Restriction map for
pFN21ASDA0639
(Circular) (Six-base) MAPSORT of: pFN21ASDA0639.seq Check: 7761 from: 1 to: 10207
pFN21ASDA0639 10207 bp
With 182 enzymes: SgfI *
January 16, 2009 15:30 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 8017 278
Size: 53 83 186 936 6481 2468
Fragments arranged by size:
6481 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2571 3741 5173 7550 1937
Size: 634 1170 1432 2377 4594
Fragments arranged by size:
4594 2377 1432 1170 634
Acc65I G'GTAC_C
Cuts at: 7528 7528
Size: 10207
AclI AA'CG_TT
Cuts at: 1563 4055 8334 8707 1563
Size: 2492 4279 373 3063
Fragments arranged by size:
4279 3063 2492 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2852 3314 3625 4758 7261 8270 9654 9922
Size: 1220 462 311 1133 2503 1009 1384 268
Cuts at: 9922 1632
Size: 1917
Fragments arranged by size:
2503 1917 1384 1220 1133 1009 462 311
268
AfeI AGC'GCT
Cuts at: 4012 4012
Size: 10207
AflII C'TTAA_G
Cuts at: 820 1017 4024 820
Size: 197 3007 7003
Fragments arranged by size:
7003 3007 197
AflIII A'CryG_T
Cuts at: 1203 4946 9106 10083 1203
Size: 3743 4160 977 1327
Fragments arranged by size:
4160 3743 1327 977
AgeI A'CCGG_T
Cuts at: 9038 9038
Size: 10207
AhdI GACnn_n'nnGTC
Cuts at: 2567 3514 4950 8936 2567
Size: 947 1436 3986 3838
Fragments arranged by size:
3986 3838 1436 947
AleI CACnn'nnGTG
Cuts at: 1166 5747 1166
Size: 4581 5626
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 6515 1184
Size: 5331 4876
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6148 6180 7472 7504 6148
Size: 32 1292 32 8851
Fragments arranged by size:
8851 1292 32 32
AlwNI CAG_nnn'CTG
Cuts at: 2654 2816 4672 4704 5413 5513 5600 6020 7081
Size: 162 1856 32 709 100 87 420 1061
Cuts at: 7081 7950 9522 2654
Size: 869 1572 3339
Fragments arranged by size:
3339 1856 1572 1061 869 709 420 162
100 87 32
ApaI G_GGCC'C
Cuts at: 5491 6563 6592 6693 6773 7340 5491
Size: 1072 29 101 80 567 8358
Fragments arranged by size:
8358 1072 567 101 80 29
ApaLI G'TGCA_C
Cuts at: 8264 9420 8264
Size: 1156 9051
ApoI r'AATT_y
Cuts at: 1493 2478 2924 3451 3483 4991 7516 7742 7894
Size: 985 446 527 32 1508 2525 226 152
Cuts at: 7894 10003 1493
Size: 2109 1697
Fragments arranged by size:
2525 2109 1697 1508 985 527 446 226
152 32
AseI AT'TA_AT
Cuts at: 160 1032 2387 8761 160
Size: 872 1355 6374 1606
Fragments arranged by size:
6374 1606 1355 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 10207
AvaI C'yCGr_G
Cuts at: 1870 1943 2169 2975 4442 6830 6932 7033 7060
Size: 73 226 806 1467 2388 102 101 27
Cuts at: 7060 7379 7405 7532 1870
Size: 319 26 127 4545
Fragments arranged by size:
4545 2388 1467 806 319 226 127 102
101 73 27 26
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 5493 5526 5493
Size: 33 10174
BamHI G'GATC_C
Cuts at: 3697 7537 7904 3697
Size: 3840 367 6000
Fragments arranged by size:
6000 3840 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 6835 6943 7064 7151 7373
Size: 325 217 627 5048 108 121 87 222
Cuts at: 7373 7528 8983 618
Size: 155 1455 1842
Fragments arranged by size:
5048 1842 1455 627 325 222 217 155
121 108 87
BanII G_rGCy'C
Cuts at: 1391 5221 5491 6563 6592 6693 6773 6831 6885
