(Circular) (Six-base) MAPSORT of: pFN21ASDA0592.seq Check: 3721 from: 1 to: 8689 pFN21ASDA0592 8689 bp With 182 enzymes: SgfI * January 16, 2009 15:30 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 6499 278 Size: 53 83 186 936 4963 2468 Fragments arranged by size: 4963 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 5074 6032 1937 Size: 3137 958 4594 Fragments arranged by size: 4594 3137 958 Acc65I G'GTAC_C Cuts at: 6010 6010 Size: 8689 AclI AA'CG_TT Cuts at: 1563 6816 7189 1563 Size: 5253 373 3063 Fragments arranged by size: 5253 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3003 3323 4911 6752 8136 8404 1632 Size: 1371 320 1588 1841 1384 268 1917 Fragments arranged by size: 1917 1841 1588 1384 1371 320 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 8492 AflIII A'CryG_T Cuts at: 1203 7588 8565 1203 Size: 6385 977 1327 Fragments arranged by size: 6385 1327 977 AgeI A'CCGG_T Cuts at: 7520 7520 Size: 8689 AhdI GACnn_n'nnGTC Cuts at: 3940 7418 3940 Size: 3478 5211 AleI CACnn'nnGTG Cuts at: 1166 3272 1166 Size: 2106 6583 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 5103 1184 Size: 3919 4770 AlwNI CAG_nnn'CTG Cuts at: 2053 3245 6432 8004 2053 Size: 1192 3187 1572 2738 Fragments arranged by size: 3187 2738 1572 1192 ApaLI G'TGCA_C Cuts at: 6746 7902 6746 Size: 1156 7533 ApoI r'AATT_y Cuts at: 1493 2168 4283 4837 5998 6224 6376 8485 1493 Size: 675 2115 554 1161 226 152 2109 1697 Fragments arranged by size: 2115 2109 1697 1161 675 554 226 152 AseI AT'TA_AT Cuts at: 160 1032 7243 160 Size: 872 6211 1606 Fragments arranged by size: 6211 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8689 AvaI C'yCGr_G Cuts at: 1870 1943 4976 6014 1870 Size: 73 3033 1038 4545 Fragments arranged by size: 4545 3033 1038 73 BamHI G'GATC_C Cuts at: 6019 6386 6019 Size: 367 8322 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2056 3415 4186 4613 5174 Size: 325 217 627 269 1359 771 427 561 Cuts at: 5174 6010 7465 618 Size: 836 1455 1842 Fragments arranged by size: 1842 1455 1359 836 771 627 561 427 325 269 217 BanII G_rGCy'C Cuts at: 1391 2073 3846 5468 6008 1391 Size: 682 1773 1622 540 4072 Fragments arranged by size: 4072 1773 1622 682 540 BbeI G_GCGC'C Cuts at: 5178 5178 Size: 8689 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2260 2387 2990 3198 4029 4076 4104 Size: 971 361 127 603 208 831 47 28 Cuts at: 4104 4531 5232 928 Size: 427 701 4385 Fragments arranged by size: 4385 971 831 701 603 427 361 208 127 47 28 BbvCI CC'TCA_GC Cuts at: 2355 5127 2355 Size: 2772 5917 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4496 4530 6880 6914 1903 Size: 34 2559 34 2350 34 3678 Fragments arranged by size: 3678 2559 2350 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5876 6581 7791 5876 Size: 705 1210 6774 Fragments arranged by size: 6774 1210 705 BclI T'GATC_A Cuts at: 1548 1548 Size: 8689 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 7300 136 Size: 107 122 71 631 605 5628 1525 Fragments arranged by size: 5628 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2404 3487 4334 4884 8684 2404 Size: 1083 847 550 3800 2409 Fragments arranged by size: 3800 2409 1083 847 550 BlpI GC'TnA_GC Cuts at: 6106 6106 Size: 8689 Bme1580I G_kGCm'C Cuts at: 1790 5378 6750 7906 1790 Size: 3588 1372 1156 2573 Fragments arranged by size: 3588 2573 1372 1156 