(Circular) (Six-base) MAPSORT of: pFN21ASDA0663.seq Check: 5499 from: 1 to: 7159 pFN21ASDA0663 7159 bp With 182 enzymes: SgfI * January 16, 2009 15:30 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4969 278 Size: 53 83 186 936 3433 2468 Fragments arranged by size: 3433 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4502 1937 Size: 2565 4594 Acc65I G'GTAC_C Cuts at: 4480 4480 Size: 7159 AclI AA'CG_TT Cuts at: 1563 5286 5659 1563 Size: 3723 373 3063 Fragments arranged by size: 3723 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2601 2715 3183 3575 3633 4194 4209 5222 Size: 969 114 468 392 58 561 15 1013 Cuts at: 5222 6606 6874 1632 Size: 1384 268 1917 Fragments arranged by size: 1917 1384 1013 969 561 468 392 268 114 58 15 AflII C'TTAA_G Cuts at: 820 1017 3014 820 Size: 197 1997 4965 Fragments arranged by size: 4965 1997 197 AflIII A'CryG_T Cuts at: 1203 2066 6058 7035 1203 Size: 863 3992 977 1327 Fragments arranged by size: 3992 1327 977 863 AgeI A'CCGG_T Cuts at: 5990 5990 Size: 7159 AhdI GACnn_n'nnGTC Cuts at: 5888 5888 Size: 7159 AleI CACnn'nnGTG Cuts at: 1166 3821 1166 Size: 2655 4504 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7159 AlwNI CAG_nnn'CTG Cuts at: 2777 4166 4902 6474 2777 Size: 1389 736 1572 3462 Fragments arranged by size: 3462 1572 1389 736 ApaLI G'TGCA_C Cuts at: 3428 5216 6372 3428 Size: 1788 1156 4215 Fragments arranged by size: 4215 1788 1156 ApoI r'AATT_y Cuts at: 1493 2211 2653 2885 3111 3681 4468 4694 4846 Size: 718 442 232 226 570 787 226 152 Cuts at: 4846 6955 1493 Size: 2109 1697 Fragments arranged by size: 2109 1697 787 718 570 442 232 226 226 152 AseI AT'TA_AT Cuts at: 160 1032 2493 5713 160 Size: 872 1461 3220 1606 Fragments arranged by size: 3220 1606 1461 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7159 AvaI C'yCGr_G Cuts at: 1870 1943 4484 1870 Size: 73 2541 4545 Fragments arranged by size: 4545 2541 73 BamHI G'GATC_C Cuts at: 4396 4489 4856 4396 Size: 93 367 6699 Fragments arranged by size: 6699 367 93 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3840 4480 5935 618 Size: 325 217 627 2053 640 1455 1842 Fragments arranged by size: 2053 1842 1455 640 627 325 217 BanII G_rGCy'C Cuts at: 1391 2766 3488 4478 1391 Size: 1375 722 990 4072 Fragments arranged by size: 4072 1375 990 722 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2046 3104 928 Size: 971 147 1058 4983 Fragments arranged by size: 4983 1058 971 147 BbvCI CC'TCA_GC Cuts at: 2421 2421 Size: 7159 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2151 2185 5350 5384 1903 Size: 34 214 34 3165 34 3678 Fragments arranged by size: 3678 3165 214 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3298 5051 6261 3298 Size: 1753 1210 4196 Fragments arranged by size: 4196 1753 1210 BclI T'GATC_A Cuts at: 1548 2524 1548 Size: 976 6183 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4118 5770 136 Size: 107 122 71 631 605 2446 1652 1525 Fragments arranged by size: 2446 1652 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 7154 7154 Size: 7159 BlpI GC'TnA_GC Cuts at: 4576 4576 Size: 7159 Bme1580I G_kGCm'C Cuts at: 1790 3432 5220 6376 1790 Size: 1642 1788 1156 2573 Fragments arranged by size: 2573 1788 1642 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7159 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2882 4210 4890 Size: 416 53 83 399 24 1465 1328 680 Cuts at: 4890 5848 442 Size: 958 1753 Fragments arranged by size: 1753 1465 1328 958 680 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2370 1056 Size: 1314 5845 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2946 3479 3513 4069 5819 1239 Size: 1707 533 34 556 1750 2579 Fragments arranged by size: 2579 1750 1707 556 533 34 Bpu10I CC'TnA_GC Cuts at: 2421 2760 2421 Size: 339 6820 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2693 2982 3146 4448 5284 6149 6447 2693 Size: 289 164 1302 836 865 298 3405 Fragments arranged by size: 3405 1302 865 836 298 289 164 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3650 3695 5822 882 Size: 617 2151 45 2127 2219 Fragments arranged by size: 2219 2151 2127 617 45 BsaAI yAC'GTr Cuts at: 493 1204 4456 4950 493 Size: 711 3252 494 2702 Fragments arranged by size: 3252 2702 711 494 BsaBI GATnn'nnATC Cuts at: 2523 7153 2523 Size: 4630 2529 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4966 5348 5983 275 Size: 53 83 186 936 3433 382 635 1451 Fragments arranged by size: 3433 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2613 5592 5990 6264 6411 6822 2613 Size: 2979 398 274 147 411 2950 Fragments arranged by size: 2979 2950 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6934 Fragments arranged by size: 6934 