Restriction map for
pFN21AE2502
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# NO CUT ENZYMES ( 87 enzymes) #
##################################
AatII Acc65I AclI AflII AflIII
AgeI AhdI Aor51HI AscI Asp718I
AspEI AspI AvrII BbsI BcgI
BclI BfrI BlnI BlpI Bpu1102I
BpuAI BsaBI BsiWI BsmBI BsmI
BspDI BspHI BspLU11I BsrDI BssHII
BssSI Bst1107I BstAPI BstBI BstZ17I
CelII ClaI CpoI DraI DrdI
DsaI Eam1105I Eco47III EcoRV EcoT22I
FbaI FseI HindIII HpaI KpnI
KspI MamI MfeI MluI MunI
NcoI NdeI NotI NruI NsiI
NspV PacI PflFI PinAI PmeI
PshAI Psp1406I PstI PvuI RcaI
RsrII SacII SapI SbfI ScaI
SfcI SfiI SfuI SgrAI SnaBI
SpeI Sse8387I SspI SwaI TfiI
Tth111I XbaI
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# ONE CUT ENZYMES ( 60 enzymes) #
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AccII 2013 CGCG CG^CG
AccIII 1994 TCCGGA T^CCGGA
AcsI 751 GAATTC [GA]^AATT[CT]
Alw44I 1842 GTGCAC G^TGCAC
AlwNI 1312 CAGGCACTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 1034 GGGCCC GGGCC^C
ApaLI 1842 GTGCAC G^TGCAC
ApoI 751 GAATTC [GA]^AATT[CT]
AseI 1704 ATTAAT AT^TAAT
AsnI 1704 ATTAAT AT^TAAT
Asp700I 1260 GAAGCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AviII 275 TGCGCA TGC^GCA
BamHI 1991 GGATCC G^GATCC
BbeI 1691 GGCGCC GGCGC^C
BbrPI 897 CACGTG CAC^GTG
BbvCI 981 CCTCAGC CCTCAGC(-5/-2)
BciVI 22 GGATAC GTATCC(6/5)
BglI 1556 GCCTGTGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI 897 CACGTG [CT]AC^GT[GA]
BsaWI 1994 TCCGGA [AT]^CCGG[AT]
BseAI 1994 TCCGGA T^CCGGA
BsgI 1636 CTGCAC GTGCAG(16/14)
BsiEI 937 CGGCCG CG[GA][CT]^CG
BspEI 1994 TCCGGA T^CCGGA
BsrBI 1352 GAGCGG CCGCTC(-3/-3)
BstEII 1218 GGTGACC G^GT[ACGT]ACC
BstPI 1218 GGTGACC G^GT[ACGT]ACC
BstUI 2013 CGCG CG^CG
DraIII 472 CACAGTGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 937 CGGCCG C^GGCCG
EarI 1984 GAAGAG CTCTTC(1/4)
EclXI 937 CGGCCG C^GGCCG
Eco52I 937 CGGCCG C^GGCCG
EcoNI 459 CCTGAAGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 1218 GGTGACC G^GT[ACGT]ACC
EcoRI 751 GAATTC G^AATTC
FspI 275 TGCGCA TGC^GCA
KasI 1691 GGCGCC G^GCGCC
Ksp632I 1984 GAAGAG CTCTTC(1/4)
MaeII 898 ACGT A^CGT
MroI 1994 TCCGGA T^CCGGA
MseI 1705 TTAA T^TAA
MvnI 2013 CGCG CG^CG
NarI 1691 GGCGCC GG^CGCC
NheI 1296 GCTAGC G^CTAGC
NspI 50 GCATGC [GA]CATG^[CT]
PmaCI 897 CACGTG CAC^GTG
PmlI 897 CACGTG CAC^GTG
PshBI 1704 ATTAAT AT^TAAT
SacI 184 GAGCTC GAGCT^C
SalI 628 GTCGAC G^TCGAC
SexAI 1289 ACCTGGT A^CC[AT]GGT
SfoI 1691 GGCGCC GGC^GCC
SmaI 1127 CCCGGG CCC^GGG
SphI 50 GCATGC GCATG^C
StuI 581 AGGCCT AGG^CCT
Tru9I 1705 TTAA T^TAA
XmaCI 1127 CCCGGG C^CCGGG
XmaI 1127 CCCGGG C^CCGGG
XmnI 1260 GAAGCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AccI 628 GTCGAC GT^[AC][GT]AC
838 GTCTAC
AocI 778 CCTGAGG CC^T[ACGT]AGG
1436 CCTCAGG
AspHI 184 GAGCTC G[AT]GC[AT]^C
1842 GTGCAC
BalI 479 TGGCCA TGG^CCA
728 TGGCCA
BfaI 835 CTAG C^TAG
1297 CTAG
BpmI 227 CTCCAG CTGGAG(16/14)
14 CTGGAG
BsiHKAI 184 GAGCTC G[AT]GC[AT]^C
1842 GTGCAC
Bsp1407I 737 TGTACA T^GTACA
1478 TGTACA
BsrGI 737 TGTACA T^GTACA
1478 TGTACA
BstXI 47 CCAGCATGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1571 CCATATTCATGG
Bsu36I 778 CCTGAGG CC^T[ACGT]AGG
1436 CCTCAGG
Eco81I 778 CCTGAGG CC^T[ACGT]AGG
1436 CCTCAGG
HincII 628 GTCGAC GT[CT]^[GA]AC
2002 GTCAAC
HindII 628 GTCGAC GT[CT]^[GA]AC
2002 GTCAAC
MaeI 835 CTAG C^TAG
1297 CTAG
MluNI 479 TGGCCA TGG^CCA
728 TGGCCA
MscI 479 TGGCCA TGG^CCA
728 TGGCCA
NaeI 83 GCCGGC GCC^GGC
939 GCCGGC
NgoMIV 83 GCCGGC G^CCGGC
939 GCCGGC
PaeR7I 181 CTCGAG C^TCGAG
2053 CTCGAG
SmlI 181 CTCGAG C^T[CT][GA]AG
2053 CTCGAG
SspBI 737 TGTACA T^GTACA
1478 TGTACA
XcmI 614 CCAATGTCATCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1106 CCACATCACACATGG
XhoI 181 CTCGAG C^TCGAG
2053 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 87 enzymes) #
#########################################
AciI AcyI AfaI AluI AlwI
AvaI AvaII BanI BanII BbvI
BcnI BglII BmyI BsaHI BsaI
BsaJI BseRI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspMI BsrFI
BsrI BssKI BstF5I BstNI BstYI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII EaeI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
MaeIII MboI MboII MflI MnlI
MslI MspA1I MspI MvaI MwoI
NciI NdeII NlaIII NlaIV PflMI
PleI PpuMI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI StyI TaqI
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XhoII