(Circular) (Six-base) MAPSORT of: pFN21ASDA0529.seq Check: 967 from: 1 to: 7585 pFN21ASDA0529 7585 bp With 182 enzymes: SgfI * January 16, 2009 15:30 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5395 278 Size: 53 83 186 936 3859 2468 Fragments arranged by size: 3859 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4928 1937 Size: 2991 4594 Acc65I G'GTAC_C Cuts at: 2112 4906 2112 Size: 2794 4791 AclI AA'CG_TT Cuts at: 1563 5712 6085 1563 Size: 4149 373 3063 Fragments arranged by size: 4149 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2325 2512 3762 3828 3837 3996 4365 4704 Size: 693 187 1250 66 9 159 369 339 Cuts at: 4704 4773 5648 7032 7300 1632 Size: 69 875 1384 268 1917 Fragments arranged by size: 1917 1384 1250 875 693 369 339 268 187 159 69 66 9 AflII C'TTAA_G Cuts at: 820 1017 2258 820 Size: 197 1241 6147 Fragments arranged by size: 6147 1241 197 AflIII A'CryG_T Cuts at: 1203 2825 6484 7461 1203 Size: 1622 3659 977 1327 Fragments arranged by size: 3659 1622 1327 977 AgeI A'CCGG_T Cuts at: 6416 6416 Size: 7585 AhdI GACnn_n'nnGTC Cuts at: 6314 6314 Size: 7585 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7585 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 4567 1184 Size: 3383 4202 AlwNI CAG_nnn'CTG Cuts at: 5328 6900 5328 Size: 1572 6013 ApaLI G'TGCA_C Cuts at: 5642 6798 5642 Size: 1156 6429 ApoI r'AATT_y Cuts at: 1493 2914 3656 4523 4894 5120 5272 7381 1493 Size: 1421 742 867 371 226 152 2109 1697 Fragments arranged by size: 2109 1697 1421 867 742 371 226 152 AseI AT'TA_AT Cuts at: 160 1032 3852 4533 6139 160 Size: 872 2820 681 1606 1606 Fragments arranged by size: 2820 1606 1606 872 681 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7585 AvaI C'yCGr_G Cuts at: 1870 1943 3667 4910 1870 Size: 73 1724 1243 4545 Fragments arranged by size: 4545 1724 1243 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 4473 4506 4473 Size: 33 7552 BamHI G'GATC_C Cuts at: 3385 3821 4195 4915 5282 3385 Size: 436 374 720 367 5688 Fragments arranged by size: 5688 720 436 374 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2112 4906 6361 618 Size: 325 217 627 325 2794 1455 1842 Fragments arranged by size: 2794 1842 1455 627 325 325 217 BanII G_rGCy'C Cuts at: 1391 4904 1391 Size: 3513 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2720 3750 4692 928 Size: 971 821 1030 942 3821 Fragments arranged by size: 3821 1030 971 942 821 BbvCI CC'TCA_GC Cuts at: 4236 4236 Size: 7585 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5776 5810 1903 Size: 34 3839 34 3678 Fragments arranged by size: 3839 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2214 4021 4474 5477 6687 2214 Size: 1807 453 1003 1210 3112 Fragments arranged by size: 3112 1807 1210 1003 453 BclI T'GATC_A Cuts at: 1548 2970 1548 Size: 1422 6163 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2802 3870 6196 Size: 107 122 71 631 605 1130 1068 2326 Cuts at: 6196 136 Size: 1525 Fragments arranged by size: 2326 1525 1130 1068 631 605 122 107 71 BglII A'GATC_T Cuts at: 3445 4172 7580 3445 Size: 727 3408 3450 Fragments arranged by size: 3450 3408 727 BlpI GC'TnA_GC Cuts at: 5002 5002 Size: 7585 Bme1580I G_kGCm'C Cuts at: 1790 5646 6802 1790 Size: 3856 1156 2573 Fragments arranged by size: 3856 2573 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7585 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2245 5316 6274 Size: 416 53 83 399 24 828 3071 958 Cuts at: 6274 442 Size: 1753 Fragments arranged by size: 3071 1753 958 828 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7585 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4404 6245 1239 Size: 3165 1841 2579 Fragments arranged by size: 3165 2579 1841 Bpu10I CC'TnA_GC Cuts at: 4157 4236 4157 Size: 79 7506 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3790 4424 5710 6575 6873 3790 Size: 634 1286 865 298 4502 Fragments arranged by size: 4502 1286 865 634 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2543 6248 882 Size: 617 1044 3705 2219 Fragments arranged by size: 3705 2219 1044 617 BsaAI yAC'GTr Cuts at: 493 1204 2826 3628 4882 5376 493 Size: 711 1622 802 1254 494 2702 Fragments arranged by size: 2702 1622 1254 802 711 494 BsaBI GATnn'nnATC Cuts at: 2898 4194 7579 2898 Size: 1296 3385 2904 Fragments arranged by size: 3385 2904 1296 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 5392 5774 6409 275 Size: 53 83 186 936 3859 382 635 1451 Fragments arranged by size: 3859 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2094 6018 6416 6690 6837 7248 2094 Size: 3924 398 274 147 411 2431 Fragments arranged by size: 3924 2431 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 7360 Fragments arranged by size: 7360 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 4005 4614 1186 Size: 2819 609 4157 Fragments arranged by size: 4157 2819 609 BseYI C'CCAG_C Cuts at: 2055 6788 7284 2055 Size: 4733 496 2356 Fragments arranged by size: 4733 2356 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 7012 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5035 5796 6824 1540 Size: 275 186 3034 761 1028 2301 Fragments arranged by size: 3034 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3627 5646 5731 6802 3627 Size: 2019 85 1071 4410 Fragments arranged by size: 4410 2019 1071 85 BsmI GAATG_Cn' Cuts at: 5096 5189 5096 Size: 93 7492 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3627 4904 5646 5731 6802 1391 Size: 399 1837 1277 742 85 1071 2174 Fragments arranged by size: 2174 1837 1277 1071 742 399 85 BspEI T'CCGG_A Cuts at: 2094 7248 2094 Size: 5154 2431 BspHI T'CATG_A Cuts at: 2832 5474 7204 2832 Size: 2642 1730 3213 Fragments arranged by size: 3213 2642 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3490 4535 4940 844 Size: 2646 1045 405 3489 Fragments arranged by size: 3489 2646 1045 405 BsrBI CCG'CTC Cuts at: 1394 1823 2039 5472 1394 Size: 429 216 3433 3507 Fragments arranged by size: 3507 3433 429 216 BsrDI GCAATG_nn' Cuts at: 65 1248 3335 4837 6080 6254 65 Size: 1183 2087 1502 1243 174 1396 Fragments arranged by size: 2087 1502 1396 1243 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 6229 6416 1700 Size: 110 4419 187 2869 Fragments arranged by size: 4419 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 3452 96 Size: 3356 4229 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7585 BssSI C'ACGA_G Cuts at: 5645 6657 5645 Size: 1012 6573 BstAPI GCAn_nnn'nTGC Cuts at: 794 3464 794 Size: 2670 4915 BstBI TT'CG_AA Cuts at: 2007 6389 2007 Size: 4382 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7585 BstXI CCAn_nnnn'nTGG Cuts at: 3686 3686 Size: 7585 BstYI r'GATC_y Cuts at: 1972 2044 3385 3445 3821 4172 4195 4348 4915 Size: 72 1341 60 376 351 23 153 567 Cuts at: 4915 5282 5667 5684 7136 7222 7234 7580 1972 Size: 367 385 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1341 567 385 376 367 351 346 153 86 72 60 23 17 12 Bsu36I CC'TnA_GG Cuts at: 2929 2929 Size: 7585 BtgI C'CryG_G Cuts at: 513 1065 2022 513 Size: 552 957 6076 Fragments arranged by size: 6076 957 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2053 487 Size: 683 56 827 6019 Fragments arranged by size: 6019 827 683 56 BtsI GCAGTG_nn' Cuts at: 2398 3829 4674 5101 5895 5915 2398 Size: 1431 845 427 794 20 4068 Fragments arranged by size: 4068 1431 845 794 427 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7585 