Size: 3830 270 1072 29 101 80 58 54
Cuts at: 6885 6911 7227 7340 7412 7526 1391
Size: 26 316 113 72 114 4072
Fragments arranged by size:
4072 3830 1072 316 270 114 113 101
80 72 58 54 29 26
BbeI G_GCGC'C
Cuts at: 6839 6947 7068 7155 6839
Size: 108 121 87 9891
Fragments arranged by size:
9891 121 108 87
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2813 3327 4429 4836 4946 5262 928
Size: 971 914 514 1102 407 110 316 5873
Fragments arranged by size:
5873 1102 971 914 514 407 316 110
BbvCI CC'TCA_GC
Cuts at: 4038 4079 4134 5727 4038
Size: 41 55 1593 8518
Fragments arranged by size:
8518 1593 55 41
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 6599 6633 7031 7065 7195 7229 8398
Size: 34 4662 34 398 34 130 34 1169
Cuts at: 8398 8432 1903
Size: 34 3678
Fragments arranged by size:
4662 3678 1169 398 130 34 34 34
34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2049 6274 8099 9309 2049
Size: 4225 1825 1210 2947
Fragments arranged by size:
4225 2947 1825 1210
BclI T'GATC_A
Cuts at: 1548 2197 4795 1548
Size: 649 2598 6960
Fragments arranged by size:
6960 2598 649
BfrBI ATG'CAT
Cuts at: 2312 5315 2312
Size: 3003 7204
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3002 6821 7382
Size: 107 122 71 631 605 1330 3819 561
Cuts at: 7382 8818 136
Size: 1436 1525
Fragments arranged by size:
3819 1525 1436 1330 631 605 561 122
107 71
BglII A'GATC_T
Cuts at: 6202 6603 10202 6202
Size: 401 3599 6207
Fragments arranged by size:
6207 3599 401
BlpI GC'TnA_GC
Cuts at: 4014 5732 5792 6546 7624 4014
Size: 1718 60 754 1078 6597
Fragments arranged by size:
6597 1718 1078 754 60
Bme1580I G_kGCm'C
Cuts at: 1790 2369 5491 5744 6489 6563 6592 6693 6773
Size: 579 3122 253 745 74 29 101 80
Cuts at: 6773 7208 7340 7378 8268 9424 1790
Size: 435 132 38 890 1156 2573
Fragments arranged by size:
3122 2573 1156 890 745 579 435 253
132 101 80 74 38 29
BmgBI CAC'GTC
Cuts at: 1318 3516 6568 1318
Size: 2198 3052 4957
Fragments arranged by size:
4957 3052 2198
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4349 4665 5501
Size: 416 53 83 399 24 2932 316 836
Cuts at: 5501 5634 7938 8896 442
Size: 133 2304 958 1753
Fragments arranged by size:
2932 2304 1753 958 836 416 399 316
133 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 10207
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 7009 7009
Size: 10207
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2769 3177 3568 3739 3920 4171 5435 6321
Size: 1530 408 391 171 181 251 1264 886
Cuts at: 6321 8867 1239
Size: 2546 2579
Fragments arranged by size:
2579 2546 1530 1264 886 408 391 251
181 171
Bpu10I CC'TnA_GC
Cuts at: 4038 4079 4134 5727 4038
Size: 41 55 1593 8518
Fragments arranged by size:
8518 1593 55 41
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3329 6401 8332 9197 9495 3329
Size: 3072 1931 865 298 4041
Fragments arranged by size:
4041 3072 1931 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2493 4308 4597 4601 5228 8870 882
Size: 617 994 1815 289 4 627 3642 2219
Fragments arranged by size:
3642 2219 1815 994 627 617 289 4
BsaAI yAC'GTr
Cuts at: 493 1204 4525 4949 7180 7209 7504 7998 493
Size: 711 3321 424 2231 29 295 494 2702