BmgBI CAC'GTC Cuts at: 1318 3726 1318 Size: 2408 6281 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2293 6420 7378 Size: 416 53 83 399 24 876 4127 958 Cuts at: 7378 442 Size: 1753 Fragments arranged by size: 4127 1753 958 876 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 8689 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3091 3667 4103 4195 3091 Size: 576 436 92 7585 Fragments arranged by size: 7585 576 436 92 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3111 3204 3959 5307 7349 1239 Size: 1872 93 755 1348 2042 2579 Fragments arranged by size: 2579 2042 1872 1348 755 93 Bpu10I CC'TnA_GC Cuts at: 2355 3321 4341 5016 5109 5127 2355 Size: 966 1020 675 93 18 5917 Fragments arranged by size: 5917 1020 966 675 93 18 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2333 3581 6814 7679 7977 2333 Size: 1248 3233 865 298 3045 Fragments arranged by size: 3233 3045 1248 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 7352 882 Size: 617 5853 2219 Fragments arranged by size: 5853 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 5986 6480 493 Size: 711 4782 494 2702 Fragments arranged by size: 4782 2702 711 494 BsaBI GATnn'nnATC Cuts at: 4677 5505 8683 4677 Size: 828 3178 4683 Fragments arranged by size: 4683 3178 828 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2063 5175 6496 6878 Size: 53 83 186 936 530 3112 1321 382 Cuts at: 6878 7513 275 Size: 635 1451 Fragments arranged by size: 3112 1451 1321 936 635 530 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2031 7122 7520 7794 7941 8352 2031 Size: 5091 398 274 147 411 2368 Fragments arranged by size: 5091 2368 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2060 2072 2090 2102 2483 Size: 30 165 30 657 12 18 12 381 Cuts at: 2483 2513 3177 3207 3881 3911 4936 4966 5840 Size: 30 664 30 674 30 1025 30 874 Cuts at: 5840 5870 1178 Size: 30 3997 Fragments arranged by size: 3997 1025 874 674 664 657 381 165 30 30 30 30 30 30 30 18 12 12 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2114 2351 2717 2720 2840 2957 3155 3215 Size: 928 237 366 3 120 117 198 60 Cuts at: 3215 3659 3902 4079 4125 5252 5848 1186 Size: 444 243 177 46 1127 596 4027 Fragments arranged by size: 4027 1127 928 596 444 366 243 237 198 177 120 117 60 46 3 BseYI C'CCAG_C Cuts at: 3235 4701 4852 7892 8388 3235 Size: 1466 151 3040 496 3536 Fragments arranged by size: 3536 3040 1466 496 151 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 5109 5244 5886 1163 Size: 573 3373 135 642 3966 Fragments arranged by size: 3966 3373 642 573 135 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2087 2139 6139 6900 7928 1540 Size: 275 186 86 52 4000 761 1028 2301 Fragments arranged by size: 4000 2301 1028 761 275 186 86 52 BsiHKAI G_wGCw'C Cuts at: 3846 4532 6750 6835 7906 3846 Size: 686 2218 85 1071 4629 Fragments arranged by size: 4629 2218 1071 686 85 BsmI GAATG_Cn' Cuts at: 4357 5971 6200 6293 4357 Size: 1614 229 93 6753 Fragments arranged by size: 6753 1614 229 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2073 3846 4532 5378 5468 6008 6750 Size: 399 283 1773 686 846 90 540 742 Cuts at: 6750 6835 7906 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1773 1071 846 742 686 540 399 283 90 85 BspEI T'CCGG_A Cuts at: 8352 8352 Size: 8689 BspHI T'CATG_A Cuts at: 6578 8308 6578 Size: 1730 6959 