(Circular) (Six-base) MAPSORT of: pFN21ASDA0544.seq Check: 5555 from: 1 to: 6385 pFN21ASDA0544 6385 bp With 182 enzymes: SgfI * January 16, 2009 15:30 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3628 3628 Size: 6385 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4195 278 Size: 53 83 186 936 2659 2468 Fragments arranged by size: 2659 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2466 3728 1937 Size: 529 1262 4594 Fragments arranged by size: 4594 1262 529 Acc65I G'GTAC_C Cuts at: 3706 3706 Size: 6385 AclI AA'CG_TT Cuts at: 1563 4512 4885 1563 Size: 2949 373 3063 Fragments arranged by size: 3063 2949 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2190 3028 4448 5832 6100 1632 Size: 558 838 1420 1384 268 1917 Fragments arranged by size: 1917 1420 1384 838 558 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6188 AflIII A'CryG_T Cuts at: 1203 5284 6261 1203 Size: 4081 977 1327 Fragments arranged by size: 4081 1327 977 AgeI A'CCGG_T Cuts at: 5216 5216 Size: 6385 AhdI GACnn_n'nnGTC Cuts at: 3289 5114 3289 Size: 1825 4560 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6385 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6385 AlwNI CAG_nnn'CTG Cuts at: 3067 4128 5700 3067 Size: 1061 1572 3752 Fragments arranged by size: 3752 1572 1061 ApaI G_GGCC'C Cuts at: 2989 3651 2989 Size: 662 5723 ApaLI G'TGCA_C Cuts at: 4442 5598 4442 Size: 1156 5229 ApoI r'AATT_y Cuts at: 1493 2150 3694 3920 4072 6181 1493 Size: 657 1544 226 152 2109 1697 Fragments arranged by size: 2109 1697 1544 657 226 152 AseI AT'TA_AT Cuts at: 160 1032 4939 160 Size: 872 3907 1606 Fragments arranged by size: 3907 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6385 AvaI C'yCGr_G Cuts at: 1870 1943 2364 3197 3555 3596 3710 1870 Size: 73 421 833 358 41 114 4545 Fragments arranged by size: 4545 833 421 358 114 73 41 BamHI G'GATC_C Cuts at: 3715 4082 3715 Size: 367 6018 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3706 5161 618 Size: 325 217 627 1919 1455 1842 Fragments arranged by size: 1919 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2030 2262 2483 2989 3028 3186 3651 3704 Size: 639 232 221 506 39 158 465 53 Cuts at: 3704 1391 Size: 4072 Fragments arranged by size: 4072 639 506 465 232 221 158 53 39 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2273 3390 928 Size: 971 374 1117 3923 Fragments arranged by size: 3923 1117 971 374 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2384 2418 4576 4610 1903 Size: 34 447 34 2158 34 3678 Fragments arranged by size: 3678 2158 447 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4277 5487 4277 Size: 1210 5175 BclI T'GATC_A Cuts at: 1548 1548 Size: 6385 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2411 3125 3351 Size: 107 122 71 631 605 739 714 226 Cuts at: 3351 4996 136 Size: 1645 1525 Fragments arranged by size: 1645 1525 739 714 631 605 226 122 107 71 BglII A'GATC_T Cuts at: 2778 6380 2778 Size: 3602 2783 BlpI GC'TnA_GC Cuts at: 3802 3802 Size: 6385 Bme1580I G_kGCm'C Cuts at: 1790 2989 3651 4446 5602 1790 Size: 1199 662 795 1156 2573 Fragments arranged by size: 2573 1199 1156 795 662 BmgBI CAC'GTC Cuts at: 1318 3442 1318 Size: 2124 4261 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3470 4116 5074 Size: 416 53 83 399 24 2053 646 958 Cuts at: 5074 442 Size: 1753 Fragments arranged by size: 2053 1753 958 646 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6385 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3205 3448 3205 Size: 243 6142 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2402 