(Circular) (Six-base) MAPSORT of: pFN21ASDA0634.seq Check: 8235 from: 1 to: 8593 pFN21ASDA0634 8593 bp With 182 enzymes: SgfI * January 16, 2009 15:30 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3877 4622 5001 3877 Size: 745 379 7469 Fragments arranged by size: 7469 745 379 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3071 6403 278 Size: 53 83 186 936 1535 3332 2468 Fragments arranged by size: 3332 2468 1535 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3623 5936 1937 Size: 1686 2313 4594 Fragments arranged by size: 4594 2313 1686 Acc65I G'GTAC_C Cuts at: 5914 5914 Size: 8593 AclI AA'CG_TT Cuts at: 1563 6720 7093 1563 Size: 5157 373 3063 Fragments arranged by size: 5157 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2237 5331 6656 8040 8308 1632 Size: 605 3094 1325 1384 268 1917 Fragments arranged by size: 3094 1917 1384 1325 605 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 8396 AflIII A'CryG_T Cuts at: 1203 5828 5862 7492 8469 1203 Size: 4625 34 1630 977 1327 Fragments arranged by size: 4625 1630 1327 977 34 AgeI A'CCGG_T Cuts at: 7424 7424 Size: 8593 AhdI GACnn_n'nnGTC Cuts at: 3805 5436 7322 3805 Size: 1631 1886 5076 Fragments arranged by size: 5076 1886 1631 AleI CACnn'nnGTG Cuts at: 1166 3513 4874 1166 Size: 2347 1361 4885 Fragments arranged by size: 4885 2347 1361 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2435 3164 1184 Size: 1251 729 6613 Fragments arranged by size: 6613 1251 729 AlwNI CAG_nnn'CTG Cuts at: 2518 3528 4027 4654 4759 5210 6336 7908 2518 Size: 1010 499 627 105 451 1126 1572 3203 Fragments arranged by size: 3203 1572 1126 1010 627 499 451 105 ApaI G_GGCC'C Cuts at: 4148 5649 4148 Size: 1501 7092 ApaLI G'TGCA_C Cuts at: 2498 4953 6650 7806 2498 Size: 2455 1697 1156 3285 Fragments arranged by size: 3285 2455 1697 1156 ApoI r'AATT_y Cuts at: 1493 4172 5228 5902 6128 6280 8389 1493 Size: 2679 1056 674 226 152 2109 1697 Fragments arranged by size: 2679 2109 1697 1056 674 226 152 AseI AT'TA_AT Cuts at: 160 1032 7147 160 Size: 872 6115 1606 Fragments arranged by size: 6115 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8593 AvaI C'yCGr_G Cuts at: 1870 1943 2062 2098 2335 3272 3898 5918 1870 Size: 73 119 36 237 937 626 2020 4545 Fragments arranged by size: 4545 2020 937 626 237 119 73 36 AvrII C'CTAG_G Cuts at: 2927 2927 Size: 8593 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 5494 5527 5494 Size: 33 8560 BamHI G'GATC_C Cuts at: 5923 6290 5923 Size: 367 8226 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2036 2171 2276 2357 2528 Size: 325 217 627 249 135 105 81 171 Cuts at: 2528 4900 5914 7369 618 Size: 2372 1014 1455 1842 Fragments arranged by size: 2372 1842 1455 1014 627 325 249 217 171 135 105 81 BanII G_rGCy'C Cuts at: 1391 2070 2199 2342 2604 3077 4148 4365 4611 Size: 679 129 143 262 473 1071 217 246 Cuts at: 4611 4707 4868 5046 5103 5352 5649 5912 1391 Size: 96 161 178 57 249 297 263 4072 Fragments arranged by size: 4072 1071 679 473 297 263 262 249 246 217 178 161 143 129 96 57 BbeI G_GCGC'C Cuts at: 2040 2175 2280 2040 Size: 135 105 8353 Fragments arranged by size: 8353 135 105 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2225 3747 3801 4240 928 Size: 971 326 1522 54 439 5281 Fragments arranged by size: 5281 1522 971 439 326 54 BbvCI CC'TCA_GC Cuts at: 5021 5021 Size: 8593 