Restriction map for
pFN21AE2502
(Linear) (Six-base) MAPSORT of: KIEE2502.seq Check: 2825 from: 1 to: 2172
KIEE2502.orf
With 182 enzymes: SgfI *
February 16, 2012 13:35 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 529 1249 2172
Size: 529 720 923
Fragments arranged by size:
923 720 529
AccI GT'mk_AC
Cuts at: 0 629 839 2172
Size: 629 210 1333
Fragments arranged by size:
1333 629 210
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 469 481 679 1087 1279 2172
Size: 469 12 198 408 192 893
Fragments arranged by size:
893 469 408 198 192 12
AleI CACnn'nnGTG
Cuts at: 0 1643 1933 2172
Size: 1643 290 239
Fragments arranged by size:
1643 290 239
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1513 1807 2172
Size: 1513 294 365
Fragments arranged by size:
1513 365 294
AlwNI CAG_nnn'CTG
Cuts at: 0 1317 2172
Size: 1317 855
ApaI G_GGCC'C
Cuts at: 0 1038 2172
Size: 1038 1134
ApaLI G'TGCA_C
Cuts at: 0 1842 2172
Size: 1842 330
ApoI r'AATT_y
Cuts at: 0 751 2172
Size: 751 1421
AseI AT'TA_AT
Cuts at: 0 1705 2172
Size: 1705 467
AvaI C'yCGr_G
Cuts at: 0 181 251 1127 2053 2172
Size: 181 70 876 926 119
Fragments arranged by size:
926 876 181 119 70
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 2121 2154 2172
Size: 2121 33 18
Fragments arranged by size:
2121 33 18
BamHI G'GATC_C
Cuts at: 0 1991 2172
Size: 1991 181
BanI G'GyrC_C
Cuts at: 0 850 1122 1497 1691 2172
Size: 850 272 375 194 481
Fragments arranged by size:
850 481 375 272 194
BanII G_rGCy'C
Cuts at: 0 188 1038 1106 2172
Size: 188 850 68 1066
Fragments arranged by size:
1066 850 188 68
BbeI G_GCGC'C
Cuts at: 0 1695 2172
Size: 1695 477
BbvCI CC'TCA_GC
Cuts at: 0 982 2172
Size: 982 1190
BciVI GTATCCnnnnn_n'
Cuts at: 0 16 2172
Size: 16 2156
BglI GCCn_nnn'nGGC
Cuts at: 0 1562 2172
Size: 1562 610
BglII A'GATC_T
Cuts at: 0 662 1001 1550 2172
Size: 662 339 549 622
Fragments arranged by size:
662 622 549 339
Bme1580I G_kGCm'C
Cuts at: 0 529 1038 1127 1502 1736 1745 1846 2172
Size: 529 509 89 375 234 9 101 326
Fragments arranged by size:
529 509 375 326 234 101 89 9
BmrI ACTGGGnnnn_n'
Cuts at: 0 210 866 1327 1697 2172
Size: 210 656 461 370 475
Fragments arranged by size:
656 475 461 370 210
BmtI G_CTAG'C
Cuts at: 0 1300 2172
Size: 1300 872
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 35 212 2172
Size: 35 177 1960
Fragments arranged by size:
1960 177 35
Bpu10I CC'TnA_GC
Cuts at: 0 982 1485 2172
Size: 982 503 687
Fragments arranged by size:
982 687 503
BsaI GGTCTCn'nnnn_
Cuts at: 0 61 1399 1426 2172
Size: 61 1338 27 746
Fragments arranged by size:
1338 746 61 27
BsaAI yAC'GTr
Cuts at: 0 899 2172
Size: 899 1273
BsaHI Gr'CG_yC
Cuts at: 0 794 1061 1664 1692 2172
Size: 794 267 603 28 480
Fragments arranged by size:
794 603 480 267 28
BsaWI w'CCGG_w
Cuts at: 0 1994 2172
Size: 1994 178
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 226 256 2172
Size: 226 30 1916
Fragments arranged by size:
1916 226 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 247 704 1765 2172
Size: 247 457 1061 407
Fragments arranged by size:
1061 457 407 247
BseYI C'CCAG_C
Cuts at: 0 46 286 762 809 2172
Size: 46 240 476 47 1363
Fragments arranged by size:
1363 476 240 47 46
