Restriction map for
pFN21AE2502
(Circular) (Six-base) MAPSORT of: pFN21AE2502.seq Check: 6403 from: 1 to: 6877
pFN21AE2502 6877 bp
With 182 enzymes: SgfI *
February 16, 2012 14:31 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2534 3254 2534
Size: 720 6157
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4687 278
Size: 53 83 186 936 3151 2468
Fragments arranged by size:
3151 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2634 2844 4220 1937
Size: 697 210 1376 4594
Fragments arranged by size:
4594 1376 697 210
Acc65I G'GTAC_C
Cuts at: 4198 4198
Size: 6877
AclI AA'CG_TT
Cuts at: 1563 5004 5377 1563
Size: 3441 373 3063
Fragments arranged by size:
3441 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2474 2486 2684 3092 3284 4940 6324 6592
Size: 842 12 198 408 192 1656 1384 268
Cuts at: 6592 1632
Size: 1917
Fragments arranged by size:
1917 1656 1384 842 408 268 198 192
12
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6680
AflIII A'CryG_T
Cuts at: 1203 5776 6753 1203
Size: 4573 977 1327
Fragments arranged by size:
4573 1327 977
AgeI A'CCGG_T
Cuts at: 5708 5708
Size: 6877
AhdI GACnn_n'nnGTC
Cuts at: 5606 5606
Size: 6877
AleI CACnn'nnGTG
Cuts at: 1166 3648 3938 1166
Size: 2482 290 4105
Fragments arranged by size:
4105 2482 290
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3518 3812 1184
Size: 2334 294 4249
Fragments arranged by size:
4249 2334 294
AlwNI CAG_nnn'CTG
Cuts at: 3322 4620 6192 3322
Size: 1298 1572 4007
Fragments arranged by size:
4007 1572 1298
ApaI G_GGCC'C
Cuts at: 3043 3043
Size: 6877
ApaLI G'TGCA_C
Cuts at: 3847 4934 6090 3847
Size: 1087 1156 4634
Fragments arranged by size:
4634 1156 1087
ApoI r'AATT_y
Cuts at: 1493 2756 4186 4412 4564 6673 1493
Size: 1263 1430 226 152 2109 1697
Fragments arranged by size:
2109 1697 1430 1263 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3710 5431 160
Size: 872 2678 1721 1606
Fragments arranged by size:
2678 1721 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6877
AvaI C'yCGr_G
Cuts at: 1870 1943 2186 2256 3132 4058 4202 1870
Size: 73 243 70 876 926 144 4545
Fragments arranged by size:
4545 926 876 243 144 73 70
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 4126 4159 4126
Size: 33 6844
BamHI G'GATC_C
Cuts at: 3996 4207 4574 3996
Size: 211 367 6299
Fragments arranged by size:
6299 367 211
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2855 3127 3502 3696 4198
Size: 325 217 627 1068 272 375 194 502
Cuts at: 4198 5653 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 1068 627 502 375 325 272
217 194
BanII G_rGCy'C
Cuts at: 1391 2193 3043 3111 4196 1391
Size: 802 850 68 1085 4072
Fragments arranged by size:
4072 1085 850 802 68
BbeI G_GCGC'C
Cuts at: 3700 3700
Size: 6877
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5906
BbvCI CC'TCA_GC
Cuts at: 2987 2987
Size: 6877
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 5068 5102 1903
Size: 34 50 34 3047 34 3678
Fragments arranged by size:
3678 3047 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2021 4769 5979 2021
Size: 2748 1210 2919
Fragments arranged by size:
2919 2748 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6877
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3567 5488 136
Size: 107 122 71 631 605 1895 1921 1525
Fragments arranged by size:
1921 1895 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2667 3006 3555 6872 2667
Size: 339 549 3317 2672
Fragments arranged by size:
3317 2672 549 339
BlpI GC'TnA_GC
Cuts at: 4294 4294
Size: 6877
Bme1580I G_kGCm'C
Cuts at: 1790 2534 3043 3132 3507 3741 3750 3851 4938
Size: 744 509 89 375 234 9 101 1087
Cuts at: 4938 6094 1790
Size: 1156 2573
Fragments arranged by size:
2573 1156 1087 744 509 375 234 101
89 9
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6877
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2215 2871 3332
Size: 416 53 83 399 24 798 656 461
Cuts at: 3332 3702 4608 5566 442
Size: 370 906 958 1753
Fragments arranged by size:
1753 958 906 798 656 461 416 399
370 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 3305 1056
Size: 2249 4628
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2040 2217 5537 1239
Size: 801 177 3320 2579
Fragments arranged by size:
3320 2579 801 177
Bpu10I CC'TnA_GC
Cuts at: 2987 3490 2987
Size: 503 6374
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5002 5867 6165 5002
Size: 865 298 5714
Fragments arranged by size:
5714 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2066 3404 3431 5540 882
Size: 617 567 1338 27 2109 2219
Fragments arranged by size:
2219 2109 1338 617 567 27
BsaAI yAC'GTr
Cuts at: 493 1204 2904 4668 493
Size: 711 1700 1764 2702
Fragments arranged by size:
2702 1764 1700 711
BsaBI GATnn'nnATC
Cuts at: 6871 6871
Size: 6877
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2799 3066 3669 3697
Size: 53 83 186 936 1266 267 603 28
Cuts at: 3697 4684 5066 5701 275
Size: 987 382 635 1451
Fragments arranged by size:
1451 1266 987 936 635 603 382 267
186 83 53 28
BsaWI w'CCGG_w
Cuts at: 3999 5310 5708 5982 6129 6540 3999
Size: 1311 398 274 147 411 4336
Fragments arranged by size:
4336 1311 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2231 2261 1178
Size: 30 165 30 828 30 5794
Fragments arranged by size:
5794 828 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2252 2709 3770 1186
Size: 1066 457 1061 4293
Fragments arranged by size:
4293 1066 1061 457
BseYI C'CCAG_C
Cuts at: 2051 2291 2767 2814 6080 6576 2051
Size: 240 476 47 3266 496 2352
Fragments arranged by size:
3266 2352 496 476 240 47
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3626 1163
Size: 573 1890 4414
Fragments arranged by size:
4414 1890 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2945 4327 5088 6116 1540
Size: 275 186 944 1382 761 1028 2301
Fragments arranged by size:
2301 1382 1028 944 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2193 3851 4938 5023 6094 2193
Size: 1658 1087 85 1071 2976
Fragments arranged by size:
2976 1658 1087 1071 85
BsmI GAATG_Cn'
Cuts at: 4388 4481 4388
Size: 93 6784
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2193 2534 3043 3111 3132 3507 3741
Size: 399 403 341 509 68 21 375 234
Cuts at: 3741 3750 3851 4196 4938 5023 6094 1391
Size: 9 101 345 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 509 403 399 375 345
341 234 101 85 68 21 9
BspEI T'CCGG_A
Cuts at: 3999 6540 3999
Size: 2541 4336
BspHI T'CATG_A
Cuts at: 4766 6496 4766
Size: 1730 5147
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2430 2534 2988 3254 4232 844
Size: 1586 104 454 266 978 3489
Fragments arranged by size:
3489 1586 978 454 266 104
BsrBI CCG'CTC
Cuts at: 1394 1823 3359 4764 1394
Size: 429 1536 1405 3507
Fragments arranged by size:
3507 1536 1405 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5372 5546 65
Size: 1183 4124 174 1396
Fragments arranged by size:
4124 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2088 2930 2944 3477 5521 5708 1700
Size: 110 278 842 14 533 2044 187 2869
Fragments arranged by size:
2869 2044 842 533 278 187 110 14
BsrGI T'GTAC_A
Cuts at: 96 2742 3483 96
Size: 2646 741 3490
Fragments arranged by size:
3490 2646 741
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6877
BssSI C'ACGA_G
Cuts at: 4937 5949 4937
Size: 1012 5865
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6877
BstBI TT'CG_AA
Cuts at: 5681 5681
Size: 6877
BstEII G'GTnAC_C
Cuts at: 1184 3223 1184
Size: 2039 4838
BstXI CCAn_nnnn'nTGG
Cuts at: 2059 3583 2059
Size: 1524 5353
BstYI r'GATC_y
Cuts at: 1972 2658 2667 3006 3555 3996 4207 4574 4959
Size: 686 9 339 549 441 211 367 385
Cuts at: 4959 4976 6428 6514 6526 6872 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 686 549 441 385 367 346
339 211 86 17 12 9
Bsu36I CC'TnA_GG
Cuts at: 2784 3442 2784
Size: 658 6219
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 6325
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2347 2584 487
