 1208 1738
Cuts at: 5491 136
Size: 1525
Fragments arranged by size:
1738 1525 1208 873 631 605 122 107
71
BglII A'GATC_T
Cuts at: 6875 6875
Size: 6880
BlpI GC'TnA_GC
Cuts at: 2838 3471 4297 2838
Size: 633 826 5421
Fragments arranged by size:
5421 826 633
Bme1580I G_kGCm'C
Cuts at: 1790 2204 3609 3813 3912 3949 4941 6097 1790
Size: 414 1405 204 99 37 992 1156 2573
Fragments arranged by size:
2573 1405 1156 992 414 204 99 37
BmgBI CAC'GTC
Cuts at: 1318 2694 3379 1318
Size: 1376 685 4819
Fragments arranged by size:
4819 1376 685
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2314 3772 4123
Size: 416 53 83 399 24 897 1458 351
Cuts at: 4123 4611 5569 442
Size: 488 958 1753
Fragments arranged by size:
1753 1458 958 897 488 416 399 351
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6880
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3812 3812
Size: 6880
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2300 2891 2966 3038 3284 3506 3695 3782
Size: 1061 591 75 72 246 222 189 87
Cuts at: 3782 3925 5540 1239
Size: 143 1615 2579
Fragments arranged by size:
2579 1615 1061 591 246 222 189 143
87 75 72
Bpu10I CC'TnA_GC
Cuts at: 2990 3254 3554 3743 2990
Size: 264 300 189 6127
Fragments arranged by size:
6127 300 264 189
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5005 5870 6168 5005
Size: 865 298 5717
Fragments arranged by size:
5717 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3710 3719 5543 882
Size: 617 2211 9 1824 2219
Fragments arranged by size:
2219 2211 1824 617 9
BsaAI yAC'GTr
Cuts at: 493 1204 2443 4080 4177 4671 493
Size: 711 1239 1637 97 494 2702
Fragments arranged by size:
2702 1637 1239 711 494 97
BsaBI GATnn'nnATC
Cuts at: 6874 6874
Size: 6880
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2298 2619 2878 4687
Size: 53 83 186 936 765 321 259 1809
Cuts at: 4687 5069 5704 275
Size: 382 635 1451
Fragments arranged by size:
1809 1451 936 765 635 382 321 259
186 83 53
BsaWI w'CCGG_w
Cuts at: 5313 5711 5985 6132 6543 5313
Size: 398 274 147 411 5650
Fragments arranged by size:
5650 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6655
Fragments arranged by size:
6655 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2344 2347 2416 3473 3611 3731 1186
Size: 1158 3 69 1057 138 120 4335
Fragments arranged by size:
4335 1158 1057 138 120 69 3
BseYI C'CCAG_C
Cuts at: 2105 2185 2197 2802 3351 3825 6083 6579 2105
Size: 80 12 605 549 474 2258 496 2406
Fragments arranged by size:
2406 2258 605 549 496 474 80 12
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 6307
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4330 5091 6119 1540
Size: 275 186 2329 761 1028 2301
Fragments arranged by size:
2329 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2099 2120 2276 3242 3323 3609 3813 4941 5026
Size: 21 156 966 81 286 204 1128 85
Cuts at: 5026 6097 2099
Size: 1071 2882
Fragments arranged by size:
2882 1128 1071 966 286 204 156 85
81 21
BsmI GAATG_Cn'
Cuts at: 4391 4484 4391
Size: 93 6787
BsmBI CGTCTCn'nnnn_
Cuts at: 2503 2503
Size: 6880
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2099 2120 2204 2276 2411 2513 2965
Size: 399 309 21 84 72 135 102 452
Cuts at: 2965 3242 3323 3447 3609 3813 3912 3949 4167
Size: 277 81 124 162 204 99 37 218
Cuts at: 4167 4199 4941 5026 6097 1391
Size: 32 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 452 399 309 277 218
204 162 135 124 102 99 85 84
81 72 37 32 21
BspEI T'CCGG_A
Cuts at: 6543 6543
Size: 6880
BspHI T'CATG_A
Cuts at: 4769 6499 4769
Size: 1730 5150
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2325 3592 3993 4142 4235 844
Size: 1481 1267 401 149 93 3489
Fragments arranged by size:
3489 1481 1267 401 149 93
BsrBI CCG'CTC
Cuts at: 1394 1823 2875 4767 1394
Size: 429 1052 1892 3507
Fragments arranged by size:
3507 1892 1052 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5375 5549 65
Size: 1183 4127 174 1396
Fragments arranged by size:
4127 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2544 3278 5524 5711 1700
Size: 110 734 734 2246 187 2869
Fragments arranged by size:
2869 2246 734 734 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6880
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6880
BssSI C'ACGA_G
Cuts at: 2249 2996 4940 5952 2249
Size: 747 1944 1012 3177
Fragments arranged by size:
3177 1944 1012 747
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3628 794
Size: 2834 4046
BstBI TT'CG_AA
