(Circular) (Six-base) MAPSORT of: pFN21ASDA0547.seq Check: 9617 from: 1 to: 6787 pFN21ASDA0547 6787 bp With 182 enzymes: SgfI * January 16, 2009 15:30 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4597 278 Size: 53 83 186 936 3061 2468 Fragments arranged by size: 3061 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3753 4130 1937 Size: 1816 377 4594 Fragments arranged by size: 4594 1816 377 Acc65I G'GTAC_C Cuts at: 4022 4108 4022 Size: 86 6701 AclI AA'CG_TT Cuts at: 1563 4914 5287 1563 Size: 3351 373 3063 Fragments arranged by size: 3351 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2945 3018 3107 3636 3990 4850 6234 6502 Size: 1313 73 89 529 354 860 1384 268 Cuts at: 6502 1632 Size: 1917 Fragments arranged by size: 1917 1384 1313 860 529 354 268 89 73 AflII C'TTAA_G Cuts at: 820 1017 4066 820 Size: 197 3049 3541 Fragments arranged by size: 3541 3049 197 AflIII A'CryG_T Cuts at: 1203 5686 6663 1203 Size: 4483 977 1327 Fragments arranged by size: 4483 1327 977 AgeI A'CCGG_T Cuts at: 5618 5618 Size: 6787 AhdI GACnn_n'nnGTC Cuts at: 5516 5516 Size: 6787 AleI CACnn'nnGTG Cuts at: 1166 2984 1166 Size: 1818 4969 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2503 1184 Size: 1319 5468 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3998 4030 3998 Size: 32 6755 AlwNI CAG_nnn'CTG Cuts at: 3639 3985 4530 6102 3639 Size: 346 545 1572 4324 Fragments arranged by size: 4324 1572 545 346 ApaI G_GGCC'C Cuts at: 2030 2030 Size: 6787 ApaLI G'TGCA_C Cuts at: 4844 6000 4844 Size: 1156 5631 ApoI r'AATT_y Cuts at: 1493 2840 2906 4096 4322 4474 6583 1493 Size: 1347 66 1190 226 152 2109 1697 Fragments arranged by size: 2109 1697 1347 1190 226 152 66 AscI GG'CGCG_CC Cuts at: 2159 2247 2159 Size: 88 6699 AseI AT'TA_AT Cuts at: 160 1032 3935 5341 160 Size: 872 2903 1406 1606 Fragments arranged by size: 2903 1606 1406 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6787 AvaI C'yCGr_G Cuts at: 1870 1943 2340 2603 3677 4112 1870 Size: 73 397 263 1074 435 4545 Fragments arranged by size: 4545 1074 435 397 263 73 BamHI G'GATC_C Cuts at: 3591 4117 4484 3591 Size: 526 367 5894 Fragments arranged by size: 5894 526 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2540 2787 2822 4022 4108 Size: 325 217 627 753 247 35 1200 86 Cuts at: 4108 5563 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 1200 753 627 325 247 217 86 35 BanII G_rGCy'C Cuts at: 1391 2030 2454 2553 3122 3661 3896 4106 1391 Size: 639 424 99 569 539 235 210 4072 Fragments arranged by size: 4072 639 569 539 424 235 210 99 BbeI G_GCGC'C Cuts at: 2544 2791 2544 Size: 247 6540 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3103 928 Size: 971 1204 4612 Fragments arranged by size: 4612 1204 971 BbvCI CC'TCA_GC Cuts at: 2087 2087 Size: 6787 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2708 2742 4978 5012 1903 Size: 34 771 34 2236 34 3678 Fragments arranged by size: 3678 2236 771 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3968 4679 5889 3968 Size: 711 1210 4866 Fragments arranged by size: 4866 1210 711 BclI T'GATC_A Cuts at: 1548 3870 1548 Size: 2322 4465 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3237 5398 136 Size: 107 122 71 631 605 1565 2161 1525 Fragments arranged by size: 2161 1565 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2274 6782 2274 Size: 4508 2279 BlpI GC'TnA_GC Cuts at: 4204 4204 Size: 6787 Bme1580I G_kGCm'C Cuts at: 1790 2030 4848 6004 1790 Size: 240 2818 1156 2573 Fragments arranged by size: 2818 2573 1156 240 BmgBI CAC'GTC Cuts at: 1318 2374 2773 1318 Size: 1056 399 5332 Fragments arranged by size: 5332 1056 399 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3332 3590 4518 Size: 416 53 83 399 24 1915 258 928 Cuts at: 4518 5476 442 Size: 958 1753 Fragments arranged by size: 1915 1753 958 928 416 399 258 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6787 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2987 2987 Size: 6787 