(Circular) (Six-base) MAPSORT of: pFN21ASDA0613.seq Check: 8572 from: 1 to: 6910 pFN21ASDA0613 6910 bp With 182 enzymes: SgfI * January 16, 2009 15:30 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3001 3163 3424 3926 4109 3001 Size: 162 261 502 183 5802 Fragments arranged by size: 5802 502 261 183 162 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4720 278 Size: 53 83 186 936 3184 2468 Fragments arranged by size: 3184 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2739 2757 3243 4253 1937 Size: 802 18 486 1010 4594 Fragments arranged by size: 4594 1010 802 486 18 Acc65I G'GTAC_C Cuts at: 4231 4231 Size: 6910 AclI AA'CG_TT Cuts at: 1563 3807 5037 5410 1563 Size: 2244 1230 373 3063 Fragments arranged by size: 3063 2244 1230 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3132 3759 4973 6357 6625 1632 Size: 1500 627 1214 1384 268 1917 Fragments arranged by size: 1917 1500 1384 1214 627 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6713 AflIII A'CryG_T Cuts at: 1203 5809 6786 1203 Size: 4606 977 1327 Fragments arranged by size: 4606 1327 977 AgeI A'CCGG_T Cuts at: 5741 5741 Size: 6910 AhdI GACnn_n'nnGTC Cuts at: 5639 5639 Size: 6910 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6910 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3799 3855 1184 Size: 2615 56 4239 Fragments arranged by size: 4239 2615 56 AlwNI CAG_nnn'CTG Cuts at: 3041 3080 3155 3257 3562 4653 6225 3041 Size: 39 75 102 305 1091 1572 3726 Fragments arranged by size: 3726 1572 1091 305 102 75 39 ApaI G_GGCC'C Cuts at: 2052 2058 2554 3185 3464 3704 2052 Size: 6 496 631 279 240 5258 Fragments arranged by size: 5258 631 496 279 240 6 ApaLI G'TGCA_C Cuts at: 2944 4967 6123 2944 Size: 2023 1156 3731 Fragments arranged by size: 3731 2023 1156 ApoI r'AATT_y Cuts at: 1493 2876 4219 4445 4597 6706 1493 Size: 1383 1343 226 152 2109 1697 Fragments arranged by size: 2109 1697 1383 1343 226 152 AseI AT'TA_AT Cuts at: 160 1032 5464 160 Size: 872 4432 1606 Fragments arranged by size: 4432 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6910 AvaI C'yCGr_G Cuts at: 1870 1943 2051 2553 2587 2689 3207 3526 3567 Size: 73 108 502 34 102 518 319 41 Cuts at: 3567 4235 1870 Size: 668 4545 Fragments arranged by size: 4545 668 518 502 319 108 102 73 41 34 BamHI G'GATC_C Cuts at: 4240 4607 4240 Size: 367 6543 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2377 2414 2423 3604 3732 Size: 325 217 627 590 37 9 1181 128 Cuts at: 3732 4231 5686 618 Size: 499 1455 1842 Fragments arranged by size: 1842 1455 1181 627 590 499 325 217 128 37 9 BanII G_rGCy'C Cuts at: 1391 2052 2058 2436 2554 2588 3185 3464 3704 Size: 661 6 378 118 34 597 279 240 Cuts at: 3704 3987 4229 1391 Size: 283 242 4072 Fragments arranged by size: 4072 661 597 378 283 279 242 240 118 34 6 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3981 928 Size: 971 2082 3857 Fragments arranged by size: 3857 2082 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5101 5135 1903 Size: 34 3164 34 3678 Fragments arranged by size: 3678 3164 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4802 6012 4802 Size: 1210 5700 BclI T'GATC_A Cuts at: 1548 1548 Size: 6910 BfrBI ATG'CAT Cuts at: 3889 3889 Size: 6910 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5521 136 Size: 107 122 71 631 605 3849 1525 Fragments arranged by size: 3849 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6905 6905 Size: 6910 BlpI GC'TnA_GC Cuts at: 2150 3639 4327 2150 Size: 1489 688 4733 Fragments arranged by size: 4733 1489 688 Bme1580I G_kGCm'C Cuts at: 1790 2052 2058 2554 2815 2948 3185 3464 3704 Size: 262 6 496 261 133 237 279 240 Cuts at: 3704 4971 6127 1790 Size: 1267 1156 2573 Fragments arranged by size: 2573 1267 1156 496 279 262 261 240 237 133 6 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6910 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2055 2134 3003 Size: 416 53 83 399 24 638 79 869 Cuts at: 3003 4641 5599 442 Size: 1638 958 1753 Fragments arranged by size: 1753 1638 958 869 638 416 399 83 79 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6910 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2732 2732 Size: 6910 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2289 4154 5570 1239 Size: 1050 1865 1416 2579 Fragments arranged by size: 2579 1865 1416 1050 Bpu10I CC'TnA_GC Cuts at: 2435 2435 Size: 6910 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2269 3916 5035 5900 6198 2269 Size: 1647 1119 865 298 2981 Fragments arranged by size: 2981 1647 1119 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2630 5573 882 Size: 617 1131 2943 2219 Fragments arranged by size: 2943 2219 1131 617 BsaAI yAC'GTr Cuts at: 493 1204 4207 4701 493 Size: 711 3003 494 2702 Fragments arranged by size: 3003 2702 711 494 BsaBI GATnn'nnATC Cuts at: 6904 6904 Size: 6910 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2184 4717 5099 5734 Size: 53 83 186 936 651 2533 382 635 Cuts at: 5734 275 Size: 1451 Fragments arranged by size: 2533 1451 936 651 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5343 5741 6015 6162 6573 5343 Size: 398 274 147 411 5680 Fragments arranged by size: 5680 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2287 2317 3299 3323 3329 Size: 30 165 30 884 30 982 24 6 Cuts at: 3329 3353 3367 3397 1178 Size: 24 14 30 4691 Fragments arranged by size: 4691 982 884 165 30 30 30 30 24 24 14 6 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2470 1186 Size: 1284 5626 BseYI C'CCAG_C Cuts at: 2145 2381 2387 2985 3391 3577 3589 3649 6113 Size: 236 6 598 406 186 12 60 2464 Cuts at: 6113 6609 2145 Size: 496 2446 Fragments arranged by size: 2464 2446 598 496 406 236 186 60 12 6 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3180 3420 1163 Size: 573 1444 240 4653 Fragments arranged by size: 4653 1444 573 240 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4360 5121 6149 1540 Size: 275 186 2359 761 1028 2301 Fragments arranged by size: 2359 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2436 2948 3539 4971 5056 6127 2436 Size: 512 591 1432 85 1071 3219 Fragments arranged by size: 3219 1432 1071 591 512 85 BsmI GAATG_Cn' Cuts at: 4421 4514 4421 Size: 93 6817 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2052 2058 2436 2554 2588 2815 2948 Size: 399 262 6 378 118 34 227 133 Cuts at: 2948 3185 3464 3539 3704 3987 4229 4971 5056 Size: 237 279 75 165 283 242 742 85 Cuts at: 5056 6127 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 399 378 283 279 262 242 237 227 165 133 118 85 75 34 6 BspEI T'CCGG_A Cuts at: 6573 6573 Size: 6910 BspHI T'CATG_A Cuts at: 4799 6529 4799 Size: 1730 5180 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2838 3001 3163 3424 3926 4109 4265 844 Size: 1994 163 162 261 502 183 156 3489 Fragments arranged by size: 3489 1994 502 261 183 163 162 156 BsrBI CCG'CTC Cuts at: 1394 1823 2351 4797 1394 Size: 429 528 2446 3507 Fragments arranged by size: 3507 2446 528 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5405 5579 65 Size: 1183 4157 174 1396 Fragments arranged by size: 4157 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2323 2938 5554 5741 1700 Size: 110 513 615 2616 187 2869 Fragments arranged by size: 2869 2616 615 513 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6910 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6910 BssSI C'ACGA_G Cuts at: 2165 4970 5982 2165 Size: 2805 1012 3093 Fragments arranged by size: 3093 2805 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 3562 4026 794 Size: 2768 464 3678 Fragments arranged by size: 3678 2768 464 BstBI TT'CG_AA Cuts at: 2007 5714 2007 Size: 3707 3203 BstEII G'GTnAC_C Cuts at: 1184 2159 1184 Size: 975 5935 BstXI CCAn_nnnn'nTGG Cuts at: 3874 3874 Size: 6910 BstYI r'GATC_y Cuts at: 1972 4240 4607 4992 5009 6461 6547 6559 6905 Size: 2268 367 385 17 1452 86 12 346 Cuts at: 6905 1972 Size: 1977 Fragments arranged by size: 2268 1977 1452 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2394 2525 2642 3600 2394 Size: 131 117 958 5704 Fragments arranged by size: 5704 958 131 117 BtgI C'CryG_G Cuts at: 513 1065 2513 3717 4030 4047 513 Size: 552 1448 1204 313 17 3376 Fragments arranged by size: 3376 