Cuts at: 2007 5684 2007
Size: 3677 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6880
BstXI CCAn_nnnn'nTGG
Cuts at: 2935 2978 2935
Size: 43 6837
BstYI r'GATC_y
Cuts at: 1972 3357 3495 4101 4210 4577 4962 4979 6431
Size: 1385 138 606 109 367 385 17 1452
Cuts at: 6431 6517 6529 6875 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 1385 606 385 367 346 138
109 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 3543 3855 513
Size: 552 2478 312 3538
Fragments arranged by size:
3538 2478 552 312
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2805 487
Size: 683 56 1579 4562
Fragments arranged by size:
4562 1579 683 56
BtsI GCAGTG_nn'
Cuts at: 4396 5190 5210 4396
Size: 794 20 6066
Fragments arranged by size:
6066 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6880
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6845
DraI TTT'AAA
Cuts at: 2128 4184 2128
Size: 2056 4824
DraIII CAC_nnn'GTG
Cuts at: 3616 3616
Size: 6880
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3223 5887 809
Size: 507 1907 2664 1802
Fragments arranged by size:
2664 1907 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2529 2968 3276
Size: 54 1425 50 275 18 699 439 308
Cuts at: 3276 3531 4074 4327 5216 6845 8
Size: 255 543 253 889 1629 43
Fragments arranged by size:
1629 1425 889 699 543 439 308 275
255 253 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4327 1537
Size: 275 2515 4090
Fragments arranged by size:
4090 2515 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2147 2416 2555 3146 3755 4810 1353
Size: 794 269 139 591 609 1055 3423
Fragments arranged by size:
3423 1055 794 609 591 269 139
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3344 3851 3869 5467 5841 5987
Size: 1064 138 1755 507 18 1598 374 146
Cuts at: 5987 387
Size: 1280
Fragments arranged by size:
1755 1598 1280 1064 507 374 146 138
18
EcoICRI GAG'CTC
Cuts at: 2097 2274 3240 3321 2097
Size: 177 966 81 5656
Fragments arranged by size:
5656 966 177 81
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2300 2536 2672 2891 2966 3038 3071
Size: 393 668 236 136 219 75 72 33
Cuts at: 3071 3215 3284 3332 3434 3506 3695 3782 3925
Size: 144 69 48 102 72 189 87 143
Cuts at: 3925 4132 4943 5540 6327 6595 1239
Size: 207 811 597 787 268 1524
Fragments arranged by size:
1524 811 787 668 597 393 268 236
219 207 189 144 143 136 102 87
75 72 72 69 48 33
EcoNI CCTnn'n_nnAGG
Cuts at: 2152 3053 3287 3989 2152
Size: 901 234 702 5043
Fragments arranged by size:
5043 901 702 234
EcoO109I rG'GnC_Cy
Cuts at: 2145 2314 3625 4063 2145
Size: 169 1311 438 4962
Fragments arranged by size:
4962 1311 438 169
EcoRI G'AATT_C
Cuts at: 4189 4567 4189
Size: 378 6502
FseI GG_CCGG'CC
Cuts at: 3282 3282
Size: 6880
FspI TGC'GCA
Cuts at: 5386 5386
Size: 6880
HaeII r_GCGC'y
Cuts at: 1678 2044 2179 2192 2496 2641 3014 3637 6027
Size: 366 135 13 304 145 373 623 2390
Cuts at: 6027 1678
Size: 2531
Fragments arranged by size:
2531 2390 623 373 366 304 145 135
13
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3368 3400 3454 3486 5521
Size: 32 135 32 1796 32 54 32 2035
Cuts at: 5521 5553 5595 5627 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2035 1796 135 54 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4224 4470 669
Size: 988 281 2286 246 3079
Fragments arranged by size:
3079 2286 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6880
HpaI GTT'AAC
Cuts at: 4470 4470
Size: 6880
KpnI G_GTAC'C
Cuts at: 2590 3772 4205 2590
Size: 1182 433 5265
Fragments arranged by size:
5265 1182 433
MfeI C'AATT_G
Cuts at: 4479 4479
Size: 6880
MluI A'CGCG_T
Cuts at: 6756 6756
Size: 6880
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2934 5994 6178 1305
Size: 1629 3060 184 2007
Fragments arranged by size:
3060 2007 1629 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2531 2970 4076 10
Size: 54 1425 343 699 439 1106 2814
Fragments arranged by size:
2814 1425 1106 699 439 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3506 4838 5197 5356 518
Size: 648 2340 1332 359 159 2042
Fragments arranged by size:
2340 2042 1332 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2088 2197 2370 2603 2680 3193 3274 3475
Size: 482 109 173 233 77 513 81 201
Cuts at: 3475 3545 4296 4973 6121 6366 1606
Size: 70 751 677 1148 245 2120
Fragments arranged by size:
2120 1148 751 677 513 482 245 233
201 173 109 81 77 70
NaeI GCC'GGC
Cuts at: 2546 3280 2546
Size: 734 6146
