Restriction map for
pFN21ASDA0620
(Circular) (Six-base) MAPSORT of: pFN21ASDA0620.seq Check: 7340 from: 1 to: 10504
pFN21ASDA0620 10504 bp
With 182 enzymes: SgfI *
January 16, 2009 15:30 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3378 3523 4691 5399 7738 3378
Size: 145 1168 708 2339 6144
Fragments arranged by size:
6144 2339 1168 708 145
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3635 6840 8314 278
Size: 53 83 186 936 2099 3205 1474 2468
Fragments arranged by size:
3205 2468 2099 1474 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 6840 7847 1937
Size: 4903 1007 4594
Fragments arranged by size:
4903 4594 1007
Acc65I G'GTAC_C
Cuts at: 7825 7825
Size: 10504
AclI AA'CG_TT
Cuts at: 1563 8631 9004 1563
Size: 7068 373 3063
Fragments arranged by size:
7068 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3422 6978 7370 8567 9951 10219 1632
Size: 1790 3556 392 1197 1384 268 1917
Fragments arranged by size:
3556 1917 1790 1384 1197 392 268
AfeI AGC'GCT
Cuts at: 7532 7532
Size: 10504
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 10307
AflIII A'CryG_T
Cuts at: 1203 2586 2910 6699 9403 10380 1203
Size: 1383 324 3789 2704 977 1327
Fragments arranged by size:
3789 2704 1383 1327 977 324
AgeI A'CCGG_T
Cuts at: 2663 9335 2663
Size: 6672 3832
AhdI GACnn_n'nnGTC
Cuts at: 9233 9233
Size: 10504
AleI CACnn'nnGTG
Cuts at: 1166 3413 3440 4218 6902 7634 1166
Size: 2247 27 778 2684 732 4036
Fragments arranged by size:
4036 2684 2247 778 732 27
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2657 3005 3899 4028 5882 6821 7130 1184
Size: 1473 348 894 129 1854 939 309 4558
Fragments arranged by size:
4558 1854 1473 939 894 348 309 129
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2011 2043 7169 7201 2011
Size: 32 5126 32 5314
Fragments arranged by size:
5314 5126 32 32
AlwNI CAG_nnn'CTG
Cuts at: 2428 3400 3819 3977 4600 5065 5291 6485 8247
Size: 972 419 158 623 465 226 1194 1762
Cuts at: 8247 9819 2428
Size: 1572 3113
Fragments arranged by size:
3113 1762 1572 1194 972 623 465 419
226 158
ApaI G_GGCC'C
Cuts at: 2312 3812 4997 5143 5410 2312
Size: 1500 1185 146 267 7406
Fragments arranged by size:
7406 1500 1185 267 146
ApaLI G'TGCA_C
Cuts at: 2898 3578 3674 3745 5192 8561 9717 2898
Size: 680 96 71 1447 3369 1156 3685
Fragments arranged by size:
3685 3369 1447 1156 680 96 71
ApoI r'AATT_y
Cuts at: 1493 7813 8039 8191 10300 1493
Size: 6320 226 152 2109 1697
Fragments arranged by size:
6320 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 9058 160
Size: 872 8026 1606
Fragments arranged by size:
8026 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 10504
AvaI C'yCGr_G
Cuts at: 1870 1943 2061 2729 3114 3427 3892 3963 4694
Size: 73 118 668 385 313 465 71 731
Cuts at: 4694 4938 5402 5498 5594 7829 1870
Size: 244 464 96 96 2235 4545
Fragments arranged by size:
4545 2235 731 668 465 464 385 313
244 118 96 96 73 71
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 4130 4163 4130
Size: 33 10471
BamHI G'GATC_C
Cuts at: 7834 8201 7834
Size: 367 10137
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2106 2113 2156 2273 2314
Size: 325 217 627 319 7 43 117 41
Cuts at: 2314 2376 2475 2651 2831 2999 3256 4303 4339
Size: 62 99 176 180 168 257 1047 36
Cuts at: 4339 4679 5009 5155 6962 7825 9280 618
Size: 340 330 146 1807 863 1455 1842
Fragments arranged by size:
1842 1807 1455 1047 863 627 340 330
325 319 257 217 180 176 168 146
117 99 62 43 41 36 7
BanII G_rGCy'C
Cuts at: 1391 2312 2538 3576 3812 4268 4545 4716 4997
Size: 921 226 1038 236 456 277 171 281
Cuts at: 4997 5058 5143 5355 5410 5646 6608 7080 7823
Size: 61 85 212 55 236 962 472 743
Cuts at: 7823 1391
Size: 4072
Fragments arranged by size:
4072 1038 962 921 743 472 456 281
277 236 236 226 212 171 85 61
55
BbeI G_GCGC'C
Cuts at: 2160 2277 2380 2479 2655 2835 3003 3260 2160
Size: 117 103 99 176 180 168 257 9404
Fragments arranged by size:
9404 257 180 176 168 117 103 99
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3097 3127 3469 4617 5890 5945 6918
Size: 971 1198 30 342 1148 1273 55 973
Cuts at: 6918 7218 7316 928
Size: 300 98 4116
Fragments arranged by size:
4116 1273 1198 1148 973 971 342 300
98 55 30
BbvCI CC'TCA_GC
Cuts at: 5481 5481
Size: 10504
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5980 6014 7085 7119 8695 8729 1903
Size: 34 4043 34 1071 34 1576 34 3678
Fragments arranged by size:
4043 3678 1576 1071 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 7214 8396 9606 7214
Size: 1182 1210 8112
Fragments arranged by size:
8112 1210 1182
BclI T'GATC_A
Cuts at: 1548 1548
Size: 10504
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2300 3915 5147
Size: 107 122 71 631 605 628 1615 1232
Cuts at: 5147 6971 7592 9115 136
Size: 1824 621 1523 1525
Fragments arranged by size:
1824 1615 1525 1523 1232 631 628 621
605 122 107 71
BglII A'GATC_T
Cuts at: 3888 4404 6025 10499 3888
Size: 516 1621 4474 3893
Fragments arranged by size:
4474 3893 1621 516
BlpI GC'TnA_GC
Cuts at: 4493 5922 6003 6731 7565 7921 4493
Size: 1429 81 728 834 356 7076
Fragments arranged by size:
7076 1429 834 728 356 81
Bme1580I G_kGCm'C
Cuts at: 1790 2111 2312 2319 2902 3299 3488 3582 3678
Size: 321 201 7 583 397 189 94 96
Cuts at: 3678 3733 3749 3812 4997 5012 5143 5196 5410
Size: 55 16 63 1185 15 131 53 214
Cuts at: 5410 6810 7160 8565 9721 1790
Size: 1400 350 1405 1156 2573
Fragments arranged by size:
2573 1405 1400 1185 1156 583 397 350
321 214 201 189 131 96 94 63
55 53 16 15 7
BmgBI CAC'GTC
Cuts at: 1318 3208 3265 4789 5918 1318
Size: 1890 57 1524 1129 5904
Fragments arranged by size:
5904 1890 1524 1129 57
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4588 6811 7016
Size: 416 53 83 399 24 3171 2223 205
Cuts at: 7016 7354 8235 9193 442
Size: 338 881 958 1753
Fragments arranged by size:
3171 2223 1753 958 881 416 399 338
205 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 10504
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2183 2309 2723 3320 3755 4400 4418 5000
Size: 944 126 414 597 435 645 18 582
Cuts at: 5000 5042 5630 6089 6332 6410 6806 6844 7274
Size: 42 588 459 243 78 396 38 430
Cuts at: 7274 7718 9164 1239
Size: 444 1446 2579
Fragments arranged by size:
2579 1446 944 645 597 588 582 459
444 435 430 414 396 243 126 78
42 38 18
Bpu10I CC'TnA_GC
Cuts at: 2054 2282 4539 5411 5481 2054
Size: 228 2257 872 70 7077
Fragments arranged by size:
7077 2257 872 228 70
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2801 3491 6866 8629 9494 9792 2801
Size: 690 3375 1763 865 298 3513
Fragments arranged by size:
3513 3375 1763 865 690 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 9167 882
Size: 617 7668 2219
Fragments arranged by size:
7668 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2658 4057 4957 6136 7801 8295 493
Size: 711 1454 1399 900 1179 1665 494 2702
Fragments arranged by size:
2702 1665 1454 1399 1179 900 711 494
BsaBI GATnn'nnATC
Cuts at: 2738 5021 6518 10498 2738
Size: 2283 1497 3980 2744
Fragments arranged by size:
3980 2744 2283 1497
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2096 2157 2274 2377
Size: 53 83 186 936 563 61 117 103
Cuts at: 2377 2476 2652 2832 3000 3012 3257 3632 3711
Size: 99 176 180 168 12 245 375 79
Cuts at: 3711 6438 6615 6837 7398 7434 7559 8311 8693
Size: 2727 177 222 561 36 125 752 382
Cuts at: 8693 9328 275
Size: 635 1451
Fragments arranged by size:
2727 1451 936 752 635 563 561 382
375 245 222 186 180 177 176 168
125 117 103 99 83 79 61 53
36 12
BsaWI w'CCGG_w
Cuts at: 2663 5152 7329 7515 8937 9335 9609 9756 10167
Size: 2489 2177 186 1422 398 274 147 411
Cuts at: 10167 2663
Size: 3000
Fragments arranged by size:
3000 2489 2177 1422 411 398 274 186
147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2011 2041 2660 2690 3662
Size: 30 165 30 608 30 619 30 972
Cuts at: 3662 3692 3728 3758 4850 4880 4919 4934 4949
Size: 30 36 30 1092 30 39 15 15
Cuts at: 4949 4964 6590 6620 7424 7454 1178
Size: 15 1626 30 804 30 4228
Fragments arranged by size:
4228 1626 1092 972 804 619 608 165
39 36 30 30 30 30 30 30
30 30 30 15 15 15
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2019 4415 4871 5498 5516 5972 6253 6665
Size: 833 2396 456 627 18 456 281 412
Cuts at: 6665 7271 7478 7613 1186
Size: 606 207 135 4077
Fragments arranged by size:
4077 2396 833 627 606 456 456 412
281 207 135 18
BseYI C'CCAG_C
Cuts at: 2552 2851 3109 3601 3812 3905 5815 6199 7459
Size: 299 258 492 211 93 1910 384 1260
Cuts at: 7459 9707 10203 2552
Size: 2248 496 2853
Fragments arranged by size:
2853 2248 1910 1260 496 492 384 299
258 211 93
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2858 3311 3748 4122 4222 4392 4445
Size: 573 1122 453 437 374 100 170 53
Cuts at: 4445 5071 5107 5333 6362 6367 6849 7547 1163
Size: 626 36 226 1029 5 482 698 4120
Fragments arranged by size:
4120 1122 1029 698 626 573 482 453
437 374 226 170 100 53 36 5
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2247 2304 2496 3180 5751 6447
Size: 275 186 246 57 192 684 2571 696
Cuts at: 6447 6919 7679 7954 8715 9743 1540
Size: 472 760 275 761 1028 2301
Fragments arranged by size:
2571 2301 1028 761 760 696 684 472
275 275 246 192 186 57
BsiHKAI G_wGCw'C
Cuts at: 2370 2902 3279 3582 3678 3749 5058 5196 7119
Size: 532 377 303 96 71 1309 138 1923
Cuts at: 7119 8565 8650 9721 2370
Size: 1446 85 1071 3153
Fragments arranged by size:
3153 1923 1446 1309 1071 532 377 303
138 96 85 71
BsmI GAATG_Cn'
Cuts at: 7588 8015 8108 7588
Size: 427 93 9984
Fragments arranged by size:
9984 427 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3731 5822 6539 3731
Size: 2091 717 7696
Fragments arranged by size:
7696 2091 717
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2111 2312 2319 2370 2538 2902 3279
Size: 399 321 201 7 51 168 364 377
Cuts at: 3279 3299 3488 3576 3582 3678 3733 3749 3812
Size: 20 189 88 6 96 55 16 63
Cuts at: 3812 4268 4545 4716 4997 5012 5058 5143 5196
Size: 456 277 171 281 15 46 85 53
Cuts at: 5196 5355 5410 5646 6608 6810 7080 7119 7160
Size: 159 55 236 962 202 270 39 41
Cuts at: 7160 7823 8565 8650 9721 1391
Size: 663 742 85 1071 2174
Fragments arranged by size:
2174 1071 962 742 663 456 399 377
364 321 281 277 270 236 202 201
189 171 168 159 96 88 85 85
63 55 55 53 51 46 41 39
20 16 15 7 6
BspEI T'CCGG_A
Cuts at: 10167 10167
Size: 10504
BspHI T'CATG_A
Cuts at: 6408 8393 10123 6408
Size: 1985 1730 6789
Fragments arranged by size:
6789 1985 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3378 3523 4691 5399 5511 7461 7738 7859
Size: 2534 145 1168 708 112 1950 277 121
Cuts at: 7859 844
Size: 3489
Fragments arranged by size:
3489 2534 1950 1168 708 277 145 121
112
BsrBI CCG'CTC
Cuts at: 1394 1823 2745 3121 3142 4492 7564 8391 1394
Size: 429 922 376 21 1350 3072 827 3507
Fragments arranged by size:
3507 3072 1350 922 827 429 376 21
BsrDI GCAATG_nn'
Cuts at: 65 1248 8999 9173 65
Size: 1183 7751 174 1396
Fragments arranged by size:
7751 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2153 2663 3002 3253 3304 4773 5146
Size: 110 343 510 339 251 51 1469 373
Cuts at: 5146 6228 7092 9148 9335 1700
Size: 1082 864 2056 187 2869
Fragments arranged by size:
2869 2056 1469 1082 864 510 373 343
339 251 187 110 51
BsrGI T'GTAC_A
Cuts at: 96 6528 96
Size: 6432 4072
BssHII G'CGCG_C
Cuts at: 1925 2936 2938 2978 3230 1925
Size: 1011 2 40 252 9199
Fragments arranged by size:
9199 1011 252 40 2
BssSI C'ACGA_G
Cuts at: 2359 2645 4261 5654 8564 9576 2359
Size: 286 1616 1393 2910 1012 3287
Fragments arranged by size:
3287 2910 1616 1393 1012 286
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3240 5121 794
Size: 2446 1881 6177
Fragments arranged by size:
6177 2446 1881
BstBI TT'CG_AA
Cuts at: 2007 9308 2007
Size: 7301 3203
BstEII G'GTnAC_C
Cuts at: 1184 3986 4619 4906 1184
Size: 2802 633 287 6782
Fragments arranged by size:
6782 2802 633 287
BstXI CCAn_nnnn'nTGG
Cuts at: 5287 5342 6716 5287
Size: 55 1374 9075
Fragments arranged by size:
9075 1374 55
BstYI r'GATC_y
Cuts at: 1972 2166 2400 3888 4404 4698 5973 6025 6780
Size: 194 234 1488 516 294 1275 52 755
Cuts at: 6780 6951 7545 7834 8201 8586 8603 10055 10141
Size: 171 594 289 367 385 17 1452 86
Cuts at: 10141 10153 10499 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1488 1452 1275 755 594 516 385
367 346 294 289 234 194 171 86
52 17 12
Bsu36I CC'TnA_GG
Cuts at: 4269 5133 4269
Size: 864 9640
BtgI C'CryG_G
Cuts at: 513 1065 2022 2037 2102 2304 2372 2496 2595
Size: 552 957 15 65 202 68 124 99
Cuts at: 2595 2619 2995 3073 3159 3441 3447 4572 4671
Size: 24 376 78 86 282 6 1125 99
Cuts at: 4671 4795 4989 5026 5325 5751 5880 6744 7641
Size: 124 194 37 299 426 129 864 897
Cuts at: 7641 7710 513
Size: 69 3307
Fragments arranged by size:
3307 1125 957 897 864 552 426 376
299 282 202 194 129 124 124 99
99 86 78 69 68 65 37 24
15 6
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 5101 5785 6355 7088 7399 487
Size: 683 56 3875 684 570 733 311 3592
Fragments arranged by size:
3875 3592 733 684 683 570 311 56
BtsI GCAGTG_nn'
Cuts at: 3059 4134 4353 4829 5719 5807 6194 8020 8814
Size: 1075 219 476 890 88 387 1826 794
Cuts at: 8814 8834 3059
Size: 20 4729
Fragments arranged by size:
4729 1826 1075 890 794 476 387 219
88 20
ClaI AT'CG_AT
Cuts at: 1554 3852 1554
Size: 2298 8206
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 10469
DraI TTT'AAA
Cuts at: 7808 7808
Size: 10504
DraIII CAC_nnn'GTG
Cuts at: 4801 5311 6085 4801
Size: 510 774 9220
Fragments arranged by size:
9220 774 510
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 9511 809
Size: 507 8195 1802
Fragments arranged by size:
8195 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2244 2301 2419
Size: 54 1425 50 275 18 414 57 118
Cuts at: 2419 2493 2499 2571 2769 3177 3444 3450 3645
Size: 74 6 72 198 408 267 6 195
Cuts at: 3645 4347 4514 5238 5328 5344 5562 5748 5832
Size: 702 167 724 90 16 218 186 84
Cuts at: 5832 6444 6821 6916 6972 7593 7638 7676 7689
Size: 612 377 95 56 621 45 38 13
Cuts at: 7689 7951 8840 10469 8
Size: 262 889 1629 43
Fragments arranged by size:
1629 1425 889 724 702 621 612 414
408 377 275 267 262 218 198 195
186 167 118 95 90 84 74 72
57 56 54 50 45 43 38 18
16 13 6 6
EagI C'GGCC_G
Cuts at: 1537 1812 2244 2301 2493 3177 5748 6444 6916
Size: 275 432 57 192 684 2571 696 472
Cuts at: 6916 7676 7951 1537
Size: 760 275 4090
Fragments arranged by size:
4090 2571 760 696 684 472 432 275
275 192 57
EarI CTCTTCn'nnn_
Cuts at: 1353 2783 4469 6120 6237 7265 8434 1353
Size: 1430 1686 1651 117 1028 1169 3423
Fragments arranged by size:
3423 1686 1651 1430 1169 1028 117
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3722 4020 4105 5516 7088 9091
Size: 1064 138 2133 298 85 1411 1572 2003
Cuts at: 9091 9465 9611 387
Size: 374 146 1280
Fragments arranged by size:
2133 2003 1572 1411 1280 1064 374 298
146 138 85
EcoICRI GAG'CTC
Cuts at: 5056 5056
Size: 10504
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2183 2309 2723 3320 3422 3755 4400
Size: 393 551 126 414 597 102 333 645
Cuts at: 4400 4418 5000 5042 5630 6089 6332 6410 6806
Size: 18 582 42 588 459 243 78 396
Cuts at: 6806 6844 6978 7274 7370 7718 8567 9164 9951
Size: 38 134 296 96 348 849 597 787
Cuts at: 9951 10219 1239
Size: 268 1524
Fragments arranged by size:
1524 849 787 645 597 597 588 582
551 459 414 396 393 348 333 296
268 243 134 126 102 96 78 42
38 18
EcoNI CCTnn'n_nnAGG
Cuts at: 7187 7187
Size: 10504
EcoO109I rG'GnC_Cy
Cuts at: 2425 3153 3808 3809 3967 3974 4658 4740 4994
Size: 728 655 1 158 7 684 82 254
Cuts at: 4994 5062 5139 5140 5406 5407 6886 2425
Size: 68 77 1 266 1 1479 6043
Fragments arranged by size:
6043 1479 728 684 655 266 254 158
82 77 68 7 1 1 1
EcoRI G'AATT_C
Cuts at: 7813 8191 7813
Size: 378 10126
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2872 2872
Size: 10504
FspI TGC'GCA
Cuts at: 5102 9010 5102
Size: 3908 6596
FspAI rTGC'GCAy
Cuts at: 5102 5102
Size: 10504
HaeII r_GCGC'y
Cuts at: 1678 2061 2160 2277 2380 2479 2655 2835 2868
Size: 383 99 117 103 99 176 180 33
Cuts at: 2868 2924 3003 3260 5429 7534 9651 1678
Size: 56 79 257 2169 2105 2117 2531
Fragments arranged by size:
2531 2169 2117 2105 383 257 180 176
117 103 99 99 79 56 33
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2158 2190 3204 3236 4919
Size: 32 135 32 586 32 1014 32 1683
Cuts at: 4919 4934 4951 4966 5951 5953 5970 5983 5985
Size: 15 17 15 985 2 17 13 2
Cuts at: 5985 6002 6884 6916 7424 7456 9145 9177 9219
Size: 17 882 32 508 32 1689 32 42
Cuts at: 9219 9251 1373
Size: 32 2626
Fragments arranged by size:
2626 1689 1683 1014 985 882 586 508
135 42 32 32 32 32 32 32
32 32 17 17 17 15 15 13
2 2
HincII GTy'rAC
Cuts at: 669 1657 1938 2251 3460 3493 5731 6841 7848
Size: 988 281 313 1209 33 2238 1110 1007
Cuts at: 7848 8094 669
Size: 246 3079
Fragments arranged by size:
3079 2238 1209 1110 1007 988 313 281
246 33
HindIII A'AGCT_T
Cuts at: 748 6926 748
Size: 6178 4326
HpaI GTT'AAC
Cuts at: 8094 8094
Size: 10504
KasI G'GCGC_C
Cuts at: 2156 2273 2376 2475 2651 2831 2999 3256 2156
Size: 117 103 99 176 180 168 257 9404
Fragments arranged by size:
9404 257 180 176 168 117 103 99
KpnI G_GTAC'C
Cuts at: 7829 7829
Size: 10504
MfeI C'AATT_G
Cuts at: 8103 8103
Size: 10504
MluI A'CGCG_T
Cuts at: 2586 2910 10380 2586
Size: 324 7470 2710
Fragments arranged by size:
7470 2710 324
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2826 2907 4251 4525 4710 4833 7308 9618
Size: 1521 81 1344 274 185 123 2475 2310
Cuts at: 9618 9802 1305
Size: 184 2007
Fragments arranged by size:
2475 2310 2007 1521 1344 274 185 184
123 81
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2771 3452 3647 4349 6974
Size: 54 1425 343 939 681 195 702 2625
Cuts at: 6974 7640 10
Size: 666 2874
Fragments arranged by size:
2874 2625 1425 939 702 681 666 343
195 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3413 3440 3533 3923 4218 4337 5031
Size: 648 2247 27 93 390 295 119 694
Cuts at: 5031 5285 5840 6524 6902 7634 8462 8821 8980
Size: 254 555 684 378 732 828 359 159
Cuts at: 8980 518
Size: 2042
Fragments arranged by size:
2247 2042 828 732 694 684 648 555
390 378 359 295 254 159 119 93
27
MspA1I CmG'CkG
Cuts at: 1606 2039 2104 2119 2266 2329 2347 2374 2410
Size: 433 65 15 147 63 18 27 36
Cuts at: 2410 2470 2621 3075 3161 3349 3382 3473 3819
Size: 60 151 454 86 188 33 91 346
Cuts at: 3819 4170 5512 5761 5815 6534 7459 7920 8597
Size: 351 1342 249 54 719 925 461 677
Cuts at: 8597 9745 9990 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1342 1148 925 719 677 461 454
433 351 346 249 245 188 151 147
91 86 65 63 60 54 36 33
27 18 15
NaeI GCC'GGC
Cuts at: 2155 3004 4775 5148 6230 2155
Size: 849 1771 373 1082 6429
Fragments arranged by size:
6429 1771 1082 849 373
NarI GG'CG_CC
Cuts at: 2157 2274 2377 2476 2652 2832 3000 3257 2157
Size: 117 103 99 176 180 168 257 9404
Fragments arranged by size:
9404 257 180 176 168 117 103 99
NcoI C'CATG_G
Cuts at: 513 1065 2022 4572 4671 4989 5026 5325 6744
Size: 552 957 2550 99 318 37 299 1419
Cuts at: 6744 7641 513
Size: 897 3376
Fragments arranged by size:
3376 2550 1419 957 897 552 318 299
99 37
NdeI CA'TA_TG
Cuts at: 387 387
Size: 10504
NgoMIV G'CCGG_C
Cuts at: 2153 3002 4773 5146 6228 2153
Size: 849 1771 373 1082 6429
Fragments arranged by size:
6429 1771 1082 849 373
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 10504
NotI GC'GGCC_GC
Cuts at: 7951 7951
Size: 10504
NspI r_CATG'y
Cuts at: 1132 1229 2560 3532 4633 5221 6529 7862 9407
Size: 97 1331 972 1101 588 1308 1333 1545
Cuts at: 9407 1132
Size: 2229