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2711 2745 6784 6818 1903 Size: 34 774 34 4039 34 3678 Fragments arranged by size: 4039 3678 774 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5380 6485 7695 5380 Size: 1105 1210 6278 Fragments arranged by size: 6278 1210 1105 BclI T'GATC_A Cuts at: 1548 1548 Size: 8593 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2929 4848 7204 Size: 107 122 71 631 605 1257 1919 2356 Cuts at: 7204 136 Size: 1525 Fragments arranged by size: 2356 1919 1525 1257 631 605 122 107 71 BglII A'GATC_T Cuts at: 5481 8588 5481 Size: 3107 5486 BlpI GC'TnA_GC Cuts at: 2045 4472 4862 5411 6010 2045 Size: 2427 390 549 599 4628 Fragments arranged by size: 4628 2427 599 549 390 Bme1580I G_kGCm'C Cuts at: 1790 2502 2871 3273 3914 4148 4957 5270 5649 Size: 712 369 402 641 234 809 313 379 Cuts at: 5649 5751 6654 7810 1790 Size: 102 903 1156 2573 Fragments arranged by size: 2573 1156 903 809 712 641 402 379 369 313 234 102 BmgBI CAC'GTC Cuts at: 1318 2545 1318 Size: 1227 7366 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3155 3538 6324 Size: 416 53 83 399 24 1738 383 2786 Cuts at: 6324 7282 442 Size: 958 1753 Fragments arranged by size: 2786 1753 1738 958 416 399 383 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 8593 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2290 2483 2524 2540 2579 4410 4646 5089 Size: 1051 193 41 16 39 1831 236 443 Cuts at: 5089 5366 5644 5686 5699 7253 1239 Size: 277 278 42 13 1554 2579 Fragments arranged by size: 2579 1831 1554 1051 443 278 277 236 193 42 41 39 16 13 Bpu10I CC'TnA_GC Cuts at: 4978 5021 4978 Size: 43 8550 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3587 4496 4691 5795 6718 7583 7881 3587 Size: 909 195 1104 923 865 298 4299 Fragments arranged by size: 4299 1104 923 909 865 298 195 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4670 5122 5167 5363 5557 7256 882 Size: 617 3171 452 45 196 194 1699 2219 Fragments arranged by size: 3171 2219 1699 617 452 196 194 45 BsaAI yAC'GTr Cuts at: 493 1204 5829 5865 5890 6384 493 Size: 711 4625 36 25 494 2702 Fragments arranged by size: 4625 2702 711 494 36 25 BsaBI GATnn'nnATC Cuts at: 2630 8587 2630 Size: 5957 2636 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2037 2172 2277 3068 Size: 53 83 186 936 504 135 105 791 Cuts at: 3068 6400 6782 7417 275 Size: 3332 382 635 1451 Fragments arranged by size: 3332 1451 936 791 635 504 382 186 135 105 83 53 BsaWI w'CCGG_w Cuts at: 3458 4218 4369 4446 7026 7424 7698 7845 8256 Size: 760 151 77 2580 398 274 147 411 Cuts at: 8256 3458 Size: 3795 Fragments arranged by size: 3795 2580 760 411 398 274 151 147 77 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2930 2960 3944 3974 4325 Size: 30 165 30 1527 30 984 30 351 Cuts at: 4325 4355 4535 4556 4565 4586 4694 4724 1178 Size: 30 180 21 9 21 108 30 5047 Fragments arranged by size: 5047 1527 984 351 180 165 108 30 30 30 30 30 30 21 21 9 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2633 2924 2933 3932 4100 4349 4556 4817 Size: 1447 291 9 999 168 249 207 261 Cuts at: 4817 5786 5849 1186 Size: 969 63 3930 Fragments arranged by size: 3930 1447 999 969 291 261 249 207 168 63 9 BseYI C'CCAG_C Cuts at: 2297 2709 2800 4224 4293 4631 4687 5641 7796 Size: 412 91 1424 69 338 56 954 2155 Cuts at: 7796 8292 2297 Size: 496 2598 Fragments arranged by size: 2598 