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3205 3407 1186 Size: 2019 202 4938 Fragments arranged by size: 4938 2019 202 BseYI C'CCAG_C Cuts at: 4108 6362 6858 4108 Size: 2254 496 4409 Fragments arranged by size: 4409 2254 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2191 3497 3951 1163 Size: 573 455 1306 454 4371 Fragments arranged by size: 4371 1306 573 455 454 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4609 5370 6398 1540 Size: 275 186 2608 761 1028 2301 Fragments arranged by size: 2608 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3432 3488 3816 5220 5305 6376 3432 Size: 56 328 1404 85 1071 4215 Fragments arranged by size: 4215 1404 1071 328 85 56 BsmI GAATG_Cn' Cuts at: 4670 4763 4670 Size: 93 7066 BsmBI CGTCTCn'nnnn_ Cuts at: 3516 3516 Size: 7159 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2766 3432 3488 3816 4478 5220 5305 Size: 399 976 666 56 328 662 742 85 Cuts at: 5305 6376 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 976 742 666 662 399 328 85 56 BspEI T'CCGG_A Cuts at: 2613 6822 2613 Size: 4209 2950 BspHI T'CATG_A Cuts at: 5048 6778 5048 Size: 1730 5429 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2163 2629 3392 4514 844 Size: 1319 466 763 1122 3489 Fragments arranged by size: 3489 1319 1122 763 466 BsrBI CCG'CTC Cuts at: 1394 1823 5046 1394 Size: 429 3223 3507 Fragments arranged by size: 3507 3223 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4059 5654 5828 65 Size: 1183 2811 1595 174 1396 Fragments arranged by size: 2811 1595 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5803 5990 1700 Size: 110 3993 187 2869 Fragments arranged by size: 3993 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7159 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7159 BssSI C'ACGA_G Cuts at: 3518 5219 6231 3518 Size: 1701 1012 4446 Fragments arranged by size: 4446 1701 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 3427 794 Size: 2633 4526 BstBI TT'CG_AA Cuts at: 2007 5963 2007 Size: 3956 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7159 BstYI r'GATC_y Cuts at: 1972 3087 4396 4489 4856 5241 5258 6710 6796 Size: 1115 1309 93 367 385 17 1452 86 Cuts at: 6796 6808 7154 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1309 1115 385 367 346 93 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3895 3895 Size: 7159 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 6607 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6420 Fragments arranged by size: 6420 683 56 BtsI GCAGTG_nn' Cuts at: 2212 3816 4044 4096 4675 5469 5489 2212 Size: 1604 228 52 579 794 20 3882 Fragments arranged by size: 3882 1604 794 579 228 52 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7159 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3649 3684 529 Size: 35 3085 35 4004 Fragments arranged by size: 4004 3085 35 35 DraI TTT'AAA Cuts at: 4463 4463 Size: 7159 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4160 6166 809 Size: 507 2844 2006 1802 Fragments arranged by size: 2844 2006 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4606 5495 7124 Size: 54 1425 50 275 18 2776 889 1629 Cuts at: 7124 8 Size: 43 Fragments arranged by size: 2776 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4606 1537 Size: 275 2794 4090 Fragments arranged by size: 4090 2794 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2346 3573 3768 4228 5089 1353 Size: 993 1227 195 460 861 3423 Fragments arranged by size: 3423 1227 993 861 460 195 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5746 6120 6266 387 Size: 1064 138 4157 374 146 1280 Fragments arranged by size: 4157 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3486 3486 Size: 7159 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2601 2715 2946 3183 3479 3513 3575 Size: 393 969 114 231 237 296 34 62 Cuts at: 3575 3633 4069 4194 4209 5222 5819 6606 6874 Size: 58 436 125 15 1013 597 787 268 Cuts at: 6874 1239 Size: 1524 Fragments arranged by size: 1524 1013 969 787 597 436 393 296 268 237 231 125 114 62 58 34 15 EcoNI CCTnn'n_nnAGG Cuts at: 2031 2031 Size: 7159 EcoO109I rG'GnC_Cy Cuts at: 2774 3173 3247 2774 Size: 399 74 6686 Fragments arranged by size: 6686 399 74 EcoRI G'AATT_C Cuts at: 4468 4846 4468 Size: 378 6781 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3131 4436 3131 Size: 1305 5854 FspI TGC'GCA Cuts at: 5665 5665 Size: 7159 HaeII r_GCGC'y Cuts at: 1678 2155 6306 1678 Size: 477 4151 2531 Fragments arranged by size: 4151 2531 477 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2800 2832 3657 3689 3764 Size: 32 135 32 1228 32 825 32 75 Cuts at: 3764 3796 4142 4174 5800 5832 5874 5906 1373 Size: 32 346 32 1626 32 