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2501 2777 3273 3347 3690 3879 5447 1239 Size: 1262 276 496 74 343 189 1568 2579 Fragments arranged by size: 2579 1568 1262 496 343 276 189 74 Bpu10I CC'TnA_GC Cuts at: 2087 2087 Size: 6787 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2676 3136 3184 4912 5777 6075 2676 Size: 460 48 1728 865 298 3388 Fragments arranged by size: 3388 1728 865 460 298 48 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3680 3712 5450 882 Size: 617 2181 32 1738 2219 Fragments arranged by size: 2219 2181 1738 617 32 BsaAI yAC'GTr Cuts at: 493 1204 2119 4084 4578 493 Size: 711 915 1965 494 2702 Fragments arranged by size: 2702 1965 915 711 494 BsaBI GATnn'nnATC Cuts at: 6781 6781 Size: 6787 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2408 2541 2697 2788 Size: 53 83 186 936 875 133 156 91 Cuts at: 2788 3271 4594 4976 5611 275 Size: 483 1323 382 635 1451 Fragments arranged by size: 1451 1323 936 875 635 483 382 186 156 133 91 83 53 BsaWI w'CCGG_w Cuts at: 2368 5220 5618 5892 6039 6450 2368 Size: 2852 398 274 147 411 2705 Fragments arranged by size: 2852 2705 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2441 2471 2957 2987 3852 Size: 30 165 30 1038 30 486 30 865 Cuts at: 3852 3882 1178 Size: 30 4083 Fragments arranged by size: 4083 1038 865 486 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2543 2927 3704 3838 1186 Size: 1357 384 777 134 4135 Fragments arranged by size: 4135 1357 777 384 134 BseYI C'CCAG_C Cuts at: 2643 4073 5990 6486 2643 Size: 1430 1917 496 2944 Fragments arranged by size: 2944 1917 1430 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2141 3159 3625 1163 Size: 573 405 1018 466 4325 Fragments arranged by size: 4325 1018 573 466 405 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4237 4998 6026 1540 Size: 275 186 2236 761 1028 2301 Fragments arranged by size: 2301 2236 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3711 3804 3896 4848 4933 6004 3711 Size: 93 92 952 85 1071 4494 Fragments arranged by size: 4494 1071 952 93 92 85 BsmI GAATG_Cn' Cuts at: 3466 3625 4298 4391 3466 Size: 159 673 93 5862 Fragments arranged by size: 5862 673 159 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2399 2399 Size: 6787 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2030 2454 2553 3122 3661 3711 3804 Size: 399 240 424 99 569 539 50 93 Cuts at: 3804 3896 4106 4848 4933 6004 1391 Size: 92 210 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 569 539 424 399 240 210 99 93 92 85 50 BspEI T'CCGG_A Cuts at: 2368 6450 2368 Size: 4082 2705 BspHI T'CATG_A Cuts at: 4676 6406 4676 Size: 1730 5057 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4142 844 Size: 3298 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2173 4674 1394 Size: 429 350 2501 3507 Fragments arranged by size: 3507 2501 429 350 BsrDI GCAATG_nn' Cuts at: 65 1248 5282 5456 65 Size: 1183 4034 174 1396 Fragments arranged by size: 4034 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2874 5431 5618 1700 Size: 110 1064 2557 187 2869 Fragments arranged by size: 2869 2557 1064 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6787 BssHII G'CGCG_C Cuts at: 1925 2108 2159 2224 2247 2268 1925 Size: 183 51 65 23 21 6444 Fragments arranged by size: 6444 183 65 51 23 21 BssSI C'ACGA_G Cuts at: 4847 5859 4847 Size: 1012 5775 BstAPI GCAn_nnn'nTGC Cuts at: 794 2221 794 Size: 1427 5360 BstBI TT'CG_AA Cuts at: 2007 5591 2007 Size: 3584 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6787 BstYI r'GATC_y Cuts at: 1972 2274 3591 3831 4117 4484 4869 4886 6338 Size: 302 1317 240 286 367 385 17 1452 Cuts at: 6338 6424 6436 6782 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 1317 385 367 346 302 286 240 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2022 2325 2544 2619 2826 3926 513 Size: 552 957 303 219 75 207 1100 3374 Fragments arranged by size: 3374 1100 957 552 303 219 207 75 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2614 