Restriction map for
pFN21ASDA0529
(Linear) (Six-base) MAPSORT of: KIAA0529.seq Check: 2320 from: 1 to: 2856
KIAA0529.orf
With 182 enzymes: SgfI *
October 24, 2006 15:12 ..
Acc65I G'GTAC_C
Cuts at: 0 89 2856
Size: 89 2767
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 302 489 1739 1805 1814 1973 2342 2681
Size: 302 187 1250 66 9 159 369 339
Cuts at: 2681 2750 2856
Size: 69 106
Fragments arranged by size:
1250 369 339 302 187 159 106 69
66 9
AflII C'TTAA_G
Cuts at: 0 235 2856
Size: 235 2621
AflIII A'CryG_T
Cuts at: 0 802 2856
Size: 802 2054
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2544 2856
Size: 2544 312
ApoI r'AATT_y
Cuts at: 0 891 1633 2500 2856
Size: 891 742 867 356
Fragments arranged by size:
891 867 742 356
AseI AT'TA_AT
Cuts at: 0 1829 2510 2856
Size: 1829 681 346
Fragments arranged by size:
1829 681 346
AvaI C'yCGr_G
Cuts at: 0 1644 2856
Size: 1644 1212
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 2450 2483 2856
Size: 2450 33 373
Fragments arranged by size:
2450 373 33
BamHI G'GATC_C
Cuts at: 0 1362 1798 2172 2856
Size: 1362 436 374 684
Fragments arranged by size:
1362 684 436 374
BanI G'GyrC_C
Cuts at: 0 89 2856
Size: 89 2767
BbsI GAAGACnn'nnnn_
Cuts at: 0 697 1727 2669 2856
Size: 697 1030 942 187
Fragments arranged by size:
1030 942 697 187
BbvCI CC'TCA_GC
Cuts at: 0 2213 2856
Size: 2213 643
BciVI GTATCCnnnnn_n'
Cuts at: 0 191 1998 2451 2856
Size: 191 1807 453 405
Fragments arranged by size:
1807 453 405 191
BclI T'GATC_A
Cuts at: 0 947 2856
Size: 947 1909
BglI GCCn_nnn'nGGC
Cuts at: 0 779 1847 2856
Size: 779 1068 1009
Fragments arranged by size:
1068 1009 779
BglII A'GATC_T
Cuts at: 0 1422 2149 2856
Size: 1422 727 707
Fragments arranged by size:
1422 727 707
BmrI ACTGGGnnnn_n'
Cuts at: 0 222 2856
Size: 222 2634
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2381 2856
Size: 2381 475
Bpu10I CC'TnA_GC
Cuts at: 0 2134 2213 2856
Size: 2134 79 643
Fragments arranged by size:
2134 643 79
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1767 2401 2856
Size: 1767 634 455
Fragments arranged by size:
1767 634 455
BsaI GGTCTCn'nnnn_
Cuts at: 0 520 2856
Size: 520 2336
BsaAI yAC'GTr
Cuts at: 0 803 1605 2856
Size: 803 802 1251
Fragments arranged by size:
1251 803 802
BsaBI GATnn'nnATC
Cuts at: 0 875 2171 2856
Size: 875 1296 685
Fragments arranged by size:
1296 875 685
BsaWI w'CCGG_w
Cuts at: 0 71 2856
Size: 71 2785
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1982 2591 2856
Size: 1982 609 265
Fragments arranged by size:
1982 609 265
BseYI C'CCAG_C
Cuts at: 0 32 2856
Size: 32 2824
BsiHKAI G_wGCw'C
Cuts at: 0 1604 2856
Size: 1604 1252
Bsp1286I G_dGCh'C
Cuts at: 0 1604 2856
Size: 1604 1252
BspEI T'CCGG_A
Cuts at: 0 71 2856
Size: 71 2785
BspHI T'CATG_A
Cuts at: 0 809 2856
Size: 809 2047
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1467 2512 2856
Size: 1467 1045 344
Fragments arranged by size:
1467 1045 344
BsrBI CCG'CTC
Cuts at: 0 16 2856
Size: 16 2840
BsrDI GCAATG_nn'
Cuts at: 0 1312 2814 2856
Size: 1312 1502 42
Fragments arranged by size:
1502 1312 42
BsrGI T'GTAC_A
Cuts at: 0 1429 2856
Size: 1429 1427
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1441 2856
Size: 1441 1415
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1663 2856
Size: 1663 1193
BstYI r'GATC_y
Cuts at: 0 21 1362 1422 1798 2149 2172 2325 2856
Size: 21 1341 60 376 351 23 153 531
Fragments arranged by size:
1341 531 376 351 153 60 23 21
Bsu36I CC'TnA_GG
Cuts at: 0 906 2856
Size: 906 1950
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 30 2856
Size: 30 2826
BtsI GCAGTG_nn'
Cuts at: 0 375 1806 2651 2856
Size: 375 1431 845 205
Fragments arranged by size:
1431 845 375 205
DraI TTT'AAA
Cuts at: 0 1193 2856
Size: 1193 1663
DraIII CAC_nnn'GTG
Cuts at: 0 1873 2856
Size: 1873 983
DrdI GACnn_nn'nnGTC
Cuts at: 0 2644 2856
Size: 2644 212
EaeI y'GGCC_r
Cuts at: 0 658 2856
Size: 658 2198
EarI CTCTTCn'nnn_
Cuts at: 0 2701 2856
Size: 2701 155
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 19 26 34 2856
Size: 19 7 8 2822
Fragments arranged by size:
2822 19 8 7
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 302 489 1739 1805 1814 1973 2342 2381
Size: 302 187 1250 66 9 159 369 39
Cuts at: 2381 2681 2750 2856
Size: 300 69 106
Fragments arranged by size:
1250 369 302 300 187 159 106 69
66 39 9
EcoO109I rG'GnC_Cy
Cuts at: 0 667 2524 2856
Size: 667 1857 332
Fragments arranged by size:
1857 667 332
EcoRI G'AATT_C
Cuts at: 0 2500 2856
Size: 2500 356
EcoRV GAT'ATC
Cuts at: 0 1039 2082 2856
Size: 1039 1043 774
Fragments arranged by size:
1043 1039 774
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 686 1744 2856
Size: 686 1058 1112
Fragments arranged by size:
1112 1058 686
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 153 185 214 246 309 341 1888 1920
Size: 153 32 29 32 63 32 1547 32
Cuts at: 1920 2134 2166 2371 2403 2856
Size: 214 32 205 32 453
Fragments arranged by size:
1547 453 214 205 153 63 32 32
32 32 32 32 29
HincII GTy'rAC
Cuts at: 0 2020 2856
Size: 2020 836
KpnI G_GTAC'C
Cuts at: 0 93 2856
Size: 93 2763
MfeI C'AATT_G
Cuts at: 0 479 2856
Size: 479 2377
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 268 1944 2435 2477 2579 2856
Size: 268 1676 491 42 102 277
Fragments arranged by size:
1676 491 277 268 102 42
MscI TGG'CCA
Cuts at: 0 660 2856
Size: 660 2196
MslI CAynn'nnrTG
Cuts at: 0 316 2234 2856
Size: 316 1918 622
Fragments arranged by size:
1918 622 316
MspA1I CmG'CkG
Cuts at: 0 36 305 2770 2856
Size: 36 269 2465 86
Fragments arranged by size:
2465 269 86 36
NdeI CA'TA_TG
Cuts at: 0 2688 2856
Size: 2688 168
NruI TCG'CGA
Cuts at: 0 49 2856