2155 1424 954 496 412 338 91 69 56 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2441 3487 3838 4451 5029 1163 Size: 573 705 1046 351 613 578 4727 Fragments arranged by size: 4727 1046 705 613 578 573 351 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2081 6043 6804 7832 1540 Size: 275 186 80 3962 761 1028 2301 Fragments arranged by size: 3962 2301 1028 761 275 186 80 BsiHKAI G_wGCw'C Cuts at: 2502 3279 3732 4058 4611 4707 4957 4979 6654 Size: 777 453 326 553 96 250 22 1675 Cuts at: 6654 6739 7810 2502 Size: 85 1071 3285 Fragments arranged by size: 3285 1675 1071 777 553 453 326 250 96 85 22 BsiWI C'GTAC_G Cuts at: 5865 5865 Size: 8593 BsmI GAATG_Cn' Cuts at: 6104 6197 6104 Size: 93 8500 BsmBI CGTCTCn'nnnn_ Cuts at: 2551 2551 Size: 8593 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2070 2199 2342 2502 2604 2871 3077 Size: 399 280 129 143 160 102 267 206 Cuts at: 3077 3273 3279 3732 3914 4058 4148 4365 4611 Size: 196 6 453 182 144 90 217 246 Cuts at: 4611 4707 4868 4957 4979 5046 5103 5270 5352 Size: 96 161 89 22 67 57 167 82 Cuts at: 5352 5649 5751 5912 6654 6739 7810 1391 Size: 297 102 161 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 453 399 297 280 267 246 217 206 196 182 167 161 161 160 144 143 129 102 102 96 90 89 85 82 67 57 22 6 BspEI T'CCGG_A Cuts at: 4369 4446 8256 4369 Size: 77 3810 4706 Fragments arranged by size: 4706 3810 77 BspHI T'CATG_A Cuts at: 2770 3475 6482 8212 2770 Size: 705 3007 1730 3151 Fragments arranged by size: 3151 3007 1730 705 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3810 3877 4622 5001 5948 844 Size: 2966 67 745 379 947 3489 Fragments arranged by size: 3489 2966 947 745 379 67 BsrBI CCG'CTC Cuts at: 1394 1823 2274 6480 1394 Size: 429 451 4206 3507 Fragments arranged by size: 4206 3507 451 429 BsrDI GCAATG_nn' Cuts at: 65 1248 7088 7262 65 Size: 1183 5840 174 1396 Fragments arranged by size: 5840 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2033 2168 2211 2354 3882 4327 4842 Size: 110 223 135 43 143 1528 445 515 Cuts at: 4842 5071 5883 7237 7424 1700 Size: 229 812 1354 187 2869 Fragments arranged by size: 2869 1528 1354 812 515 445 229 223 187 143 135 110 43 BsrGI T'GTAC_A Cuts at: 96 5217 5493 96 Size: 5121 276 3196 Fragments arranged by size: 5121 3196 276 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 8593 BssSI C'ACGA_G Cuts at: 5391 6653 7665 5391 Size: 1262 1012 6319 Fragments arranged by size: 6319 1262 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 3975 4027 5074 794 Size: 3181 52 1047 4313 Fragments arranged by size: 4313 3181 1047 52 BstBI TT'CG_AA Cuts at: 2007 7397 2007 Size: 5390 3203 BstEII G'GTnAC_C Cuts at: 1184 2974 4418 5419 1184 Size: 1790 1444 1001 4358 Fragments arranged by size: 4358 1790 1444 1001 BstXI CCAn_nnnn'nTGG Cuts at: 4568 4687 4928 5113 4568 Size: 119 241 185 8048 Fragments arranged by size: 8048 241 185 119 BstYI r'GATC_y Cuts at: 1972 2775 4515 5481 5923 6290 6675 6692 8144 Size: 803 1740 966 442 367 385 17 1452 Cuts at: 8144 8230 8242 8588 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1740 1452 966 803 442 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3915 5426 3915 Size: 1511 7082 BtgI C'CryG_G Cuts at: 513 1065 2022 2694 3213 3392 4413 4425 5296 Size: 552 957 672 519 179 1021 12 871 Cuts at: 5296 513 Size: 3810 Fragments arranged by size: 