487 Size: 683 56 1388 4660 Fragments arranged by size: 4660 1388 683 56 BtsI GCAGTG_nn' Cuts at: 2215 2848 3169 4303 5097 5117 2215 Size: 633 321 1134 794 20 3885 Fragments arranged by size: 3885 1134 794 633 321 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6787 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6752 DraI TTT'AAA Cuts at: 4091 4091 Size: 6787 DraIII CAC_nnn'GTG Cuts at: 2221 3453 2221 Size: 1232 5555 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5794 809 Size: 507 4478 1802 Fragments arranged by size: 4478 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2103 2580 2704 Size: 54 1425 50 275 18 273 477 124 Cuts at: 2704 3097 3430 3929 4234 5123 6752 8 Size: 393 333 499 305 889 1629 43 Fragments arranged by size: 1629 1425 889 499 477 393 333 305 275 273 124 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4234 1537 Size: 275 2422 4090 Fragments arranged by size: 4090 2422 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3083 3966 4717 1353 Size: 1730 883 751 3423 Fragments arranged by size: 3423 1730 883 751 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5374 5748 5894 387 Size: 1064 138 3785 374 146 1280 Fragments arranged by size: 3785 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3894 3894 Size: 6787 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2501 2777 2945 3018 3107 3273 3347 Size: 393 869 276 168 73 89 166 74 Cuts at: 3347 3636 3690 3879 3990 4850 5447 6234 6502 Size: 289 54 189 111 860 597 787 268 Cuts at: 6502 1239 Size: 1524 Fragments arranged by size: 1524 869 860 787 597 393 289 276 268 189 168 166 111 89 74 73 54 EcoNI CCTnn'n_nnAGG Cuts at: 2582 2582 Size: 6787 EcoO109I rG'GnC_Cy Cuts at: 2027 2125 2533 3076 3403 2027 Size: 98 408 543 327 5411 Fragments arranged by size: 5411 543 408 327 98 EcoRI G'AATT_C Cuts at: 2840 4096 4474 2840 Size: 1256 378 5153 Fragments arranged by size: 5153 1256 378 FspI TGC'GCA Cuts at: 5293 5293 Size: 6787 HaeII r_GCGC'y Cuts at: 1678 2085 2292 2544 2791 5934 1678 Size: 407 207 252 247 3143 2531 Fragments arranged by size: 3143 2531 407 252 247 207 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2266 2298 2441 2473 2546 Size: 32 135 32 694 32 143 32 73 Cuts at: 2546 2578 2674 2706 2957 2989 3197 3229 5428 Size: 32 96 32 251 32 208 32 2199 Cuts at: 5428 5460 5502 5534 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2199 694 251 208 143 135 96 73 42 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2427 4131 4377 669 Size: 988 281 489 1704 246 3079 Fragments arranged by size: 3079 1704 988 489 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6787 HpaI GTT'AAC Cuts at: 2427 4377 2427 Size: 1950 4837 KasI G'GCGC_C Cuts at: 2540 2787 2540 Size: 247 6540 KpnI G_GTAC'C Cuts at: 4026 4112 4026 Size: 86 6701 MfeI C'AATT_G Cuts at: 4386 4386 Size: 6787 MluI A'CGCG_T Cuts at: 6663 6663 Size: 6787 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3805 5901 6085 1305 Size: 2500 2096 184 2007 Fragments arranged by size: 2500 2096 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2706 3099 3931 10 Size: 54 1425 343 874 393 832 2866 Fragments arranged by size: 2866 1425 874 832 393 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2984 4745 5104 5263 518 Size: 648 1818 1761 359 159 2042 Fragments arranged by size: 2042 1818 1761 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2327 2546 2621 2785 4203 4880 6028 6273 Size: 721 219 75 164 1418 677 1148 245 Cuts at: 6273 1606 Size: 2120 Fragments arranged by size: 2120 1418 1148 721 677 245 219 164 75 NaeI GCC'GGC Cuts at: 2876 2876 Size: 6787 NarI GG'CG_CC Cuts at: 2541 2788 2541 Size: 247 6540 NcoI C'CATG_G Cuts at: 513 1065 2022 3926 513 Size: 552 957 1904 3374 Fragments arranged by size: 3374 1904 957 552 NdeI CA'TA_TG Cuts at: 387 3919 387 Size: 3532 3255 NgoMIV G'CCGG_C Cuts at: 2874 2874 Size: 6787 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6787 NotI GC'GGCC_GC