(Circular) (Six-base) MAPSORT of: pFN21ASDA0607.seq Check: 4223 from: 1 to: 6865 pFN21ASDA0607 6865 bp With 182 enzymes: SgfI * January 16, 2009 15:30 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2156 3103 3283 2156 Size: 947 180 5738 Fragments arranged by size: 5738 947 180 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3626 4675 278 Size: 53 83 186 936 2090 1049 2468 Fragments arranged by size: 2468 2090 1049 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4208 1937 Size: 2271 4594 Acc65I G'GTAC_C Cuts at: 4186 4186 Size: 6865 AclI AA'CG_TT Cuts at: 1563 4992 5365 1563 Size: 3429 373 3063 Fragments arranged by size: 3429 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2837 3713 4928 6312 6580 1632 Size: 1205 876 1215 1384 268 1917 Fragments arranged by size: 1917 1384 1215 1205 876 268 AfeI AGC'GCT Cuts at: 2084 2084 Size: 6865 AflII C'TTAA_G Cuts at: 820 1017 3038 820 Size: 197 2021 4647 Fragments arranged by size: 4647 2021 197 AflIII A'CryG_T Cuts at: 1203 5764 6741 1203 Size: 4561 977 1327 Fragments arranged by size: 4561 1327 977 AgeI A'CCGG_T Cuts at: 5696 5696 Size: 6865 AhdI GACnn_n'nnGTC Cuts at: 5594 5594 Size: 6865 AleI CACnn'nnGTG Cuts at: 1166 2954 3494 1166 Size: 1788 540 4537 Fragments arranged by size: 4537 1788 540 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2505 2860 1184 Size: 1321 355 5189 Fragments arranged by size: 5189 1321 355 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3866 3898 3866 Size: 32 6833 AlwNI CAG_nnn'CTG Cuts at: 2446 2557 2701 3034 3223 3970 4147 4608 6180 Size: 111 144 333 189 747 177 461 1572 Cuts at: 6180 2446 Size: 3131 Fragments arranged by size: 3131 1572 747 461 333 189 177 144 111 ApaI G_GGCC'C Cuts at: 3333 3402 3430 3333 Size: 69 28 6768 Fragments arranged by size: 6768 69 28 ApaLI G'TGCA_C Cuts at: 4922 6078 4922 Size: 1156 5709 ApoI r'AATT_y Cuts at: 1493 4174 4400 4552 6661 1493 Size: 2681 226 152 2109 1697 Fragments arranged by size: 2681 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 5419 160 Size: 872 4387 1606 Fragments arranged by size: 4387 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6865 AvaI C'yCGr_G Cuts at: 1870 1943 2388 3402 3747 3808 4013 4190 1870 Size: 73 445 1014 345 61 205 177 4545 Fragments arranged by size: 4545 1014 445 345 205 177 73 61 BamHI G'GATC_C Cuts at: 2859 3003 3162 4195 4562 2859 Size: 144 159 1033 367 5162 Fragments arranged by size: 5162 1033 367 159 144 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2251 2481 2498 3170 3375 Size: 325 217 627 464 230 17 672 205 Cuts at: 3375 4186 5641 618 Size: 811 1455 1842 Fragments arranged by size: 1842 1455 811 672 627 464 325 230 217 205 17 BanII G_rGCy'C Cuts at: 1391 2098 2619 2691 2795 3141 3333 3402 3430 Size: 707 521 72 104 346 192 69 28 Cuts at: 3430 3693 3815 3879 4158 4184 1391 Size: 263 122 64 279 26 4072 Fragments arranged by size: 4072 707 521 346 279 263 192 122 104 72 69 64 28 26 BbeI G_GCGC'C Cuts at: 2255 2255 Size: 6865 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5894 BbvCI CC'TCA_GC Cuts at: 2667 2667 Size: 6865 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2111 2145 2710 2744 5056 5090 1903 Size: 34 174 34 565 34 2312 34 3678 Fragments arranged by size: 3678 2312 565 174 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4757 5967 4757 Size: 1210 5655 BclI T'GATC_A Cuts at: 1548 3414 3576 1548 Size: 1866 162 4837 Fragments arranged by size: 4837 1866 162 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2303 2765 2834 Size: 107 122 71 631 605 631 462 69 Cuts at: 2834 3089 3179 3272 3305 3337 5476 136 Size: 255 90 93 33 32 2139 1525 Fragments arranged by size: 2139 1525 631 631 605 462 255 122 107 93 90 71 69 33 32 BglII A'GATC_T Cuts at: 6860 6860 Size: 6865 BlpI GC'TnA_GC Cuts at: 2789 4282 2789 Size: 1493 5372 Bme1580I G_kGCm'C Cuts at: 1790 2535 2733 2951 3333 3402 3430 4926 6082 Size: 745 198 218 382 69 28 1496 1156 Cuts at: 6082 1790 Size: 2573 Fragments arranged by size: 2573 1496 1156 745 382 218 198 69 28 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6865 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4596 5554 442 Size: 416 53 83 399 24 3179 958 1753 Fragments arranged by size: 3179 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 3833 1056 Size: 2777 4088 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3208 3368 3208 Size: 160 6705 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2099 2957 3705 3959 5525 1239 Size: 860 858 748 254 1566 2579 Fragments arranged by size: 2579 1566 860 858 748 254 Bpu10I CC'TnA_GC Cuts at: 2667 3710 2667 Size: 1043 5822 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4990 5855 6153 4990 Size: 865 298 5702 Fragments arranged by size: 5702 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3182 5528 882 Size: 617 1683 2346 2219 Fragments arranged by size: 2346 2219 1683 617 BsaAI yAC'GTr Cuts at: 493 1204 3793 4162 4656 493 Size: 711 2589 369 494 2702 Fragments arranged by size: 2702 2589 711 494 369 BsaBI GATnn'nnATC Cuts at: 3413 6859 3413 Size: 3446 3419 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2252 3588 3623 4672 Size: 53 83 186 936 719 1336 35 1049 Cuts at: 4672 5054 5689 275 Size: 382 635 1451 Fragments arranged by size: 1451 1336 1049 936 719 635 382 186 83 53 35 BsaWI w'CCGG_w Cuts at: 5298 5696 5970 6117 6528 5298 Size: 398 274 147 411 5635 Fragments arranged by size: 5635 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3866 3896 1178 Size: 30 165 30 2463 30 4147 Fragments arranged by size: 4147 2463 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2954 3200 3275 3284 3350 3683 4118 1186 Size: 1768 246 75 9 66 333 435 3933 Fragments arranged by size: 3933 1768 435 333 246 75 66 9 BseYI C'CCAG_C Cuts at: 3288 6068 6564 3288 Size: 2780 496 3589 Fragments arranged by size: 3589 2780 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2796 3077 3662 1163 Size: 573 1060 281 585 4366 Fragments arranged by size: 4366 1060 585 573 281 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4315 5076 6104 1540 Size: 275 186 2314 761 1028 2301 Fragments arranged by size: 2314 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2691 2795 3879 4926 5011 6082 2691 Size: 104 1084 1047 85 1071 3474 Fragments arranged by size: 3474 1084 1071 1047 104 85 BsmI GAATG_Cn' Cuts at: 2744 3017 4376 4469 2744 Size: 273 1359 93 5140 Fragments arranged by size: 5140 1359 273 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2237 2936 3339 4100 2237 Size: 699 403 761 5002 Fragments arranged by size: 5002 761 699 403 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2098 2535 2619 2691 2733 2795 2951 Size: 399 308 437 84 72 42 62 156 Cuts at: 2951 3141 3333 3402 3430 3693 3815 3879 4158 Size: 190 192 69 28 263 122 64 279 Cuts at: 4158 4184 4926 5011 6082 1391 Size: 26 