3810 1021 957 871 672 552 519 179 12 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2398 2647 3782 4567 487 Size: 683 56 1172 249 1135 785 4513 Fragments arranged by size: 4513 1172 1135 785 683 249 56 BtsI GCAGTG_nn' Cuts at: 2111 2237 2519 3529 4346 4442 5695 6109 6903 Size: 126 282 1010 817 96 1253 414 794 Cuts at: 6903 6923 2111 Size: 20 3781 Fragments arranged by size: 3781 1253 1010 817 794 414 282 126 96 20 ClaI AT'CG_AT Cuts at: 1554 4998 1554 Size: 3444 5149 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3766 3801 529 Size: 35 3202 35 5321 Fragments arranged by size: 5321 3202 35 35 DraI TTT'AAA Cuts at: 5897 5897 Size: 8593 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 7600 809 Size: 507 6284 1802 Fragments arranged by size: 6284 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2025 2078 2166 Size: 54 1425 50 275 18 195 53 88 Cuts at: 2166 3210 3351 3708 4299 4844 6040 6929 8558 Size: 1044 141 357 591 545 1196 889 1629 Cuts at: 8558 8 Size: 43 Fragments arranged by size: 1629 1425 1196 1044 889 591 545 357 275 195 141 88 54 53 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2078 6040 1537 Size: 275 266 3962 4090 Fragments arranged by size: 4090 3962 275 266 EarI CTCTTCn'nnn_ Cuts at: 1353 2741 3006 6523 1353 Size: 1388 265 3517 3423 Fragments arranged by size: 3517 3423 1388 265 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2498 3669 7180 7554 7700 387 Size: 1064 138 909 1171 3511 374 146 1280 Fragments arranged by size: 3511 1280 1171 1064 909 374 146 138 EcoICRI GAG'CTC Cuts at: 4609 4705 4609 Size: 96 8497 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2237 2290 2483 2524 2540 2579 4410 Size: 393 605 53 193 41 16 39 1831 Cuts at: 4410 4646 5089 5331 5366 5644 5686 5699 6656 Size: 236 443 242 35 278 42 13 957 Cuts at: 6656 7253 8040 8308 1239 Size: 597 787 268 1524 Fragments arranged by size: 1831 1524 957 787 605 597 443 393 278 268 242 236 193 53 42 41 39 35 16 13 EcoNI CCTnn'n_nnAGG Cuts at: 4523 4523 Size: 8593 EcoO109I rG'GnC_Cy Cuts at: 2804 3403 3545 4144 4145 4351 4591 5305 5645 Size: 599 142 599 1 206 240 714 340 Cuts at: 5645 2804 Size: 5752 Fragments arranged by size: 5752 714 599 599 340 240 206 142 1 EcoRI G'AATT_C Cuts at: 5228 5902 6280 5228 Size: 674 378 7541 Fragments arranged by size: 7541 674 378 EcoRV GAT'ATC Cuts at: 4396 4396 Size: 8593 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3040 4960 3040 Size: 1920 6673 FspI TGC'GCA Cuts at: 3521 7099 3521 Size: 3578 5015 HaeII r_GCGC'y Cuts at: 1678 2040 2175 2280 2702 4816 5028 7740 1678 Size: 362 135 105 422 2114 212 2712 2531 Fragments arranged by size: 2712 2531 2114 422 362 212 135 105 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2968 3000 3223 3255 3791 Size: 32 135 32 1396 32 223 32 536 Cuts at: 3791 3823 4097 4129 4535 4567 4694 4726 5240 Size: 32 274 32 406 32 127 32 514 Cuts at: 5240 5272 5422 5454 5473 5505 7234 7266 7308 Size: 32 150 32 19 32 1729 32 42 Cuts at: 7308 7340 1373 Size: 32 2626 Fragments arranged by size: 2626 1729 1396 536 514 406 274 223 150 135 127 42 32 32 32 32 32 32 32 32 32 32 32 32 32 19 HincII GTy'rAC Cuts at: 669 1657 1938 5937 6183 669 Size: 988 281 3999 246 3079 Fragments arranged by size: 3999 3079 988 281 246 HindIII A'AGCT_T Cuts at: 748 3356 3667 748 Size: 2608 311 5674 Fragments arranged by size: 5674 