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7550 DraI TTT'AAA Cuts at: 3216 4889 3216 Size: 1673 5912 DraIII CAC_nnn'GTG Cuts at: 3896 3896 Size: 7585 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4667 6592 809 Size: 507 3351 1925 1802 Fragments arranged by size: 3351 1925 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2681 5032 5921 Size: 54 1425 50 275 18 851 2351 889 Cuts at: 5921 7550 8 Size: 1629 43 Fragments arranged by size: 2351 1629 1425 889 851 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5032 1537 Size: 275 3220 4090 Fragments arranged by size: 4090 3220 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4724 5515 1353 Size: 3371 791 3423 Fragments arranged by size: 3423 3371 791 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2042 2049 2057 6172 6546 6692 Size: 1064 138 453 7 8 4115 374 146 Cuts at: 6692 387 Size: 1280 Fragments arranged by size: 4115 1280 1064 453 374 146 138 8 7 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2325 2512 3762 3828 3837 3996 4365 Size: 393 693 187 1250 66 9 159 369 Cuts at: 4365 4404 4704 4773 5648 6245 7032 7300 1239 Size: 39 300 69 875 597 787 268 1524 Fragments arranged by size: 1524 1250 875 787 693 597 393 369 300 268 187 159 69 66 39 9 EcoO109I rG'GnC_Cy Cuts at: 2690 4547 2690 Size: 1857 5728 EcoRI G'AATT_C Cuts at: 4523 4894 5272 4523 Size: 371 378 6836 Fragments arranged by size: 6836 378 371 EcoRV GAT'ATC Cuts at: 3062 4105 3062 Size: 1043 6542 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2709 3767 2709 Size: 1058 6527 FspI TGC'GCA Cuts at: 6091 6091 Size: 7585 HaeII r_GCGC'y Cuts at: 1678 6732 1678 Size: 5054 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2176 2208 2237 2269 2332 Size: 32 135 32 604 32 29 32 63 Cuts at: 2332 2364 3911 3943 4157 4189 4394 4426 6226 Size: 32 1547 32 214 32 205 32 1800 Cuts at: 6226 6258 6300 6332 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1800 1547 604 214 205 135 63 42 32 32 32 32 32 32 32 32 32 32 29 HincII GTy'rAC Cuts at: 669 1657 1938 4043 4929 5175 669 Size: 988 281 2105 886 246 3079 Fragments arranged by size: 3079 2105 988 886 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7585 HpaI GTT'AAC Cuts at: 5175 5175 Size: 7585 KpnI G_GTAC'C Cuts at: 2116 4910 2116 Size: 2794 4791 MfeI C'AATT_G Cuts at: 2502 5184 2502 Size: 2682 4903 MluI A'CGCG_T Cuts at: 7461 7461 Size: 7585 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2291 3967 4458 4500 4602 6699 6883 1305 Size: 986 1676 491 42 102 2097 184 2007 Fragments arranged by size: 2097 2007 1676 986 491 184 102 42 MscI TGG'CCA Cuts at: 10 64 1489 1832 2683 10 Size: 54 1425 343 851 4912 Fragments arranged by size: 4912 1425 851 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2339 4257 5543 5902 6061 518 Size: 648 1173 1918 1286 359 159 2042 Fragments arranged by size: 2042 1918 1286 1173 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2059 2328 4793 5001 5678 6826 7071 1606 Size: 453 269 2465 208 677 1148 245 2120 Fragments arranged by size: 2465 2120 1148 677 453 269 245 208 NcoI C'CATG_G Cuts at: 513 1065 2022 513 Size: 552 957 6076 Fragments arranged by size: 6076 957 552 NdeI CA'TA_TG Cuts at: 387 4711 387 Size: 4324 3261 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7585 NotI GC'GGCC_GC Cuts at: 5032 5032 Size: 7585 NruI TCG'CGA Cuts at: 2072 2072 Size: 7585 NspI r_CATG'y Cuts at: 1132 1229 4943 6488 1132 Size: 97 3714 1545 2229 Fragments arranged by size: 3714 2229 1545 97 PacI TTA_AT'TAA Cuts at: 4533 4533 Size: 7585 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7585 PciI A'CATG_T Cuts at: 6484 6484 Size: 7585 PflMI CCAn_nnn'nTGG