Fragments arranged by size:
2229 1545 1333 1331 1308 1101 972 588
97
PasI CC'CwG_GG
Cuts at: 1348 3148 5740 6163 1348
Size: 1800 2592 423 5689
Fragments arranged by size:
5689 2592 1800 423
PciI A'CATG_T
Cuts at: 9403 9403
Size: 10504
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2428 3643 4600 4978 5065 6310 1108
Size: 1320 1215 957 378 87 1245 5302
Fragments arranged by size:
5302 1320 1245 1215 957 378 87
PfoI T'CCnGG_A
Cuts at: 4527 6066 6783 7119 7251 7548 4527
Size: 1539 717 336 132 297 7483
Fragments arranged by size:
7483 1539 717 336 297 132
PmeI GTTT'AAAC
Cuts at: 7808 7808
Size: 10504
PmlI CAC'GTG
Cuts at: 4057 4057
Size: 10504
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2011 2043 8573 8605 10086 10118 2011
Size: 32 6530 32 1481 32 2397
Fragments arranged by size:
6530 2397 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 3153 3967 4740 5062 6886 3153
Size: 814 773 322 1824 6771
Fragments arranged by size:
6771 1824 814 773 322
PshAI GACnn'nnGTC
Cuts at: 1613 4617 6767 1613
Size: 3004 2150 5350
Fragments arranged by size:
5350 3004 2150
PsiI TTA'TAA
Cuts at: 7656 8074 7656
Size: 418 10086
PspOMI G'GGCC_C
Cuts at: 2308 3808 4993 5139 5406 2308
Size: 1500 1185 146 267 7406
Fragments arranged by size:
7406 1500 1185 267 146
PstI C_TGCA'G
Cuts at: 830 2409 3375 3396 3888 4130 4668 5814 5861
Size: 1579 966 21 492 242 538 1146 47
Cuts at: 5861 5952 6012 7458 7734 7856 830
Size: 91 60 1446 276 122 3478
Fragments arranged by size:
3478 1579 1446 1146 966 538 492 276
242 122 91 60 47 21
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 10504
PvuII CAG'CTG
Cuts at: 2266 2347 2410 2470 3349 4170 5761 5815 6534
Size: 81 63 60 879 821 1591 54 719
Cuts at: 6534 7459 2266
Size: 925 5311
Fragments arranged by size:
5311 1591 925 879 821 719 81 63
60 54
SacI G_AGCT'C
Cuts at: 5058 5058
Size: 10504
SacII CC_GC'GG
Cuts at: 2040 2105 2375 2622 3076 3162 2040
Size: 65 270 247 454 86 9382
Fragments arranged by size:
9382 454 270 247 86 65
SalI G'TCGA_C
Cuts at: 1936 6839 7846 1936
Size: 4903 1007 4594
Fragments arranged by size:
4903 4594 1007
SanDI GG'GwC_CC
Cuts at: 3153 3967 4740 3153
Size: 814 773 8917
Fragments arranged by size:
8917 814 773
SapI GCTCTTCn'nnn_
Cuts at: 2783 4469 6120 2783
Size: 1686 1651 7167
Fragments arranged by size:
7167 1686 1651
SbfI CC_TGCA'GG
Cuts at: 7856 7856
Size: 10504
ScaI AGT'ACT
Cuts at: 6395 7238 8752 10464 6395
Size: 843 1514 1712 6435
Fragments arranged by size:
6435 1712 1514 843
SexAI A'CCwGG_T
Cuts at: 4449 4449
Size: 10504
SfcI C'TryA_G
Cuts at: 826 1045 2405 3085 3371 3392 3884 4126 4664
Size: 219 1360 680 286 21 492 242 538
Cuts at: 4664 5810 5857 5948 6008 7454 7730 7786 7852
Size: 1146 47 91 60 1446 276 56 66
Cuts at: 7852 8987 9668 9859 826
Size: 1135 681 191 1471
Fragments arranged by size:
1471 1446 1360 1146 1135 681 680 538
492 286 276 242 219 191 91 66
60 56 47 21
SfiI GGCCn_nnn'nGGCC
Cuts at: 2300 2300
Size: 10504
SfoI GGC'GCC
Cuts at: 2158 2275 2378 2477 2653 2833 3001 3258 2158
Size: 117 103 99 176 180 168 257 9404
Fragments arranged by size:
9404 257 180 176 168 117 103 99
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 10504
SgrAI Cr'CCGG_yG
Cuts at: 1700 3002 1700
Size: 1302 9202
SmaI CCC'GGG
