(Circular) (Six-base) MAPSORT of: pFN21ASDA0557.seq Check: 1589 from: 1 to: 6169 pFN21ASDA0557 6169 bp With 182 enzymes: SgfI * January 16, 2009 15:30 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3008 3344 3008 Size: 336 5833 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3035 3979 278 Size: 53 83 186 936 1499 944 2468 Fragments arranged by size: 2468 1499 944 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2715 3512 1937 Size: 778 797 4594 Fragments arranged by size: 4594 797 778 Acc65I G'GTAC_C Cuts at: 2847 3490 2847 Size: 643 5526 AclI AA'CG_TT Cuts at: 1563 4296 4669 1563 Size: 2733 373 3063 Fragments arranged by size: 3063 2733 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3007 3109 3175 3277 4232 5616 5884 1632 Size: 1375 102 66 102 955 1384 268 1917 Fragments arranged by size: 1917 1384 1375 955 268 102 102 66 AfeI AGC'GCT Cuts at: 3188 3272 3188 Size: 84 6085 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5972 AflIII A'CryG_T Cuts at: 1203 5068 6045 1203 Size: 3865 977 1327 Fragments arranged by size: 3865 1327 977 AgeI A'CCGG_T Cuts at: 5000 5000 Size: 6169 AhdI GACnn_n'nnGTC Cuts at: 4898 4898 Size: 6169 AleI CACnn'nnGTG Cuts at: 1166 3318 1166 Size: 2152 4017 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3392 1184 Size: 2208 3961 AlwNI CAG_nnn'CTG Cuts at: 2050 2071 2290 2317 3912 5484 2050 Size: 21 219 27 1595 1572 2735 Fragments arranged by size: 2735 1595 1572 219 27 21 ApaI G_GGCC'C Cuts at: 2214 2600 2214 Size: 386 5783 ApaLI G'TGCA_C Cuts at: 3143 4226 5382 3143 Size: 1083 1156 3930 Fragments arranged by size: 3930 1156 1083 ApoI r'AATT_y Cuts at: 1493 3478 3704 3856 5965 1493 Size: 1985 226 152 2109 1697 Fragments arranged by size: 2109 1985 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4723 160 Size: 872 3691 1606 Fragments arranged by size: 3691 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6169 AvaI C'yCGr_G Cuts at: 1870 1943 2214 2346 2471 3173 3494 1870 Size: 73 271 132 125 702 321 4545 Fragments arranged by size: 4545 702 321 271 132 125 73 BamHI G'GATC_C Cuts at: 2790 3499 3866 2790 Size: 709 367 5093 Fragments arranged by size: 5093 709 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2464 2686 2847 3368 3490 Size: 325 217 627 677 222 161 521 122 Cuts at: 3490 4945 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 677 627 521 325 222 217 161 122 BanII G_rGCy'C Cuts at: 1391 2133 2214 2441 2600 3203 3488 1391 Size: 742 81 227 159 603 285 4072 Fragments arranged by size: 4072 742 603 285 227 159 81 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2971 928 Size: 971 1072 4126 Fragments arranged by size: 4126 1072 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4360 4394 1903 Size: 34 2423 34 3678 Fragments arranged by size: 3678 2423 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4061 5271 4061 Size: 1210 4959 BclI T'GATC_A Cuts at: 1548 2935 1548 Size: 1387 4782 BfrBI ATG'CAT Cuts at: 2742 2742 Size: 6169 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4780 136 Size: 107 122 71 631 605 3108 1525 Fragments arranged by size: 3108 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6164 6164 Size: 6169 BlpI GC'TnA_GC Cuts at: 2511 2576 2754 3586 2511 Size: 65 178 832 5094 Fragments arranged by size: 5094 832 178 65 Bme1580I G_kGCm'C Cuts at: 1790 2214 2469 2600 2691 3147 3173 3371 4230 Size: 424 255 131 91 456 26 198 859 Cuts at: 4230 5386 1790 Size: 1156 2573 Fragments arranged by size: 2573 1156 859 456 424 255 198 131 91 26 BmgBI CAC'GTC Cuts at: 1318 2707 1318 Size: 1389 4780 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2906 3253 3900 Size: 416 53 83 399 24 1489 347 647 Cuts at: 3900 4858 442 Size: 958 1753 Fragments arranged by size: 1753 1489 958 647 416 399 347 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6169 