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 6328
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6880
NgoMIV G'CCGG_C
Cuts at: 2544 3278 2544
Size: 734 6146
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6880
NotI GC'GGCC_GC
Cuts at: 4327 4327
Size: 6880
NspI r_CATG'y
Cuts at: 1132 1229 3908 4238 5783 1132
Size: 97 2679 330 1545 2229
Fragments arranged by size:
2679 2229 1545 330 97
PasI CC'CwG_GG
Cuts at: 1348 2059 1348
Size: 711 6169
PciI A'CATG_T
Cuts at: 5779 5779
Size: 6880
PflMI CCAn_nnn'nTGG
Cuts at: 1108 4090 1108
Size: 2982 3898
PfoI T'CCnGG_A
Cuts at: 3087 3231 3087
Size: 144 6736
PmeI GTTT'AAAC
Cuts at: 4184 4184
Size: 6880
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2243 2275 4949 4981 6462 6494 2243
Size: 32 2674 32 1481 32 2629
Fragments arranged by size:
2674 2629 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2145 2314 2145
Size: 169 6711
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6880
PsiI TTA'TAA
Cuts at: 4450 4450
Size: 6880
PspOMI G'GGCC_C
Cuts at: 2200 2200
Size: 6880
PstI C_TGCA'G
Cuts at: 830 2739 3288 3570 3633 3990 4232 830
Size: 1909 549 282 63 357 242 3478
Fragments arranged by size:
3478 1909 549 357 282 242 63
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6880
PvuII CAG'CTG
Cuts at: 2197 2603 3193 3274 3475 2197
Size: 406 590 81 201 5602
Fragments arranged by size:
5602 590 406 201 81
SacI G_AGCT'C
Cuts at: 2099 2276 3242 3323 2099
Size: 177 966 81 5656
Fragments arranged by size:
5656 966 177 81
SacII CC_GC'GG
Cuts at: 3546 3546
Size: 6880
SalI G'TCGA_C
Cuts at: 1936 4222 1936
Size: 2286 4594
SapI GCTCTTCn'nnn_
Cuts at: 2416 2555 2416
Size: 139 6741
SbfI CC_TGCA'GG
Cuts at: 4232 4232
Size: 6880
ScaI AGT'ACT
Cuts at: 5128 6840 5128
Size: 1712 5168
SexAI A'CCwGG_T
Cuts at: 2582 4083 2582
Size: 1501 5379
SfcI C'TryA_G
Cuts at: 826 1045 2230 2735 3284 3566 3629 3637 3986
Size: 219 1185 505 549 282 63 8 349
Cuts at: 3986 4228 5363 6044 6235 826
Size: 242 1135 681 191 1471
Fragments arranged by size:
1471 1185 1135 681 549 505 349 282
242 219 191 63 8
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6880
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6880
SmaI CCC'GGG
Cuts at: 1872 2858 4207 1872
Size: 986 1349 4545
Fragments arranged by size:
4545 1349 986
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4984 5885 6147 820
Size: 197 926 3041 901 262 1553
Fragments arranged by size:
3041 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4177 4671 493
Size: 3684 494 2702
Fragments arranged by size:
3684 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6880
SphI G_CATG'C
Cuts at: 1132 4238 1132
Size: 3106 3774
SspI AAT'ATT
Cuts at: 5 52 4804 5
Size: 47 4752 2081
Fragments arranged by size:
4752 2081 47
StuI AGG'CCT
Cuts at: 1517 3917 1517
Size: 2400 4480
StyI C'CwwG_G
Cuts at: 513 1065 3912 3951 4006 4319 513
Size: 552 2847 39 55 313 3074
Fragments arranged by size:
3074 2847 552 313 55 39
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2281 3518 3828 3940 4907 4924 5077
Size: 1255 701 1237 310 112 967 17 153
Cuts at: 5077 5262 5707 325
Size: 185 445 1498
Fragments arranged by size:
1498 1255 1237 967 701 445 310 185
153 112 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4128 5126 6838
Size: 274 80 33 51 1443 2151 998 1712
Cuts at: 6838 96
Size: 138
Fragments arranged by size:
2151 1712 1443 998 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6840 6872 6840
Size: 32 6848
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6880
XbaI T'CTAG_A
Cuts at: 4216 4216
Size: 6880
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2275 2374 2978 3822 4084 2275
Size: 99 604 844 262 5071
Fragments arranged by size:
5071 844 604 262 99
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6880
XmaI C'CCGG_G
Cuts at: 1870 2856 4205 1870
Size: 986 1349 4545
Fragments arranged by size:
4545 1349 986
XmnI GAAnn'nnTTC
Cuts at: 2657 5009 2657
Size: 2352 4528
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2620 4688 276
Size: 53 83 186 936 1086 2068 2468
Fragments arranged by size:
2468 2068 1086 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FseI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI
SacII SalI SapI SbfI ScaI SexAI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AscI AvrII BaeI BbeI BfrBI BsiWI BstZ17I Bsu36I
EcoRV FalI FspAI KasI NarI NruI NsiI PacI
PmlI PspXI PsrI RsrII SanDI SfiI SfoI SrfI
SwaI