42 32 2626 Fragments arranged by size: 2626 1626 1228 825 346 135 75 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3670 4503 4749 669 Size: 988 281 1732 833 246 3079 Fragments arranged by size: 3079 1732 988 833 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7159 HpaI GTT'AAC Cuts at: 4749 4749 Size: 7159 KpnI G_GTAC'C Cuts at: 4484 4484 Size: 7159 MfeI C'AATT_G Cuts at: 4758 4758 Size: 7159 MluI A'CGCG_T Cuts at: 7035 7035 Size: 7159 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4235 6273 6457 1305 Size: 2930 2038 184 2007 Fragments arranged by size: 2930 2038 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 5337 Fragments arranged by size: 5337 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3821 5117 5476 5635 518 Size: 648 2655 1296 359 159 2042 Fragments arranged by size: 2655 2042 1296 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2085 2425 3038 4067 4124 4575 5252 6400 Size: 479 340 613 1029 57 451 677 1148 Cuts at: 6400 6645 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 1029 677 613 479 451 340 245 57 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 6607 NdeI CA'TA_TG Cuts at: 387 387 Size: 7159 NheI G'CTAG_C Cuts at: 1052 2366 1052 Size: 1314 5845 NotI GC'GGCC_GC Cuts at: 4606 4606 Size: 7159 NspI r_CATG'y Cuts at: 1132 1229 2070 3034 3724 4517 6062 1132 Size: 97 841 964 690 793 1545 2229 Fragments arranged by size: 2229 1545 964 841 793 690 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7159 PciI A'CATG_T Cuts at: 2066 6058 2066 Size: 3992 3167 PflMI CCAn_nnn'nTGG Cuts at: 1108 3880 1108 Size: 2772 4387 PmeI GTTT'AAAC Cuts at: 4463 4463 Size: 7159 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5228 5260 6741 6773 5228 Size: 32 1481 32 5614 Fragments arranged by size: 5614 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2774 3173 3247 2774 Size: 399 74 6686 Fragments arranged by size: 6686 399 74 PshAI GACnn'nnGTC Cuts at: 1613 2609 1613 Size: 996 6163 PsiI TTA'TAA Cuts at: 4729 4729 Size: 7159 PstI C_TGCA'G Cuts at: 830 2503 2626 3403 4511 830 Size: 1673 123 777 1108 3478 Fragments arranged by size: 3478 1673 1108 777 123 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7159 PvuII CAG'CTG Cuts at: 2085 2425 3038 4124 2085 Size: 340 613 1086 5120 Fragments arranged by size: 5120 1086 613 340 SacI G_AGCT'C Cuts at: 3488 3488 Size: 7159 SalI G'TCGA_C Cuts at: 1936 4501 1936 Size: 2565 4594 SbfI CC_TGCA'GG Cuts at: 3403 4511 3403 Size: 1108 6051 ScaI AGT'ACT Cuts at: 5407 7119 5407 Size: 1712 5447 SfcI C'TryA_G Cuts at: 826 1045 2499 2622 3399 4097 4507 5642 6323 Size: 219 1454 123 777 698 410 1135 681 Cuts at: 6323 6514 826 Size: 191 1471 Fragments arranged by size: 1471 1454 1135 777 698 681 410 219 191 123 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7159 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7159 SmaI CCC'GGG Cuts at: 1872 4486 1872 Size: 2614 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2672 2997 3014 3161 4427 5263 Size: 197 926 729 325 17 147 1266 836 Cuts at: 5263 6164 6426 820 Size: 901 262 1553 Fragments arranged by size: 1553 1266 926 901 836 729 325 262 197 147 17 SnaBI TAC'GTA Cuts at: 493 4456 4950 493 Size: 3963 494 2702 Fragments arranged by size: 3963 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 7159 SphI G_CATG'C Cuts at: 1132 4517 1132 Size: 3385 3774 SspI AAT'ATT Cuts at: 5 52 5083 5 Size: 47 5031 2081 Fragments arranged by size: 5031 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7159 StyI C'CwwG_G Cuts at: 513 1065 2844 3942 4598 513 Size: 552 1779 1098 656 3074 Fragments arranged by size: 3074 1779 1098 656 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3379 5186 5203 5356 5541 5986 325 Size: 1255 1799 1807 17 153 185 445 1498 Fragments arranged by size: 1807 1799 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 5405 7117 96 Size: 274 80 33 51 1443 3428 1712 138 Fragments arranged by size: 3428 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2586 2618 7119 7151 2586 Size: 32 4501 32 2594 Fragments arranged by size: 4501 2594 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7159 XbaI T'CTAG_A Cuts at: 4495 4495 Size: 7159 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7159 XmaI C'CCGG_G Cuts at: 1870 4484 1870 Size: 2614 4545 XmnI GAAnn'nnTTC Cuts at: 2460 2887 3111 3114 5288 2460 Size: 427 224 3 2174 4331 Fragments arranged by size: 4331 2174 427 224 3 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4967 276 Size: 53 83 186 936 3433 2468 Fragments arranged by size: 3433 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BstXI BstZ17I DraIII EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI