(Circular) (Six-base) MAPSORT of: pFN21ASDA0470.seq Check: 8064 from: 1 to: 9109 pFN21ASDA0470 9109 bp With 182 enzymes: SgfI * January 16, 2009 15:30 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 6919 278 Size: 53 83 186 936 5383 2468 Fragments arranged by size: 5383 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2174 3786 4788 6346 6452 1937 Size: 237 1612 1002 1558 106 4594 Fragments arranged by size: 4594 1612 1558 1002 237 106 Acc65I G'GTAC_C Cuts at: 6430 6430 Size: 9109 AclI AA'CG_TT Cuts at: 1563 3472 7236 7609 1563 Size: 1909 3764 373 3063 Fragments arranged by size: 3764 3063 1909 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2361 2363 2382 2487 2508 2643 3743 4656 Size: 729 2 19 105 21 135 1100 913 Cuts at: 4656 5046 5127 5250 5385 7172 8556 8824 1632 Size: 390 81 123 135 1787 1384 268 1917 Fragments arranged by size: 1917 1787 1384 1100 913 729 390 268 135 135 123 105 81 21 19 2 AfeI AGC'GCT Cuts at: 5431 5431 Size: 9109 AflII C'TTAA_G Cuts at: 820 1017 2351 3220 820 Size: 197 1334 869 6709 Fragments arranged by size: 6709 1334 869 197 AflIII A'CryG_T Cuts at: 1203 8008 8985 1203 Size: 6805 977 1327 Fragments arranged by size: 6805 1327 977 AgeI A'CCGG_T Cuts at: 7940 7940 Size: 9109 AhdI GACnn_n'nnGTC Cuts at: 7838 7838 Size: 9109 AleI CACnn'nnGTG Cuts at: 1166 4721 1166 Size: 3555 5554 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 9109 AlwNI CAG_nnn'CTG Cuts at: 2459 3189 4526 5612 6088 6852 8424 2459 Size: 730 1337 1086 476 764 1572 3144 Fragments arranged by size: 3144 1572 1337 1086 764 730 476 ApaLI G'TGCA_C Cuts at: 7166 8322 7166 Size: 1156 7953 ApoI r'AATT_y Cuts at: 1493 2762 2870 3542 4099 4170 4748 5315 6311 Size: 1269 108 672 557 71 578 567 996 Cuts at: 6311 6418 6644 6796 8905 1493 Size: 107 226 152 2109 1697 Fragments arranged by size: 2109 1697 1269 996 672 578 567 557 226 152 108 107 71 AseI AT'TA_AT Cuts at: 160 1032 7663 160 Size: 872 6631 1606 Fragments arranged by size: 6631 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 9109 AvaI C'yCGr_G Cuts at: 1870 1943 5559 6238 6434 1870 Size: 73 3616 679 196 4545 Fragments arranged by size: 4545 3616 679 196 73 BamHI G'GATC_C Cuts at: 4375 6439 6806 4375 Size: 2064 367 6678 Fragments arranged by size: 6678 2064 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3004 6430 7885 618 Size: 325 217 627 1217 3426 1455 1842 Fragments arranged by size: 3426 1842 1455 1217 627 325 217 BanII G_rGCy'C Cuts at: 1391 2118 3181 3376 6280 6428 1391 Size: 727 1063 195 2904 148 4072 Fragments arranged by size: 4072 2904 1063 727 195 148 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2949 4014 5493 928 Size: 971 1050 1065 1479 4544 Fragments arranged by size: 4544 1479 1065 1050 971 BbvCI CC'TCA_GC Cuts at: 4164 4903 5383 4164 Size: 739 480 7890 Fragments arranged by size: 7890 739 480 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 7300 7334 1903 Size: 34 5363 34 3678 Fragments arranged by size: 5363 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5122 7001 8211 5122 Size: 1879 1210 6020 Fragments arranged by size: 6020 1879 1210 BclI T'GATC_A Cuts at: 1548 2932 3520 4855 1548 Size: 1384 588 1335 5802 Fragments arranged by size: 5802 1384 1335 588 BfrBI ATG'CAT Cuts at: 5704 5704 Size: 9109 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 7720 136 Size: 107 122 71 631 605 6048 1525 Fragments arranged by size: 6048 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3355 5542 5686 9104 3355 Size: 2187 144 3418 3360 Fragments arranged by size: 3418 3360 2187 144 BlpI GC'TnA_GC Cuts at: 5181 6526 5181 Size: 1345 7764 Bme1580I G_kGCm'C Cuts at: 1790 7170 8326 1790 Size: 5380 1156 2573 Fragments arranged by size: 5380 2573 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 9109 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4485 6171 6840 Size: 416 53 83 399 24 3068 1686 669 Cuts at: 6840 7798 442 Size: 958 1753 Fragments arranged by size: 3068 1753 1686 958 669 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 3035 3636 1056 Size: 1979 601 6529 Fragments arranged by size: 6529 1979 601 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3675 3872 3675 Size: 197 8912 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4074 4584 5887 7769 1239 Size: 2835 510 1303 1882 2579 Fragments arranged by size: 2835 2579 1882 1303 510 Bpu10I CC'TnA_GC Cuts at: 4164 4903 5383 4164 Size: 739 480 7890 Fragments arranged by size: 7890 739 480 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2955 3230 3713 3841 5802 6040 7234 8099 8397 Size: 275 483 128 1961 238 1194 865 298 Cuts at: 8397 2955 Size: 3667 Fragments arranged by size: 3667 1961 1194 865 483 298 275 238 128 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2901 7772 882 Size: 617 1402 4871 2219 Fragments arranged by size: 4871 2219 1402 617 BsaAI yAC'GTr Cuts at: 493 1204 4454 6406 6900 493 Size: 711 3250 1952 494 2702 Fragments arranged by size: 3250 2702 1952 711 494 BsaBI GATnn'nnATC Cuts at: 2301 3312 9103 2301 Size: 1011 5791 2307 Fragments arranged by size: 5791 2307 1011 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3226 6916 7298 7933 Size: 53 83 186 936 1693 3690 382 635 Cuts at: 7933 275 Size: 1451 Fragments arranged by size: 3690 1693 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2242 4966 5469 7542 7940 8214 8361 8772 2242 Size: 2724 503 2073 398 274 147 411 2579 Fragments arranged by size: 2724 2579 2073 503 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3986 4016 4116 4146 4624 Size: 30 165 30 2583 30 100 30 478 Cuts at: 4624 4654 1178 Size: 30 5633 Fragments arranged by size: 5633 2583 478 165 100 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3039 4007 4212 1186 Size: 1853 968 205 6083 Fragments arranged by size: 6083 1853 968 205 BseYI C'CCAG_C Cuts at: 2980 3173 3666 4259 8312 8808 2980 Size: 193 493 593 4053 496 3281 Fragments arranged by size: 4053 3281 593 496 493 193 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2492 2677 2865 3534 1163 Size: 573 756 185 188 669 6738 Fragments arranged by size: 6738 756 669 573 188 185 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5797 6559 7320 8348 1540 Size: 275 186 3796 762 761 1028 2301 Fragments arranged by size: 3796 2301 1028 762 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2118 5047 7170 7255 8326 2118 Size: 2929 2123 85 1071 2901 Fragments arranged by size: 2929 2901 2123 1071 85 BsmI GAATG_Cn' Cuts at: 6325 6620 6713 6325 Size: 295 93 8721 Fragments arranged by size: 8721 295 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3646 3646 Size: 9109 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2118 3181 3376 5047 6280 6428 7170 Size: 399 328 1063 195 1671 1233 148 742 Cuts at: 7170 7255 8326 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1671 1233 1071 1063 742 399 328 195 148 85 BspEI T'CCGG_A Cuts at: 2242 8772 2242 Size: 6530 2579 BspHI T'CATG_A Cuts at: 2800 6998 8728 2800 Size: 4198 1730 3181 Fragments arranged by size: 4198 3181 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 6464 844 Size: 5620 3489 BsrBI CCG'CTC Cuts at: 1394 1823 6996 1394 Size: 429 5173 3507 Fragments arranged by size: 5173 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5448 5553 7604 7778 65 Size: 1183 4200 105 2051 174 1396 Fragments arranged by size: 4200 2051 1396 1183 174 105 BsrFI r'CCGG_y Cuts at: 1700 1810 7753 7940 1700 Size: 110 5943 187 2869 Fragments arranged by size: 5943 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 4918 96 Size: 4822 4287 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 9109 BssSI C'ACGA_G Cuts at: 2079 7169 8181 2079 Size: 5090 1012 3007 Fragments arranged by size: 5090 3007 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2459 2852 5099 6122 794 Size: 1665 393 2247 1023 3781 Fragments arranged by size: 3781 2247 1665 1023 393 BstBI TT'CG_AA Cuts at: 2007 7913 2007 Size: 5906 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 9109 BstXI CCAn_nnnn'nTGG Cuts at: 2642 4723 2642 Size: 2081 7028 BstYI r'GATC_y Cuts at: 1972 3355 4375 5542 5686 6439 6806 7191 7208 Size: 1383 1020 1167 144 753 367 385 17 Cuts at: 7208 8660 8746 8758 9104 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1383 1167 1020 753 385 367 346 144 86 17 12 BstZ17I GTA'TAC Cuts at: 3787 6347 3787 Size: 2560 6549 BtgI C'CryG_G Cuts at: 513 1065 2514 2536 513 Size: 552 1449 22 7086 Fragments arranged by size: 7086 1449 552 22 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 8370 Fragments arranged by size: 8370 683 56 BtsI GCAGTG_nn' Cuts at: 2059 5610 5634 6625 7419 7439 2059 Size: 3551 24 991 794 20 3729 Fragments arranged by size: 3729 3551 991 794 24 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 9109 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3847 3882 529 Size: 35 3283 35 5756 Fragments arranged by size: 5756 3283 35 35 DraI TTT'AAA Cuts at: 4072 5980 6413 4072 Size: 1908 433 6768 Fragments arranged by size: 6768 1908 433 DraIII CAC_nnn'GTG Cuts at: 4142 4142 Size: 9109 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5366 8116 809 Size: 507 4050 2750 1802 Fragments arranged by size: 4050 2750 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 6259 6556 7445 Size: 54 1425 50 275 18 4429 297 889 Cuts at: 7445 9074 8 Size: 1629 43 Fragments arranged by size: 4429 1629 1425 889 297 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 6556 1537 Size: 275 4744 4090 Fragments arranged by size: 4744 4090 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2095 3069 3561 3666 5263 5514 5670 6285 Size: 742 974 492 105 1597 251 156 615 Cuts at: 6285 6372 7039 1353 Size: 87 667 3423 Fragments arranged by size: 3423 1597 974 742 667 615 492 251 156 105 87 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5872 7696 8070 8216 387 Size: 1064 138 4283 1824 374 146 1280 Fragments arranged by size: 4283 1824 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2116 2116 Size: 9109 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2361 2363 2382 2487 2508 2643 3743 Size: 393 729 2 19 105 21 135 1100 Cuts at: 3743 4074 4584 4656 5046 5127 5250 5385 5887 Size: 331 510 72 390 81 123 135 502 Cuts at: 5887 7172 7769 8556 8824 1239 Size: 1285 597 787 268 1524 Fragments arranged by size: 1524 1285 1100 787 729 597 510 502 393 390 331 268 135 135 123 105 81 72 21 19 2 EcoNI CCTnn'n_nnAGG Cuts at: 5142 5260 5869 5142 Size: 118 609 8382 Fragments arranged by size: 8382 609 118 EcoO109I rG'GnC_Cy Cuts at: 2335 2335 Size: 9109 EcoRI G'AATT_C Cuts at: 2762 6418 6796 2762 Size: 3656 378 5075 Fragments arranged by size: 5075 3656 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2366 2729 4386 2366 Size: 363 1657 7089 Fragments arranged by size: 7089 1657 363 FspI TGC'GCA Cuts at: 5039 7615 5039 Size: 2576 6533 HaeII r_GCGC'y Cuts at: 1678 2941 3190 5433 8256 1678 Size: 1263 249 2243 2823 2531 Fragments arranged by size: 2823 2531 2243 1263 249 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2672 2704 4796 4828 4832 Size: 32 135 32 1100 32 2092 32 4 Cuts at: 4832 4864 5078 5110 5371 5403 5497 5529 7750 Size: 32 214 32 261 32 94 32 2221 Cuts at: 7750 7782 7824 7856 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2221 2092 1100 261 214 135 94 42 32 32 32 32 32 32 32 32 32 32 4 HincII GTy'rAC Cuts at: 669 1657 1938 4584 6453 6699 669 Size: 988 281 2646 1869 246 3079 Fragments arranged by size: 3079 2646 1869 988 281 246 HindIII A'AGCT_T Cuts at: 748 5058 748 Size: 4310 4799 HpaI GTT'AAC Cuts at: 6699 6699 Size: 9109 KpnI G_GTAC'C Cuts at: 6434 6434 Size: 9109 MfeI C'AATT_G Cuts at: 6708 6708 Size: 9109 MluI A'CGCG_T Cuts at: 8985 8985 Size: 9109 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2077 8223 8407 1305 Size: 772 6146 184 2007 Fragments arranged by size: 6146 2007 772 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 6261 10 Size: 54 1425 343 4429 2858 Fragments arranged by size: 4429 2858 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2653 3144 3599 4721 7067 7426 7585 Size: 648 1487 491 455 1122 2346 359 159 Cuts at: 7585 518 Size: 2042 Fragments arranged by size: 2346 2042 1487 1122 648 491 455 359 159 MspA1I CmG'CkG Cuts at: 1606 2696 4907 5957 6109 6308 6525 7202 8350 Size: 1090 2211 1050 152 199 217 677 1148 Cuts at: 8350 8595 1606 Size: 245 2120 Fragments arranged by size: 2211 2120 1148 1090 1050 677 245 217 199 152 NcoI C'CATG_G Cuts at: 513 1065 2514 513 Size: 552 1449 7108 Fragments arranged by size: 7108 1449 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 9109 NheI G'CTAG_C Cuts at: 1052 3031 3632 1052 Size: 1979 601 6529 Fragments arranged by size: 6529 1979 601 NotI GC'GGCC_GC Cuts at: 6556 6556 Size: 9109 NsiI A_TGCA'T Cuts at: 5706 5706 Size: 9109 NspI r_CATG'y Cuts at: 1132 1229 2855 4919 6467 8012 1132 Size: 97 1626 2064 1548 1545 2229 Fragments arranged by size: 2229 2064 1626 1548 1545 97 PasI CC'CwG_GG Cuts at: 1348 2888 1348 Size: 1540 7569 PciI A'CATG_T Cuts at: 8008 8008 Size: 9109 PflMI CCAn_nnn'nTGG Cuts at: 1108 2552 6257 1108 Size: 1444 3705 3960 Fragments arranged by size: 3960 3705 1444 PfoI T'CCnGG_A Cuts at: 2962 3907 2962 Size: 945 8164 PmeI GTTT'AAAC Cuts at: 6413 6413 Size: 9109 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 7178 7210 8691 8723 7178 Size: 32 1481 32 7564 Fragments arranged by size: 7564 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2335 2335 Size: 9109 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 9109 PsiI TTA'TAA Cuts at: 3324 4497 6679 3324 Size: 1173 2182 5754 Fragments arranged by size: 5754 2182 1173 PstI C_TGCA'G Cuts at: 830 2695 3391 6461 830 Size: 1865 696 3070 3478 Fragments arranged by size: 3478 3070 1865 696 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 9109 PvuII CAG'CTG Cuts at: 2696 4907 5957 6109 6308 2696 Size: 2211 1050 152 199 5497 Fragments arranged by size: 5497 2211 1050 199 152 SacI G_AGCT'C Cuts at: 2118 2118 Size: 9109 SalI G'TCGA_C Cuts at: 1936 6451 1936 Size: 4515 4594 SanDI GG'GwC_CC Cuts at: 2335 2335 Size: 9109 SapI GCTCTTCn'nnn_ Cuts at: 6285 6285 Size: 9109 SbfI CC_TGCA'GG Cuts at: 6461 6461 Size: 9109 ScaI AGT'ACT Cuts at: 2712 3610 7357 9069 2712 Size: 898 3747 1712 2752 Fragments arranged by size: 3747 2752 1712 898 SfcI C'TryA_G Cuts at: 826 1045 2313 2691 2725 3190 3251 3387 4248 Size: 219 1268 378 34 465 61 136 861 Cuts at: 4248 5943 6457 7592 8273 8464 826 Size: 1695 514 1135 681 191 1471 Fragments arranged by size: 1695 1471 1268 1135 861 681 514 465 378 219 191 136 61 34 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 9109 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 9109 SmaI CCC'GGG Cuts at: 1872 6240 6436 1872 Size: 4368 196 4545 Fragments arranged by size: 4545 4368 196 SmlI C'TyrA_G Cuts at: 820 1017 1943 2351 2970 3209 3220 3692 3820 Size: 197 926 408 619 239 11 472 128 Cuts at: 3820 5817 6055 7213 8114 8376 820 Size: 1997 238 1158 901 262 1553 Fragments arranged by size: 1997 1553 1158 926 901 619 472 408 262 239 238 197 128 11 SnaBI TAC'GTA Cuts at: 493 6406 6900 493 Size: 5913 494 2702 Fragments arranged by size: 5913 2702 494 SpeI A'CTAG_T Cuts at: 152 5299 5450 152 Size: 5147 151 3811 Fragments arranged by size: 5147 3811 151 SphI G_CATG'C Cuts at: 1132 2855 6467 1132 Size: 1723 3612 3774 Fragments arranged by size: 3774 3612 1723 SspI AAT'ATT Cuts at: 5 52 5973 7033 5 Size: 47 5921 1060 2081 Fragments arranged by size: 5921 2081 1060 47 StuI AGG'CCT Cuts at: 1517 3331 1517 Size: 1814 7295 StyI C'CwwG_G Cuts at: 513 1065 2514 3627 5006 5364 6548 513 Size: 552 1449 1113 1379 358 1184 3074 Fragments arranged by size: 3074 1449 1379 1184 1113 552 358 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 7136 7153 7306 7491 7936 325 Size: 1255 5556 17 153 185 445 1498 Fragments arranged by size: 5556 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2317 2710 3111 Size: 274 80 33 51 1443 340 393 401 Cuts at: 3111 3608 4544 4918 6394 7355 9067 96 Size: 497 936 374 1476 961 1712 138 Fragments arranged by size: 1712 1476 1443 961 936 497 401 393 374 340 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3986 4018 4116 4148 4624 4656 9069 9101 3986 Size: 32 98 32 476 32 4413 32 3994 Fragments arranged by size: 4413 3994 476 98 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 9109 XbaI T'CTAG_A Cuts at: 4438 5243 6445 4438 Size: 805 1202 7102 Fragments arranged by size: 7102 1202 805 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 9109 XmaI C'CCGG_G Cuts at: 1870 6238 6434 1870 Size: 4368 196 4545 Fragments arranged by size: 4545 4368 196 XmnI GAAnn'nnTTC Cuts at: 4284 7238 4284 Size: 2954 6155 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 6917 276 Size: 53 83 186 936 5383 2468 Fragments arranged by size: 5383 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SanDI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI AvrII BaeI BbeI BsiWI Bsu36I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PspOMI PspXI PsrI RsrII SacII SexAI SfiI SfoI SrfI SwaI XcmI