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1621 2172
Size: 1621 551
BsiEI CG_ry'CG
Cuts at: 0 940 2172
Size: 940 1232
BsiHKAI G_wGCw'C
Cuts at: 0 188 1846 2172
Size: 188 1658 326
Fragments arranged by size:
1658 326 188
Bsp1286I G_dGCh'C
Cuts at: 0 188 529 1038 1106 1127 1502 1736 1745
Size: 188 341 509 68 21 375 234 9
Cuts at: 1745 1846 2172
Size: 101 326
Fragments arranged by size:
509 375 341 326 234 188 101 68
21 9
BspEI T'CCGG_A
Cuts at: 0 1994 2172
Size: 1994 178
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 425 529 983 1249 2172
Size: 425 104 454 266 923
Fragments arranged by size:
923 454 425 266 104
BsrBI CCG'CTC
Cuts at: 0 1354 2172
Size: 1354 818
BsrFI r'CCGG_y
Cuts at: 0 83 925 939 1472 2172
Size: 83 842 14 533 700
Fragments arranged by size:
842 700 533 83 14
BsrGI T'GTAC_A
Cuts at: 0 737 1478 2172
Size: 737 741 694
Fragments arranged by size:
741 737 694
BstEII G'GTnAC_C
Cuts at: 0 1218 2172
Size: 1218 954
BstXI CCAn_nnnn'nTGG
Cuts at: 0 54 1578 2172
Size: 54 1524 594
Fragments arranged by size:
1524 594 54
BstYI r'GATC_y
Cuts at: 0 653 662 1001 1550 1991 2172
Size: 653 9 339 549 441 181
Fragments arranged by size:
653 549 441 339 181 9
Bsu36I CC'TnA_GG
Cuts at: 0 779 1437 2172
Size: 779 658 735
Fragments arranged by size:
779 735 658
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 342 579 2172
Size: 342 237 1593
Fragments arranged by size:
1593 342 237
BtsI GCAGTG_nn'
Cuts at: 0 527 2172
Size: 527 1645
DraIII CAC_nnn'GTG
Cuts at: 0 477 2172
Size: 477 1695
EaeI y'GGCC_r
Cuts at: 0 479 728 937 1653 2172
Size: 479 249 209 716 519
Fragments arranged by size:
716 519 479 249 209
EagI C'GGCC_G
Cuts at: 0 937 2172
Size: 937 1235
EarI CTCTTCn'nnn_
Cuts at: 0 1979 2172
Size: 1979 193
EcoICRI GAG'CTC
Cuts at: 0 186 2172
Size: 186 1986
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 35 212 469 481 679 1087 1279 2172
Size: 35 177 257 12 198 408 192 893
Fragments arranged by size:
893 408 257 198 192 177 35 12
EcoNI CCTnn'n_nnAGG
Cuts at: 0 463 2172
Size: 463 1709
EcoO109I rG'GnC_Cy
Cuts at: 0 42 333 351 1034 1441 2089 2172
Size: 42 291 18 683 407 648 83
Fragments arranged by size:
683 648 407 291 83 42 18
EcoRI G'AATT_C
Cuts at: 0 751 2172
Size: 751 1421
FspI TGC'GCA
Cuts at: 0 277 2172
Size: 277 1895
HaeII r_GCGC'y
Cuts at: 0 241 378 1695 2172
Size: 241 137 1317 477
Fragments arranged by size:
1317 477 241 137
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 867 899 1093 1125 1863 1895 2172
Size: 867 32 194 32 738 32 277
Fragments arranged by size:
867 738 277 194 32 32 32
HincII GTy'rAC
Cuts at: 0 630 2004 2172
Size: 630 1374 168
Fragments arranged by size:
1374 630 168
KasI G'GCGC_C
Cuts at: 0 1691 2172
Size: 1691 481
MscI TGG'CCA
Cuts at: 0 481 730 2172
Size: 481 249 1442
Fragments arranged by size:
1442 481 249
MslI CAynn'nnrTG
Cuts at: 0 97 1120 1216 1576 1643 1933 2172
Size: 97 1023 96 360 67 290 239
Fragments arranged by size:
1023 360 290 239 97 96 67
MspA1I CmG'CkG
Cuts at: 0 290 645 1717 2172
Size: 290 355 1072 455
Fragments arranged by size:
1072 455 355 290
NaeI GCC'GGC
Cuts at: 0 85 941 2172
Size: 85 856 1231
Fragments arranged by size:
1231 856 85