Cuts at: 1108 2559 1108 Size: 1451 6134 PfoI T'CCnGG_A Cuts at: 2206 2206 Size: 7585 PmeI GTTT'AAAC Cuts at: 4889 4889 Size: 7585 PmlI CAC'GTG Cuts at: 3628 3628 Size: 7585 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5654 5686 7167 7199 5654 Size: 32 1481 32 6040 Fragments arranged by size: 6040 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2690 4547 2690 Size: 1857 5728 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7585 PsiI TTA'TAA Cuts at: 2748 3756 5155 2748 Size: 1008 1399 5178 Fragments arranged by size: 5178 1399 1008 PspXI vC'TCGA_Gb Cuts at: 3667 3667 Size: 7585 PstI C_TGCA'G Cuts at: 830 3487 4937 830 Size: 2657 1450 3478 Fragments arranged by size: 3478 2657 1450 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7585 PvuII CAG'CTG Cuts at: 2328 4793 2328 Size: 2465 5120 SalI G'TCGA_C Cuts at: 1936 4927 1936 Size: 2991 4594 SbfI CC_TGCA'GG Cuts at: 4937 4937 Size: 7585 ScaI AGT'ACT Cuts at: 4425 5833 7545 4425 Size: 1408 1712 4465 Fragments arranged by size: 4465 1712 1408 SexAI A'CCwGG_T Cuts at: 2108 2108 Size: 7585 SfcI C'TryA_G Cuts at: 826 1045 3483 4933 6068 6749 6940 826 Size: 219 2438 1450 1135 681 191 1471 Fragments arranged by size: 2438 1471 1450 1135 681 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 2802 2802 Size: 7585 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7585 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7585 SmaI CCC'GGG Cuts at: 1872 4912 1872 Size: 3040 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2258 3667 3769 4439 5689 6590 Size: 197 926 315 1409 102 670 1250 901 Cuts at: 6590 6852 820 Size: 262 1553 Fragments arranged by size: 1553 1409 1250 926 901 670 315 262 197 102 SnaBI TAC'GTA Cuts at: 493 4882 5376 493 Size: 4389 494 2702 Fragments arranged by size: 4389 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 7585 SphI G_CATG'C Cuts at: 1132 4943 1132 Size: 3811 3774 SspI AAT'ATT Cuts at: 5 52 3534 3850 5509 5 Size: 47 3482 316 1659 2081 Fragments arranged by size: 3482 2081 1659 316 47 StuI AGG'CCT Cuts at: 1517 2796 1517 Size: 1279 6306 StyI C'CwwG_G Cuts at: 513 1065 2022 2635 2684 3360 5024 513 Size: 552 957 613 49 676 1664 3074 Fragments arranged by size: 3074 1664 957 676 613 552 49 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2069 4785 5612 5629 5782 5967 6412 Size: 1255 489 2716 827 17 153 185 445 Cuts at: 6412 325 Size: 1498 Fragments arranged by size: 2716 1498 1255 827 489 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3408 3452 4019 Size: 274 80 33 51 1443 1431 44 567 Cuts at: 4019 4423 4432 4714 5831 7543 96 Size: 404 9 282 1117 1712 138 Fragments arranged by size: 1712 1443 1431 1117 567 404 282 274 138 80 51 44 33 9 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 7545 7577 7545 Size: 32 7553 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7585 XbaI T'CTAG_A Cuts at: 4921 4921 Size: 7585 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2307 3851 4396 2307 Size: 1544 545 5496 Fragments arranged by size: 5496 1544 545 XhoI C'TCGA_G Cuts at: 1943 3667 1943 Size: 1724 5861 XmaI C'CCGG_G Cuts at: 1870 4910 1870 Size: 3040 4545 XmnI GAAnn'nnTTC Cuts at: 3226 5714 3226 Size: 2488 5097 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5393 276 Size: 53 83 186 936 3859 2468 Fragments arranged by size: 3859 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NruI NspI PacI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspXI PstI PvuI PvuII SalI SbfI ScaI SexAI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BbeI BfrBI BplI BsiWI BsmBI BstZ17I EcoICRI EcoNI FseI FspAI KasI NaeI NarI NgoMIV NsiI PspOMI PsrI RsrII SacI SacII SanDI SapI SfoI SrfI SwaI