3089 5045 1239 Size: 1163 687 1956 2579 Fragments arranged by size: 2579 1956 1163 687 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4510 5375 5673 4510 Size: 865 298 5222 Fragments arranged by size: 5222 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2492 2804 5048 882 Size: 617 993 312 2244 2219 Fragments arranged by size: 2244 2219 993 617 312 BsaAI yAC'GTr Cuts at: 493 1204 2677 3682 4176 493 Size: 711 1473 1005 494 2702 Fragments arranged by size: 2702 1473 1005 711 494 BsaBI GATnn'nnATC Cuts at: 6379 6379 Size: 6385 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2397 3447 4192 4574 Size: 53 83 186 936 864 1050 745 382 Cuts at: 4574 5209 275 Size: 635 1451 Fragments arranged by size: 1451 1050 936 864 745 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2108 4818 5216 5490 5637 6048 2108 Size: 2710 398 274 147 411 2445 Fragments arranged by size: 2710 2445 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2637 2667 3104 3134 1178 Size: 30 165 30 1234 30 437 30 4429 Fragments arranged by size: 4429 1234 437 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2157 2456 3437 1186 Size: 971 299 981 4134 Fragments arranged by size: 4134 981 971 299 BseYI C'CCAG_C Cuts at: 5588 6084 5588 Size: 496 5889 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5812 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3835 4596 5624 1540 Size: 275 186 1834 761 1028 2301 Fragments arranged by size: 2301 1834 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2373 2682 3084 3603 4446 4531 5602 2373 Size: 309 402 519 843 85 1071 3156 Fragments arranged by size: 3156 1071 843 519 402 309 85 BsmI GAATG_Cn' Cuts at: 3896 3989 3896 Size: 93 6292 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2030 2262 2373 2483 2682 2989 3028 Size: 399 240 232 111 110 199 307 39 Cuts at: 3028 3084 3186 3603 3651 3704 4446 4531 5602 Size: 56 102 417 48 53 742 85 1071 Cuts at: 5602 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 417 399 307 240 232 199 111 110 102 85 56 53 48 39 BspEI T'CCGG_A Cuts at: 2108 6048 2108 Size: 3940 2445 BspHI T'CATG_A Cuts at: 4274 6004 4274 Size: 1730 4655 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2883 3628 3642 3740 844 Size: 2039 745 14 98 3489 Fragments arranged by size: 3489 2039 745 98 14 BsrBI CCG'CTC Cuts at: 1394 1823 4272 1394 Size: 429 2449 3507 Fragments arranged by size: 3507 2449 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4880 5054 65 Size: 1183 3632 174 1396 Fragments arranged by size: 3632 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2970 5029 5216 1700 Size: 110 1160 2059 187 2869 Fragments arranged by size: 2869 2059 1160 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6385 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6385 BssSI C'ACGA_G Cuts at: 2255 4445 5457 2255 Size: 2190 1012 3183 Fragments arranged by size: 3183 2190 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6385 BstBI TT'CG_AA Cuts at: 2007 5189 2007 Size: 3182 3203 BstEII G'GTnAC_C Cuts at: 1184 2174 1184 Size: 990 5395 BstYI r'GATC_y Cuts at: 1972 2197 2778 2997 3715 4082 4467 4484 5936 Size: 225 581 219 718 367 385 17 1452 Cuts at: 5936 6022 6034 6380 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 718 581 385 367 346 225 219 86 17 12 BstZ17I GTA'TAC Cuts at: 2467 2467 Size: 6385 BtgI C'CryG_G Cuts at: 513 1065 2022 513 Size: 552 957 4876 Fragments arranged by size: 4876 957 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2032 487 Size: 683 56 806 4840 Fragments arranged by size: 