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2149 2640 2149 Size: 491 5678 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2027 2312 2327 2536 2552 2610 2792 3187 Size: 788 285 15 209 16 58 182 395 Cuts at: 3187 3410 3462 4829 1239 Size: 223 52 1367 2579 Fragments arranged by size: 2579 1367 788 395 285 223 209 182 58 52 16 15 Bpu10I CC'TnA_GC Cuts at: 2225 2225 Size: 6169 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4294 5159 5457 4294 Size: 865 298 5006 Fragments arranged by size: 5006 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4832 882 Size: 617 3333 2219 Fragments arranged by size: 3333 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3466 3960 493 Size: 711 2262 494 2702 Fragments arranged by size: 2702 2262 711 494 BsaBI GATnn'nnATC Cuts at: 6163 6163 Size: 6169 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2651 3032 3976 4358 Size: 53 83 186 936 1118 381 944 382 Cuts at: 4358 4993 275 Size: 635 1451 Fragments arranged by size: 1451 1118 944 936 635 382 381 186 83 53 BsaWI w'CCGG_w Cuts at: 4602 5000 5274 5421 5832 4602 Size: 398 274 147 411 4939 Fragments arranged by size: 4939 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2636 2666 1178 Size: 30 165 30 1233 30 4681 Fragments arranged by size: 4681 1233 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2120 2141 2393 2744 1186 Size: 934 21 252 351 4611 Fragments arranged by size: 4611 934 351 252 21 BseYI C'CCAG_C Cuts at: 2153 2207 2566 2571 2749 5372 5868 2153 Size: 54 359 5 178 2623 496 2454 Fragments arranged by size: 2623 2454 496 359 178 54 5 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3238 3362 1163 Size: 573 1502 124 3970 Fragments arranged by size: 3970 1502 573 124 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2264 3115 3619 4380 5408 1540 Size: 275 186 263 851 504 761 1028 2301 Fragments arranged by size: 2301 1028 851 761 504 275 263 186 BsiHKAI G_wGCw'C Cuts at: 2859 3147 3203 4230 4315 5386 2859 Size: 288 56 1027 85 1071 3642 Fragments arranged by size: 3642 1071 1027 288 85 56 BsmI GAATG_Cn' Cuts at: 3680 3773 3680 Size: 93 6076 BsmBI CGTCTCn'nnnn_ Cuts at: 2135 2135 Size: 6169 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2133 2214 2441 2469 2600 2691 2859 Size: 399 343 81 227 28 131 91 168 Cuts at: 2859 3147 3173 3203 3371 3488 4230 4315 5386 Size: 288 26 30 168 117 742 85 1071 Cuts at: 5386 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 399 343 288 227 168 168 131 117 91 85 81 30 28 26 BspEI T'CCGG_A Cuts at: 5832 5832 Size: 6169 BspHI T'CATG_A Cuts at: 4058 5788 4058 Size: 1730 4439 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3008 3344 3384 3524 844 Size: 2164 336 40 140 3489 Fragments arranged by size: 3489 2164 336 140 40 BsrBI CCG'CTC Cuts at: 1394 1823 2373 2890 3073 3117 3241 4056 1394 Size: 429 550 517 183 44 124 815 3507 Fragments arranged by size: 3507 815 550 517 429 183 124 44 BsrDI GCAATG_nn' Cuts at: 65 1248 4664 4838 65 Size: 1183 3416 174 1396 Fragments arranged by size: 3416 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3430 4813 5000 1700 Size: 110 1620 1383 187 2869 Fragments arranged by size: 2869 1620 1383 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6169 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6169 BssSI C'ACGA_G Cuts at: 2393 4229 5241 2393 Size: 1836 1012 3321 Fragments arranged by size: 3321 1836 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 3261 794 Size: 2467 3702 BstBI TT'CG_AA Cuts at: 2007 4973 2007 Size: 2966 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6169 BstXI CCAn_nnnn'nTGG Cuts at: 2879 2879 Size: 6169 BstYI r'GATC_y Cuts at: 1972 2085 2286 2337 2521 2790 3499 3866 4251 Size: 113 201 51 184 269 709 367 385 Cuts at: 4251 4268 5720 5806 5818 6164 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 709 385 