Fragments arranged by size:
3321 2702 2231 711 494 424 295 29
BsaBI GATnn'nnATC
Cuts at: 10201 10201
Size: 10207
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 6762 6836 6944 7065
Size: 53 83 186 936 5229 74 108 121
Cuts at: 7065 7152 8014 8396 9031 275
Size: 87 862 382 635 1451
Fragments arranged by size:
5229 1451 936 862 635 382 186 121
108 87 83 74 53
BsaWI w'CCGG_w
Cuts at: 3942 6673 6966 7482 8640 9038 9312 9459 9870
Size: 2731 293 516 1158 398 274 147 411
Cuts at: 9870 3942
Size: 4279
Fragments arranged by size:
4279 2731 1158 516 411 398 293 274
147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2742 2772 3734 3764 4736
Size: 30 165 30 1339 30 962 30 972
Cuts at: 4736 4766 5408 5438 5859 5889 6148 6178 6655
Size: 30 642 30 421 30 259 30 477
Cuts at: 6655 6685 6725 6755 1178
Size: 30 40 30 4630
Fragments arranged by size:
4630 1339 972 962 642 477 421 259
165 40 30 30 30 30 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2687 3272 3293 3531 3742 4055 4151 4741
Size: 1501 585 21 238 211 313 96 590
Cuts at: 4741 4744 5453 5654 6169 6404 6554 6893 1186
Size: 3 709 201 515 235 150 339 4500
Fragments arranged by size:
4500 1501 709 590 585 515 339 313
238 235 211 201 150 96 21 3
BseYI C'CCAG_C
Cuts at: 3391 4007 5491 6013 6695 6894 7013 7313 7369
Size: 616 1484 522 682 199 119 300 56
Cuts at: 7369 9410 9906 3391
Size: 2041 496 3692
Fragments arranged by size:
3692 2041 1484 682 616 522 496 300
199 119 56
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3879 4505 5156 6521 6596 1163
Size: 573 2143 626 651 1365 75 4774
Fragments arranged by size:
4774 2143 1365 651 626 573 75
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5854 6461 6867 6916 7657 8418
Size: 275 186 3853 607 406 49 741 761
Cuts at: 8418 9446 1540
Size: 1028 2301
Fragments arranged by size:
3853 2301 1028 761 741 607 406 275
186 49
BsiHKAI G_wGCw'C
Cuts at: 3927 7227 8268 8353 9424 3927
Size: 3300 1041 85 1071 4710
Fragments arranged by size:
4710 3300 1071 1041 85
BsmI GAATG_Cn'
Cuts at: 2296 3388 7054 7718 7811 2296
Size: 1092 3666 664 93 4692
Fragments arranged by size:
4692 3666 1092 664 93
BsmBI CGTCTCn'nnnn_
Cuts at: 6468 6745 6468
Size: 277 9930
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2369 3927 5221 5491 5744 6489 6563
Size: 399 579 1558 1294 270 253 745 74
Cuts at: 6563 6592 6693 6773 6831 6885 6911 7208 7227
Size: 29 101 80 58 54 26 297 19
Cuts at: 7227 7340 7378 7412 7526 8268 8353 9424 1391
Size: 113 38 34 114 742 85 1071 2174
Fragments arranged by size:
2174 1558 1294 1071 745 742 579 399
297 270 253 114 113 101 85 80
74 58 54 38 34 29 26 19
BspEI T'CCGG_A
Cuts at: 3942 6673 6966 9870 3942
Size: 2731 293 2904 4279
Fragments arranged by size:
4279 2904 2731 293
BspHI T'CATG_A
Cuts at: 8096 9826 8096
Size: 1730 8477
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2234 4502 6625 7239 7562 844
Size: 1390 2268 2123 614 323 3489
Fragments arranged by size:
3489 2268 2123 1390 614 323
BsrBI CCG'CTC
Cuts at: 1394 1823 5212 5758 8094 1394
Size: 429 3389 546 2336 3507
Fragments arranged by size:
3507 3389 2336 546 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3189 3838 4128 4798 4810 8702 8876