Size: 683 56 1121 237 4780
Fragments arranged by size:
4780 1121 683 237 56
BtsI GCAGTG_nn'
Cuts at: 2532 4393 5187 5207 2532
Size: 1861 794 20 4202
Fragments arranged by size:
4202 1861 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6877
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6842
DraI TTT'AAA
Cuts at: 4181 4181
Size: 6877
DraIII CAC_nnn'GTG
Cuts at: 2482 2482
Size: 6877
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5884 809
Size: 507 4568 1802
Fragments arranged by size:
4568 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2484 2733 2942
Size: 54 1425 50 275 18 654 249 209
Cuts at: 2942 3658 4324 5213 6842 8
Size: 716 666 889 1629 43
Fragments arranged by size:
1629 1425 889 716 666 654 275 249
209 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2942 4324 1537
Size: 275 1130 1382 4090
Fragments arranged by size:
4090 1382 1130 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3984 4807 1353
Size: 2631 823 3423
Fragments arranged by size:
3423 2631 823
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5464 5838 5984 387
Size: 1064 138 3875 374 146 1280
Fragments arranged by size:
3875 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2191 2191
Size: 6877
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2040 2217 2474 2486 2684 3092 3284
Size: 393 408 177 257 12 198 408 192
Cuts at: 3284 4940 5537 6324 6592 1239
Size: 1656 597 787 268 1524
Fragments arranged by size:
1656 1524 787 597 408 408 393 268
257 198 192 177 12
EcoNI CCTnn'n_nnAGG
Cuts at: 2468 2468
Size: 6877
EcoO109I rG'GnC_Cy
Cuts at: 2047 2338 2356 3039 3446 4094 2047
Size: 291 18 683 407 648 4830
Fragments arranged by size:
4830 683 648 407 291 18
EcoRI G'AATT_C
Cuts at: 2756 4186 4564 2756
Size: 1430 378 5069
Fragments arranged by size:
5069 1430 378
FspI TGC'GCA
Cuts at: 2282 5383 2282
Size: 3101 3776
HaeII r_GCGC'y
Cuts at: 1678 2246 2383 3700 6024 1678
Size: 568 137 1317 2324 2531
Fragments arranged by size:
2531 2324 1317 568 137
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2872 2904 3098 3130 3868
Size: 32 135 32 1300 32 194 32 738
Cuts at: 3868 3900 5518 5550 5592 5624 1373
Size: 32 1618 32 42 32 2626
Fragments arranged by size:
2626 1618 1300 738 194 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2635 4009 4221 4467 669
Size: 988 281 697 1374 212 246 3079
Fragments arranged by size:
3079 1374 988 697 281 246 212
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6877
HpaI GTT'AAC
Cuts at: 4467 4467
Size: 6877
KasI G'GCGC_C
Cuts at: 3696 3696
Size: 6877
KpnI G_GTAC'C
Cuts at: 4202 4202
Size: 6877
MfeI C'AATT_G
Cuts at: 4476 4476
Size: 6877
MluI A'CGCG_T
Cuts at: 6753 6753
Size: 6877
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5991 6175 1305
Size: 4686 184 2007
Fragments arranged by size:
4686 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2486 2735 10
Size: 54 1425 343 654 249 4152
Fragments arranged by size:
4152 1425 654 343 249 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2102 3125 3221 3581 3648 3938 4835
Size: 648 936 1023 96 360 67 290 897
Cuts at: 4835 5194 5353 518
Size: 359 159 2042
Fragments arranged by size:
2042 1023 936 897 648 360 359 290
159 96 67
MspA1I CmG'CkG
Cuts at: 1606 2295 2650 3722 4293 4970 6118 6363 1606
Size: 689 355 1072 571 677 1148 245 2120
Fragments arranged by size:
2120 1148 1072 689 677 571 355 245
NaeI GCC'GGC
Cuts at: 2090 2946 2090
Size: 856 6021
NarI GG'CG_CC
Cuts at: 3697 3697
Size: 6877
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 6325
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6877
NgoMIV G'CCGG_C
Cuts at: 2088 2944 2088
Size: 856 6021
NheI G'CTAG_C
Cuts at: 1052 3301 1052
Size: 2249 4628
NotI GC'GGCC_GC
Cuts at: 4324 4324
Size: 6877
NspI r_CATG'y
Cuts at: 1132 1229 2059 4235 5780 1132
Size: 97 830 2176 1545 2229
Fragments arranged by size:
2229 2176 1545 830 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6877