Cuts at: 4234 4234 Size: 6787 NspI r_CATG'y Cuts at: 1132 1229 4145 5690 1132 Size: 97 2916 1545 2229 Fragments arranged by size: 2916 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 2536 1348 Size: 1188 5599 PciI A'CATG_T Cuts at: 5686 5686 Size: 6787 PflMI CCAn_nnn'nTGG Cuts at: 1108 2524 1108 Size: 1416 5371 PfoI T'CCnGG_A Cuts at: 3856 3856 Size: 6787 PmeI GTTT'AAAC Cuts at: 4091 4091 Size: 6787 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4856 4888 6369 6401 4856 Size: 32 1481 32 5242 Fragments arranged by size: 5242 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2125 3076 3403 2125 Size: 951 327 5509 Fragments arranged by size: 5509 951 327 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6787 PsiI TTA'TAA Cuts at: 4357 4357 Size: 6787 PspOMI G'GGCC_C Cuts at: 2026 2026 Size: 6787 PspXI vC'TCGA_Gb Cuts at: 2603 2603 Size: 6787 PstI C_TGCA'G Cuts at: 830 2350 4139 830 Size: 1520 1789 3478 Fragments arranged by size: 3478 1789 1520 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6787 SacI G_AGCT'C Cuts at: 3896 3896 Size: 6787 SacII CC_GC'GG Cuts at: 2328 2547 2622 2328 Size: 219 75 6493 Fragments arranged by size: 6493 219 75 SalI G'TCGA_C Cuts at: 1936 4129 1936 Size: 2193 4594 SapI GCTCTTCn'nnn_ Cuts at: 3966 3966 Size: 6787 SbfI CC_TGCA'GG Cuts at: 4139 4139 Size: 6787 ScaI AGT'ACT Cuts at: 5035 6747 5035 Size: 1712 5075 SfcI C'TryA_G Cuts at: 826 1045 2346 3882 4135 5270 5951 6142 826 Size: 219 1301 1536 253 1135 681 191 1471 Fragments arranged by size: 1536 1471 1301 1135 681 253 219 191 SfoI GGC'GCC Cuts at: 2542 2789 2542 Size: 247 6540 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6787 SgrAI Cr'CCGG_yG Cuts at: 1700 2874 1700 Size: 1174 5613 SmaI CCC'GGG Cuts at: 1872 2342 4114 1872 Size: 470 1772 4545 Fragments arranged by size: 4545 1772 470 SmlI C'TyrA_G Cuts at: 820 1017 1943 2603 2691 3115 3199 4066 4891 Size: 197 926 660 88 424 84 867 825 Cuts at: 4891 5792 6054 820 Size: 901 262 1553 Fragments arranged by size: 1553 926 901 867 825 660 424 262 197 88 84 SnaBI TAC'GTA Cuts at: 493 4084 4578 493 Size: 3591 494 2702 Fragments arranged by size: 3591 2702 494 SpeI A'CTAG_T Cuts at: 152 3953 152 Size: 3801 2986 SphI G_CATG'C Cuts at: 1132 4145 1132 Size: 3013 3774 SrfI GCCC'GGGC Cuts at: 2342 2342 Size: 6787 SspI AAT'ATT Cuts at: 5 52 2890 3503 4711 5 Size: 47 2838 613 1208 2081 Fragments arranged by size: 2838 2081 1208 613 47 StuI AGG'CCT Cuts at: 1517 2689 1517 Size: 1172 5615 StyI C'CwwG_G Cuts at: 513 1065 2022 3351 3776 3926 3991 4226 513 Size: 552 957 1329 425 150 65 235 3074 Fragments arranged by size: 3074 1329 957 552 425 235 150 65 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2581 4814 4831 4984 5169 5614 325 Size: 1255 1001 2233 17 153 185 445 1498 Fragments arranged by size: 2233 1498 1255 1001 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3541 5033 6745 Size: 274 80 33 51 1443 1564 1492 1712 Cuts at: 6745 96 Size: 138 Fragments arranged by size: 1712 1564 1492 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3852 3884 6747 6779 3852 Size: 32 2863 32 3860 Fragments arranged by size: 3860 2863 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6787 XbaI T'CTAG_A Cuts at: 4123 4123 Size: 6787 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2575 2575 Size: 6787 XhoI C'TCGA_G Cuts at: 1943 2603 1943 Size: 660 6127 XmaI C'CCGG_G Cuts at: 1870 2340 4112 1870 Size: 470 1772 4545 Fragments arranged by size: 4545 1772 470 XmnI GAAnn'nnTTC Cuts at: 4916 4916 Size: 6787 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4595 276 Size: 53 83 186 936 3061 2468 Fragments arranged by size: 3061 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AscI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI SacI SacII SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AvrII BaeI BfrBI BsiWI BstXI BstZ17I Bsu36I EcoRV FalI FseI FspAI NruI NsiI PacI PmlI PsrI PvuII RsrII SanDI SexAI SfiI SwaI