Size: 1183 1941 649 290 670 12 3892 174
Cuts at: 8876 65
Size: 1396
Fragments arranged by size:
3892 1941 1396 1183 670 649 290 174
12
BsrFI r'CCGG_y
Cuts at: 1700 1810 2070 3207 4720 6240 7054 8851 9038
Size: 110 260 1137 1513 1520 814 1797 187
Cuts at: 9038 1700
Size: 2869
Fragments arranged by size:
2869 1797 1520 1513 1137 814 260 187
110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 10207
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 10207
BssSI C'ACGA_G
Cuts at: 6902 8267 9279 6902
Size: 1365 1012 7830
Fragments arranged by size:
7830 1365 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 10207
BstBI TT'CG_AA
Cuts at: 2007 9011 2007
Size: 7004 3203
BstEII G'GTnAC_C
Cuts at: 1184 3808 6526 6661 1184
Size: 2624 2718 135 4730
Fragments arranged by size:
4730 2718 2624 135
BstXI CCAn_nnnn'nTGG
Cuts at: 2101 4108 4987 5499 7423 2101
Size: 2007 879 512 1924 4885
Fragments arranged by size:
4885 2007 1924 879 512
BstYI r'GATC_y
Cuts at: 1972 3130 3697 6202 6340 6603 7537 7904 8289
Size: 1158 567 2505 138 263 934 367 385
Cuts at: 8289 8306 9758 9844 9856 10202 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
2505 1977 1452 1158 934 567 385 367
346 263 138 86 17 12
BstZ17I GTA'TAC
Cuts at: 5174 5174
Size: 10207
Bsu36I CC'TnA_GG
Cuts at: 6053 6819 7237 6053
Size: 766 418 9023
Fragments arranged by size:
9023 766 418
BtgI C'CryG_G
Cuts at: 513 1065 2022 2997 3604 5709 5748 6861 7146
Size: 552 957 975 607 2105 39 1113 285
Cuts at: 7146 7332 513
Size: 186 3388
Fragments arranged by size:
3388 2105 1113 975 957 607 552 285
186 39
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 4590 6453 487
Size: 683 56 3364 1863 4241
Fragments arranged by size:
4241 3364 1863 683 56
BtsI GCAGTG_nn'
Cuts at: 2410 2797 4777 5479 6120 7723 8517 8537 2410
Size: 387 1980 702 641 1603 794 20 4080
Fragments arranged by size:
4080 1980 1603 794 702 641 387 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 10207
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 10172
DraI TTT'AAA
Cuts at: 2455 3423 7511 2455
Size: 968 4088 5151
Fragments arranged by size:
5151 4088 968
DraIII CAC_nnn'GTG
Cuts at: 3094 4684 3094
Size: 1590 8617
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 9214 809
Size: 507 7898 1802
Fragments arranged by size:
7898 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4428 5851 6458
Size: 54 1425 50 275 18 2598 1423 607
Cuts at: 6458 6864 7446 7654 8543 10172 8
Size: 406 582 208 889 1629 43
Fragments arranged by size:
2598 1629 1425 1423 889 607 582 406
275 208 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5851 6458 6864 7654 1537
Size: 275 4039 607 406 790 4090
Fragments arranged by size:
4090 4039 790 607 406 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3333 4224 4762 5244 5631 6440 8137 1353
Size: 1980 891 538 482 387 809 1697 3423
Fragments arranged by size:
3423 1980 1697 891 809 538 482 387
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 7402 8794 9168 9314 387
Size: 1064 138 5813 1392 374 146 1280
Fragments arranged by size:
5813 1392 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 7225 7225
Size: 10207
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2769 2852 3177 3314 3568 3625 3739
Size: 393 1137 83 325 137 254 57 114
Cuts at: 3739 3920 4171 4758 5435 6321 7261 8270 8867
Size: 181 251 587 677 886 940 1009 597
Cuts at: 8867 9654 9922 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1137 1009 940 886 787 677 597
587 393 325 268 254 251 181 137
114 83 57
EcoNI CCTnn'n_nnAGG
Cuts at: 4322 5435 7448 4322
Size: 1113 2013 7081
Fragments arranged by size:
7081 2013 1113
EcoO109I rG'GnC_Cy
Cuts at: 2561 3106 3820 5487 5488 6560 6588 6589 6690
Size: 545 714 1667 1 1072 28 1 101
Cuts at: 6690 6770 6937 7018 7078 7111 7184 7337 2561
Size: 80 167 81 60 33 73 153 5431
Fragments arranged by size:
5431 1667 1072 714 545 167 153 101
81 80 73 60 33 28 1 1
EcoRI G'AATT_C
Cuts at: 3483 4991 7516 7894 3483
Size: 1508 2525 378 5796
Fragments arranged by size:
5796 2525 1508 378
EcoRV GAT'ATC
Cuts at: 3463 3463
Size: 10207
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 5426 6442 7291 5426
Size: 1016 849 8342
Fragments arranged by size:
8342 1016 849
FspI TGC'GCA
Cuts at: 8713 8713
Size: 10207
HaeII r_GCGC'y
Cuts at: 1678 2817 3907 4014 6839 6925 6947 7068 7155
Size: 1139 1090 107 2825 86 22 121 87
Cuts at: 7155 7398 9354 1678
Size: 243 1956 2531
Fragments arranged by size:
2825 2531 1956 1139 1090 243 121 107
87 86 22
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2265 2297 2553 2585 2600
Size: 32 135 32 693 32 256 32 15
Cuts at: 2600 2632 2720 2752 3500 3532 3734 3766 4717
Size: 32 88 32 748 32 202 32 951
Cuts at: 4717 4736 4749 4768 4936 4968 5103 5135 5555
Size: 19 13 19 168 32 135 32 420
Cuts at: 5555 5587 5878 5910 6076 6108 6189 6221 6898
Size: 32 291 32 166 32 81 32 677
Cuts at: 6898 6930 7220 7252 8848 8880 8922 8954 1373
Size: 32 290 32 1596 32 42 32 2626
Fragments arranged by size:
2626 1596 951 748 693 677 420 291
290 256 202 168 166 135 135 88
81 42 32 32 32 32 32 32
32 32 32 32 32 32 32 32
32 32 32 32 19 19 15 13
HincII GTy'rAC
Cuts at: 669 1657 1938 2572 3778 4819 5427 6129 7551
Size: 988 281 634 1206 1041 608 702 1422
Cuts at: 7551 7797 669
Size: 246 3079
Fragments arranged by size:
3079 1422 1206 1041 988 702 634 608
281 246
HindIII A'AGCT_T
Cuts at: 748 5357 748
Size: 4609 5598
HpaI GTT'AAC
Cuts at: 3778 4819 6129 7797 3778
Size: 1041 1310 1668 6188
Fragments arranged by size:
6188 1668 1310 1041
KasI G'GCGC_C
Cuts at: 6835 6943 7064 7151 6835
Size: 108 121 87 9891
Fragments arranged by size:
9891 121 108 87
KpnI G_GTAC'C
Cuts at: 7532 7532
Size: 10207
MfeI C'AATT_G
Cuts at: 4866 5348 7806 4866
Size: 482 2458 7267
Fragments arranged by size:
7267 2458 482
MluI A'CGCG_T
Cuts at: 10083 10083
Size: 10207
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3490 4284 6738 9321 9505 1305
Size: 2185 794 2454 2583 184 2007
Fragments arranged by size:
2583 2454 2185 2007 794 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 4430 7448 10
Size: 54 1425 343 2598 3018 2769
Fragments arranged by size:
3018 2769 2598 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4424 4803 5747 6497 6524 8165 8524
Size: 648 3258 379 944 750 27 1641 359