Size: 49 2807
PacI TTA_AT'TAA
Cuts at: 0 2510 2856
Size: 2510 346
PflMI CCAn_nnn'nTGG
Cuts at: 0 536 2856
Size: 536 2320
PfoI T'CCnGG_A
Cuts at: 0 183 2856
Size: 183 2673
PmlI CAC'GTG
Cuts at: 0 1605 2856
Size: 1605 1251
PpuMI rG'GwC_Cy
Cuts at: 0 667 2524 2856
Size: 667 1857 332
Fragments arranged by size:
1857 667 332
PsiI TTA'TAA
Cuts at: 0 725 1733 2856
Size: 725 1008 1123
Fragments arranged by size:
1123 1008 725
PspXI vC'TCGA_Gb
Cuts at: 0 1644 2856
Size: 1644 1212
PstI C_TGCA'G
Cuts at: 0 1464 2856
Size: 1464 1392
PvuII CAG'CTG
Cuts at: 0 305 2770 2856
Size: 305 2465 86
Fragments arranged by size:
2465 305 86
ScaI AGT'ACT
Cuts at: 0 2402 2856
Size: 2402 454
SexAI A'CCwGG_T
Cuts at: 0 85 2856
Size: 85 2771
SfcI C'TryA_G
Cuts at: 0 1460 2856
Size: 1460 1396
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 779 2856
Size: 779 2077
SmlI C'TyrA_G
Cuts at: 0 235 1644 1746 2416 2856
Size: 235 1409 102 670 440
Fragments arranged by size:
1409 670 440 235 102
SspI AAT'ATT
Cuts at: 0 1511 1827 2856
Size: 1511 316 1029
Fragments arranged by size:
1511 1029 316
StuI AGG'CCT
Cuts at: 0 773 2856
Size: 773 2083
StyI C'CwwG_G
Cuts at: 0 612 661 1337 2856
Size: 612 49 676 1519
Fragments arranged by size:
1519 676 612 49
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 46 2762 2856
Size: 46 2716 94
Fragments arranged by size:
2716 94 46
TatI w'GTAC_w
Cuts at: 0 1385 1429 1996 2400 2409 2691 2856
Size: 1385 44 567 404 9 282 165
Fragments arranged by size:
1385 567 404 282 165 44 9
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 284 1828 2373 2856
Size: 284 1544 545 483
Fragments arranged by size:
1544 545 483 284
XhoI C'TCGA_G
Cuts at: 0 1644 2856
Size: 1644 1212
XmnI GAAnn'nnTTC
Cuts at: 0 1203 2856
Size: 1203 1653
Enzymes that do cut:
Acc65I AcuI AflII AflIII AlfI ApoI AseI AvaI
BaeI BamHI BanI BbsI BbvCI BciVI BclI BglI
BglII BmrI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaWI BseRI BseYI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrGI BstAPI BstXI BstYI Bsu36I BtgZI
BtsI DraI DraIII DrdI EaeI EarI EciI Eco57MI
EcoO109I EcoRI EcoRV FalI Hin4I HincII KpnI MfeI
MmeI MscI MslI MspA1I NdeI NruI PacI PflMI
PfoI PmlI PpuMI PsiI PspXI PstI PvuII ScaI
SexAI SfcI SfiI SmlI SspI StuI StyI TaqII
TatI XcmI XhoI XmnI
Enzymes that do not cut:
AarI AatII AccI AclI AfeI AgeI AhdI AleI
AloI AlwNI ApaI ApaLI AscI AsiSI AvrII BanII
BbeI BcgI BfrBI BlpI Bme1580I BmgBI BmtI BplI
BsaHI BsaXI BsgI BsiEI BsiWI BsmI BsmBI BsrFI
BssHII BssSI BstBI BstEII BstZ17I BtgI ClaI CspCI
EagI EcoICRI EcoNI FseI FspI FspAI HaeII HindIII
HpaI KasI MluI NaeI NarI NcoI NgoMIV NheI
NotI NsiI NspI PasI PciI PmeI PpiI PshAI
PspOMI PsrI PvuI RsrII SacI SacII SalI SanDI
SapI SbfI SfoI SgfI SgrAI SmaI SnaBI SpeI
SphI SrfI SwaI TstI Tth111I XbaI XmaI ZraI