2608 311 HpaI GTT'AAC Cuts at: 6183 6183 Size: 8593 KasI G'GCGC_C Cuts at: 2036 2171 2276 2036 Size: 135 105 8353 Fragments arranged by size: 8353 135 105 KpnI G_GTAC'C Cuts at: 5918 5918 Size: 8593 MfeI C'AATT_G Cuts at: 6192 6192 Size: 8593 MluI A'CGCG_T Cuts at: 8469 8469 Size: 8593 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3360 4402 5190 7707 7891 1305 Size: 2055 1042 788 2517 184 2007 Fragments arranged by size: 2517 2055 2007 1042 788 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3212 3710 10 Size: 54 1425 343 1380 498 4893 Fragments arranged by size: 4893 1425 1380 498 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2417 2645 2864 3513 4064 4874 5543 Size: 648 1251 228 219 649 551 810 669 Cuts at: 5543 6551 6910 7069 518 Size: 1008 359 159 2042 Fragments arranged by size: 2042 1251 1008 810 669 649 648 551 359 228 219 159 MspA1I CmG'CkG Cuts at: 1606 2122 2161 2183 2480 2569 3100 3471 3714 Size: 516 39 22 297 89 531 371 243 Cuts at: 3714 4009 4297 4324 4654 4861 5285 6009 6686 Size: 295 288 27 330 207 424 724 677 Cuts at: 6686 7834 8079 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1148 724 677 531 516 424 371 330 297 295 288 245 243 207 89 39 27 22 NaeI GCC'GGC Cuts at: 2035 2170 2213 2356 4844 2035 Size: 135 43 143 2488 5784 Fragments arranged by size: 5784 2488 143 135 43 NarI GG'CG_CC Cuts at: 2037 2172 2277 2037 Size: 135 105 8353 Fragments arranged by size: 8353 135 105 NcoI C'CATG_G Cuts at: 513 1065 2022 3213 3392 4413 4425 5296 513 Size: 552 957 1191 179 1021 12 871 3810 Fragments arranged by size: 3810 1191 1021 957 871 552 179 12 NdeI CA'TA_TG Cuts at: 387 387 Size: 8593 NgoMIV G'CCGG_C Cuts at: 2033 2168 2211 2354 4842 2033 Size: 135 43 143 2488 5784 Fragments arranged by size: 5784 2488 143 135 43 NheI G'CTAG_C Cuts at: 1052 1052 Size: 8593 NotI GC'GGCC_GC Cuts at: 6040 6040 Size: 8593 NspI r_CATG'y Cuts at: 1132 1229 3269 4232 5951 7496 1132 Size: 97 2040 963 1719 1545 2229 Fragments arranged by size: 2229 2040 1719 1545 963 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 8593 PciI A'CATG_T Cuts at: 7492 7492 Size: 8593 PflMI CCAn_nnn'nTGG Cuts at: 1108 2986 5150 1108 Size: 1878 2164 4551 Fragments arranged by size: 4551 2164 1878 PfoI T'CCnGG_A Cuts at: 2595 2874 2889 2595 Size: 279 15 8299 Fragments arranged by size: 8299 279 15 PmeI GTTT'AAAC Cuts at: 5897 5897 Size: 8593 PmlI CAC'GTG Cuts at: 5829 5829 Size: 8593 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2812 2844 6662 6694 8175 8207 2812 Size: 32 3818 32 1481 32 3198 Fragments arranged by size: 3818 3198 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2804 3403 3545 4351 5305 2804 Size: 599 142 806 954 6092 Fragments arranged by size: 6092 954 806 599 142 PshAI GACnn'nnGTC Cuts at: 1613 5159 1613 Size: 3546 5047 PsiI TTA'TAA Cuts at: 6163 6163 Size: 8593 PspOMI G'GGCC_C Cuts at: 4144 5645 4144 Size: 1501 7092 PstI C_TGCA'G Cuts at: 830 3751 4020 5057 5070 5181 5788 5945 830 Size: 2921 269 1037 13 111 607 157 3478 Fragments arranged by size: 3478 2921 1037 607 269 157 111 13 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8593 PvuII CAG'CTG Cuts at: 2569 3100 3471 3714 4009 4297 4654 4861 5285 Size: 531 371 243 295 288 357 207 424 Cuts at: 5285 2569 Size: 5877 Fragments arranged by size: 5877 531 424 371 357 295 288 243 207 SacI G_AGCT'C Cuts at: 4611 4707 4611 Size: 96 8497 SalI G'TCGA_C Cuts at: 1936 5935 1936 Size: 3999 4594 SanDI GG'GwC_CC Cuts at: 2804 2804 Size: 8593 SapI GCTCTTCn'nnn_ Cuts at: 2741 2741 Size: 8593 SbfI CC_TGCA'GG Cuts at: 5788 5945 5788 Size: 157 8436 ScaI AGT'ACT Cuts at: 5110 6841 8553 5110 Size: 1731 1712 5150 Fragments arranged by size: 5150 1731 1712 SfcI C'TryA_G Cuts at: 826 1045 3747 4016 5053 5066 5177 5784 5941 Size: 219 2702 269 1037 13 111 607 157 Cuts at: 5941 7076 7757 7948 826 Size: 1135 681 191 1471 Fragments arranged by size: 2702 1471 1135 1037 681 607 269 219 191 157 111 13 SfoI GGC'GCC Cuts at: 2038 2173 2278 2038 Size: 135 105 8353 Fragments arranged by size: 8353 135 105 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8593 SgrAI Cr'CCGG_yG Cuts at: 1700 2033 1700 Size: 333 8260 SmaI CCC'GGG Cuts at: 1872 2100 2337 5920 1872 Size: 228 237 3583 4545 Fragments arranged by size: 4545 3583 237 228 SmlI C'TyrA_G Cuts at: 820 1017 1943 3566 4511 4706 5810 6697 7598 Size: 197 926 1623 945 195 1104 887 901 Cuts at: 7598 7860 820 Size: 262 1553 Fragments arranged by size: 1623 1553 1104 945 926 901 887 262 197 195 SnaBI TAC'GTA Cuts at: 493 5890 6384 493 Size: 5397 494 2702 Fragments arranged by size: 5397 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 8593 SphI G_CATG'C Cuts at: 1132 5951 1132 Size: 4819 3774 SspI AAT'ATT Cuts at: 5 52 6517 5 Size: 47 6465 2081 Fragments arranged by size: 6465 2081 47 StuI AGG'CCT Cuts at: 1517 3645 4943 1517 Size: 2128 1298 5167 Fragments arranged by size: 5167 2128 1298 StyI C'CwwG_G Cuts at: 513 1065 2022 2927 3060 3213 3392 3548 3640 Size: 552 957 905 133 153 179 156 92 Cuts at: 3640 4237 4413 4425 4524 5121 5157 5296 5877 Size: 597 176 12 99 597 36 139 581 Cuts at: 5877 6032 513 Size: 155 3074 Fragments arranged by size: 3074 957 905 597 597 581 552 179 176 156 155 153 139 133 99 92 36 12 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2642 3385 3737 5119 6620 6637 6790 Size: 1255 1062 743 352 1382 1501 17 153 Cuts at: 6790 6975 7420 325 Size: 185 445 1498 Fragments arranged by size: 1501 1498 1382 1255 1062 743 445 352 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2535 4089 5108 Size: 274 80 33 51 1443 558 1554 1019 Cuts at: 5108 5217 5493 6839 8551 96 Size: 109 276 1346 1712 138 Fragments arranged by size: 1712 1554 1443 1346 1019 558 276 274 138 109 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4325 4357 4556 4588 8553 8585 4325 Size: 32 199 32 3965 32 4333 Fragments arranged by size: 4333 3965 199 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 5618 1316 Size: 4302 4291 XbaI T'CTAG_A Cuts at: 5929 5929 Size: 8593 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3703 4301 4385 3703 Size: 598 84 7911 Fragments arranged by size: 7911 598 84 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 8593 XmaI C'CCGG_G Cuts at: 1870 2098 2335 5918 1870 Size: 228 237 3583 4545 Fragments arranged by size: 4545 3583 237 228 XmnI GAAnn'nnTTC Cuts at: 3612 4274 6722 3612 Size: 662 2448 5483 Fragments arranged by size: 5483 2448 662 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3069 6401 276 Size: 53 83 186 936 1535 3332 2468 Fragments arranged by size: 3332 2468 1535 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI BfrBI BplI BstZ17I DraIII FseI FspAI NruI NsiI PacI PspXI PsrI RsrII SacII SexAI SfiI SrfI SwaI