Cuts at: 1872 2063 3116 3429 4940 5404 5596 7831 1872
Size: 191 1053 313 1511 464 192 2235 4545
Fragments arranged by size:
4545 2235 1511 1053 464 313 192 191
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2816 3506 6845 8608 9509 9771
Size: 197 926 873 690 3339 1763 901 262
Cuts at: 9771 820
Size: 1553
Fragments arranged by size:
3339 1763 1553 926 901 873 690 262
197
SnaBI TAC'GTA
Cuts at: 493 7801 8295 493
Size: 7308 494 2702
Fragments arranged by size:
7308 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 10504
SphI G_CATG'C
Cuts at: 1132 2560 3532 7862 1132
Size: 1428 972 4330 3774
Fragments arranged by size:
4330 3774 1428 972
SrfI GCCC'GGGC
Cuts at: 2063 2063
Size: 10504
SspI AAT'ATT
Cuts at: 5 52 8428 5
Size: 47 8376 2081
Fragments arranged by size:
8376 2081 47
StuI AGG'CCT
Cuts at: 1517 2357 4247 6791 7424 7796 1517
Size: 840 1890 2544 633 372 4225
Fragments arranged by size:
4225 2544 1890 840 633 372
StyI C'CwwG_G
Cuts at: 513 1065 2022 2835 4572 4671 4989 4998 5026
Size: 552 957 813 1737 99 318 9 28
Cuts at: 5026 5325 6033 6744 7152 7497 7641 7943 513
Size: 299 708 711 408 345 144 302 3074
Fragments arranged by size:
3074 1737 957 813 711 708 552 408
345 318 302 299 144 99 28 9
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3091 3689 4411 5716 6207 7327 8531
Size: 1255 1511 598 722 1305 491 1120 1204
Cuts at: 8531 8548 8701 8886 9331 325
Size: 17 153 185 445 1498
Fragments arranged by size:
1511 1498 1305 1255 1204 1120 722 598
491 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 6393 6528 7236
Size: 274 80 33 51 1443 4416 135 708
Cuts at: 7236 8750 10462 96
Size: 1514 1712 138
Fragments arranged by size:
4416 1712 1514 1443 708 274 138 135
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3368 3400 3728 3760 4798 4830 4850 4882 10464
Size: 32 328 32 1038 32 20 32 5582
Cuts at: 10464 10496 3368
Size: 32 3376
Fragments arranged by size:
5582 3376 1038 328 32 32 32 32
32 20
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 10504
XbaI T'CTAG_A
Cuts at: 7840 7840
Size: 10504
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2284 2746 4552 5812 7639 2284
Size: 462 1806 1260 1827 5149
Fragments arranged by size:
5149 1827 1806 1260 462
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 10504
XmaI C'CCGG_G
Cuts at: 1870 2061 3114 3427 4938 5402 5594 7829 1870
Size: 191 1053 313 1511 464 192 2235 4545
Fragments arranged by size:
4545 2235 1511 1053 464 313 192 191
XmnI GAAnn'nnTTC
Cuts at: 7040 7622 8633 7040
Size: 582 1011 8911
Fragments arranged by size:
8911 1011 582
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3633 6838 8312 276
Size: 53 83 186 936 2099 3205 1474 2468
Fragments arranged by size:
3205 2468 2099 1474 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI
BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI
BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI FspAI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NotI NspI PasI
PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI
PsiI PspOMI PstI PvuI PvuII SacI SacII SalI
SanDI SapI SbfI ScaI SexAI SfcI SfiI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AscI AvrII BfrBI BplI BsiWI BstZ17I EcoRV FseI
NruI NsiI PacI PspXI PsrI RsrII SwaI