367 346 269 201 184 113 86 51 17 12 BstZ17I GTA'TAC Cuts at: 2716 2716 Size: 6169 BtgI C'CryG_G Cuts at: 513 1065 2022 2136 2385 2977 513 Size: 552 957 114 249 592 3705 Fragments arranged by size: 3705 957 592 552 249 114 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5430 Fragments arranged by size: 5430 683 56 BtsI GCAGTG_nn' Cuts at: 3685 4479 4499 3685 Size: 794 20 5355 Fragments arranged by size: 5355 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6169 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6134 DraI TTT'AAA Cuts at: 3473 3473 Size: 6169 DraIII CAC_nnn'GTG Cuts at: 3261 3261 Size: 6169 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5176 809 Size: 507 3860 1802 Fragments arranged by size: 3860 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2025 2261 2609 Size: 54 1425 50 275 18 195 236 348 Cuts at: 2609 2881 3349 3616 4505 6134 8 Size: 272 468 267 889 1629 43 Fragments arranged by size: 1629 1425 889 468 348 275 272 267 236 195 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2261 3616 1537 Size: 275 449 1355 4090 Fragments arranged by size: 4090 1355 449 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2076 2118 2183 2192 2532 4099 1353 Size: 723 42 65 9 340 1567 3423 Fragments arranged by size: 3423 1567 723 340 65 42 9 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2159 3350 4756 5130 5276 387 Size: 1064 138 570 1191 1406 374 146 1280 Fragments arranged by size: 1406 1280 1191 1064 570 374 146 138 EcoICRI GAG'CTC Cuts at: 3201 3201 Size: 6169 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2027 2312 2327 2536 2552 2610 2792 Size: 393 395 285 15 209 16 58 182 Cuts at: 2792 3007 3109 3175 3187 3277 3410 3462 4232 Size: 215 102 66 12 90 133 52 770 Cuts at: 4232 4829 5616 5884 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 787 770 597 395 393 285 268 215 209 182 133 102 90 66 58 52 16 15 12 EcoNI CCTnn'n_nnAGG Cuts at: 2075 2075 Size: 6169 EcoO109I rG'GnC_Cy Cuts at: 2068 2149 2211 2221 2596 2621 2783 2876 3446 Size: 81 62 10 375 25 162 93 570 Cuts at: 3446 2068 Size: 4791 Fragments arranged by size: 4791 570 375 162 93 81 62 25 10 EcoRI G'AATT_C Cuts at: 3478 3856 3478 Size: 378 5791 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2988 2988 Size: 6169 FspI TGC'GCA Cuts at: 2273 4675 2273 Size: 2402 3767 HaeII r_GCGC'y Cuts at: 1678 3190 3274 3300 5316 1678 Size: 1512 84 26 2016 2531 Fragments arranged by size: 2531 2016 1512 84 26 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2329 2361 2510 2542 2636 Size: 32 135 32 757 32 149 32 94 Cuts at: 2636 2668 2775 2807 4810 4842 4884 4916 1373 Size: 32 107 32 2003 32 42 32 2626 Fragments arranged by size: 2626 2003 757 149 135 107 94 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3513 3759 669 Size: 988 281 1575 246 3079 Fragments arranged by size: 3079 1575 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6169 HpaI GTT'AAC Cuts at: 3759 3759 Size: 6169 KpnI G_GTAC'C Cuts at: 2851 3494 2851 Size: 643 5526 MfeI C'AATT_G Cuts at: 3768 3768 Size: 6169 MluI A'CGCG_T Cuts at: 6045 6045 Size: 6169 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2778 3144 5283 5467 1305 Size: 1473 366 2139 184 2007 Fragments arranged by size: 2139 2007 1473 366 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2027 2611 2883 10 Size: 54 1425 343 195 584 272 3296 Fragments arranged by size: 3296 1425 584 343 272 195 54 MslI CAynn'nnrTG Cuts at: 518 1166 3318 4127 4486 4645 518 Size: 648 2152 809 359 159 2042 Fragments arranged by size: 2152 2042 809 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2253 2410 2434 2575 2602 2758 2770 3234 Size: 647 157 24 141 27 156 12 464 Cuts at: 3234 3391 3585 4262 5410 5655 1606 Size: 157 194 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 647 464 245 194 157 157 156 141 27 24 12 NcoI