NarI GG'CG_CC
Cuts at: 0 1692 2172
Size: 1692 480
NgoMIV G'CCGG_C
Cuts at: 0 83 939 2172
Size: 83 856 1233
Fragments arranged by size:
1233 856 83
NheI G'CTAG_C
Cuts at: 0 1296 2172
Size: 1296 876
NspI r_CATG'y
Cuts at: 0 54 2172
Size: 54 2118
PflMI CCAn_nnn'nTGG
Cuts at: 0 477 1164 1317 1914 2172
Size: 477 687 153 597 258
Fragments arranged by size:
687 597 477 258 153
PfoI T'CCnGG_A
Cuts at: 0 1157 2172
Size: 1157 1015
PmlI CAC'GTG
Cuts at: 0 899 2172
Size: 899 1273
PpuMI rG'GwC_Cy
Cuts at: 0 42 351 2089 2172
Size: 42 309 1738 83
Fragments arranged by size:
1738 309 83 42
PspOMI G'GGCC_C
Cuts at: 0 1034 2172
Size: 1034 1138
PvuII CAG'CTG
Cuts at: 0 290 645 1717 2172
Size: 290 355 1072 455
Fragments arranged by size:
1072 455 355 290
SacI G_AGCT'C
Cuts at: 0 188 2172
Size: 188 1984
SalI G'TCGA_C
Cuts at: 0 628 2172
Size: 628 1544
SanDI GG'GwC_CC
Cuts at: 0 42 2089 2172
Size: 42 2047 83
Fragments arranged by size:
2047 83 42
SexAI A'CCwGG_T
Cuts at: 0 1289 2172
Size: 1289 883
SfoI GGC'GCC
Cuts at: 0 1693 2172
Size: 1693 479
SmaI CCC'GGG
Cuts at: 0 1129 2172
Size: 1129 1043
SmlI C'TyrA_G
Cuts at: 0 181 2053 2172
Size: 181 1872 119
Fragments arranged by size:
1872 181 119
SphI G_CATG'C
Cuts at: 0 54 2172
Size: 54 2118
StuI AGG'CCT
Cuts at: 0 583 2172
Size: 583 1589
StyI C'CwwG_G
Cuts at: 0 437 866 1223 1301 2172
Size: 437 429 357 78 871
Fragments arranged by size:
871 437 429 357 78
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 124 823 2172
Size: 124 699 1349
Fragments arranged by size:
1349 699 124
TatI w'GTAC_w
Cuts at: 0 590 737 1478 2129 2172
Size: 590 147 741 651 43
Fragments arranged by size:
741 651 590 147 43
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 226 258 1438 1470 2172
Size: 226 32 1180 32 702
Fragments arranged by size:
1180 702 226 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 621 1113 2172
Size: 621 492 1059
Fragments arranged by size:
1059 621 492
XhoI C'TCGA_G
Cuts at: 0 181 2053 2172
Size: 181 1872 119
Fragments arranged by size:
1872 181 119
XmaI C'CCGG_G
Cuts at: 0 1127 2172
Size: 1127 1045
XmnI GAAnn'nnTTC
Cuts at: 0 1264 2172
Size: 1264 908
Enzymes that do cut:
AarI AccI AcuI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AvaI BaeI BamHI BanI BanII BbeI
BbvCI BciVI BglI BglII Bme1580I BmrI BmtI BpmI
Bpu10I BsaI BsaAI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI Bsp1286I BspEI BspMI BsrBI BsrFI
BsrGI BstEII BstXI BstYI Bsu36I BtgZI BtsI DraIII
EaeI EagI EarI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FspI HaeII Hin4I HincII KasI MscI MslI MspA1I
NaeI NarI NgoMIV NheI NspI PflMI PfoI PmlI
PpuMI PspOMI PvuII SacI SalI SanDI SexAI SfoI
SmaI SmlI SphI StuI StyI TaqII TatI TstI
XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AflIII AgeI AhdI
AloI AscI AsiSI AvrII BbsI BcgI BclI BfrBI
BlpI BmgBI BplI BpuEI BsaBI BsiWI BsmI BsmBI
BspHI BsrDI BssHII BssSI BstAPI BstBI BstZ17I BtgI
ClaI CspCI DraI DrdI EciI EcoRV FalI FseI
FspAI HindIII HpaI KpnI MfeI MluI MmeI NcoI
NdeI NotI NruI NsiI PacI PasI PciI PmeI
PpiI PshAI PsiI PspXI PsrI PstI PvuI RsrII
SacII SapI SbfI ScaI SfcI SfiI SgfI SgrAI
SnaBI SpeI SrfI SspI SwaI Tth111I XbaI ZraI