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3930 6044 844 Size: 3086 2114 3489 Fragments arranged by size: 3489 3086 2114 BsrBI CCG'CTC Cuts at: 1394 1823 2083 6576 1394 Size: 429 260 4493 3507 Fragments arranged by size: 4493 3507 429 260 BsrDI GCAATG_nn' Cuts at: 65 1248 4047 5154 7184 7358 65 Size: 1183 2799 1107 2030 174 1396 Fragments arranged by size: 2799 2030 1396 1183 1107 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2059 4991 7333 7520 1700 Size: 110 249 2932 2342 187 2869 Fragments arranged by size: 2932 2869 2342 249 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 8689 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 8689 BssSI C'ACGA_G Cuts at: 2385 6749 7761 2385 Size: 4364 1012 3313 Fragments arranged by size: 4364 3313 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 8689 BstBI TT'CG_AA Cuts at: 2007 7493 2007 Size: 5486 3203 BstEII G'GTnAC_C Cuts at: 1184 3571 1184 Size: 2387 6302 BstYI r'GATC_y Cuts at: 1972 2103 2404 2551 3487 4334 4884 5381 6019 Size: 131 301 147 936 847 550 497 638 Cuts at: 6019 6386 6771 6788 8240 8326 8338 8684 1972 Size: 367 385 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 936 847 638 550 497 385 367 346 301 147 131 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2962 4830 2962 Size: 1868 6821 BtgI C'CryG_G Cuts at: 513 1065 2087 2925 4385 4948 5370 5391 513 Size: 552 1022 838 1460 563 422 21 3811 Fragments arranged by size: 3811 1460 1022 838 563 552 422 21 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3498 4221 487 Size: 683 56 2272 723 4955 Fragments arranged by size: 4955 2272 723 683 56 BtsI GCAGTG_nn' Cuts at: 2665 3456 3886 5503 6205 6999 7019 2665 Size: 791 430 1617 702 794 20 4335 Fragments arranged by size: 4335 1617 794 791 702 430 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 8689 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 8654 DraI TTT'AAA Cuts at: 5993 5993 Size: 8689 DraIII CAC_nnn'GTG Cuts at: 5938 5938 Size: 8689 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 7696 809 Size: 507 6380 1802 Fragments arranged by size: 6380 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2084 2136 5191 Size: 54 1425 50 275 18 254 52 3055 Cuts at: 5191 5198 6136 7025 8654 8 Size: 7 938 889 1629 43 Fragments arranged by size: 3055 1629 1425 938 889 275 254 54 52 50 43 18 7 EagI C'GGCC_G Cuts at: 1537 1812 2084 2136 6136 1537 Size: 275 272 52 4000 4090 Fragments arranged by size: 4090 4000 275 272 52 EarI CTCTTCn'nnn_ Cuts at: 1353 2685 2694 2775 3024 3158 3750 3816 4209 Size: 1332 9 81 249 134 592 66 393 Cuts at: 4209 4437 4545 6619 1353 Size: 228 108 2074 3423 Fragments arranged by size: 3423 2074 1332 592 393 249 228 134 108 81 66 9 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 7276 7650 7796 387 Size: 1064 138 5687 374 146 1280 Fragments arranged by size: 5687 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3844 3844 Size: 8689 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3003 3111 3204 3323 3959 4911 5307 Size: 393 1371 108 93 119 636 952 396 Cuts at: 5307 6752 7349 8136 8404 1239 Size: 1445 597 787 268 1524 Fragments arranged by size: 1524 1445 1371 952 787 636 597 396 393 268 119 108 93 EcoNI CCTnn'n_nnAGG Cuts at: 5268 5481 5268 Size: 213 8476 EcoO109I rG'GnC_Cy Cuts at: 2193 3969 5150 5242 2193 Size: 1776 1181 92 5640 Fragments