C'CATG_G Cuts at: 513 1065 2022 2977 513 Size: 552 957 955 3705 Fragments arranged by size: 3705 957 955 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6169 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6169 NotI GC'GGCC_GC Cuts at: 3616 3616 Size: 6169 NsiI A_TGCA'T Cuts at: 2744 2744 Size: 6169 NspI r_CATG'y Cuts at: 1132 1229 3527 5072 1132 Size: 97 2298 1545 2229 Fragments arranged by size: 2298 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6169 PciI A'CATG_T Cuts at: 5068 5068 Size: 6169 PflMI CCAn_nnn'nTGG Cuts at: 1108 2203 3433 1108 Size: 1095 1230 3844 Fragments arranged by size: 3844 1230 1095 PfoI T'CCnGG_A Cuts at: 2289 3439 2289 Size: 1150 5019 PmeI GTTT'AAAC Cuts at: 3473 3473 Size: 6169 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4238 4270 5751 5783 4238 Size: 32 1481 32 4624 Fragments arranged by size: 4624 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2068 2149 2783 2068 Size: 81 634 5454 Fragments arranged by size: 5454 634 81 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6169 PsiI TTA'TAA Cuts at: 3739 3739 Size: 6169 PspOMI G'GGCC_C Cuts at: 2210 2596 2210 Size: 386 5783 PstI C_TGCA'G Cuts at: 830 2409 3521 830 Size: 1579 1112 3478 Fragments arranged by size: 3478 1579 1112 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6169 PvuII CAG'CTG Cuts at: 2575 2575 Size: 6169 SacI G_AGCT'C Cuts at: 3203 3203 Size: 6169 SalI G'TCGA_C Cuts at: 1936 3511 1936 Size: 1575 4594 SapI GCTCTTCn'nnn_ Cuts at: 2183 2192 2183 Size: 9 6160 SbfI CC_TGCA'GG Cuts at: 3521 3521 Size: 6169 ScaI AGT'ACT Cuts at: 4417 6129 4417 Size: 1712 4457 SfcI C'TryA_G Cuts at: 826 1045 2405 3517 4652 5333 5524 826 Size: 219 1360 1112 1135 681 191 1471 Fragments arranged by size: 1471 1360 1135 1112 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6169 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6169 SmaI CCC'GGG Cuts at: 1872 2216 3496 1872 Size: 344 1280 4545 Fragments arranged by size: 4545 1280 344 SmlI C'TyrA_G Cuts at: 820 1017 1943 4273 5174 5436 820 Size: 197 926 2330 901 262 1553 Fragments arranged by size: 2330 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3466 3960 493 Size: 2973 494 2702 Fragments arranged by size: 2973 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 6169 SphI G_CATG'C Cuts at: 1132 3527 1132 Size: 2395 3774 SspI AAT'ATT Cuts at: 5 52 4093 5 Size: 47 4041 2081 Fragments arranged by size: 4041 2081 47 StuI AGG'CCT Cuts at: 1517 2932 1517 Size: 1415 4754 StyI C'CwwG_G Cuts at: 513 1065 2022 2058 2277 2977 3608 513 Size: 552 957 36 219 700 631 3074 Fragments arranged by size: 3074 957 700 631 552 219 36 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3272 4196 4213 4366 4551 4996 325 Size: 1255 1692 924 17 153 185 445 1498 Fragments arranged by size: 1692 1498 1255 924 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4415 6127 96 Size: 274 80 33 51 1443 2438 1712 138 Fragments arranged by size: 2438 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6129 6161 6129 Size: 32 6137 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6169 XbaI T'CTAG_A Cuts at: 3505 3505 Size: 6169 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2204 2204 Size: 6169 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6169 XmaI C'CCGG_G Cuts at: 1870 2214 3494 1870 Size: 344 1280 4545 Fragments arranged by size: 4545 1280 344 XmnI GAAnn'nnTTC Cuts at: 3188 3272 4298 3188 Size: 84 1026 5059 Fragments arranged by size: 5059 1026 84 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3033 3977 276 Size: 53 83 186 936 1499 944 2468 Fragments arranged by size: 2468 1499 944 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BaeI BbeI BbvCI BsiWI Bsu36I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PspXI PsrI RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI