Restriction map for
pFN21ASDA0470
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# NO CUT ENZYMES ( 86 enzymes) #
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AatII Acc65I AccII AflIII AgeI
AhdI Alw44I AocI ApaI ApaLI
AscI AseI AsnI Asp718I AspEI
AspI AvrII BbeI BbrPI BcgI
BglI BlnI BsiWI BspDI BspLU11I
BspMI BsrBI BsrFI BssHII BstBI
BstEII BstPI BstUI Bsu36I Cfr10I
ClaI CpoI EagI Eam1105I EclXI
Eco52I Eco81I EcoO65I EcoRV FauI
FseI HpaI KasI KpnI KspI
MfeI MluI MunI MvnI NaeI
NarI NdeI NgoMIV NotI NruI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PshAI PshBI
PvuI RsrII SacII SalI SbfI
SexAI SfiI SfoI SfuI SgrAI
SnaBI Sse8387I SwaI Tth111I XcmI
XhoI
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# ONE CUT ENZYMES ( 56 enzymes) #
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AccIII 219 TCCGGA T^CCGGA
AclI 1448 AACGTT AA^CGTT
AcyI 1202 GACGCC G[GA]^CG[CT]C
Aor51HI 3406 AGCGCT AGC^GCT
Asp700I 2257 GAACTTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AviII 3014 TGCGCA TGC^GCA
BalI 4236 TGGCCA TGG^CCA
BamHI 2352 GGATCC G^GATCC
BanI 981 GGCACC G^G[CT][GA]CC
BciVI 3088 GTATCC GTATCC(6/5)
BlpI 3157 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 3157 GCTCAGC GC^T[ACGT]AGC
BsaAI 2429 TACGTG [CT]AC^GT[GA]
BsaHI 1202 GACGCC G[GA]^CG[CT]C
BsaI 884 GAGACC GGTCTC(1/5)
BseAI 219 TCCGGA T^CCGGA
BsiEI 3771 CGGTCG CG[GA][CT]^CG
BsmBI 1629 GAGACG CGTCTC(1/5)
BsmI 4296 GAATGC GAATGC(1/-1)
Bsp1407I 2895 TGTACA T^GTACA
BspEI 219 TCCGGA T^CCGGA
BspHI 777 TCATGA T^CATGA
BsrGI 2895 TGTACA T^GTACA
BssSI 56 CACGAG CACGAG(-5/-1)
CelII 3157 GCTCAGC GC^T[ACGT]AGC
DraII 311 GGGTCCC [GA]G^G[ACGT]CC[CT]
DraIII 2114 CACAAAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 3337 GACTCCAAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 4236 TGGCCA [CT]^GGCC[GA]
Eco47III 3406 AGCGCT AGC^GCT
EcoO109I 311 GGGTCCC [GA]G^G[ACGT]CC[CT]
EcoRI 739 GAATTC G^AATTC
EcoT22I 3679 ATGCAT ATGCA^T
FspI 3014 TGCGCA TGC^GCA
Hin1I 1202 GACGCC G[GA]^CG[CT]C
HincII 2559 GTCAAC GT[CT]^[GA]AC
HindII 2559 GTCAAC GT[CT]^[GA]AC
HindIII 3035 AAGCTT A^AGCTT
MluNI 4236 TGGCCA TGG^CCA
MroI 219 TCCGGA T^CCGGA
MscI 4236 TGGCCA TGG^CCA
NcoI 491 CCATGG C^CATGG
NsiI 3679 ATGCAT ATGCA^T
PpuMI 311 GGGTCCC [GA]G^G[AT]CC[CT]
Psp1406I 1448 AACGTT AA^CGTT
RcaI 777 TCATGA T^CATGA
SacI 91 GAGCTC GAGCT^C
SapI 4255 GCTCTTC GCTCTTC(1/4)
SmaI 4215 CCCGGG CCC^GGG
SphI 828 GCATGC GCATG^C
SspBI 2895 TGTACA T^GTACA
SspI 3948 AATATT AAT^ATT
StuI 1306 AGGCCT AGG^CCT
XmaCI 4215 CCCGGG C^CCGGG
XmaI 4215 CCCGGG C^CCGGG
XmnI 2257 GAACTTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AflII 328 CTTAAG C^TTAAG
1197 CTTAAG
AspHI 91 GAGCTC G[AT]GC[AT]^C
3020 GAGCAC
AvaI 3536 CTCGGG C^[CT]CG[GA]G
4215 CCCGGG
BcnI 4215 CCCGG CC^[GC]GG
4216 CCGGG
BfrI 328 CTTAAG C^TTAAG
1197 CTTAAG
BsaBI 274 GATACAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1285 GATGACAATC
BsiHKAI 91 GAGCTC G[AT]GC[AT]^C
3020 GAGCAC
BsoBI 3536 CTCGGG C^[CT]CG[GA]G
4215 CCCGGG
BsrDI 3531 CATTGC GCAATG(2/0)
3418 GCAATG
Bst1107I 1762 GTATAC GTA^TAC
4322 GTATAC
BstXI 612 CCATGAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2693 CCACAGATGTGG
BstZ17I 1762 GTATAC GTA^TAC
4322 GTATAC
DraI 2047 TTTAAA TTT^AAA
3955 TTTAAA
DsaI 491 CCATGG C^C[GA][CT]GG
513 CCGTGG
MamI 274 GATACAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1285 GATGACAATC
NciI 4215 CCCGG CC^[GC]GG
4216 CCGGG
NheI 1008 GCTAGC G^CTAGC
1609 GCTAGC
NspI 828 GCATGC [GA]CATG^[CT]
2892 GCATGT
PflMI 523 CCATTATATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4228 CCAACACTTGG
PstI 668 CTGCAG CTGCA^G
1364 CTGCAG
ScaI 687 AGTACT AGT^ACT
1585 AGTACT
SpeI 3276 ACTAGT A^CTAGT
3427 ACTAGT
Van91I 523 CCATTATATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4228 CCAACACTTGG
XbaI 2415 TCTAGA T^CTAGA
3220 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 92 enzymes) #
#########################################
AccI AciI AcsI AfaI AluI
AlwI AlwNI ApoI AvaII BanII
BbsI BbvCI BbvI BclI BfaI
BglII BmyI BpmI BpuAI BsaJI
BsaWI BseRI BsgI BsiYI BslI
BsmAI BsmFI Bsp1286I BsrI BssKI
BstAPI BstF5I BstNI BstYI Cac8I
CfoI Cfr13I DdeI DpnII EarI
EcoNI EcoRII EcoT14I FbaI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MwoI
NdeII NlaIII NlaIV PleI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XhoII