arranged by size: 5640 1776 1181 92 EcoRI G'AATT_C Cuts at: 2168 5998 6376 2168 Size: 3830 378 4481 Fragments arranged by size: 4481 3830 378 EcoRV GAT'ATC Cuts at: 2479 3466 5764 2479 Size: 987 2298 5404 Fragments arranged by size: 5404 2298 987 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3592 4055 4574 3592 Size: 463 519 7707 Fragments arranged by size: 7707 519 463 FspI TGC'GCA Cuts at: 7195 7195 Size: 8689 HaeII r_GCGC'y Cuts at: 1678 5178 7836 1678 Size: 3500 2658 2531 Fragments arranged by size: 3500 2658 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2693 2725 3194 3226 3395 Size: 32 135 32 1121 32 469 32 169 Cuts at: 3395 3427 3787 3819 3926 3958 4936 4968 4990 Size: 32 360 32 107 32 978 32 22 Cuts at: 4990 5022 5603 5635 5840 5872 7330 7362 7404 Size: 32 581 32 205 32 1458 32 42 Cuts at: 7404 7436 1373 Size: 32 2626 Fragments arranged by size: 2626 1458 1121 978 581 469 360 205 169 135 107 42 32 32 32 32 32 32 32 32 32 32 32 32 32 22 HincII GTy'rAC Cuts at: 669 1657 1938 4600 4753 6033 6279 669 Size: 988 281 2662 153 1280 246 3079 Fragments arranged by size: 3079 2662 1280 988 281 246 153 HindIII A'AGCT_T Cuts at: 748 748 Size: 8689 HpaI GTT'AAC Cuts at: 4753 6279 4753 Size: 1526 7163 KasI G'GCGC_C Cuts at: 5174 5174 Size: 8689 KpnI G_GTAC'C Cuts at: 6014 6014 Size: 8689 MfeI C'AATT_G Cuts at: 6288 6288 Size: 8689 MluI A'CGCG_T Cuts at: 8565 8565 Size: 8689 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2046 5967 7803 7987 1305 Size: 741 3921 1836 184 2007 Fragments arranged by size: 3921 2007 1836 741 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 5193 10 Size: 54 1425 343 3361 3506 Fragments arranged by size: 3506 3361 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2733 2765 3272 4384 6647 7006 7165 Size: 648 1567 32 507 1112 2263 359 159 Cuts at: 7165 518 Size: 2042 Fragments arranged by size: 2263 2042 1567 1112 648 507 359 159 32 MspA1I CmG'CkG Cuts at: 1606 3782 4856 4874 5252 5258 6105 6782 7930 Size: 2176 1074 18 378 6 847 677 1148 Cuts at: 7930 8175 1606 Size: 245 2120 Fragments arranged by size: 2176 2120 1148 1074 847 677 378 245 18 6 NaeI GCC'GGC Cuts at: 2061 2061 Size: 8689 NarI GG'CG_CC Cuts at: 5175 5175 Size: 8689 NcoI C'CATG_G Cuts at: 513 1065 2925 4385 513 Size: 552 1860 1460 4817 Fragments arranged by size: 4817 1860 1460 552 NdeI CA'TA_TG Cuts at: 387 4811 387 Size: 4424 4265 NgoMIV G'CCGG_C Cuts at: 2059 2059 Size: 8689 NheI G'CTAG_C Cuts at: 1052 1052 Size: 8689 NotI GC'GGCC_GC Cuts at: 6136 6136 Size: 8689 NspI r_CATG'y Cuts at: 1132 1229 6047 7592 1132 Size: 97 4818 1545 2229 Fragments arranged by size: 4818 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 3957 1348 Size: 2609 6080 PciI A'CATG_T Cuts at: 7588 7588 Size: 8689 PflMI CCAn_nnn'nTGG Cuts at: 1108 2053 4646 4708 4954 5450 1108 Size: 945 2593 62 246 496 4347 Fragments arranged by size: 4347 2593 945 496 246 62 PfoI T'CCnGG_A Cuts at: 2229 2421 4542 5562 2229 Size: 192 2121 1020 5356 Fragments arranged by size: 5356 2121 1020 192 PmeI GTTT'AAAC Cuts at: 5993 5993 Size: 8689 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 6758 6790 8271 8303 6758 Size: 32 1481 32 7144 Fragments arranged by size: 7144 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2193 3969 5150 2193 Size: 1776 1181 5732 Fragments arranged by size: 5732 1776 1181 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 8689 PsiI TTA'TAA Cuts at: 6259 6259 Size: 8689 PstI C_TGCA'G Cuts at: 830 6041 830 Size: 5211 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8689 PvuII CAG'CTG Cuts at: 3782 5252 3782 Size: 1470 7219 SacI G_AGCT'C Cuts at: 3846 3846 Size: 8689 SalI G'TCGA_C Cuts at: 1936 6031 1936 Size: 4095 4594 SanDI GG'GwC_CC Cuts at: 3969 3969 Size: 8689 SapI GCTCTTCn'nnn_ Cuts at: 4545 4545 Size: 8689 SbfI CC_TGCA'GG Cuts at: 6041 6041 Size: 8689 ScaI AGT'ACT Cuts at: 2346 6937 8649 2346 Size: 4591 1712 2386 Fragments arranged by size: 4591 2386 1712 SfcI C'TryA_G Cuts at: 826 1045 2153 2162 2652 6037 7172 7853 8044 Size: 219 1108 9 490 3385 1135 681 191 Cuts at: 8044 826 Size: 1471 Fragments arranged by size: 3385 1471 1135 1108 681 490 219 191 9 SfoI GGC'GCC Cuts at: 5176 5176 Size: 8689 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8689 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 8689 SmaI CCC'GGG Cuts at: 1872 4978 6016 1872 Size: 3106 1038 4545 Fragments arranged by size: 4545 3106 1038 SmlI C'TyrA_G Cuts at: 820 1017 1943 2348 3596 6793 7694 7956 820 Size: 197 926 405 1248 3197 901 262 1553 Fragments arranged by size: 3197 1553 1248 926 901 405 262 197 SnaBI TAC'GTA Cuts at: 493 5986 6480 493 Size: 5493 494 2702 Fragments arranged by size: 5493 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 8689 SphI G_CATG'C Cuts at: 1132 6047 1132 Size: 4915 3774 SspI AAT'ATT Cuts at: 5 52 3904 6613 5 Size: 47 3852 2709 2081 Fragments arranged by size: 3852 2709 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 8689 StyI C'CwwG_G Cuts at: 513 1065 2925 3335 3990 4053 4269 4385 5264 Size: 552 1860 410 655 63 216 116 879 Cuts at: 5264 5409 6128 513 Size: 145 719 3074 Fragments arranged by size: 3074 1860 879 719 655 552 410 216 145 116 63 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3077 5438 6716 6733 6886 7071 7516 Size: 1255 1497 2361 1278 17 153 185 445 Cuts at: 7516 325 Size: 1498 Fragments arranged by size: 2361 1498 1497 1278 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2344 6935 8647 Size: 274 80 33 51 1443 367 4591 1712 Cuts at: 8647 96 Size: 138 Fragments arranged by size: 4591 1712 1443 367 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2060 2072 2092 2104 3432 3464 3881 3913 4132 Size: 12 20 12 1328 32 417 32 219 Cuts at: 4132 4164 5924 5956 8649 8681 2060 Size: 32 1760 32 2693 32 2068 Fragments arranged by size: 2693 2068 1760 1328 417 219 32 32 32 32 32 20 12 12 Tth111I GACn'n_nGTC Cuts at: 1316 3799 1316 Size: 2483 6206 XbaI T'CTAG_A Cuts at: 6025 6025 Size: 8689 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2932 5443 5809 2932 Size: 2511 366 5812 Fragments arranged by size: 5812 2511 366 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 8689 XmaI C'CCGG_G Cuts at: 1870 4976 6014 1870 Size: 3106 1038 4545 Fragments arranged by size: 4545 3106 1038 XmnI GAAnn'nnTTC Cuts at: 3016 4785 6818 3016 Size: 1769 2033 4887 Fragments arranged by size: 4887 2033 1769 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 6497 276 Size: 53 83 186 936 4963 2468 Fragments arranged by size: 4963 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BfrBI BsiWI BsmBI BstXI BstZ17I FseI FspAI NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacII SexAI SfiI SrfI SwaI