Cuts at: 8524 8683 518
Size: 159 2042
Fragments arranged by size:
3258 2042 1641 944 750 648 379 359
159 27
MspA1I CmG'CkG
Cuts at: 1606 2372 3023 3166 3733 5711 6659 7439 7623
Size: 766 651 143 567 1978 948 780 184
Cuts at: 7623 8300 9448 9693 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1978 1148 948 780 766 677 651
567 245 184 143
NaeI GCC'GGC
Cuts at: 7056 7056
Size: 10207
NarI GG'CG_CC
Cuts at: 6836 6944 7065 7152 6836
Size: 108 121 87 9891
Fragments arranged by size:
9891 121 108 87
NcoI C'CATG_G
Cuts at: 513 1065 2022 2997 3604 513
Size: 552 957 975 607 7116
Fragments arranged by size:
7116 975 957 607 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 10207
NgoMIV G'CCGG_C
Cuts at: 7054 7054
Size: 10207
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 10207
NotI GC'GGCC_GC
Cuts at: 7654 7654
Size: 10207
NsiI A_TGCA'T
Cuts at: 2314 5317 2314
Size: 3003 7204
NspI r_CATG'y
Cuts at: 1132 1229 2316 5812 7009 7565 9110 1132
Size: 97 1087 3496 1197 556 1545 2229
Fragments arranged by size:
3496 2229 1545 1197 1087 556 97
PasI CC'CwG_GG
Cuts at: 1348 3823 5959 6766 1348
Size: 2475 2136 807 4789
Fragments arranged by size:
4789 2475 2136 807
PciI A'CATG_T
Cuts at: 9106 9106
Size: 10207
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3094 5513 5600 5965 1108
Size: 1986 2419 87 365 5350
Fragments arranged by size:
5350 2419 1986 365 87
PfoI T'CCnGG_A
Cuts at: 3126 3135 3156 3234 5105 5544 6343 7113 3126
Size: 9 21 78 1871 439 799 770 6220
Fragments arranged by size:
6220 1871 799 770 439 78 21 9
PmeI GTTT'AAAC
Cuts at: 7511 7511
Size: 10207
PmlI CAC'GTG
Cuts at: 4525 7209 4525
Size: 2684 7523
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5747 5779 6148 6180 7121 7153 8276 8308 9789
Size: 32 369 32 941 32 1123 32 1481
Cuts at: 9789 9821 5747
Size: 32 6133
Fragments arranged by size:
6133 1481 1123 941 369 32 32 32
32 32
PpuMI rG'GwC_Cy
Cuts at: 2561 3106 7018 7111 2561
Size: 545 3912 93 5657
Fragments arranged by size:
5657 3912 545 93
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 10207
PsiI TTA'TAA
Cuts at: 2484 7777 2484
Size: 5293 4914
PspOMI G'GGCC_C
Cuts at: 5487 6559 6588 6689 6769 7336 5487
Size: 1072 29 101 80 567 8358
Fragments arranged by size:
8358 1072 567 101 80 29
PspXI vC'TCGA_Gb
Cuts at: 4442 6932 4442
Size: 2490 7717
PstI C_TGCA'G
Cuts at: 830 2289 2821 3022 7559 830
Size: 1459 532 201 4537 3478
Fragments arranged by size:
4537 3478 1459 532 201
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 10207
PvuII CAG'CTG
Cuts at: 2372 3023 3166 3733 7439 2372
Size: 651 143 567 3706 5140
Fragments arranged by size:
5140 3706 651 567 143
SacI G_AGCT'C
Cuts at: 7227 7227
Size: 10207
SacII CC_GC'GG
Cuts at: 5712 5712
Size: 10207
SalI G'TCGA_C
Cuts at: 1936 2570 7549 1936
Size: 634 4979 4594
Fragments arranged by size:
4979 4594 634
SanDI GG'GwC_CC
Cuts at: 7018 7018
Size: 10207
SbfI CC_TGCA'GG
Cuts at: 7559 7559
Size: 10207
ScaI AGT'ACT
Cuts at: 5336 7274 8455 10167 5336
Size: 1938 1181 1712 5376
Fragments arranged by size:
5376 1938 1712 1181
SfcI C'TryA_G
Cuts at: 826 1045 2285 2817 3018 4172 5040 6069 7555
Size: 219 1240 532 201 1154 868 1029 1486