1448 1204 552 313 17 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3040 3835 487 Size: 683 56 1814 795 3562 Fragments arranged by size: 3562 1814 795 683 56 BtsI GCAGTG_nn' Cuts at: 3048 3141 3219 3679 4426 5220 5240 3048 Size: 93 78 460 747 794 20 4718 Fragments arranged by size: 4718 794 747 460 93 78 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6910 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6875 DraI TTT'AAA Cuts at: 4214 4214 Size: 6910 DraIII CAC_nnn'GTG Cuts at: 4026 4026 Size: 6910 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5917 809 Size: 507 4601 1802 Fragments arranged by size: 4601 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2172 3030 3138 Size: 54 1425 50 275 18 342 858 108 Cuts at: 3138 4357 5246 6875 8 Size: 1219 889 1629 43 Fragments arranged by size: 1629 1425 1219 889 858 342 275 108 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4357 1537 Size: 275 2545 4090 Fragments arranged by size: 4090 2545 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3124 3151 3735 3789 3968 4840 1353 Size: 1771 27 584 54 179 872 3423 Fragments arranged by size: 3423 1771 872 584 179 54 27 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2461 3052 5497 5871 6017 387 Size: 1064 138 872 591 2445 374 146 1280 Fragments arranged by size: 2445 1280 1064 872 591 374 146 138 EcoICRI GAG'CTC Cuts at: 2434 2434 Size: 6910 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2289 3132 3759 4154 4973 5570 6357 Size: 393 657 843 627 395 819 597 787 Cuts at: 6357 6625 1239 Size: 268 1524 Fragments arranged by size: 1524 843 819 787 657 627 597 395 393 268 EcoO109I rG'GnC_Cy Cuts at: 2055 2344 2550 2557 2728 3108 3181 3182 3460 Size: 289 206 7 171 380 73 1 278 Cuts at: 3460 3461 3596 3700 2055 Size: 1 135 104 5265 Fragments arranged by size: 5265 380 289 278 206 171 135 104 73 7 1 1 EcoRI G'AATT_C Cuts at: 2876 4219 4597 2876 Size: 1343 378 5189 Fragments arranged by size: 5189 1343 378 FspI TGC'GCA Cuts at: 4114 5416 4114 Size: 1302 5608 HaeII r_GCGC'y Cuts at: 1678 2748 6057 1678 Size: 1070 3309 2531 Fragments arranged by size: 3309 2531 1070 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2287 2319 2732 2764 3494 Size: 32 135 32 715 32 413 32 730 Cuts at: 3494 3526 5551 5583 5625 5657 1373 Size: 32 2025 32 42 32 2626 Fragments arranged by size: 2626 2025 730 715 413 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2188 4254 4500 669 Size: 988 281 250 2066 246 3079 Fragments arranged by size: 3079 2066 988 281 250 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6910 HpaI GTT'AAC Cuts at: 4500 4500 Size: 6910 KpnI G_GTAC'C Cuts at: 4235 4235 Size: 6910 MfeI C'AATT_G Cuts at: 4509 4509 Size: 6910 MluI A'CGCG_T Cuts at: 6786 6786 Size: 6910 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2677 2850 6024 6208 1305 Size: 1372 173 3174 184 2007 Fragments arranged by size: 3174 2007 1372 184 173 MscI TGG'CCA Cuts at: 10 64 1489 1832 2174 3032 10 Size: 54 1425 343 342 858 3888 Fragments arranged by size: 3888 1425 858 343 342 54 MslI CAynn'nnrTG Cuts at: 518 1166 4868 5227 5386 518 Size: 648 3702 359 159 2042 Fragments arranged by size: 3702 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2515 2968 3038 3456 3593 3847 4326 5003 Size: 909 453 70 418 137 254 479 677 Cuts at: 5003 6151 6396 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1148 909 677 479 453 418 254 245 137 70 NcoI C'CATG_G Cuts at: 513 1065 3717 4030 513 Size: 552 2652 313 3393 Fragments arranged by size: 3393 2652 552 313 NdeI CA'TA_TG Cuts at: 387 387 Size: 6910 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6910 NotI GC'GGCC_GC Cuts at: 4357 4357 Size: 6910 NsiI A_TGCA'T Cuts at: 3891 3891 Size: 6910 NspI r_CATG'y Cuts at: 1132 1229 2098 2936 3893 4268 5813 1132 Size: 97 869 838 957 375 1545 2229 Fragments arranged by size: 2229 1545 957 869 838 375 97 PasI CC'CwG_GG Cuts at: 1348 2058 3281 3489 4078 1348 Size: 710 1223 208 589 4180 Fragments arranged by size: 4180 1223 710 589 208 PciI A'CATG_T Cuts at: 5809 5809 Size: 6910 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6910 PfoI T'CCnGG_A Cuts at: 2111 2111 Size: 6910 PmeI GTTT'AAAC Cuts at: 4214 4214 Size: 6910 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4979 5011 6492 6524 4979 Size: 32 1481 32 5365 Fragments arranged by size: 5365 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2344 2557 2728 3596 2344 Size: 213 171 868 5658 Fragments arranged by size: 5658 868 213 171 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6910 PsiI TTA'TAA Cuts at: 4480 4480 Size: 6910 PspOMI G'GGCC_C Cuts at: 2048 2054 2550 3181 3460 3700 2048 Size: 6 496 631 279 240 5258 Fragments arranged by size: 5258 631 496 279 240 6 PspXI vC'TCGA_Gb Cuts at: 2587 2587 Size: 6910 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 3794 3826 3794 Size: 32 6878 PstI C_TGCA'G Cuts at: 830 2076 2265 2835 4262 830 Size: 1246 189 570 1427 3478 Fragments arranged by size: 3478 1427 1246 570 189 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6910 PvuII CAG'CTG Cuts at: 3038 3038 Size: 6910 SacI G_AGCT'C Cuts at: 2436 2436 Size: 6910 SacII CC_GC'GG Cuts at: 2516 2516 Size: 6910 SalI G'TCGA_C Cuts at: 1936 4252 1936 Size: 2316 4594 SapI GCTCTTCn'nnn_ Cuts at: 3789 3789 Size: 6910 SbfI CC_TGCA'GG Cuts at: 4262 4262 Size: 6910 ScaI AGT'ACT Cuts at: 5158 6870 5158 Size: 1712 5198 SfcI C'TryA_G Cuts at: 826 1045 2072 2261 2831 3124 3172 3268 3451 Size: 219 1027 189 570 293 48 96 183 Cuts at: 3451 4258 5393 6074 6265 826 Size: 807 1135 681 191 1471 Fragments arranged by size: 1471 1135 1027 807 681 570 293 219 191 189 183 96 48 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6910 SgrAI Cr'CCGG_yG Cuts at: 1700 2938 1700 Size: 1238 5672 SmaI CCC'GGG Cuts at: 1872 2053 2555 3209 3528 3569 4237 1872 Size: 181 502 654 319 41 668 4545 Fragments arranged by size: 4545 668 654 502 319 181 41 SmlI C'TyrA_G Cuts at: 820 1017 1943 2248 2587 3895 5014 5915 6177 Size: 197 926 305 339 1308 1119 901 262 Cuts at: 6177 820 Size: 1553 Fragments arranged by size: 1553 1308 1119 926 901 339 305 262 197 SnaBI TAC'GTA Cuts at: 493 4207 4701 493 Size: 3714 494 2702 Fragments arranged by size: 3714 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 6910 SphI G_CATG'C Cuts at: 1132 2936 3893 4268 1132 Size: 1804 957 375 3774 Fragments arranged by size: 3774 1804 957 375 SrfI GCCC'GGGC Cuts at: 2053 2053 Size: 6910 SspI AAT'ATT Cuts at: 5 52 4834 5 Size: 47 4782 2081 Fragments arranged by size: 4782 2081 47 StuI AGG'CCT Cuts at: 1517 2495 2687 3116 1517 Size: 978 192 429 5311 Fragments arranged by size: 5311 978 429 192 StyI C'CwwG_G Cuts at: 513 1065 3104 3717 4030 4349 513 Size: 552 2039 613 313 319 3074 Fragments arranged by size: 3074 2039 613 552 319 313 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2395 3176 3214 3481 4937 4954 5107 Size: 1255 815 781 38 267 1456 17 153 Cuts at: 5107 5292 5737 325 Size: 185 445 1498 Fragments arranged by size: 1498 1456 1255 815 781 445 267 185 153 38 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2625 5156 6868 Size: 274 80 33 51 1443 648 2531 1712 Cuts at: 6868 96 Size: 138 Fragments arranged by size: 2531 1712 1443 648 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2292 2324 3367 3399 6870 6902 2292 Size: 32 1043 32 3471 32 2300 Fragments arranged by size: 3471 2300 1043 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2183 1316 Size: 867 6043 XbaI T'CTAG_A Cuts at: 4246 4246 Size: 6910 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2206 2206 Size: 6910 XhoI C'TCGA_G Cuts at: 1943 2587 1943 Size: 644 6266 XmaI C'CCGG_G Cuts at: 1870 2051 2553 3207 3526 3567 4235 1870 Size: 181 502 654 319 41 668 4545 Fragments arranged by size: 4545 668 654 502 319 181 41 XmnI GAAnn'nnTTC Cuts at: 5039 5039 Size: 6910 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4718 276 Size: 53 83 186 936 3184 2468 Fragments arranged by size: 3184 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PstI PvuI PvuII SacI SacII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BbvCI BsiWI BsmBI BstZ17I EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI RsrII SanDI SexAI SfiI SfoI SwaI