4840 806 683 56 BtsI GCAGTG_nn' Cuts at: 3901 4695 4715 3901 Size: 794 20 5571 Fragments arranged by size: 5571 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6385 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6350 DraI TTT'AAA Cuts at: 2733 3097 3689 2733 Size: 364 592 5429 Fragments arranged by size: 5429 592 364 DraIII CAC_nnn'GTG Cuts at: 3464 3464 Size: 6385 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5392 809 Size: 507 4076 1802 Fragments arranged by size: 4076 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2412 2665 3126 Size: 54 1425 50 275 18 582 253 461 Cuts at: 3126 3231 3607 3832 4721 6350 8 Size: 105 376 225 889 1629 43 Fragments arranged by size: 1629 1425 889 582 461 376 275 253 225 105 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3832 1537 Size: 275 2020 4090 Fragments arranged by size: 4090 2020 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3408 3513 4315 1353 Size: 2055 105 802 3423 Fragments arranged by size: 3423 2055 802 105 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4972 5346 5492 387 Size: 1064 138 3383 374 146 1280 Fragments arranged by size: 3383 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2190 2402 3028 3089 4448 5045 5832 Size: 393 558 212 626 61 1359 597 787 Cuts at: 5832 6100 1239 Size: 268 1524 Fragments arranged by size: 1524 1359 787 626 597 558 393 268 212 61 EcoNI CCTnn'n_nnAGG Cuts at: 2771 3293 2771 Size: 522 5863 EcoO109I rG'GnC_Cy Cuts at: 2986 3370 3552 3647 2986 Size: 384 182 95 5724 Fragments arranged by size: 5724 384 182 95 EcoRI G'AATT_C Cuts at: 3694 4072 3694 Size: 378 6007 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2575 2575 Size: 6385 FspI TGC'GCA Cuts at: 4891 4891 Size: 6385 HaeII r_GCGC'y Cuts at: 1678 3206 5532 1678 Size: 1528 2326 2531 Fragments arranged by size: 2531 2326 1528 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3068 3100 3275 3307 3518 Size: 32 135 32 1496 32 175 32 211 Cuts at: 3518 3521 3550 3553 3644 3676 5026 5058 5100 Size: 3 29 3 91 32 1350 32 42 Cuts at: 5100 5132 1373 Size: 32 2626 Fragments arranged by size: 2626 1496 1350 211 175 135 91 42 32 32 32 32 32 32 32 29 3 3 HincII GTy'rAC Cuts at: 669 1657 1938 3729 3975 669 Size: 988 281 1791 246 3079 Fragments arranged by size: 3079 1791 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6385 HpaI GTT'AAC Cuts at: 3975 3975 Size: 6385 KpnI G_GTAC'C Cuts at: 3710 3710 Size: 6385 MfeI C'AATT_G Cuts at: 3984 3984 Size: 6385 MluI A'CGCG_T Cuts at: 6261 6261 Size: 6385 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2895 5499 5683 1305 Size: 1590 2604 184 2007 Fragments arranged by size: 2604 2007 1590 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2414 2667 3128 10 Size: 54 1425 343 582 253 461 3267 Fragments arranged by size: 3267 1425 582 461 343 253 54 MslI CAynn'nnrTG Cuts at: 518 1166 4343 4702 4861 518 Size: 648 3177 359 159 2042 Fragments arranged by size: 3177 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3303 3801 4478 5626 5871 1606 Size: 1697 498 677 1148 245 2120 Fragments arranged by size: 2120 1697 1148 677 498 245 NaeI GCC'GGC Cuts at: 2972 2972 Size: 6385 NcoI C'CATG_G Cuts at: 513 1065 2022 513 Size: 552 957 4876 Fragments arranged by size: 4876 957 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6385 NgoMIV G'CCGG_C Cuts at: 2970 2970 Size: 6385 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6385 NotI GC'GGCC_GC Cuts at: 3832 3832 Size: 6385 NspI r_CATG'y Cuts at: 1132 1229 3743 5288 1132 Size: 97 2514 1545 2229 Fragments arranged