PciI A'CATG_T
Cuts at: 5776 5776
Size: 6877
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2482 3169 3322 3919 1108
Size: 1374 687 153 597 4066
Fragments arranged by size:
4066 1374 687 597 153
PfoI T'CCnGG_A
Cuts at: 3162 3162
Size: 6877
PmeI GTTT'AAAC
Cuts at: 4181 4181
Size: 6877
PmlI CAC'GTG
Cuts at: 2904 2904
Size: 6877
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4946 4978 6459 6491 4946
Size: 32 1481 32 5332
Fragments arranged by size:
5332 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2047 2356 4094 2047
Size: 309 1738 4830
Fragments arranged by size:
4830 1738 309
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6877
PsiI TTA'TAA
Cuts at: 4447 4447
Size: 6877
PspOMI G'GGCC_C
Cuts at: 3039 3039
Size: 6877
PstI C_TGCA'G
Cuts at: 830 4229 830
Size: 3399 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6877
PvuII CAG'CTG
Cuts at: 2295 2650 3722 2295
Size: 355 1072 5450
Fragments arranged by size:
5450 1072 355
SacI G_AGCT'C
Cuts at: 2193 2193
Size: 6877
SalI G'TCGA_C
Cuts at: 1936 2633 4219 1936
Size: 697 1586 4594
Fragments arranged by size:
4594 1586 697
SanDI GG'GwC_CC
Cuts at: 2047 4094 2047
Size: 2047 4830
SbfI CC_TGCA'GG
Cuts at: 4229 4229
Size: 6877
ScaI AGT'ACT
Cuts at: 5125 6837 5125
Size: 1712 5165
SexAI A'CCwGG_T
Cuts at: 3294 3294
Size: 6877
SfcI C'TryA_G
Cuts at: 826 1045 4225 5360 6041 6232 826
Size: 219 3180 1135 681 191 1471
Fragments arranged by size:
3180 1471 1135 681 219 191
SfoI GGC'GCC
Cuts at: 3698 3698
Size: 6877
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6877
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6877
SmaI CCC'GGG
Cuts at: 1872 3134 4204 1872
Size: 1262 1070 4545
Fragments arranged by size:
4545 1262 1070
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2186 4058 4981 5882 6144 820
Size: 197 926 243 1872 923 901 262 1553
Fragments arranged by size:
1872 1553 926 923 901 262 243 197
SnaBI TAC'GTA
Cuts at: 493 4668 493
Size: 4175 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6877
SphI G_CATG'C
Cuts at: 1132 2059 4235 1132
Size: 927 2176 3774
Fragments arranged by size:
3774 2176 927
SspI AAT'ATT
Cuts at: 5 52 4801 5
Size: 47 4749 2081
Fragments arranged by size:
4749 2081 47
StuI AGG'CCT
Cuts at: 1517 2588 1517
Size: 1071 5806
StyI C'CwwG_G
Cuts at: 513 1065 2442 2871 3228 3306 4316 513
Size: 552 1377 429 357 78 1010 3074
Fragments arranged by size:
3074 1377 1010 552 429 357 78
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2129 2828 4904 4921 5074 5259 5704
Size: 1255 549 699 2076 17 153 185 445
Cuts at: 5704 325
Size: 1498
Fragments arranged by size:
2076 1498 1255 699 549 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2595 2742 3483
Size: 274 80 33 51 1443 618 147 741
Cuts at: 3483 4134 5123 6835 96
Size: 651 989 1712 138
Fragments arranged by size:
1712 1443 989 741 651 618 274 147
138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2231 2263 3443 3475 6837 6869 2231
Size: 32 1180 32 3362 32 2239
Fragments arranged by size:
3362 2239 1180 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6877
XbaI T'CTAG_A
Cuts at: 4213 4213
Size: 6877
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2626 3118 2626
Size: 492 6385
XhoI C'TCGA_G
Cuts at: 1943 2186 4058 1943
Size: 243 1872 4762
Fragments arranged by size:
4762 1872 243
XmaI C'CCGG_G
Cuts at: 1870 3132 4202 1870
Size: 1262 1070 4545
Fragments arranged by size:
4545 1262 1070
XmnI GAAnn'nnTTC
Cuts at: 3269 5006 3269
Size: 1737 5140
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4685 276
Size: 53 83 186 936 3151 2468
Fragments arranged by size:
3151 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI
NotI NspI PasI PciI PflMI PfoI PmeI PmlI
PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII
SacI SalI SanDI SbfI ScaI SexAI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BfrBI BplI BsiWI BsmBI
BstZ17I EcoRV FalI FseI FspAI NruI NsiI PacI
PspXI PsrI RsrII SacII SapI SfiI SrfI SwaI