Restriction map for
pFN21ASDA0592
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# NO CUT ENZYMES ( 94 enzymes) #
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AatII Acc65I AccIII AclI AflII
AflIII AgeI Alw44I Aor51HI ApaI
ApaLI AscI AseI AsnI Asp718I
AviII AvrII BamHI BbrPI BclI
BfrI BglI BlnI BlpI Bpu1102I
BsaAI BsaI BseAI BsiWI BsmBI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrGI BssHII Bst1107I BstAPI BstBI
BstXI BstZ17I CelII ClaI CpoI
DraI DrdI Eco47III EcoT22I FbaI
FseI FspI HindIII KpnI KspI
MfeI MluI MroI MunI NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PstI
PvuI RcaI RsrII SacII SalI
SbfI SexAI SfiI SfuI SgrAI
SnaBI SpeI SphI Sse8387I SspBI
StuI SwaI XbaI XhoI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AccI 3050 GTAGAC GT^[AC][GT]AC
AccII 120 CGCG CG^CG
AhdI 1912 GACAGCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI 1912 GACAGCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 1773 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 2953 CCCGGG C^[CT]CG[GA]G
BalI 3168 TGGCCA TGG^CCA
BbeI 3151 GGCGCC GGCGC^C
BcgI 2484 CGATCCTGATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 3842 GTATCC GTATCC(6/5)
BsaWI 8 ACCGGA [AT]^CCGG[AT]
BsoBI 2953 CCCGGG C^[CT]CG[GA]G
BspMI 1916 GCAGGT ACCTGC(4/8)
BsrBI 58 GAGCGG CCGCTC(-3/-3)
BssSI 362 CACGAG CACGAG(-5/-1)
BstEII 1548 GGTTACC G^GT[ACGT]ACC
BstPI 1548 GGTTACC G^GT[ACGT]ACC
BstUI 120 CGCG CG^CG
CfoI 3152 GCGC GCG^C
DraIII 3910 CACAAGGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 1912 GACAGCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 1548 GGTTACC G^GT[ACGT]ACC
EcoRI 145 GAATTC G^AATTC
HaeII 3151 GGCGCC [GA]GCGC^[CT]
HhaI 3152 GCGC GCG^C
HinP1I 3152 GCGC G^CGC
HpaI 2728 GTTAAC GTT^AAC
KasI 3151 GGCGCC G^GCGCC
MaeII 1702 ACGT A^CGT
MluNI 3168 TGGCCA TGG^CCA
MscI 3168 TGGCCA TGG^CCA
MvnI 120 CGCG CG^CG
NaeI 36 GCCGGC GCC^GGC
NarI 3151 GGCGCC GG^CGCC
NdeI 2787 CATATG CA^TATG
NgoMIV 36 GCCGGC G^CCGGC
PflFI 1773 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
SacI 1819 GAGCTC GAGCT^C
SapI 2527 GAAGAGC GCTCTTC(1/4)
ScaI 321 AGTACT AGT^ACT
SfoI 3151 GGCGCC GGC^GCC
SmaI 2953 CCCGGG CCC^GGG
SspI 1879 AATATT AAT^ATT
TaqI 909 TCGA T^CGA
Tth111I 1773 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 909 TCGA T^CGA
XmaCI 2953 CCCGGG C^CCGGG
XmaI 2953 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 30 enzymes) #
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AcyI 39 GGCGTC G[GA]^CG[CT]C
3151 GGCGCC
AlwNI 25 CAGGAGCTG CAG[ACGT][ACGT][ACGT]^CTG
1217 CAGGAGCTG
AocI 938 CCTCAGG CC^T[ACGT]AGG
2806 CCTCAGG
Asp700I 989 GAACTCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2758 GAATCCATTC
AspHI 1819 GAGCTC G[AT]GC[AT]^C
2505 GAGCAC
BbvCI 331 GCTGAGG CCTCAGC(-5/-2)
3103 GCTGAGG
BsaBI 2650 GATGATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3478 GATGATGATC
BsaHI 39 GGCGTC G[GA]^CG[CT]C
3151 GGCGCC
BsiEI 61 CGGCCG CG[GA][CT]^CG
113 CGGCCG
BsiHKAI 1819 GAGCTC G[AT]GC[AT]^C
2505 GAGCAC
BsmI 3942 GAATGC GAATGC(1/-1)
2334 GCATTC
BsrDI 2025 CATTGC GCAATG(2/0)
3132 CATTGC
BsrFI 36 GCCGGC [GA]^CCGG[CT]
2968 GCCGGT
Bsu36I 938 CCTCAGG CC^T[ACGT]AGG
2806 CCTCAGG
Cfr10I 36 GCCGGC [GA]^CCGG[CT]
2968 GCCGGT
EagI 61 CGGCCG C^GGCCG
113 CGGCCG
EclXI 61 CGGCCG C^GGCCG
113 CGGCCG
Eco52I 61 CGGCCG C^GGCCG
113 CGGCCG
Eco81I 938 CCTCAGG CC^T[ACGT]AGG
2806 CCTCAGG
EcoNI 3241 CCTTGGGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3454 CCTGCTCTAGG
HgaI 118 GACGC GACGC(5/10)
40 GCGTC
Hin1I 39 GGCGTC G[GA]^CG[CT]C
3151 GGCGCC
HincII 2575 GTTGAC GT[CT]^[GA]AC
2728 GTTAAC
HindII 2575 GTTGAC GT[CT]^[GA]AC
2728 GTTAAC
HphI 1366 GGTGA GGTGA(8/7)
1450 GGTGA
MamI 2650 GATGATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3478 GATGATGATC
NcoI 902 CCATGG C^CATGG
2362 CCATGG
PvuII 1757 CAGCTG CAG^CTG
3227 CAGCTG
SmlI 325 CTCAAG C^T[CT][GA]AG
1573 CTCAAG
XmnI 989 GAACTCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2758 GAATCCATTC
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# 3 OR MORE CUT ENZYMES ( 86 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AvaII BanI BanII BbsI
BbvI BcnI BfaI BglII BmyI
BpmI BpuAI BsaJI BseRI BsgI
BsiYI BslI BsmAI BsmFI Bsp1286I
BsrI BssKI BstF5I BstNI BstYI
Cac8I Cfr13I DdeI DpnII DraII
DsaI EaeI EarI EcoO109I EcoRII
EcoRV EcoT14I FauI Fnu4HI FokI
HaeIII HapII HinfI HpaII ItaI
Ksp632I MaeI MaeIII MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV PflMI PleI PpuMI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI StyI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI Van91I XcmI
XhoII