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 437 399 308 279 263 192 190 156 122 85 84 72 69 64 62 42 28 26 BspEI T'CCGG_A Cuts at: 6528 6528 Size: 6865 BspHI T'CATG_A Cuts at: 3072 3618 4754 6484 3072 Size: 546 1136 1730 3453 Fragments arranged by size: 3453 1730 1136 546 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2156 2631 3103 3114 3283 3539 3876 4220 Size: 1312 475 472 11 169 256 337 344 Cuts at: 4220 844 Size: 3489 Fragments arranged by size: 3489 1312 475 472 344 337 256 169 11 BsrBI CCG'CTC Cuts at: 1394 1823 2418 4752 1394 Size: 429 595 2334 3507 Fragments arranged by size: 3507 2334 595 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2487 5360 5534 65 Size: 1183 1239 2873 174 1396 Fragments arranged by size: 2873 1396 1239 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3905 4065 5509 5696 1700 Size: 110 2095 160 1444 187 2869 Fragments arranged by size: 2869 2095 1444 187 160 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6865 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6865 BssSI C'ACGA_G Cuts at: 3062 3554 4925 5937 3062 Size: 492 1371 1012 3990 Fragments arranged by size: 3990 1371 1012 492 BstAPI GCAn_nnn'nTGC Cuts at: 794 3034 3970 794 Size: 2240 936 3689 Fragments arranged by size: 3689 2240 936 BstBI TT'CG_AA Cuts at: 2007 5669 2007 Size: 3662 3203 BstEII G'GTnAC_C Cuts at: 1184 2971 3559 1184 Size: 1787 588 4490 Fragments arranged by size: 4490 1787 588 BstYI r'GATC_y Cuts at: 1972 2196 2749 2859 3003 3162 3408 4195 4562 Size: 224 553 110 144 159 246 787 367 Cuts at: 4562 4947 4964 6416 6502 6514 6860 1972 Size: 385 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 787 553 385 367 346 246 224 159 144 110 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2022 513 Size: 552 957 5356 Fragments arranged by size: 5356 957 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6126 Fragments arranged by size: 6126 683 56 BtsI GCAGTG_nn' Cuts at: 2323 3032 3467 3831 3994 4148 4381 5175 5195 Size: 709 435 364 163 154 233 794 20 Cuts at: 5195 2323 Size: 3993 Fragments arranged by size: 3993 794 709 435 364 233 163 154 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6865 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6830 DraI TTT'AAA Cuts at: 4169 4169 Size: 6865 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5872 809 Size: 507 4556 1802 Fragments arranged by size: 4556 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2331 2628 3180 Size: 54 1425 50 275 18 501 297 552 Cuts at: 3180 3297 3306 3338 3481 3678 3959 4023 4110 Size: 117 9 32 143 197 281 64 87 Cuts at: 4110 4312 5201 6830 8 Size: 202 889 1629 43 Fragments arranged by size: 1629 1425 889 552 501 297 281 275 202 197 143 117 87 64 54 50 43 32 18 9 EagI C'GGCC_G Cuts at: 1537 1812 4312 1537 Size: 275 2500 4090 Fragments arranged by size: 4090 2500 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3732 3779 4795 1353 Size: 2379 47 1016 3423 Fragments arranged by size: 3423 2379 1016 47 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2209 2627 5452 5826 5972 387 Size: 1064 138 620 418 2825 374 146 1280 Fragments arranged by size: 2825 1280 1064 620 418 374 146 138 EcoICRI GAG'CTC Cuts at: 2689 2793 3877 2689 Size: 104 1084 5677 Fragments arranged by size: 5677 1084 104 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2099 2837 2957 3705 3713 3959 4928 Size: 393 467 738 120 748 8 246 969 Cuts at: 4928 5525 6312 6580 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 969 787 748 738 597 467 393 268 246 120 8 EcoNI CCTnn'n_nnAGG Cuts at: 2102 2516 2648 3110 3257 3416 2102 Size: 414 132 462 147 159 5551 Fragments arranged by size: 5551 462 414 159 147 132 EcoO109I rG'GnC_Cy Cuts at: 2395 2474 2554 2641 3329 3399 3426 3427 2395 Size: 79 80 87 688 70 27 1 5833 Fragments arranged by size: 5833 688 87 80 79 70 27 1 EcoRI G'AATT_C Cuts at: 4174 4552 4174 Size: 378 6487 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3157 3157 Size: 6865 FspI TGC'GCA Cuts at: 5371 5371 Size: 6865 HaeII r_GCGC'y Cuts at: 1678 2086 2255 6012 1678 Size: 408 169 3757 2531 Fragments arranged by size: 3757 2531 408 169 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3179 3211 5506 5538 5580 Size: 32 135 32 1607 32 2295 32 42 Cuts at: 5580 5612 1373 Size: 32 2626 Fragments arranged by size: 2626 2295 1607 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4209 4455 669 Size: 988 281 2271 246 3079 Fragments arranged by size: 3079 2271 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6865 HpaI GTT'AAC Cuts at: 4455 4455 Size: 6865 KasI G'GCGC_C Cuts at: 2251 2251 Size: 6865 KpnI G_GTAC'C Cuts at: 4190 4190 Size: 6865 MfeI C'AATT_G Cuts at: 4464 4464 Size: 6865 MluI A'CGCG_T Cuts at: 6741 6741 Size: 6865 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2877 5979 6163 1305 Size: 1572 3102 184 2007 Fragments arranged by size: 3102 2007 1572 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3299 3308 3340 3483 3680 Size: 54 1425 343 1467 9 32 143 197 Cuts at: 3680 10 Size: 3195 Fragments arranged by size: 3195 1467 1425 343 197 143 54 32 9 MslI CAynn'nnrTG Cuts at: 518 1166 2538 2954 3494 3617 4823 5182 5341 Size: 648 1372 416 540 123 1206 359 159 Cuts at: 5341 518 Size: 2042 Fragments arranged by size: 2042 1372 1206 648 540 416 359 159 123 MspA1I CmG'CkG Cuts at: 1606 2698 3220 3764 3941 3957 3979 4281 4958 Size: 1092 522 544 177 16 22 302 677 Cuts at: 4958 6106 6351 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1148 1092 677 544 522 302 245 177 22 16 NarI GG'CG_CC Cuts at: 2252 2252 Size: 6865 NcoI C'CATG_G Cuts at: 513 1065 2022 513 Size: 552 957 5356 Fragments arranged by size: 5356 957 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6865 NheI G'CTAG_C Cuts at: 1052 3829 1052 Size: 2777 4088 NotI GC'GGCC_GC Cuts at: 4312 4312 Size: 6865 NspI r_CATG'y Cuts at: 1132 1229 3895 4223 5768 1132 Size: 97 2666 328 1545 2229 Fragments arranged by size: 2666 2229 1545 328 97 PasI CC'CwG_GG Cuts at: 1348 3326 3685 1348 Size: 1978 359 4528 Fragments arranged by size: 4528 1978 359 PciI A'CATG_T Cuts at: 5764 5764 Size: 6865 PflMI CCAn_nnn'nTGG Cuts at: 1108 2557 3295 1108 Size: 1449 738 4678 Fragments arranged by size: 4678 1449 738 PfoI T'CCnGG_A Cuts at: 2876 3006 2876 Size: 130 6735 PmeI GTTT'AAAC Cuts at: 4169 4169 Size: 6865 PmlI CAC'GTG Cuts at: 3793 3793 Size: 6865 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3866 3898 4934 4966 6447 6479 3866 Size: 32 1036 32 1481 32 4252 Fragments arranged by size: 4252 1481 1036 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2395 2395 Size: 6865 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6865 PsiI TTA'TAA Cuts at: 4435 4435 Size: 6865 PspOMI G'GGCC_C Cuts at: 3329 3398 3426 3329 Size: 69 28 6768 Fragments arranged by size: 6768 69 28 PstI C_TGCA'G Cuts at: 830 2318 2757 3114 3459 3528 3873 4095 4217 Size: 1488 439 357 345 69 345 222 122 Cuts at: 4217 830 Size: 3478 Fragments arranged by size: 3478 1488 439 357 345 345 222 122 69 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6865 PvuII CAG'CTG Cuts at: 2698 3220 3764 3979 2698 Size: 522 544 215 5584 Fragments arranged by size: 5584 544 522 215 SacI G_AGCT'C Cuts at: 2691 2795 3879 2691 Size: 104 1084 5677 Fragments arranged by size: 5677 1084 104 SalI G'TCGA_C Cuts at: 1936 4207 1936 Size: 2271 4594 SanDI GG'GwC_CC Cuts at: 2395 2395 Size: 6865 SapI GCTCTTCn'nnn_ Cuts at: 3732 3732 Size: 6865 SbfI CC_TGCA'GG Cuts at: 3873 4217 3873 Size: 344 6521 ScaI AGT'ACT Cuts at: 5113 6825 5113 Size: 1712 5153 SfcI C'TryA_G Cuts at: 826 1045 2314 2454 2753 3110 3455 3524 3869 Size: 219 1269 140 299 357 345 69 345 Cuts at: 3869 4091 4213 5348 6029 6220 826 Size: 222 122 1135 681 191 1471 Fragments arranged by size: 1471 1269 1135 681 357 345 345 299 222 219 191 140 122 69 SfiI GGCCn_nnn'nGGCC Cuts at: 2303 3272 3305 3337 2303 Size: 969 33 32 5831 Fragments arranged by size: 5831 969 33 32 SfoI GGC'GCC Cuts at: 2253 2253 Size: 6865 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6865 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6865 SmaI CCC'GGG Cuts at: 1872 2390 3404 3810 4192 1872 Size: 518 1014 406 382 4545 Fragments arranged by size: 4545 1014 518 406 382 SmlI C'TyrA_G Cuts at: 820 1017 1943 3038 4969 5870 6132 820 Size: 197 926 1095 1931 901 262 1553 Fragments arranged by size: 1931 1553 1095 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4162 4656 493 Size: 3669 494 2702 Fragments arranged by size: 3669 2702 494 SpeI A'CTAG_T Cuts at: 152 3637 152 Size: 3485 3380 SphI G_CATG'C Cuts at: 1132 3895 4223 1132 Size: 2763 328 3774 Fragments arranged by size: 3774 2763 328 SspI AAT'ATT Cuts at: 5 52 4789 5 Size: 47 4737 2081 Fragments arranged by size: 4737 2081 47 StuI AGG'CCT Cuts at: 1517 3899 1517 Size: 2382 4483 StyI C'CwwG_G Cuts at: 513 1065 2022 2151 2244 4134 4304 513 Size: 552 957 129 93 1890 170 3074 Fragments arranged by size: 3074 1890 957 552 170 129 93 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2279 2482 3158 4892 4909 5062 5247 Size: 1255 699 203 676 1734 17 153 185 Cuts at: 5247 5692 325 Size: 445 1498 Fragments arranged by size: 1734 1498 1255 699 676 445 203 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 5111 6823 96 Size: 274 80 33 51 1443 3134 1712 138 Fragments arranged by size: 3134 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3154 3186 6825 6857 3154 Size: 32 3639 32 3162 Fragments arranged by size: 3639 3162 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6865 XbaI T'CTAG_A Cuts at: 4201 4201 Size: 6865 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3333 3333 Size: 6865 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6865 XmaI C'CCGG_G Cuts at: 1870 2388 3402 3808 4190 1870 Size: 518 1014 406 382 4545 Fragments arranged by size: 4545 1014 518 406 382 XmnI GAAnn'nnTTC Cuts at: 4994 4994 Size: 6865 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3624 4673 276 Size: 53 83 186 936 2090 1049 2468 Fragments arranged by size: 2468 2090 1049 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SanDI SapI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AscI AvrII BaeI BfrBI BsiWI BstXI BstZ17I Bsu36I DraIII EcoRV FseI FspAI NaeI NgoMIV NruI NsiI PacI PspXI PsrI RsrII SacII SexAI SrfI SwaI