by size: 2514 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 3643 1348 Size: 2295 4090 PciI A'CATG_T Cuts at: 5284 5284 Size: 6385 PflMI CCAn_nnn'nTGG Cuts at: 1108 2632 3067 3331 3464 1108 Size: 1524 435 264 133 4029 Fragments arranged by size: 4029 1524 435 264 133 PfoI T'CCnGG_A Cuts at: 3330 3330 Size: 6385 PmeI GTTT'AAAC Cuts at: 3689 3689 Size: 6385 PmlI CAC'GTG Cuts at: 2677 2677 Size: 6385 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4454 4486 5967 5999 4454 Size: 32 1481 32 4840 Fragments arranged by size: 4840 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3552 3552 Size: 6385 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6385 PsiI TTA'TAA Cuts at: 3955 3955 Size: 6385 PspOMI G'GGCC_C Cuts at: 2985 3647 2985 Size: 662 5723 PspXI vC'TCGA_Gb Cuts at: 3197 3197 Size: 6385 PstI C_TGCA'G Cuts at: 830 2775 2997 3585 3737 830 Size: 1945 222 588 152 3478 Fragments arranged by size: 3478 1945 588 222 152 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6385 PvuII CAG'CTG Cuts at: 3303 3303 Size: 6385 SalI G'TCGA_C Cuts at: 1936 3727 1936 Size: 1791 4594 SanDI GG'GwC_CC Cuts at: 3552 3552 Size: 6385 SbfI CC_TGCA'GG Cuts at: 2775 3585 3737 2775 Size: 810 152 5423 Fragments arranged by size: 5423 810 152 ScaI AGT'ACT Cuts at: 4633 6345 4633 Size: 1712 4673 SfcI C'TryA_G Cuts at: 826 1045 2374 2489 2771 2993 3581 3733 4868 Size: 219 1329 115 282 222 588 152 1135 Cuts at: 4868 5549 5740 826 Size: 681 191 1471 Fragments arranged by size: 1471 1329 1135 681 588 282 222 219 191 152 115 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6385 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6385 SmaI CCC'GGG Cuts at: 1872 2366 3712 1872 Size: 494 1346 4545 Fragments arranged by size: 4545 1346 494 SmlI C'TyrA_G Cuts at: 820 1017 1943 3197 4489 5390 5652 820 Size: 197 926 1254 1292 901 262 1553 Fragments arranged by size: 1553 1292 1254 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3682 4176 493 Size: 3189 494 2702 Fragments arranged by size: 3189 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 6385 SphI G_CATG'C Cuts at: 1132 3743 1132 Size: 2611 3774 SspI AAT'ATT Cuts at: 5 52 4309 5 Size: 47 4257 2081 Fragments arranged by size: 4257 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6385 StyI C'CwwG_G Cuts at: 513 1065 2022 3824 513 Size: 552 957 1802 3074 Fragments arranged by size: 3074 1802 957 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3452 4412 4429 4582 4767 5212 325 Size: 1255 1872 960 17 153 185 445 1498 Fragments arranged by size: 1872 1498 1255 960 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4631 6343 96 Size: 274 80 33 51 1443 2654 1712 138 Fragments arranged by size: 2654 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2637 2669 3104 3136 6345 6377 2637 Size: 32 435 32 3209 32 2645 Fragments arranged by size: 3209 2645 435 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2756 3164 1316 Size: 1440 408 4537 Fragments arranged by size: 4537 1440 408 XbaI T'CTAG_A Cuts at: 3721 3721 Size: 6385 XhoI C'TCGA_G Cuts at: 1943 3197 1943 Size: 1254 5131 XmaI C'CCGG_G Cuts at: 1870 2364 3710 1870 Size: 494 1346 4545 Fragments arranged by size: 4545 1346 494 XmnI GAAnn'nnTTC Cuts at: 4514 4514 Size: 6385 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4193 276 Size: 53 83 186 936 2659 2468 Fragments arranged by size: 2659 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstXI Bsu36I EcoICRI EcoRV FseI FspAI KasI NarI NruI NsiI PacI PsrI RsrII SacI SacII SapI SexAI SfiI SfoI SrfI SwaI XcmI