Cuts at: 7555 8690 9371 9562 826
Size: 1135 681 191 1471
Fragments arranged by size:
1486 1471 1240 1154 1135 1029 868 681
532 219 201 191
SfoI GGC'GCC
Cuts at: 6837 6945 7066 7153 6837
Size: 108 121 87 9891
Fragments arranged by size:
9891 121 108 87
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 10207
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 10207
SmaI CCC'GGG
Cuts at: 1872 2171 7035 7062 7534 1872
Size: 299 4864 27 472 4545
Fragments arranged by size:
4864 4545 472 299 27
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3308 4024 4442 6416 6932 8311
Size: 197 926 1365 716 418 1974 516 1379
Cuts at: 8311 9212 9474 820
Size: 901 262 1553
Fragments arranged by size:
1974 1553 1379 1365 926 901 716 516
418 262 197
SnaBI TAC'GTA
Cuts at: 493 7504 7998 493
Size: 7011 494 2702
Fragments arranged by size:
7011 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 10207
SphI G_CATG'C
Cuts at: 1132 7009 7565 1132
Size: 5877 556 3774
Fragments arranged by size:
5877 3774 556
SspI AAT'ATT
Cuts at: 5 52 5944 8131 5
Size: 47 5892 2187 2081
Fragments arranged by size:
5892 2187 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 10207
StyI C'CwwG_G
Cuts at: 513 1065 2022 2269 2997 3604 7449 7646 513
Size: 552 957 247 728 607 3845 197 3074
Fragments arranged by size:
3845 3074 957 728 607 552 247 197
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2861 7361 8234 8251 8404 8589 9034
Size: 1255 1281 4500 873 17 153 185 445
Cuts at: 9034 325
Size: 1498
Fragments arranged by size:
4500 1498 1281 1255 873 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3414 5334 7272
Size: 274 80 33 51 1443 1437 1920 1938
Cuts at: 7272 8453 10165 96
Size: 1181 1712 138
Fragments arranged by size:
1938 1920 1712 1443 1437 1181 274 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2742 2774 4324 4356 5859 5891 6655 6687 6725
Size: 32 1550 32 1503 32 764 32 38
Cuts at: 6725 6757 10167 10199 2742
Size: 32 3410 32 2750
Fragments arranged by size:
3410 2750 1550 1503 764 38 32 32
32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 6754 7109 1316
Size: 5438 355 4414
Fragments arranged by size:
5438 4414 355
XbaI T'CTAG_A
Cuts at: 7543 7543
Size: 10207
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2041 2101 3820 2041
Size: 60 1719 8428
Fragments arranged by size:
8428 1719 60
XhoI C'TCGA_G
Cuts at: 1943 4442 6932 1943
Size: 2499 2490 5218
Fragments arranged by size:
5218 2499 2490
XmaI C'CCGG_G
Cuts at: 1870 2169 7033 7060 7532 1870
Size: 299 4864 27 472 4545
Fragments arranged by size:
4864 4545 472 299 27
XmnI GAAnn'nnTTC
Cuts at: 2493 8336 2493
Size: 5843 4364
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 8015 276
Size: 53 83 186 936 6481 2468
Fragments arranged by size:
6481 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI
BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI
NsiI NspI PasI PciI PflMI PfoI PmeI PmlI
PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI
PvuII SacI SacII SalI SanDI SbfI ScaI SfcI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AscI AvrII BsiWI FseI FspAI NruI PacI
PsrI RsrII SapI SexAI SfiI SrfI SwaI